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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature for all target proteins or with clear milk blocking buffer for β-actin protein (Pierce, Thermo Scientific). We used the following LARP1 (Bethyl Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated onto Geltrex-coated dishes with MEF conditioned medium (CM) (DMEM/F12 supplemented with 15% KSR, 100μM β-mercaptoethanol, 1% nonessential amino acid stock (all from Thermo Fisher Scientific), 10ng/mL bFGF and 10 mM Y-27632 (both from the Institute for Technical Chemistry ...
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bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2021Quote: ... WT PA28γ and PA28γ2XCys were incubated in 0.1% β-Mercaptoethanol for 1 hour before being desalted using a Zeba™ Spin Desalting Column (Thermo Scientific). Proteins were then incubated at 37°C for 10 minutes in 0.1% β-Mercaptoethanol or Tetrathionate (1mM ...
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bioRxiv - Biophysics 2021Quote: ... Cells were induced with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 hours and harvested by centrifugation (Sorvall LYNX 6000, ThermoFisher Scientific) at 8,000 rpm for 10 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... Clone cultures were concentrated to 20-fold in M9 buffer and 10 μL was plated onto a well of a 96 well plate containing NGM agar with 2 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG, Fisher Scientific). Plates were dried and stored at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Microbiology 2022Quote: Antibodies used for immunofluorescence and flow cytometry include: mouse anti-β subunit of the ATP synthase (1:500 dilution; Invitrogen, A21-351); rabbit anti-BNIP3L/NIX (1:100 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended at a concentration of 1×104 cells per mL in complete RPMI 1640 cell culture media (10% FBS, 1% P/S) with 50 μM β-mercaptoethanol (Gibco 21985023). 100 μL of this cell suspension was added to each well such that each tumoroid initially started with approximately 1×103 cells ...
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bioRxiv - Neuroscience 2023Quote: ... Immunocomplexes were isolated with protein A/G beads (Thermo, 20421) and eluted 1× LDS sample buffer with 10% β-mercaptoethanol (Invitrogen; NP0007) at 55 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated on ice for 15 min and protein expression was initiated with the addition of 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Fisher Scientific; BP1755). Cells were then incubated for 16–18 hr at 18°C for optimal protein production.
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bioRxiv - Biochemistry 2024Quote: ... HBMEC were incubated overnight at 4°C with the primary antibodies (anti-β-catenin, # 71-2700; anti-ZO-1, # 61-7300; anti-CAT1, # PA5-82800, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended at a concentration of 1x104 cells per mL in complete RPMI 1640 cell culture media (10% FBS, 1% P/S) with 50 μM β-mercaptoethanol (Gibco 21985023). 100 μL of this cell suspension was added to each well such that each tumoroid initially started with approximately 1x103 cells ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were incubated with a 1:200 dilution of human cTnT primary mouse antibody (ThermoFisher) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Microbiology 2020Quote: The human acute leukemia monocyte cell line (THP-1) was cultivated in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated FBS (Thermo Scientific Hyclone ...
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bioRxiv - Biochemistry 2022Quote: ... The ORF of human MYBL2/B-Myb isoform 1 (NM_002466.4) was cloned into pcDNA3.1+ (ThermoFisher Scientific) and fused with an N-terminal Flag tag ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse-anti-human H3K9/14/18/23/27ac (Thermo Fisher Scientific, Carlsbad, CA, USA; 1:500) was used to detect this histone modification in HPdLF and goat-anti-Mouse-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2021Quote: ... Alexa488- or Rhodamine-conjugated goat anti-human secondary antibody was added (1/500 dilution) (Thermo Fisher), and incubated for 2~3 hr at room temperature in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... Immortalized human fibroblasts were cultured in DMEM (sigma) supplemented with 1% of penicillin and streptomycin (Gibco) and 10% fetal bovine serum (Corning ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with goat polyclonal anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... plates were incubated with a goat anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific). For IgG subclasses and IgM detection ...
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bioRxiv - Immunology 2021Quote: Human THP-1 cells (ATCC® TIB-202™) were cultured in RPMI media (22400089, ThermoFisher) supplemented with 1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:3000 in blocking buffer or HRP-labelled goat anti-human IgM (Thermo Fisher Scientific) diluted 1:4000 at 37°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and 1:5,000 diluted goat anti-human-IgG Fc horseradish peroxidase (HRP) conjugated secondary antibodies (Invitrogen) were added ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ng/mL human basic FGF2 (Miltenyi Biotech, 130-093-840) and kanamycin (Gibco, 15160-054). Culture medium was exchanged every 2 days ...
