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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Pierce Bicinchoninic acid (BCA) and MicroBCA protein assay kits were purchased from Thermo Scientific (Rockford, IL). Quant-iT PicoGreen dsDNA assay kit was procured from Molecular Probes ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were transferred onto Biodyne B nylon membranes (0.45 μm; Thermo Fisher Scientific, Rockford, IL, USA) in 0.5X TBE buffer (60V for 30 min) ...
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bioRxiv - Biochemistry 2021Quote: ... that contained 1mg/ml Texas Red-dextran (10,000 MW, Lysine Fixable, Thermo Fisher Scientific, Rockford, IL) at 37°C for 6-24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... M1 tri-Ub was purified to homogeneity by Ni2+-NTA affinity column (Thermo Scientific, Rockford, IL) chromatophraphy ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was determined using the bicinchoninic acid assay (BCA) (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was determined by the Bicinchoninic acid method (Pierce, ThermoFisher Scientific Fisher, Rockford, IL, USA). Aliquots of lysates containing 30 μg of protein were separated on SDS-PAGE and transferred to Immobilon-P membranes ...
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bioRxiv - Cell Biology 2021Quote: Samples were analyzed on Orbitrap Fusion Lumos Plus mass spectrometers (Thermo Fisher Scientific, Rockford, IL, USA) coupled with an Easy-nLC 1000 nanoflow LC system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with LIVE/DEADTM fixable Aqua dead cell stain (Thermo Fisher Scientific, Rockford, IL) for 30 min at 4oC ...
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bioRxiv - Biochemistry 2022Quote: ... Using Slide –A-Lyzer –Minidialysis units with a 10,000 Dalton MWCO(Thermo Scientific, Rockford, IL, USA) proteins were renatured by dialyzing out urea in a step wise manner from 6 M in steps of two at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... PKCα A (Cat# s11092) or PKCα B (Cat# s11094) silencer®select siRNA (Thermo Scientific, IL) using the lipofectamine RNAiMAX reagent as we described previously (24).
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bioRxiv - Neuroscience 2020Quote: ... RNA quality and concentration were measured using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Chicago, IL). 500-1000 ng RNA per sample was reverse-transcribed using Applied Biosystems high-capacity reverse transcription kit (Applied Biosystems ...
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bioRxiv - Physiology 2021Quote: ... were purchased from Badrilla (Leeds, UK), while RyR2 (1:1000, #MA3-916) was purchased from Thermo Fisher (Rockland, IL, USA). The primary antibodies against p62 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... then protein was quantified using BCA protein assay kit (Thermo Scientific, Rockford IL, USA, Ref. 23227) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2019Quote: ... the peptides were labelled with biotin by using Sulfo-NHS-LC-Biotin (Thermo Scientific, IL, USA). The unreacted Sulfo-NHS-LC-Biotin was quenched by 1 M glycine solution ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Alexa Fluor 633-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Alexa Fluor 633-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... each protein pellet was digested with 2 µg sequencing grade trypsin (Thermo Scientific, Rockford, IL, USA) overnight at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... cells were seeded into Lab-Tek CC2 treated slide chambers (Nalgene Nunc International, Napeville, IL, USA); after washing with PBS cells were fixed with 4% paraformaldehyde/PBS for 10 min ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA (Thermo Fisher Scientific #88-8086-77). Alternatively ...
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bioRxiv - Immunology 2019Quote: ... the human IL-2 ELISA kit was used in accordance with manufacturer’s instructions (Thermo Fisher Scientific). Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... and soluble Mpro proteins were purified with an Ni-NTA resin (Thermo-Fisher Scientific, Rockford, IL) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned using CloneJET PCR Cloning Kit (K1231, Thermo Scientific, Fisher Scientific, Hanover Park, IL, USA). Full-length duck ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with Alexa Fluor 488-conjugated Phalloidin at 0.005 units/αl (Molecular Probes/Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with Alexa Fluor 488-conjugated Phalloidin at 0.005 units/αl (Molecular Probes/Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Immunology 2021Quote: ... TaqMan gene expression kits were utilized to measure target genes of interest: Il-1β (Mm00434228_m1, ThermoFisher), Il-6 (Mm00446190_m1 ...
