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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Gels were washed and proteins were stained with Imperial Protein Stain (Thermo Fisher Scientific, Rockford, IL, USA) for 2 hours ...
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bioRxiv - Immunology 2019Quote: ... IL-2 mRNA amounts were determined with a SYBR Green PCR master mix from Life Technologies (4344463) relative to mRNA for β-2 microglobulin on a DNA Engine Opticon II System (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... The quantity of the RNA was determined on a Nanodrop ND-1000 (Thermo Scientific, Rockford, IL, USA). Reverse-transcriptase PCR was performed using the Multiscribe Reverse Transcriptase method (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... and eluted with imidazole (gradient 10–200 mM) and dialyzed (prod. no. 68100, Thermo Scientific, Rockford, IL). In the case of Sec14 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration of cells was measured using a Micro BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) and used to normalize cytokine levels with final concentrations adjusted to an equivalent of 25 μg of protein for comparison across experiments.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Total protein content in the homogenate was estimated using the BCA assay (Thermo Fisher Scientific, Rockford, IL), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The blots were then developed using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, Il, USA) and signals were captured by using high sensitive CCD camera-based imager (BioSpectrum Imaging System) ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of conditioned media were analyzed by ELISA (IL-1 beta Human ELISA Kit, Thermofisher KHC0011), using manufacturer protocol ...
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bioRxiv - Physiology 2021Quote: ... The total protein was inoculated with anti-HA magnetic beads at 4 C (Thermo Scientific, Rockford, IL) overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Chemiluminescent kit was a Supersignal West pico plus chemiluminescent substrate (34577) purchased from Thermo Scientific (Rockford, IL).
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bioRxiv - Synthetic Biology 2021Quote: The optical density was measured at 600 nm using a spectrophotometer (GENESYS 30, Thermo Scientific, IL, USA). The dry cell mass was obtained by multiplication of the optical density of culture broth with a pre-determined conversion factor ...
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bioRxiv - Pathology 2021Quote: ... hMADS adipocytes were differentiated in 2-well Lab-Tek Chamber Slides (Nalge Nunc International, Naperville, IL, USA), washed with PBS pH 7.4 and fixed with 10% paraformaldehyde in PBS for 10 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked and permeabilized in a 10% BSA solution with Triton- X (Thermo Fisher Scientific, Rockford, IL, USA). Subsequently ...
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bioRxiv - Bioengineering 2022Quote: Samples were denatured and reduced using 5× Lane Marker Reducing Sample Buffer (Thermo Scientific, Rockford, IL, US), boiled at 95°C for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Heat denatured protein samples were separated using a 4–12% NuPAGE gel (Thermo Scientific, Rockford, IL, USA). Proteins were transferred to PVDF membranes at 30 volts for 90 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Eight drops of the suspension (2 µl) were placed on Polysine slides (Thermo Scientific/Pierce, Rockford, IL), dried ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentration was measured with the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, Rockford, IL, USA); DNA integrity was checked by gel electrophoresis.
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bioRxiv - Physiology 2022Quote: ... and then stained for markers at 5 μg/ml for IL-17A-FITC (Thermofisher, 11-7177-81) and IFNg-PE (Thermofisher ...
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bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and serum IL-6 and TNF-α levels were measured using a commercial ELISA kit (Thermo Fisher). Serum CD5L levels were measured using ELISA ...
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bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization for 3 min with 0.15% Surfact-Amps X-100 (28314, Thermofisher Scientific, Rockford, IL) in PBS ...
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... IL-4 and IFN-ᵧ in supernatant was measured according to the manufacturer’s protocol (all Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... following chemiluminescent detection (SuperSignal® West Pico PLUS and West Femto Chemiluminescent Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Cancer Biology 2023Quote: ... ANBL-6 cells were also supplemented with 5 ng/ml IL-6 (Thermo Fisher Scientific, Cat# 206IL010).
