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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Human β Amyloid 1 42 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The membrane was crosslinked using a UV stratalinker and blocked at 42°C using ULTRAhyb Oligo hybridization and blocking buffer (Ambion) for 2 hours while rotating ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were hybridized at 42 °C with 5 nM of a 5’-biotinylated oligonucleotide probe (Table S4) in ULTRAhyb Ultrasensitive Hybridization Buffer (Ambion) and then washed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... was carried out overnight (∼18-20h) at 42°C by incubating permeabilized tissue with 75 µl cDNA synthesis mix containing 1X First strand buffer (Invitrogen), 5 mM DTT ...
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bioRxiv - Immunology 2020Quote: Livers were harvested 40-42 hours after sporozoite challenge and total RNA was extracted following homogenisation using TRIzol (ThermoFisher Scientific). cDNA was generated using the RETROScript Kit (Ambion) ...
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bioRxiv - Cancer Biology 2020Quote: ... was carried overnight (∼18-20h) at 42°C by incubating permeabilized tissue with 75 µl cDNA synthesis mix containing 1X First strand buffer (Invitrogen), 5 mM DTT ...
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bioRxiv - Neuroscience 2019Quote: ... RAI1 antibodies were generated by immunizing rabbits with a synthesized RAI1 peptide (aa 28 to 42, ENYRQPGQAGLSCDR, Thermo Fisher Scientific), followed by affinity purification using the peptide as the affinity ligand (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using 1 ug of RNA reverse transcribed for 50 min at 42°C using oligo(dT) primer (IDT) and SuperScript II RT (Invitrogen). For qPCR analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... into newly synthesized proteins 42 using the Click-it Plus OPP Alexa Fluor 647 Proteinsynthesis-Assay-Kit according to the manufacturer’s manual (Thermo Fisher). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... Applying currents in the 0-1000 mA range allowed the application of forces spanning from 0 to 42 pN using Dynabead M270 streptavidin-coated superparamagnetic beads (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 2 h at 42 °C in hybridization buffer (ULTRAhyb™ Ultrasensitive Hybridization Buffer [Invitrogen; AM8669]). The probe was added to hybridization buffer and hybridized to the blot overnight at 38 °C ...
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bioRxiv - Neuroscience 2023Quote: hiPSC-derived neurons after 38-42 days of terminal differentiation were transfected with pCAG-MaxGFP (GFP) using Lipofectamine 2000 (Invitrogen). The GFP-labeled neurons were imaged at 10x magnification using a Nikon C2 confocal fluorescence microscope ...
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bioRxiv - Neuroscience 2023Quote: ... 60 minutes at 42°C followed by 5 minutes at 70°C or using the high-capacity cDNA reverse transcription kit (4368814, ThermoFisher) with the following programme ...
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bioRxiv - Molecular Biology 2023Quote: ... 32P-labelled oligonucleotides (listed in Table S4) were hybridized overnight at 42°C in ULTRAhyb®-Oligo hybridization buffer (Ambion). After hybridization ...
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bioRxiv - Molecular Biology 2023Quote: ... Stock probes were diluted 1:1000 in RNA FISH hybridization buffer preheated to 42°C (15% deionized formamide, 10% dextran sulfate, 2× SSC from Ambion, 0.02% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were stained for 30 min on ice with pre-optimized concentrations of the following fluorochrome-conjugated antibodies: AREG-APC (17-5370-42; Invitrogen), CD49f (ITGA6)-PE (561894/555736 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA released from the permeabilized tissue and captured by the DNB was reverse transcribed overnight at 42°C using SuperScript II (Invitrogen, 18064-014 ...
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bioRxiv - Cancer Biology 2024Quote: ... Dnmt3L KO clones (QKO) were generated by transfecting TKO J1 mESC (42) with sgRNA-containing px458 using Lipofectamine 3000 (Invitrogen) and sorting GFP+ cells after 72h ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Developmental Biology 2021Quote: ... S2 cells were lysed to measure β-galactosidase activity using the Dual-Light Luciferase & β-Galactosidase Reporter Gene Assay System (T1003; Thermo Fisher Scientific). Luciferase activity was used for normalizing transfection efficiency.
