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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Sandwich ELISA was used to establish plasma concentrations of IL-18 (Invitrogen, Thermofisher, BMS618-3) and ferritin (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... The ligation products (5 μl) were transformed into DH10B Competent Cells (Thermo Scientific, Rockford, IL) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ZO-1 (mouse monoclonal, Invitrogen 33-9100, 1:500), rabbit anti-aPKC (rabbit polyclonal ...
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bioRxiv - Immunology 2020Quote: ... mouse IFNβ (PBL Biomedical Laboratories) and mouse CXCL10 ELISA (ThermoFisher; BMS6018MST) were obtained from commercial sources.
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bioRxiv - Developmental Biology 2023Quote: ... Mouse aggregation medium (mouse AM) consisted of 1x DMEM (GIBCO-41965) supplemented with 20% FBS (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-©tubulin (mouse monoclonal, MilliporeSigma T6557, 1:200) and mouse anti-ZO-1 (Life Technologies, 1:300). Rabbit anti-Pard3 (rabbit polyclonal ...
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bioRxiv - Cell Biology 2020Quote: The frozen supernatants were used for IL-2 ELISAs according to the manufacturer’s protocol (KMC0021, Thermofisher).
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bioRxiv - Immunology 2021Quote: ... containing the cytokines IL-4 (500 U/mL) and GM-CSF (800 U/mL) (both Gibco) for differentiation into DCs ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific, Rockford, IL, USA). Samples were prepared for SDS-PAGE in Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from the heart using TRIzol reagent (Thermo Fisher Scientific, Rockford, IL, USA) as reported previously.8 Total RNA was reverse transcribed into cDNA using random primers (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the Qubit® Protein Assay Kit (Thermo Scientific Rockford, IL, USA). For both cases ...
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bioRxiv - Neuroscience 2021Quote: ... The protein band was detected using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2021Quote: The LysoTracker Red assay was used to follow the manufacturer’s protocol (LifeTechnology, ThermoFisher, Rockford, IL, USA). LysoTracker Red (50 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% sodium dodecyl sulfate (SDS) in 50 mM triethylammonium bicarbonate (TEAB, Thermo Fisher Scientific, Rockford, IL), pH=8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were prepared in the modified RIPA buffer containing protease inhibitors (Thermo Scientific, Rockford, IL). Equal amounts of proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was estimated by BCA (Pierce BCA Protein Assay Kit, Thermo Scientific, Rockford, IL, USA). Denatured proteins were reduced with 5 mM DTT ...
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bioRxiv - Biochemistry 2019Quote: Primary enriched CM and hiPSC-CM were lysed in 1X Invitrosol (Thermo Fisher Scientific, IL, USA), 100 mM ammonium bicarbonate (NH4HCO3) ...
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bioRxiv - Immunology 2019Quote: ... The protein concentrations were determined by the bicinchoninic acid assay (BCA, Pierce Biotechnology, Thermo Scientific, IL). Further processing was adapted from a filter-aided sample preparation (FASP ...
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bioRxiv - Physiology 2020Quote: ... The PCR amplifications were performed with SYBR green qPCR master mix (#K0252, Thermo Scientific, Rockford, IL). Total RNA was prepared from the frozen whole uterine and placental tissues ...
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bioRxiv - Biophysics 2019Quote: ... proteins were purified using HisPur Cobalt Resin according to the manufacturer’s protocol (Thermo Scientific, Rockford, IL.) and Ulp-1 protease (Ubl-specific protease 1 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher scientific Inc., Rockford, IL). CHT-mediated choline uptake was determined as total choline uptake minus the uptake in the presence of hemicholinium-3 and maximum transporter velocity (Vmax ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected and used for cytokine measurement with IL-18 (Invitrogen; sensitivity 19.0 pg/mL), IL-1β (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: Pierce Bicinchoninic acid (BCA) and MicroBCA protein assay kits were purchased from Thermo Scientific (Rockford, IL). Quant-iT PicoGreen dsDNA assay kit was procured from Molecular Probes ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were transferred onto Biodyne B nylon membranes (0.45 μm; Thermo Fisher Scientific, Rockford, IL, USA) in 0.5X TBE buffer (60V for 30 min) ...
