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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... anti-CD9 (1:500, mouse monoclonal anti-human CD9, clone MM2/57, Invitrogen, AHS0902). The membranes were then washed three times with 0.1% Tween 20 in PBS for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: Human primary fibroblasts FIB-1 and FIB-2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS and Pen-Strep antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 633-conjugated goat anti-human antibody (1:500; A21091 from Life Technologies); Alexa Fluor 555-conjugated goat anti-rabbit (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... Viable human tumor tissue pieces of ∼1-2mm3 were thawed in prewarmed DMEM (Gibco) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2019Quote: The human colorectal cancer cell line DLD-1 was cultured in DMEM (Life Technologies) supplemented with heat-inactivated 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Systems Biology 2020Quote: ... The human monocytic cell line THP-1 was maintained in RPMI 1640 medium (Gibco). All media were supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... and anti-human AlexaFluor 647 (1:500, 30 minutes, 4°C; Life Technologies, A21445), and resuspended in CellFix (1:10 in water ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and secondary anti-human IgG antibodies (1:2000) conjugated to Alexa fluor 488 (Invitrogen). Cells staining positive for Spike were measured by flow cytometry on a Becton Dickinson BD LSR II Special Order System Flow Cytometer With HTS Sampler ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 647–conjugated goat anti-human (1:1000; A-21445; Thermo Fisher Scientific). Secondary antibodies were dilute in PBST and incubated for 30 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa fluor 488 Goat anti-Human IgG Secondary (#A-11013, dilution 1:100, Invitrogen); APC anti-human CD4 Antibody (#357408 ...
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bioRxiv - Microbiology 2023Quote: ... AlexaFluor 647-conjugated anti-human and AlexaFluor 594-conjugated anti-mouse (Invitrogen, 1:1,000) antibodies ...
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bioRxiv - Immunology 2022Quote: ... incubated with a 1:10,000 dilution goat anti-human IgG (H+L)-HRP (Invitrogen), and washed twice more before detection with ECL (ECL Plus Western Blotting Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Neuroscience 2023Quote: ... and an Alexa Fluor 488- conjugated goat anti-human secondary antibody (1:200, Invitrogen) were then used to reveal the immunopositive structure ...
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bioRxiv - Genomics 2023Quote: A serial dilution from 1 ng to 1,000 ng Human Liver Total RNA (ThermoFisher) was prepared to generate the various input samples ...
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bioRxiv - Biophysics 2023Quote: The gene encoding human A2AAR (1-316) was cloned into a pPIC9K vector (Invitrogen) at the BamHI and NotI restriction sites ...
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bioRxiv - Immunology 2024Quote: ... and anti-human IgG Alexa Fluor™ 647 (ThermoFisher, Cat. #A-21445, 1:3000) for 10 min at room temperature in dark ...
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bioRxiv - Microbiology 2024Quote: ... Human THP-1 monocytes obtained from ATCC were grown in RPMI 1640 medium (Gibco) containing 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... T lymphocytes were purified using the Pan T cell isolation kit (Miltenyi Biotech) and activated with Dynabeads Human T-Activator CD3/CD28 (1:1 beads:cell) (ThermoFisher) in X-vivo 15 medium (Lonza ...
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bioRxiv - Immunology 2022Quote: Supernatants were collected 3 days post Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco) stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel ...
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bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... slides were incubated for 1 hr with goat anti-human 555 (Thermo Fisher Scientific, A-21433; 1:1000 dilution) and goat anti-rabbit 488 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and for human biopsies (see Fig. S1B and C) goat anti-ZO-1 (Thermo Fisher; PA5-19090; 1:200) and anti-laminin primary antibodies were used ...
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bioRxiv - Immunology 2023Quote: ... primary human CD4 T cells were activated with anti-CD3+ anti-CD28 Dynabeads (1:1) (Thermofisher scientific, cat # 11161D) + IL-2 overnight and then 1 million aliquots of cells (triplicates ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed with T-cell medium (TCM, RPMI supplemented with 10% FBS [Welgene], 1% penicillin/streptomycin [Gibco], and 0.1% β-mercaptoethanol [Gibco]) and incubated with biotin-conjugated anti-CD3 (60 μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... the cells were washed once with PBS and lysed with 350 μl RLT buffer with 1% β-mercaptoethanol (Thermo Fisher). The RNA was then extracted using the RNeasy mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.075% (v/v) β-mercaptoethanol and 1 mM benzamidine] supplemented with one tablet of Pierce protease inhibitor cocktail (ThermoFisher Scientific) and lysed by sonication using a Sonics Vibracell instrument (1 s ON/3 s OFF ...
