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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 16000g for 20 min at 4°C and Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) was used to determine cleared lysate concentration ...
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bioRxiv - Plant Biology 2022Quote: ... and immunocomplexes were next incubated for 1h at 4°C with 40 μL of Dynabeads Protein A (Thermo Fisher Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... endogenous PABP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-PABP (clone 10E10 ...
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bioRxiv - Developmental Biology 2022Quote: ... 4°C and supernatant was collected and the protein content was quantified with a BCA kit (Thermo Fisher Scientific, 23225). Equal amount of protein was denatured by mixing with 4x Laemmli buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Protein-DNA complexes were immunoprecipitated by incubating the diluted chromatin overnight at 4°C with 15 μl of Dynabeads Protein-G (Invitrogen) equilibrated with 2 μg of anti-GFP antibody (Takara Bio ...
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bioRxiv - Neuroscience 2024Quote: ... denatured (70°C for 10min) protein samples (1 µg/µl) were loaded onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen) along with Chameleon 800 pre-stained protein ladder (LI-COR) ...
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bioRxiv - Cell Biology 2024Quote: ... Sonicated chromatin was incubated overnight with 6 μg of C/EBPβ or IgG antibody (listed in Table 1) followed by 2h incubation with Dynabeads Protein G beads (Invitrogen). After five washes with LiCl buffer (100mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of a mixture of specific siRNA against c-Myc protein (exon 2 and exon 3, IDs s9129 and s9130, respectively; Ambion) and DOT1L protein (IDs s39011 ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged for 25 mins at 35,000 x g and the supernatant was adjusted to approximately 3 mg/ml protein (Nanodrop 2000 C, Thermofisher Scientific). To capture the SVs for content detection ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
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bioRxiv - Neuroscience 2023Quote: ... We centrifuged homogenates at 21,000 x g for 15 minutes at 4°C to remove cellular debris and determined protein concentrations by BCA assay (ThermoFisher).
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bioRxiv - Systems Biology 2022Quote: ... Proteins were diluted to1%SDS and digested with MS grade trypsin protease and Lys-C protease (Pierce, Thermo Fisher Scientific) overnight at an estimated 1:100 enzyme to substrate ratio quenching with 1% trifluoroacetic acid (TFA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein was transferred (400 mAh, 60 min, 4 °C) in Towbin buffer onto PVDF membrane (Cat. No. 88518, Thermo Scientific). Total protein was quantified with Ponceau S stain ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C by adding 0.3 µg of Pierce trypsin protease (MS grade, Thermo Fisher Scientific), followed by a second incubation with 0.3 µg trypsin for three hours.
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bioRxiv - Immunology 2023Quote: ... added with 50 mM DTT for 10 min at 75 C and loaded onto precast NuPAGETM 4-12% bis-tris protein gels (Invitrogen), or NuPAGETM 12% bis-tris protein gels (these ones were used for LC3 immunoblotting ...
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bioRxiv - Cell Biology 2023Quote: ... Then the cell lysate was mixed with antibodies at 4 °C overnight followed by t he addition of protein G beads (10003D, Invitrogen). Otherwise ...
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bioRxiv - Molecular Biology 2023Quote: ... and supernatants were incubated (2 h, 4°C) with 5 µg antibodies conjugated to 25 µL protein G dynabeads (Invitrogen). Samples were immobilised ...
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bioRxiv - Developmental Biology 2022Quote: Protein lysates were denatured by heating at 70 °C for 30 min with NuPAGE LDS sample buffer (Invitrogen, cat. # NP0007) and NuPAGE reducing agent (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... All FCS measurements were carried out at ∼20 nM fluorescently labeled protein at 20°C in Nunc chambered coverslips (ThermoFisher). The chambers are pre-incubated with poly-lysine conjugated polyethylene glycol (PLL-PEG ...
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bioRxiv - Plant Biology 2023Quote: ... the denatured proteins were removed by centrifugation (4,122 g, 20 min, 4°C, Haraeus Multifuge X3R, Thermo Scientific Waltham, USA). To precipitate polysaccharides and AGPs ...
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bioRxiv - Plant Biology 2023Quote: ... the entry vector was subsequently recombined into the Gateway-compatible plant binary vectors pGWB505(48) for C-terminal fusion with EGFP (Enhanced Green Fluorescent Protein) through Gateway LR reaction (Invitrogen). The 1053 bp native promoter ...
