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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and quantified by fluorometric peptide assay kit (Thermo Fisher Scientific) prior to mass spectrometry analysis.
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bioRxiv - Cell Biology 2024Quote: ... Peptides were reacted with TMTpro 16plex reagents (Thermo Fisher, 44520) in 40% v/v acetonitrile and incubated for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were loaded on a C18 trap column (Thermo Scientific), washed with buffer A (0.1% formic acid ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were analyzed on an OrbiTrap QExactive (Thermo Fisher Scientific) as previously described (Dubois et al ...
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bioRxiv - Plant Biology 2024Quote: ... Peptide samples were analyzed using an EASY-nLC1200 (Thermo Fisher) coupled to an Exploris 480 (E480 ...
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bioRxiv - Biochemistry 2024Quote: ... and desalted using a Pierce Peptide Desalting Spin Column (ThermoFisher). The eluate was again evaporated to dryness in a SpeedVac prior to high pH reversed-phase peptide fractionation.
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bioRxiv - Biochemistry 2024Quote: ... Peptide volume was reduced using a SpeedVac concentrator (ThermoFisher Scientific). Sample aliquots of 200 µg were lyophilized for multiplexing ...
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bioRxiv - Biochemistry 2024Quote: ... high pH reverse peptide isolation kit (Thermo Fisher Scientific, USA), iRT (indexed retention time ...
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bioRxiv - Biochemistry 2024Quote: ... high pH reverse peptide isolation kit (Thermo Fisher Scientific, USA), iRT (indexed retention time ...
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bioRxiv - Neuroscience 2024Quote: 1) We purchased peptide desalting spin columns (Pierce, Thermo Scientific) and used them as per manufacturer protocol.
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bioRxiv - Microbiology 2024Quote: Peptide fractionation kit was purchased from Fisher Scientific (Cat. 84868). A total of 40 µg pooled peptides were processed according manufactures instruction ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and resuspended in PBS containing an amine-reactive viability dye (Live/Dead Fixable Cell Stain, #L34960, Thermo Fisher Scientific, Houston, TX, USA) for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were reconstituted in 50 mM TEAB and their concentrations were determined using the Pierce™ quantitative fluorometric peptide assay (Thermo Fisher Scientific, Waltham, MA). Twelve micrograms of peptides were labelled with TMT labels (6-plex ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten labeled samples in each of TMT 10-plex sets were pooled together and 80 µg of labeled tryptic peptides were then fractionated using a Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo-Fisher Scientific, San Jose, CA) with a 9-step isocratic elution ...
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bioRxiv - Molecular Biology 2020Quote: ... Precleared nuclear protein lysates (500 μg) were incubated with 20 μl of anti-c-MYC tag antibody coupled to magnetic beads (Thermo Scientific). After two hours ...
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bioRxiv - Cell Biology 2019Quote: ... the cell lysates were centrifuged at 12,000 g for 15 min at 4 °C and the supernatant was precleared with Pierce Protein A/G Magnetic Beads (88802, Thermo scientific) for 15 min on ice and then incubated with 2 μg anti-CRIPT antibody at 4 °C for 2 hours and with Pierce Protein A/G Magnetic Beads for another 2 hours ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 isolated T cells were then stimulated at 37°C with eBioscience™ Cell Stimulation Cocktail plus protein transport inhibitors (Invitrogen) for 6 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and bound proteins eluted by heating at 70200°C for 10 minutes in 20 μl 4x Nupage LDS loading buffer (Invitrogen, NP0007) containing 5% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted supernatants were pre-cleared for 1 hr at 4 °C on a rotator with 50 μL of Protein-A Dynabeads (Life Technologies) pre-blocked overnight in ChIP buffer + 0.01% SDS and 100 μg ultrapure BSA (Ambion) ...
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bioRxiv - Biophysics 2022Quote: Samples were heated at 95°C for 5 minutes before loading the onto the PAA-gel (marker: PageRuler Plus Prestained protein ladder (Thermo Scientific)) ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Cancer Biology 2019Quote: ... Total cell extracts were then centrifuged at 10000g at 4°C and supernatants were assessed for protein concentration using the BCA assay kit (Pierce, Thermo Scientific) according to the manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... Gastric homogenates were centrifuged at 13,000 × g at 4°C and the supernatants collected for analysis by the Qubit™ protein assay (ThermoFisher Scientific) and ELISA.
