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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and the protein concentration for each western blot sample was determined using BSA assays (Thermo Scientific, Rockford, IL, USA). Equal amounts of protein and varying amounts of sodium dodecyl sulfate buffer (SDS sample buffer ...
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bioRxiv - Immunology 2022Quote: Purified IL-2 expanded MAIT cells (MAITIL-2) were stimulated for 18 hours with anti-CD3/CD28 Dynabeads (Thermofisher), IL-12 (50ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Samples were stained using 50μl of intracellular anti-cytokine antibody stain: PE-Cy7-conjugated anti-IL-13 (eBio13A, Invitrogen), PE-conjugated anti-IL-5 (TRFK5 ...
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bioRxiv - Microbiology 2019Quote: ... The resulting peptides were subsequently labeled using a TMT-sixplex Isobaric Mass Tagging Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were assayed for IL-2 by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Thermo fisher). For flow cytometry ...
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bioRxiv - Bioengineering 2020Quote: ... AEX-HPLC analysis using Thermo Fisher Ultimate 3000 system with ProPacTM WAX-10 Columns (Thermo Scientific, Rockford, IL, USA) and RP-HPLC using Thermo Fisher Ultimate 3000 system with AdvanceBio RP-mAb Diphenyl Columns (Agilent Technologies).
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bioRxiv - Neuroscience 2020Quote: ... The protein levels in the supernatants were determined using the BCA Protein Assay Kit (Pierce/Thermo Scientific, Rockford, IL). To detect BDNF ...
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bioRxiv - Cancer Biology 2021Quote: ... Approximately 100ul of secondary antibodies diluted in 1% BSA+Triton-X were added (Thermo Fisher Scientific, Rockford, IL, USA). After incubation for 2.5 hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Nuclear extracts were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Kit (78833; Thermo Scientific, Rockford, IL, USA). The protein concentrations of both types of extracts were measured using the BCA Protein Assay Kit (23227 ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were lysed and inclusion bodies were purified using a B-Per protein purification kit (Thermo Scientific, Rockford, IL) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... the blots were developed using Supersignal west Pico plus Chemiluminescent substrate (Catalog No. 34580, Thermo Scientific, Rockfold, IL, USA) and then ...
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bioRxiv - Biochemistry 2021Quote: ... 5% NP-40% [Tergitol]) supplemented with 1X Halt protease and phosphatase inhibitor cocktail (from Thermo Scientific, Rockfold, IL, USA) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Cancer Biology 2020Quote: ... The expression of other genes was studied using the SYBR green method (Finnzymes F410L, Thermo Scientific, Rockford, IL, USA). The Ct values were normalized with ACTB ...
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bioRxiv - Immunology 2022Quote: ... Assay ID Mm99999915_g1), and IL-1R (Cat 4453320, Assay ID Mm00434237_m1) or with Mix SYBR Green Master Mix (Applied Biosystems).
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bioRxiv - Immunology 2019Quote: ... and IL-18 were determined using the Platinum ELISA kit for the quantitative detection of the three cytokines (ThermoFisher SCIENTIFIC Invitrogen ...
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bioRxiv - Immunology 2020Quote: The concentration of cytokines in the 24h and 48h supernatants from the T57 T cell activation assay was measured by ELISA according to the manufacturer’s instructions (IFNγ: Invitrogen eBioscience, cat#88731477, IL-2: Invitrogen eBioscience ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The liganded beads were blocked with excess biotin and washed with SEA BLOCK Blocking Buffer (Thermo Fisher, Rockford, IL), 1 % BSA ...
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bioRxiv - Immunology 2021Quote: ... major (WHO/MHOM/IL/80/Friedlin) parasites were grown in Schneider’s Drosophila medium (GIBCO BRL, Grand Island, NY, USA) supplemented with 20% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Physiology 2021Quote: ... Acidified samples were desalted in C18 desalting columns (Pierce Peptide Desalting Spin Columns, Thermo Fisher Scientific, Rockford, IL, USA) and reconstituted in 95% HPLC grade water ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice with 1X BD Perm/Wash Buffer (PWB) and stained with PE-eFluor610-IL-13 (Invitrogen), Brilliant Violet 421-IL-5 (Biolegend) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1x samples were run on a 1 % Agarose gel (BP1356-500, Fisher Bioreagent, Fisher Scientific, Hanover Park, IL, USA) in 1x TAE buffer at 110 V for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... with a 30 gauge by 0.5-inch needle (305106, BD Vacutainer Labware Medical, Fisher Scientific, Hanover Park, IL, USA). Each explant was transferred into a 1.5 mL microcentrifuge tube with snap cap with as little 1x PBS as possible ...
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bioRxiv - Cancer Biology 2021Quote: ... The CRISPR IL-32 KO vector was packaged into viral particles in HEK293T cells (Open Biosystems, Thermo Fisher Scientific), using second generation packaging plasmids psPAX2 ...