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bioRxiv - Bioengineering 2023Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-human IgM and IgA (Invitrogen, A18835 and A18781, 1:5,000), rabbit anti-human IgG antibody (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: DNA encoding the ECR of human ADGRB2/BAI2 (aa 1-921) was synthesized by Thermo Fisher GeneArt ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-Human IgG (H+L) Alexa Fluor 488 conjugated (Invitrogen, A-11013, dilution 1:500); Donkey Anti-Mouse IgG H&L Alexa Fluor 647 conjugated (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-Human IgG (H+L) Alexa Fluor 488 conjugated (Invitrogen, A-11013, dilution 1:500). Data acquisition was performed with FACSVerse™ (BD ...
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bioRxiv - Neuroscience 2024Quote: ... the mean fluorescence intensity (MFI) of Alexa488-coupled goat anti-human IgG (1:500; Life Technologies) was evaluated ...
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bioRxiv - Cancer Biology 2023Quote: 1 × 105 OCSC1-F2 human OC cells were resuspended in serum-free Opti-MEM (Thermo Scientific) and seeded on trans-well inserts with a polyethylene terephthalate membrane pore size of 8.0 μm (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies) enabled in vitro translation of these constructs ...
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bioRxiv - Genomics 2020Quote: ... Ligation reactions were transformed into One Shot™ Stbl3™ chemically-competent E.coli cells (42°C heat-shock, 90 seconds; Invitrogen C737303) and positive clones were selected by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were differentiated for the following 26 days (42 days of total differentiation) in neuronal differentiation media consisting of Neurobasal Media (Thermo Fisher Scientific) + B27 supplement (without Vitamin A ...
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bioRxiv - Genomics 2021Quote: ... The suspension was filtered through a 42-μm nylon mesh and stained with the intercalating fluorochrome propidium iodide (PI, Thermo Fisher Scientific) and treated with RNase II A (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... This reaction was incubated for 60 minutes in triplicates for each temperature (37°C, 40°C and 42°C) and terminated by adding one volume of sample buffer (NuPAGE LDS; Invitrogen, Carlsbad, CA) containing 5% of 2-β-mercapto-ethanol ...
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bioRxiv - Genomics 2021Quote: ... The suspension was filtered through a 42-μm nylon mesh and stained with the intercalating fluorochrome propidium iodide (PI, Thermo Fisher Scientific) and treated with RNase II A (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Absence of lipopolysaccharide (LPS) (< 20EU/ml (42)) in purified CPS was confirmed by Pierce™ LAL Chromogenic Endotoxin Quantitation Kit (Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... Extracted DNA was further purified using the ethanol precipitation protocol.42 The quantity and quality of the resulting DNA extractants were evaluated using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific) as well as by gel electrophoresis.43 All the DNA samples were stored at -20 °C before further processing.
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bioRxiv - Bioengineering 2023Quote: ... extension: 68 °C for 42 secs and total number of cycles: 35) in ProFlex 3×32-well PCR system (Applied Biosystems, USA). Desired amplified product was excised from 1.2% agarose gel and purified using GeneJET gel extraction and DNA cleanup kit following supplier’s protocol (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... carinii+Rattus norvegicus or P.murina+Mus musculus UniProt database (42; accessed 06/2020) using Proteome discoverer v2.4 and the Sequest HT search algorithm (Thermo Scientific, Waltham, MA). Proteomic analysis was performed on 3 independent EV isolations and data was concatenated for coverage of the proteome ...
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bioRxiv - Biophysics 2023Quote: In vitro cytotoxicity of the complexes was evaluated using U-2 OS cells and an LDH assay kit.42 Cells were counted with a Countess II Automated Cell Counter (ThermoFisher Scientific, USA) and seeded in 96-well plates at a density of 10,000 cells per well in 100 μL of full DMEM ...
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bioRxiv - Plant Biology 2023Quote: ... L−1 of 3’-biotin-labeled primer probe purified by high-pressure liquid chromatography (HPLC) for 4-12 h at 42°C in Hybaid Shake ‘n’ Stack (Thermo Fisher Scientific) with gentle rotation ...
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bioRxiv - Microbiology 2023Quote: Cells were grown in 16×25 mm glass tubes with 3 ml of Hv-Cab (de Silva et al., 2021) at 42°C under constant agitation with the inducers D-xylose (Thermo Scientific Chemicals), L-arabinose (Thermo Scientific Chemicals) ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of Aβ1-40 and Aβ1-42 in the s-extracts was determined by commercially available ELISA kits (ThermoFisher Scientific: KHB3481 and KHB3544). Aβ1-42 was undetectable in the Aβ negative sample ...
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bioRxiv - Microbiology 2020Quote: ... ExsE and SipC was visualized using β-lactamase (MA120370, Fisher Scientific) (7.5% gel) ...
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bioRxiv - Genetics 2019Quote: ... The primary antibodies used were anti-β-actin (ThermoFisher #MA5-15739) used at a 1:10,000 dilution and anti- ΔN-p73 (ThermoFisher #MA5-16183 ...
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bioRxiv - Bioengineering 2021Quote: ... and isopropyl-β-D-galactopyranoside (IPTG) were purchased from Thermo Fisher Scientific ...