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bioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with the following primary antibodies: rabbit anti-Profilin 1 (Novus Biologicals, NB200-262, 1:10’000 for human lysates; Invitrogen, PA5- 17444 ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated with a 1:10000 dilution (in PBST with 1% BSA) of goat anti-human Fc HRP-conjugated antibody (Invitrogen) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... immortalized human lung fibroblast cells and HCT116 colorectal cancer cells were cultured in a 1:1 mixture of DMEM (Gibco) and Ham’s F10 (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... Sorted Treg cells from HD and IPEX patients were stimulated with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human endogenous PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ cells were combined at a 1:1 ratio and activated using anti-human CD3/CD28 Dynabeads (Invitrogen, #40203D) at a 3:1 ratio ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Molecular Biology 2019Quote: The human keratinocytes N/TERT-1 cells (27) were cultured in keratinocyte SFM media (Thermofisher) at 37°C under 5% CO2 atmosphere ...
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bioRxiv - Immunology 2019Quote: ... and human/mouse TGF beta 1 ELISA Ready-SET-Go! Kit (2nd Generation; Affymetrix, eBioscience), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... was obtained from Abcam (Cat.# ab9498) (Cambridge, MA, USA) and anti-human ZO-1 antibody was obtained from Invitrogen / ThermoFisher (Carlsbad ...
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bioRxiv - Cell Biology 2021Quote: ... Bound B2-IgG was stained with goat anti-human IgG-Alexa 568 (1:1,000; Invitrogen) for 30 min and analyzed by FACS ...
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bioRxiv - Microbiology 2020Quote: Human DHFR isoform 1 cDNA was codon optimized and purchased as a gene block (Invitrogen) containing 5’ NdeI and 3’ BamHI cut sites ...
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bioRxiv - Microbiology 2020Quote: ... After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the cells were analyzed by flow cytometer (BD LSRFortessa™ ...
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bioRxiv - Cancer Biology 2021Quote: ... PerCP-eFluor 710 mouse anti-human CD223 (LAG-3) (1:100; 46-2239-41, Invitrogen), and BV421 mouse anti-human CD366 (TIM-3 ...
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bioRxiv - Biochemistry 2022Quote: ... Select Pre-designed siRNA for human cardiolipin synthase-1 (CLS) were obtained from Life Technologies Inc ...
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bioRxiv - Microbiology 2020Quote: THP-1 human monocytes (ATCC, TIB-202) were cultured in sterile RPMI-1640 medium (Gibco) supplemented with 1mM sodium pyruvate ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 μg/ml mouse anti-human anti-TRA-1-60 antibody (ThermoFisher Scientific, Waltham, MA), (3 ...
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bioRxiv - Neuroscience 2021Quote: ... and the secondary antibody Alexa Fluor® 633 goat anti-human IgG (1:2000, Invitrogen). Floating slices were rinsed 3×10 min with TBS and blocked with blocking buffer (10% NGS with 0.3% Triton X-100 in TBS ...
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bioRxiv - Neuroscience 2021Quote: The human iPSC lines iPSC-1 XMO01 (67) and iPSC-2 (A18945, Thermo Fisher Scientific) were cultured in standard conditions (37°C ...
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bioRxiv - Microbiology 2022Quote: ... 1:500 dilution) (goat anti-human IgG Fc Alexa 488 conjugated, Thermo Fisher, Cat #: H10120) (goat anti-human IgG (H + L ...
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bioRxiv - Immunology 2022Quote: ... mouse-anti-mouse/human/rat monoclonal PP1a (1:1000 dilution, number MA5-17239, ThermoFisher Scientific), rabbit-anti-Y.pestis polyclonal YopM (1:1000 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... at a dilution 1:2000 and rabbit anti-OCN antibody (Reactivity: human, Invitrogen, PA5-96529) at a dilution 1:100 in 1% BSA in PBS-T for 1h00 at room temperature ...
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived neurons (differentiation protocol 1) were harvested in TRIzol reagent (Invitrogen #15596026). Following TRIzol/chloroform extraction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed in PBS and incubated with anti-human Alexa-647 (1:500, ThermoFisher), and Hoechst 33342 (1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Alexa Fluor 488 goat anti-human IgG (H+L) (Invitrogen, cat # A11013, 1:1000).
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bioRxiv - Genetics 2022Quote: ... and stained with a monoclonal mouse anti-human HbF antibody (clone HBF-1, ThermoFisher scientific). Cells were analyzed by flow cytometry using a BD LSRFortessa cell analyzer (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Cell Biology 2023Quote: ... Human-Kinesin-1-EGFP-expressing Drosophila S2 cells were grown in Schneider’s medium (Life Technologies) supplemented with 10% heat inactivated fetal bovine serum (FBS ...