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bioRxiv - Microbiology 2023Quote: ... Bones were washed with BMDM media (20% HyClone FBS, 1% sodium pyruvate, 0.1% β-mercaptoethanol, 10% conditioned supernatant from 3T3 fibroblasts, in Gibco DMEM containing glucose and 100 U/ml penicillin and 100 ug/ml streptomycin ...
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bioRxiv - Bioengineering 2023Quote: INS-1 β-cells treated with respective conditions were stained with 250 nM MitoTracker Deep Red (Thermofisher scientific, Cat# M22426) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Bones were washed with BMDM media (20% FBS, 1% sodium pyruvate, 0.1% β-mercaptoethanol, 10% conditioned supernatant from 3T3 fibroblasts, in Gibco DMEM containing glucose and 100 U/ml penicillin and 100 ug/ml streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Blockade of β-actin: differentiated neurons and primary neurons were treated with 2µg of either anti β-actin (mouse IgG1) Antibody (Thermo Fisher Scientific), or normal mouse IgG1(Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and assayed for β-galactosidase assay using a Gal-Screen β-galactosidase reporter gene assay system for yeast or mammalian cells (Applied Biosystems) as per the manufacturer’s instructions and read with SpectraMax i3 (Molecular Devices) ...
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bioRxiv - Genetics 2022Quote: ... we established MDA-MB-231 cells with overexpressing β-catenin by transiently transfecting β-catenin/flag plasmid approximately 2 × 107 cells were lysed by using IP lysis buffer (Invitrogen, 87787), and obtained lysates were incubated with Flag antibody and Dynabeads™ protein G (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells are co-transfected with GPR1-WT-smBiT or CMKLR1-WT-smBiT (400 ng/well) and LgBiT-β-Arr1 or LgBiT-β-Arr2 (200 ng/well) by Lipofectamine™ 3000 (Invitrogen) for 24 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... was codon-optimized and cloned into a pFastBac dual vector(42) for production of baculovirus by the Bac-to-Bac method (Invitrogen). High Five Cells (Thermofisher ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by a digestion with the Nuclease and Enhancer proteins at 42°C before being electrophoresed on a 10% TBE Gel (Thermofisher). The gel was then stained in 100 ml 1x TBE buffer with 2 μl of 10 mg/ml ethidium bromide for 5 min ...
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bioRxiv - Genetics 2019Quote: ... anti-CD127-FITC and (30 µl/ml, clone eBioRDR5, Cat. no.11-1278-42, Thermo Fisher Scientific, Waltham, U. S.) and anti-CD25-PE (80 µl/ml ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from 3 × 106 Xela DS2 (passages 28, 29 and 31) and Xela VS2 (passages 42, 46 and 48) using TRI reagent (Invitrogen) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Immunology 2020Quote: ... and anti-CD127-PE-Cy5 (clone eBioRDR5; 5 μL; cat. # 15-1278-42) all from Thermo Fisher (Supplementary Fig. 4). mAbs for chemokine receptors (i.e ...
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bioRxiv - Cell Biology 2021Quote: ... were transformed into DH10EmBacY chemically competent cells with heat shock at 42°C for 15 seconds followed by incubation at 37°C for 5 hours in S.O.C media (Thermo Fisher Scientific). The cells were then plated on LB-agar plates containing kanamycin (50 μg/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42℃ using SuperScript II (Invitrogen, 18064-014 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated overnight at 42°C with probe in hybridization buffer (50% formamide, 5X SSCPE, 5X Denhardt’s solution (Invitrogen 750018), 0.1 mg/ml salmon sperm DNA (Agilent 201190) ...
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bioRxiv - Plant Biology 2021Quote: ... Released RNA from permeabilized tissues was captured by the DNB and reverse transcribed overnight at 42 °C using SuperScript II (Invitrogen, 18064-014 ...