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bioRxiv - Biochemistry 2021Quote: ... that contained 1mg/ml Texas Red-dextran (10,000 MW, Lysine Fixable, Thermo Fisher Scientific, Rockford, IL) at 37°C for 6-24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... M1 tri-Ub was purified to homogeneity by Ni2+-NTA affinity column (Thermo Scientific, Rockford, IL) chromatophraphy ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was determined using the bicinchoninic acid assay (BCA) (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was determined by the Bicinchoninic acid method (Pierce, ThermoFisher Scientific Fisher, Rockford, IL, USA). Aliquots of lysates containing 30 μg of protein were separated on SDS-PAGE and transferred to Immobilon-P membranes ...
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bioRxiv - Cell Biology 2021Quote: Samples were analyzed on Orbitrap Fusion Lumos Plus mass spectrometers (Thermo Fisher Scientific, Rockford, IL, USA) coupled with an Easy-nLC 1000 nanoflow LC system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with LIVE/DEADTM fixable Aqua dead cell stain (Thermo Fisher Scientific, Rockford, IL) for 30 min at 4oC ...
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bioRxiv - Biochemistry 2022Quote: ... Using Slide –A-Lyzer –Minidialysis units with a 10,000 Dalton MWCO(Thermo Scientific, Rockford, IL, USA) proteins were renatured by dialyzing out urea in a step wise manner from 6 M in steps of two at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... PKCα A (Cat# s11092) or PKCα B (Cat# s11094) silencer®select siRNA (Thermo Scientific, IL) using the lipofectamine RNAiMAX reagent as we described previously (24).
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bioRxiv - Neuroscience 2020Quote: ... RNA quality and concentration were measured using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Chicago, IL). 500-1000 ng RNA per sample was reverse-transcribed using Applied Biosystems high-capacity reverse transcription kit (Applied Biosystems ...
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bioRxiv - Physiology 2021Quote: ... were purchased from Badrilla (Leeds, UK), while RyR2 (1:1000, #MA3-916) was purchased from Thermo Fisher (Rockland, IL, USA). The primary antibodies against p62 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... then protein was quantified using BCA protein assay kit (Thermo Scientific, Rockford IL, USA, Ref. 23227) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2019Quote: ... the peptides were labelled with biotin by using Sulfo-NHS-LC-Biotin (Thermo Scientific, IL, USA). The unreacted Sulfo-NHS-LC-Biotin was quenched by 1 M glycine solution ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Alexa Fluor 633-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Alexa Fluor 633-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... each protein pellet was digested with 2 µg sequencing grade trypsin (Thermo Scientific, Rockford, IL, USA) overnight at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... cells were seeded into Lab-Tek CC2 treated slide chambers (Nalgene Nunc International, Napeville, IL, USA); after washing with PBS cells were fixed with 4% paraformaldehyde/PBS for 10 min ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA (Thermo Fisher Scientific #88-8086-77). Alternatively ...
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bioRxiv - Immunology 2019Quote: ... the human IL-2 ELISA kit was used in accordance with manufacturer’s instructions (Thermo Fisher Scientific). Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... and soluble Mpro proteins were purified with an Ni-NTA resin (Thermo-Fisher Scientific, Rockford, IL) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned using CloneJET PCR Cloning Kit (K1231, Thermo Scientific, Fisher Scientific, Hanover Park, IL, USA). Full-length duck ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with Alexa Fluor 488-conjugated Phalloidin at 0.005 units/αl (Molecular Probes/Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with Alexa Fluor 488-conjugated Phalloidin at 0.005 units/αl (Molecular Probes/Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Immunology 2021Quote: ... TaqMan gene expression kits were utilized to measure target genes of interest: Il-1β (Mm00434228_m1, ThermoFisher), Il-6 (Mm00446190_m1 ...
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bioRxiv - Bioengineering 2022Quote: ... sera from individual mice were added to peanut coated Maxisorp Immunoplates (Nalge Nunc International, Naperville, IL). Peanut-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugated (Southern Biotechnology Associates ...
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bioRxiv - Bioengineering 2022Quote: ... sera from individual mice were added to peanut coated Maxisorp Immunoplates (Nalge Nunc International, Naperville, IL). Peanut-specific IgE Abs were detected with goat anti-mouse IgE-unlabeled (Southern Biotechnology Associates ...