Labshake search
Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 8 Chloro 2 3 4 5 tetrahydropyrido 3 2 f 1 4 oxazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... The amplicon of the-2.3etv2 promoter was synthesised from zebrafish genomic DNA with a forward primer 5’- TATAGGGCGAATTGggtaccTTCAGTAAGCAGACTCCTTCAATCA -3’ and a reverse primer 5’- AGCTGGAGCTCCAccgcggTTCGGCATACTGCTGTTGGAC -3’ by Phusion High-Fidelity DNA Polymerase (Thermo Scientific) as an insert for In-Fusion Cloning (Takara Bio ...
-
bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTT-3’) by colony PCR (Vaishnava et al., 2011) using DreamTaq Master Mix (ThermoFisher Scientific) and 0.2μM primers ...
-
bioRxiv - Cancer Biology 2020Quote: ... using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies). The pCS2:runx1 probe was a gift from Leonard Zon ...
-
bioRxiv - Genomics 2020Quote: ... using forward primer (5’-TGATTATCGACATCCCGTCA-3’) and reverse primer (5’-GTCTGGAATCTCATAGGTAG-3’) and run on an ABI 7500 thermocycler (Applied Biosystems). Primer specificity and capture temperature were determined by melt curve analysis ...
-
bioRxiv - Neuroscience 2020Quote: Genomic DNA in the vicinity of the rs7143400 was amplified by PCR using the following primers 5’-GGTTGGGTGTGAATAGGAAT-3’ and 5’-TGCATGCCTGATTTATTTGG-3’ before digestion with Tsp45I enzyme (Thermo Scientific). Finally ...
-
bioRxiv - Microbiology 2021Quote: ... Resulting colonies were screened for plasmid presence by PCR of a portion of the pESC-URA-lpt1 plasmid (Forward primer: 5’-TTGGAAACAGCTCCAAATCC-3’, Reverse primer: 5’ CCCAAAACCTTCTCAAGCAA-3’; ordered from ThermoFisher Oligos) and preserved as glycerol stocks.
-
bioRxiv - Developmental Biology 2022Quote: ... were retrotranscribed from either 5’-CATGCTGCTGGTGGGTGTGCT-3’ or 5’-CCATAAAGCACCGGTGAGCAGAA-3’ endoglin specific reverse oligonucleotides (500 nM) using RevertAid H minus reverse Transcriptase (Thermo Scientific) 10 U/μl in 1X RevertAid H minus Buffer supplemented with 2 U/μl RNAse OUT (Thermo Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... using the ZIKV-F2 (5’-CAGCTGGCATCATGAAGAATC-3’) and ZIKV-R1 (5’-CACTTGTCCCATC TTCTTCTCC-3’) primers for African strain detection (ThermoFisher SCIENTIFIC) or the ZIKV-F1 (5’-CAGCTGGCATCATGAAGAACC-3’ ...
-
bioRxiv - Molecular Biology 2022Quote: ... sgRNA sequences cutting near genomic loci of MLL3 Y4792 (5’-ACTATGGTCATCGAGTACAT-3’) and MLL4 Y5477 (5’-ACGATGGTCATCGAGTACAT-3’) were synthesized by Thermo Fisher’s custom in vitro transcription service ...
-
bioRxiv - Cancer Biology 2019Quote: ... 97-mer oligonucleotides were purchased from Invitrogen and PCR amplified using the primers miRE-Xho-fw (5’ TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’) and miRE-EcoOligo-rev (5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’) according to Phusion High-Fidelity kit (Thermo Scientific). The PCR product (139 bp ...
-
bioRxiv - Plant Biology 2021Quote: The IKU2 promoter was amplified using the primers Prom-IKU2-B4 (5’-ggggacaactttgtatagaaaagttgGGTCTCTCTTGATAACGATTTG-3’) and Prom-IKU2-B1R (5’-ggggactgcttttttgtacaaacttgTGTTCTCTACGTCGGAAGG- 3’) and cloned into pDONR-P4-P1R (Life Technologies). A triple LR Gateway reaction (Life Technologies ...