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bioRxiv - Microbiology 2020Quote: The remainder of the protein was incubated with 750 µl of HisPurTM Cobalt Resin (Thermo Scientific Pierce, Rockford, IL) on a rocker for 45 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... 48 h later cells were collected and RNA isolated with a Purelink RNA mini kit (ThermoFisher Sci., Rockford, IL) for RNA-Seq analysis as described32 ...
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bioRxiv - Immunology 2022Quote: ... and cytokine levels in the supernatant/PBS wash quantified using ELISA kits according to manufacturer’s instructions (murine IL-1β: Invitrogen 88-7013-22 ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed with PBS and ECL performed (SuperSignal West Pico, or SuperSignal West Femto, Thermo Fisher Scientific Inc, IL, USA).
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bioRxiv - Immunology 2022Quote: ... The resulting tryptic peptides were subsequently labelled using TMT-6plex Isobaric Mass Tagging Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s instructions as follows ...
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bioRxiv - Microbiology 2022Quote: ... Product sizes were evaluated using gel electrophoresis (1.5% agarose) with SYBR Safe DNA Gel Stain (Fisher Scientific, Chicago, IL) and purified using a Qiagen PCR Purification Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... The peptide samples were analysed on an LTQ-OrbitrapXL mass spectrometer Kit (Thermo Fisher Scientific, Rock ford, IL, USA) coupled to the Eksigent nanoLC-Ultra® 2D system (AB Sciex ...
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bioRxiv - Neuroscience 2022Quote: ... Punches were homogenized and assessed for protein concentration using a Pierce Bicinchoninic Acid (BCA) assay (Thermo Scientific, Rockford, IL) per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected for cytokine profiling by quantitative ELISA for IFNγ and IL-17A as per manufacturer’s instructions (Invitrogen). Prior to flow cytometric analysis for cytokine production ...
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bioRxiv - Cell Biology 2022Quote: ... cells (5×106/0.5 ml) were labeled with 100 μg Immunopure Sulfo-NHS-LC-Biotin (Thermo Scientific, Rockford, IL) for 30 min at 22 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantification of protein concentration was performed using the Pierce BCA Protein Assay Kit (23227, Thermo Fisher Scientific, IL, USA). For WB analysis ...
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bioRxiv - Cell Biology 2023Quote: ... rinsed with PBS and ECL performed (SuperSignal West Pico, or SuperSignal West Femto, Thermo Fisher Scientific Inc, IL, USA).
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... The bispecific antibody was purified from the collected supernatant using a protein A column (Thermo Scientific # 89960; IL, USA) and ELISA was used to assess ligand binding affinity and sensitivity of the antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... analysis was performed using TaqMan gene expression assay with ST2 and IL-1RAcP specific primers (Hs00249384_m1 and Hs00895050_m1 respectively, Life Technologies). Normalization of ST2 expression was performed using GADD45a (Hs00169255_m1 ...
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bioRxiv - Genetics 2023Quote: ... as well as editing the PAM site to protect against further edits) (Eurofins, Fisher Scientific LLC, Chicago, IL, USA). The gRNAs ...
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bioRxiv - Genomics 2023Quote: ... The LC-MS/MS system used consisted of an LTQ/Orbitrap-XL mass spectrometer (Thermo Scientific, Rockford, IL, USA) equipped with a Nanoacquity UPLC system (Waters ...
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bioRxiv - Plant Biology 2023Quote: ... Total protein content in the supernatant was estimated using the Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL). The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... following the manufacturer’s protocol (Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit; ThermoFisher Scientific, Rockford, IL, USA). From each sample ...
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bioRxiv - Microbiology 2023Quote: ... USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific, Rockford, IL, USA) at the Vienna Research Platform for Metabolomics & Proteomics and analyzed using the Proteome Discoverer v2.2.0.388 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.6) with 20 mg lysozyme and half of a crushed Pierce Protease Inhibitor Tab (Thermo Scientific, Rockford, IL) and frozen at −20°C ...
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bioRxiv - Pathology 2023Quote: ... fixed in 4% paraformaldehyde for 10 min and stained with ALP staining kit (Thermo Fisher Scientific, Rockford, IL, USA) for 15 min.
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bioRxiv - Microbiology 2023Quote: ... The supernatant was concentrated 500-fold using 100 kDA molecular weight cut-off filters (Thermo Scientific Pierce, Rockford, IL) that were centrifuged at 2,500 x g at 4°C until appropriately concentrated ...
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bioRxiv - Microbiology 2023Quote: ... 2 ug of EV protein content were separated on a 4-12% Bis-Tris gel (Thermo Scientific, Rockford, IL), then blotted onto a PVDF membrane ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Cell Biology 2023Quote: ... A stock doxorubicin-HCl solution was initially created by dissolving doxorubicin-HCl (Fisher Scientific, Hanover Park, IL, United States) in DMSO ...
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bioRxiv - Microbiology 2024Quote: ... We quantified the abundance of the peptides using the Pierce Micro BCA assay (Thermo Scientific Pierce, Rockford, IL, USA) following the manufacturer’s instructions.