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bioRxiv - Bioengineering 2022Quote: ... sera from individual mice were added to peanut coated Maxisorp Immunoplates (Nalge Nunc International, Naperville, IL). Peanut-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugated (Southern Biotechnology Associates ...
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bioRxiv - Bioengineering 2022Quote: ... sera from individual mice were added to peanut coated Maxisorp Immunoplates (Nalge Nunc International, Naperville, IL). Peanut-specific IgE Abs were detected with goat anti-mouse IgE-unlabeled (Southern Biotechnology Associates ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... the yeast genomic DNA was extracted with Yeast DNA Extraction Kit (Thermo Fisher, Rockford, IL, USA). Three primers ...
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bioRxiv - Microbiology 2022Quote: ... before it was developed with SuperSignal West Dura Extended Duration substrate (Thermo Fisher Scientific, IL, USA). The blot was imaged using a Bio-Rad Chemidoc XRS+ (Bio-Rad ...
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bioRxiv - Systems Biology 2022Quote: ... The cell lysates were prepared in RIPA buffer containing protease inhibitors (Thermo Fisher Scientific, Rockford, IL) immediately after treatment ...
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bioRxiv - Neuroscience 2022Quote: ... NPC cultures received 100 pM acetic acid vehicle or 100 ng/ml IL-6 (Gibco; PHC0066) on day 18 for 3h ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were used to perform ELISA to quantify the levels of IL-1β (Thermo Fisher Scientific), IL-6 (Biolegend) ...
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bioRxiv - Genetics 2022Quote: ... cDNA was amplified using High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems, Rockford, IL, USA). The GCLC sequence was amplified using the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were harvested for IL-1β measurement using a specific ELISA (Thermo Fisher, 88-7621-22). Apoptotic cells were generated by retroviral transduction or MTX treatment during 20-24 h when cell death approaches its maximum ...
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bioRxiv - Microbiology 2024Quote: ... Formulations were dialyzed against PBS (pH 7.2) in the dialysis cassettes (Thermo Scientific, Rockford, IL, USA) for at least 18 h ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). The reaction conditions were as follows ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). Amplification was accomplished over 30 cycles as follows ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). The reaction conditions were as follows ...
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bioRxiv - Immunology 2024Quote: ... The cells were treated for 18-24 hours with 10 ng/mL IL-1β (Gibco, #PHC0815) and 5 ng/mL TNF (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... and activated 1:1 with CD3/CD28 Dynabeads in 100 U/mL of IL-2 (Thermofisher). The next day ...
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bioRxiv - Immunology 2023Quote: ... and IL-12 was quantified with ELISA kits according to the manufacturer’s instructions (Invitrogen, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Serum was run through an interleukin-6 (IL-6) enzyme-linked immunoabsorbent assay (ELISA) kit (Invitrogen). Successful maternal immune activation in offspring of Poly(I:C ...
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bioRxiv - Molecular Biology 2023Quote: ... or inserts in 24-well plates (0.4 μm polyester membrane, CoStar, Thermo Fisher Scientific, Rockford, IL) until 90% confluence was achieved(102) ...
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bioRxiv - Biophysics 2023Quote: ... and then immunoreactive bands were visualized using Super Signal WestPico chemiluminescent substrate (Thermo Scientific, Rockford, IL). Densitometric analysis of nonsaturated films was performed using Bio-Rad Quantity One analysis software (Hercules ...
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bioRxiv - Biochemistry 2023Quote: ... Nuclear fractions were obtained using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, IL, USA).
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bioRxiv - Immunology 2023Quote: ... LUVA supernatants were assayed for IL-6 and TNF (Invitrogen, #88-7066-88, #88-7346-88). β-hexosaminidase was measured in fresh supernatants by transferring 20µl sample/well to a 96 well plate (Sarstedt ...
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bioRxiv - Neuroscience 2023Quote: ... Detection was performed through the chemiluminescence assay with an ECL plus kit (Thermo Scientific, IL, USA). Densitometric detection was carried out with the LI-COR Odyssey imager and Image Studio software (version 5.2 ...
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bioRxiv - Cell Biology 2023Quote: ... and the resultant tryptic peptides were labeled with acetonitrile-dissolved TMT reagents (Thermo Scientific, Rockford, IL), and equal amounts of labeled samples were mixed before prefractionation with reversed phase (RP)-high performance liquid chromatography (HPLC) ...