-
bioRxiv - Developmental Biology 2020Quote: ... PCR was done on1 uL of cDNA using 10 µM of forward and reverse primers (Fw 5’-AAGATTCTCCTGAGCTGGGTC −3’ and Rv 5’-AGTCACTTTAGGTGGCCTTGG −3’, Life technologies) and 1 U Taq DNA polymerase (10342 ...
-
bioRxiv - Immunology 2020Quote: ... or equimolar mix of two human APOBEC3A siRNAs (Silencer 45715 and 45810, respectively, with sense sequences 5’-GACCUACCUGUGCUACGAATT-3’ and 5’-GCAGUAUGCUCCCGAUCAATT-3’, Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) ...
-
bioRxiv - Neuroscience 2021Quote: ... or with a pool of two different USP30 siRNAs (D1: 5’-CAAAUUACCTGCCGCACAA-3’; D3, 5’-ACAGGAUGCUCACGAAUUA-3’, Dharmacon; siUSP30) by using Lipofectamine RNAiMAX (Thermo Scientific) following the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2021Quote: ... and human Oct 4 (For: 5’-CTTGCTGCAGAAGTGGGTGGAGGAA-3’/Rev: 5’-CTGCAGTGTGGGTTTCGGGCA-3’) PCRs were conducted using an ABI 7300 Real Time PCR System (Applied Biosystems). PCR cycling conditions were 95° C for 10 min. ...
-
bioRxiv - Cancer Biology 2021Quote: ... The synthesized cDNA was amplified by EBNA 3C (1F and 1R) primers (5’-GAGAAGGGGAGC GTGTGTTGT-3’, 5’-GCTCGTTTTTGACGTCGGC-3’) by using regular PCR and adding Taq DNA polymerase (ThermoFisher, USA). The components of 25 μL reaction mixture contained 10 μL extracted DNA ...
-
bioRxiv - Immunology 2021Quote: ... against the IAV nucleoprotein (forward: 5’-CAGCCTAATCAGACCAAATG-3’; reverse: 5’-TACCTGCTTCTCAGTTCAAG-3’) were assayed using SYBR Green Master Mix (Applied Biosystems) to confirm viral presence in the maternal lung.
-
bioRxiv - Cell Biology 2019Quote: ... and passaged every 4-5 days with 1 mg/ml dispase (Gibco).
-
bioRxiv - Molecular Biology 2022Quote: ... and passaged every 4-5 days with 1 mg/ml dispase (Gibco).
-
bioRxiv - Neuroscience 2024Quote: ... and passaged every 4-5 days with 1 mg/ml Dispase (Gibco). The human FUSWT line ...
-
bioRxiv - Developmental Biology 2020Quote: ... ToPro-3 (1:1000, Invitrogen). Secondary antibodies used in this study were purchased from Invitrogen and include ...
-
bioRxiv - Neuroscience 2023Quote: ... Differentiation medium was replaced daily on days 4 and 5 by 75:25 KSR:N2 medium (486.5 mL DMEM/F-12 (Thermo Fisher Scientific, 11320033), 5 mL 15 % glucose ...
-
bioRxiv - Cell Biology 2023Quote: ... on homemade coverslip-bottomed dishes were washed and loaded with Fura 2 by incubation with Fura 2 AM (4 μM, 45 min, 37°C, ThermoFisher, Cat# F1221), washed and imaged at room temperature in HEPES-buffered saline solution (HBSS) ...
-
bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...
-
bioRxiv - Immunology 2023Quote: ... CE0-3 SOSIPs were expressed in FreestyleTM 293-F cells (ThermoFisher Cat No. R79007). The modified CH505 transmitted founder Env SOSIPs were chimeras containing the CH505 gp120 and BG505 gp41 domains ...
-
bioRxiv - Biochemistry 2020Quote: ... TFA and 1 μL of this dilution and 1μL of the undiluted peptides from bands 3 and 4 were injected onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm × 5 mm), using a partial loop injection ...