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bioRxiv - Developmental Biology 2019Quote: ... The lysate was centrifuged at 16,000 X g for 15 min at 4°C to remove cell debris and proteins were quantified using a BCA assay kit (Pierce/Thermo Scientific). Thirty or 40 µg of protein was resolved on Mini-PROTEAN TGX precast 12% SDS-PAGE gels (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Synthetic Biology 2021Quote: ... Immunoprecipitation was performed for 16h at 4°C on a rotating platform by incubating with Dynabeads protein-G (Thermo Fisher Scientific) conjugated with antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... These proteins were then reduced at 90°C for 10 min and loaded into a 4-12% bis-tris gel (Life Technologies). This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunoprecipitation was performed for 16h at 4°C on a rotating platform by incubating with Dynabeads protein-G (Thermo Fisher Scientific) conjugated with 5 μg of one of the following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... and 30 μg (iPSCs-MNs) protein of each sample was denatured for 10 min at 70 °C in LDS sample buffer (Invitrogen NP0008) containing 2.5% 2- mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... The ATG8-PE (lipidation) band was confirmed by incubating protein samples at 37°C for 1 hour with Streptomyces chromofuscus phospholipase D (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were then boiled at 100 °C for 10 min and 5 μl of each sample was used for protein quantification using the MicroBCA assay (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Biotinylated proteins were isolated by incubating the samples with equal total protein amounts overnight at 4°C and gentle agitation with 30 μl of Neutravidin-magnetic beads (Fisher Scientific, Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Thawed cell pellets were heated 5 min at 95°C before measuring protein contend by using a micro-BCA assay (Pierce, ThermoFisher Scientific) and a NanoDrop One spectrophometer (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants of lysates were mixed with anti-c-Myc antibody (Nacalai tesque) attached to Protein G DynabeadsTM (Thermo Fisher, 10004D). After incubation for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged (14000 g, 30 min, 4 °C) and protein concentration in the supernatant was determined with the BCA method (ThermoFisher, Germany). After boiling with 4x Laemmli buffer (1 M Tris pH 6.8 ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 37°C to OD600 0.6 and protein expression induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; ThermoFisher Scientific) for 4 hr at 30°C (3CL-pro and RBD) ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... then the supernatant was diluted 10x and pre-cleared for 30 min at 4 °C with rotation using 5 µl protein A- and G-coupled dynabeads (ThermoFisher Scientific). An input sample was taken ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hr at 4°C, then proceeded for immunoprecipitation with specific antibodies (Rab5A, Rab 11 and Rab7) and Protein A/G bead (Thermo Fisher) for overnight at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Microbiology 2019Quote: ... and denatured (10 min, 95 °C) Approximately 7.5 µg of total protein (as determined by A280 on a Nanodrop; Thermo Fisher Scientific) was loaded onto 8-16% TGX gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were centrifuged (14.000x g, 10 min at 4°C) and soluble protein concentrations were determined by a Bradford assay (Thermo Fisher Scientific). For digestion ...
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bioRxiv - Microbiology 2020Quote: ... Two and a half micro liter of pBAD-C+SPA+gst sample as well as 5 μL of Spectra Multicolor Low Range Protein Ladder (Thermo Scientific) and 10 μl of samples or controls were loaded on tricine-SDS-gels (Schägger ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C on rotation and for one additional hour with mixed Dynabead protein G and A (20:10 µl, Life Technologies). Beads were washed with TSE- 150 (0.1% SDS,1% Triton ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin with antibody were rotated overnight at 4 °C before 1:40 protein A/G agarose beads (Thermo Fisher Scientific, #20423) were added and incubated for another 6 hrs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The lysate was clarified via centrifugation at 16,100 × g for 10 min at 4°C then total protein concentration was measured using a detergent-compatible Bradford assay (ThermoFisher 23246). Lysate was transferred to a 384-well plate (30 μL × 3 TR-FRET replicates ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Elution fractions were tested on 12% SDS-PAGE gel and fractions containing protein were pooled and dialyzed overnight at 4 °C using Snakeskin dialysis tubing 10K MWCO (Thermo Scientific) in kinase storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Bacterial lysate was clarified by centrifugation at 40,000 xg for 45 min at 4°C and the clarified protein bound to glutathione agarose beads (Thermo Fisher Scientific) at 4 °C for 4 hours with rotation ...