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Identification of resistance mechanisms to small-molecule inhibition of TEAD-regulated transcriptionbioRxiv - Cancer Biology 2023Quote: ... at 4⁰C and centrifuged (18,000 x g and 4⁰C) for 10 minutes prior to protein quantification by BCA assay (ThermoFisher Scientific). Proteins in lysates (1µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Plant Biology 2023Quote: ... and RAF39 driven by the cauliflower mosaic virus (CaMV) 35S promoter and C-terminally tagged with green fluorescent protein (GFP) was achieved using Gateway LR Clonase II (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Immunology 2023Quote: ... intracellular staining was performed for 2 h at 4°C after stimulation for 2 h with PMA/Ionomycin Cell Stimulation Cocktail containing protein transport inhibitors (ThermoFisher). Data were acquired on an Aurora (Cytek Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4°C with the corresponding antibody bound to protein A/G-magnetic beads (Dynabeads, Invitrogen). Immuno-complexes were washed 4 times with IP and twice time with kinase buffer (25 mM Hepes pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... The final SEC-buffer was either supplemented with 2 mM CaCl2 or concentrated proteins were dialysed overnight at 4 °C using 3.5K MWCO Slide-A-Lyzer MINI Dialysis Devices (Thermo Scientific) in 100 mL SEC buffer containing 2 mM CaCl2 ...
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bioRxiv - Genetics 2024Quote: ... pH 8.2 by beating with 0.5 mm glass beads at 4°C and protein concentration was determined by bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Protein was reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were centrifuged at 15,000xg for 15 min at 4°C and the supernatant was pre-cleared with protein G-magnetic Dynabeads (#10004D, Invitrogen) at 4°C for 2h ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins eluted by heating at 95°C for 10 min with Tris Lysis Buffer and LDS Sample Buffer 4X (Invitrogen). For western blots ...
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bioRxiv - Immunology 2024Quote: ... The solid protein was denatured at 95°C for 5 min in NuPAGE LDS Sample Buffer (4X) (Thermo Scientific Inc) containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.5% SDS) and incubated for 12-16 h at 4°C with 25 μL protein G Dynabeads (Invitrogen, 10004D) and 5 μg of antibody (Supplementary table 6) ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 16 hours at 4°C with HA Tag-precoated Dynabeads-Protein G (Invitrogen, Cat.#: 10007D). Then the Dynabeads were rinsed with a high salt buffer (50mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1200 µg of protein were incubated overnight rotating at 4°C with 200 µl of streptavidin beads (Thermo Scientific, 88816) which were acetylated with Sulfo-NHS-Acetate beforehand ...
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bioRxiv - Neuroscience 2024Quote: ... The embryos were then washed several times with blocking solution and incubated for 1h in blocking solution containing a secondary antibody Alexa Fluor 647 (Thermo Scientific, USA, #A-21244, 1:250) or Alexa Fluor 488 (Thermo Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... a small fraction of peptides from all samples was pooled and fractionated using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (Pierce, Thermo Fisher Scientific, San Jose). Briefly ...
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bioRxiv - Systems Biology 2024Quote: ... then 80µg total peptide are directly deposed on a Pierce™ High pH Reversed-Phase Peptide Fractionation column (Thermo Fisher Scientific, Waltham, United States) and centrifuged at 3000g for 2 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The desalted peptides were dried and resuspended in HPLC-grade water for quantification using the Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Resulting tryptic peptides were resuspended in MS-grade water and were quantified using a Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher cat #23290). Prior to MS ...
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bioRxiv - Neuroscience 2024Quote: ... An aliquot of the desalted peptides was taken for the proteome analysis and the desalted peptides were dried in a centrifugal Savant SpeedVac (Thermo Fisher Scientific, Waltham, USA).
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bioRxiv - Developmental Biology 2021Quote: ... except that the blocking solution contained 10% normal horse serum (Gibco) and 0.2% Triton in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... and blocked in blocking solution (PBS + 1% bovine serum albumin (Affymetrix) + 5% normal donkey serum (Jackson Immuno Research ...
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bioRxiv - Developmental Biology 2020Quote: ... Tissues were incubated in blocking solution consisting of PBS (Gibco, #14190094) with 4% goat (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... then blocked with 80 µl SuperBlock (PBS) Blocking Buffer (Thermo Scientific) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... After a blocking step with PBS containing 5% chicken serum (Gibco), cells were incubated with anti-YFV-C and anti-YFV (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were blocked with StartingBlock™ (PBS) Blocking Buffer (Invitrogen) for 30 min at room temperature with gentle agitation ...