-
bioRxiv - Cell Biology 2023Quote: ... ASCEs were maintained on mitomycin C-treated 3T3-J2 feeder cells (a generous gift from the late Dr Howard Green) in F medium (3:1 [v/v] Ham’s F12 nutrient mixture:DMEM, high glucose (both from Life Technologies) supplemented with 5% FBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
-
bioRxiv - Cell Biology 2024Quote: Isolated CDX2mCherry cells (n=5) mCherry-negative cells (n=4) and hES cells (n=2 pooled) were centrifuged and 200uL Tri reagent (Thermo Fisher Scientific, USA) was added and incubated at room temperature for 15 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto NuPAGE 3-8 % Tris-Acetate gradient gels (ThermoFisher) prior to transfer onto PVDF membrane ...
-
bioRxiv - Immunology 2021Quote: ... Eluted proteins were run on a 3-8% Tris Acetate Gel (Invitrogen) or 4-12% Tris Bis gel (Invitrogen) ...
-
bioRxiv - Neuroscience 2021Quote: ... Lysates were separated by 3%–8% Tris-Acetate SDS-Page (Thermo fisher) and probed against rabbit IP3R1 (1:1000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... samples were run on 3-8% Tris-Acetate gels (Thermo Fisher, 12095655). Prestained protein ladder (Thermo Fisher ...
-
bioRxiv - Genomics 2020Quote: ... The sample was run on a 3-8% Tris Acetate Gel (Invitrogen) for 1 hour at 150 V ...
-
bioRxiv - Molecular Biology 2020Quote: ... nuclear extracts were separated using 3–8% Tris-Acetate gradient gels (Invitrogen). Total proteins on the gel were transferred to a PVDF membrane (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2020Quote: ... gels or NuPage Tris-Acetate 3-8% gels (Invitrogen, Carlsbad, CA, USA). Proteins were transferred using iBlot dry transfer system (Invitrogen ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated using 3-8% Tris-Acetate gels (Invitrogen, Carlsbad, CA) and visualized after western blotting ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were resolved on 3%–8% NuPAGE Tris-acetate gels (Invitrogen) and transferred onto PVDF membranes (Invitrogen ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were then loaded on NuPAGE 3-8% Tris-Acetate gels (Invitrogen). Western blots were performed using iBlot 2 Gel Transfer Stacks PVDF system (Invitrogen) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Samples were separated by NuPAGE 3-8% Tris-Acetate SDS-PAGE (Invitrogen) in duplicate sets ...
-
bioRxiv - Biophysics 2022Quote: Samples were run on a 3-8% pre-casted gels (Thermo Scientific), in 1xTA buffer (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... Nuclear extracts were resolved using 3–8% Tris-Acetate gradient gels (Invitrogen).
-
bioRxiv - Cell Biology 2022Quote: ... Worm extracts were loaded on NuPAGE 3-8% Tris Acetate gels (Invitrogen). Proteins were then transferred onto nitro-cellulose membranes which were incubated with 1 µg/µL primary antibodies in 1X TBS-Tween (TBS-T ...
-
bioRxiv - Genetics 2023Quote: ... Protein was separated on 3 to 8% NuPAGE Tris-Acetate gel (Invitrogen) and transferred to PVDF membrane ...
-
bioRxiv - Biophysics 2023Quote: ... Samples were subsequently loaded onto NuPAGE™ 3–8% Tris-Acetate (Invitrogen) and NativePAGE™ 4–16% ...
-
bioRxiv - Genetics 2024Quote: ... and separated by 3–8% NuPAGE™ Tris-Acetate gels (EA0375BOX, Invitrogen). Then the resolved proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore ...
-
bioRxiv - Biochemistry 2021Quote: ... and/or ToPro-3-3 (1 μM; Thermo-Fisher Scientific, Waltham, MA) for 10 minutes at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was purchased from Life Technologies/Thermo Fisher Scientific ...
-
Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... F(ab’)2-goat anti-rabbit IgG Alexa 555 (Thermo Fisher) was used for dual labeling ...
-
bioRxiv - Neuroscience 2021Quote: ... 10% dimethyl sulfoxide and 2% (w/v) Pluronic F-127 (Invitrogen) in Ca2+/Mg2+ free pipette solution (in mM ...