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bioRxiv - Physiology 2023Quote: ... membrane was washed and developed using a SuperSignalTM west pico plus chemiluminescent substrate (Thermo Scientific, IL, USA). The blot was developed in iBright 1500 imaging system (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA following manufacturer’s instructions (Thermo Fisher Scientific #88808688).
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bioRxiv - Neuroscience 2023Quote: ... Some of the blots were then stripped (Restore PLUS Western Blot Stripping Buffer; Thermo Scientific, Rockford, IL) and reblotted if needed.
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bioRxiv - Genomics 2023Quote: Quantitative measurement of water-soluble protein performed using Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) [26] ...
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bioRxiv - Neuroscience 2024Quote: ... cells were fixed and immunostained for Iba-1 and cleaved IL- 1β (1:100, PA5-105048, Invitrogen) or CD68 and cleaved caspase 1 (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... T-cell cultures were supplemented with 200 U/mL IL-2 (Cat # 200-02, Peprotech (Thermo Fisher), Cranbury ...
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bioRxiv - Developmental Biology 2019Quote: ... stained with fluorophore-labeled secondary antibodies (Alexa 488 Donkey anti-Mouse, Alexa 555 Donkey anti-Mouse, Alexa 647 Donkey anti-Mouse, Alexa 488 Goat anti-Rabbit; Invitrogen, Carlsbad, CA) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405, Invitrogen #A-31553), tryptophan hydroxylase 2 (primary ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488/555/647 anti-rabbit IgG, anti-mouse IgG1, anti-mouse IgG2a, anti-mouse IgG2b, or anti-goat IgG; 1:1,000, Invitrogen, Grand Island, NY, USA). Nuclei were counterstained with DAPI (#62248 ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-rabbit or -mouse Alexa 488 and anti-mouse or -rabbit Alexa 568 (Invitrogen) secondary antibodies were used at a 1:250 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Immunohistochemistry for mCherry (mouse, 1:1000, Takara; goat anti-mouse-alexa594, 1:500, Invitrogen) was used to delineate viral vector spread ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the indicated secondary antibodies: goat α-mouse 488 mouse (Invitrogen A11029), goat α-mouse 568 (Invitrogen A11004) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse GSCs and mouse NSCs were fixed with 1% formaldehyde (Thermo Fisher scientific, 28906) for 10 min and quenched with 0.125 M glycine for 5 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-mouse IgG-Alexa 633 1:200 (mouse, A21052, Thermofisher, 1:200).
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bioRxiv - Bioengineering 2023Quote: ... an additional 45-min block was performed in mouse-on-mouse blocking reagent (Invitrogen). Sections were incubated with primary antibodies (Supplementary Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... and corresponding mouse mouse IgG1-PE isotype control (cat# 12-4714-42, ThermoFisher Scientific). Cells were washed twice in FW and analyzed by flow cytometry.
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bioRxiv - Physiology 2023Quote: ... A mouse anti-mouse antibody conjugated with Alexa Fluor 647 was used (Invitrogen A21058). 4′,6′-Diamidino-2-phenylindole staining was used to counterstain cellular nuclei ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-Actin (Invitrogen) at 1:3000 in 2% Bovine Serum Albumin (BSA) ...
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bioRxiv - Bioengineering 2020Quote: ... mouse anti-PGK1 monoclonal (Invitrogen)) at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-GAPDH (ThermoFisher AM4300), rabbit anti-Sra-1 (1/1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... or mouse IgG (ThermoFisher, 31903) were added to a spin column containing 20 ml of resin slurry in a 2-ml collection tube ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-V5 (Thermo Fisher Scientific Cat# R960-25 ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-mouse 680 (Invitrogen), goat anti-rabbit 800 (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... or mouse (Invitrogen, 61-6520) secondary antibody conjugated to horseradish peroxidase (diluted 1:50 000 in TBS-T+ 5% non-fat milk ...