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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Soluble proteins were retrieved after centrifugation at 15000 g at 4°C for 10 minutes and quantified using the Coomassie protein assay (Thermo Scientific). 25 µg of proteins were separated on a NuPAGE 4% -12% gel (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Biochemistry 2022Quote: MT-4 cells with and without lentiviral plasmids were collected by centrifugation (225× g, 5 min, at 20 °C) and proteins were extracted using lysis buffer (RIPA Thermo Scientific, cOmplete™ ...
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bioRxiv - Cancer Biology 2022Quote: ... sonicated chromatin was incubated overnight at 4°C with antibodies that had first been coupled to Protein G beads (Thermo Fisher). After incubation ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Microbiology 2022Quote: ... Filtered supernatant was then concentrated by spinning at 4°C 3000 rcf for 30 min using Pierce Protein Concentrator (ThermoFisher, 88537). Virus aliquots were stored long-term at −80°C ...
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bioRxiv - Microbiology 2022Quote: ... and heated at 70°C for 10 min before being loaded onto NuPAGE 4 to 12% bis-Tris protein gels (Life Technologies). The proteins were transferred using iBlot™ 2 Transfer Stacks (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants were then immunoprecipitated on a rotator for two hours at 4 °C with antibody pre-bound to 17 μl Protein A Dynabeads (Invitrogen, 10001D). The following antibodies was used ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 21,000 x g for 10 minutes at 4°C and protein concentration was determined by Bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Proteins were reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Neuroscience 2022Quote: ... followed by overnight incubation at 4°C with either 10 μl of pre-equilibrated protein A or G Ultralink beads (Thermo Scientific). The resin was washed several times with immunoprecipitation buffer spinning at 700 g for 30 sec ...
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bioRxiv - Neuroscience 2022Quote: ... RIPA-soluble extracts were clarified by centrifugation at 100,000 g for 30 min at 4°C in a Beckman Ultracentrifuge and protein content was measured using a detergent-compatible Bradford assay (Thermo Fisher).
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... cell extracts and purified protein samples were boiled at 95°C for 5 minutes followed by fractionation on NuPAGE Bis-Tris polyacrylamide gels (Thermo Scientific). Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein lysates were heat-denatured for 10 min at 95°C with the addition of Bolt LDS buffer (Thermo Fisher Scientific) and Bolt Reducing agent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 5-10 ug of protein lysates of each sample was denatured for 10 min at 70 °C in LDS sample buffer (Invitrogen NP0008) containing 2.5% 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... The two portions of s-extract were incubated overnight at 4°C with Protein A agarose (PrA) beads (30μL / ml of sample) (ThermoFisher Scientific, 20334) with either anti-β-amyloid 17-24 (4G8 ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then incubated overnight at 4 °C with 5 μg of prebound anti-SP100 or rabbit IgG isotype control protein A dynabeads (Thermo Fisher). The next morning ...
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bioRxiv - Microbiology 2024Quote: ... The purified proteins and protein standards were subjected to a colorimetric bicinchoninic acid (BCA) assay following the manufacturer’s protocol and then stored at −80°C until use (Pierce protein assay kit; ThermoFisher Scientific, USA). The proteins were visualized by running a 4-20% SDS-PAGE (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... at 4 °C and the soluble protein in the supernatant was separated from the debris by centrifugation (13000 rpm, 20 minutes, 4 °C in Thermo Scientific™ Fiberlite™ F12-6 × 500 Fixed-Angle Rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4-20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of total protein were mixed with 4X NuPAGE LDS sample buffer and 10X NuPAGE reducing agent and subsequently denatured for ten minutes at 95°C with a thermo mixer and proteins were separated by size using 4-12% Bis-Tris Plus Gels (Life Technologies) in a SDS Polyacrylamide Gel Electrophoresis System (Life Technologies) ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were performed overnight at 4°C on 40μg of chromatin with antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific). The antibodies used are listed in Table S10 ...
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bioRxiv - Genetics 2023Quote: ... Extracts were incubated with rotation with rabbit polyclonal anti-Piwi antibodies (4 µg per sample) at 4°C for 4h followed by overnight incubation with DynabeadsTM Protein A (50 µl, Invitrogen, 10002D) at 4°C with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µg of the proteins was incubated with equivalent amount of GST-α synuclein fusion protein at 4°C overnight in the present of Ni-NTA beads (Invitrogen). The pull-downs were washed 4 times with lysis buffer and boiled in 2X Laemmli sample buffer for five minutes.
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... then S-glutathionylated proteins were released and prepared for SDS polyacrylamide gel electrophoresis (SDS-PAGE) with reducing sample buffer at 100°C (ThermoFisher #39000); immunoblotting was performed with anti-PDCE2 antibodies (GeneTex #GTX109766) ...
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bioRxiv - Biophysics 2023Quote: ... heated to 95 °C for 5 min and loaded onto the gel with PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) as a reference marker ...
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bioRxiv - Molecular Biology 2023Quote: ... elution of proteins was carried out by incubating the beads at 80 °C with 2.5X NuPAGE LDS sample buffer (NP0007, Thermo Fisher Scientific). Samples were loaded on 4-12% NuPAGE Bis-Tris polyacrylamide gel (NP0322 ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 1 h at 4°C with Pierce™ Protein G magnetic beads (Invitrogen) previously attached to V5 tag antibodies (R960-25) ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 300g for 5 minutes at 4°C and homogenized in cold N-PER Neuronal Protein Extraction Reagent (Thermo Fisher) or cold RIPA lysis buffer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-NIPBL rabbit antibodies followed by 1 h incubation at 4 °C on a rotating wheel with 40 μl of a 1:1 mix of Dynabead protein A (10001D, Life Technologies) and G (10003D ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4 °C with mouse anti-ee antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific( ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were washed twice with PNK buffer and protein-RNA complexes were eluted for 10 minutes at 70°C in 1x LDS buffer (Invitrogen, NP0007) containing 25mM DTT ...
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bioRxiv - Immunology 2023Quote: ... and urine samples were stored at -80°C and analyzed to determine urinary protein concentration by a Bradford assay using the Pierce detergent compatible kit (#23246, ThermoFisher Scientific). The 24-hr urine protein amount was calculated based on 24-hr urine volume and total urinary protein concentration.
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bioRxiv - Physiology 2024Quote: ... Biotinylated proteins were precipitated from the remaining lysate by overnight incubation at 4°C with streptavidin agarose resin (Thermo Scientific, #20353) and recovered in 4x loading sample buffer (0.2M Tris-HCl pH 6.8 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mg of cell lysate was immunoprecipitated overnight at 4° C under rotation with Dynabeads Protein A (1.5 mg) (Thermo Fisher Scientific) and 10 µg anti-ZZZ3 antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... Lysates from the secretomes and intracellular fractions were centrifuged (10 min, 14,000 g, 4 °C) and soluble protein concentrations were determined (Bradford assay, Thermo Fisher Scientific). For digestion ...
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Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... and reduced with DTT then denatured at 70°C for 10min prior to loading 20μg onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, Cat.# NP0315BOX) and separated appropriately (@ 200 constant V) ...
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bioRxiv - Immunology 2023Quote: ... and then incubated for another 5 hours at 37 °C with additional Protein Transport Inhibitor Cocktail containing Brefeldin A and Monensin (eBioscience 00-4980-03, Thermo Fisher). Cells were then fixed and permeabilized with BD Cytofix/Cytoperm Kit (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... snap-frozen lungs were homogenized at 4°C at 20% w/v in protein extraction reagent (T-PER, Thermo Fisher Scientific) containing Halt™ Protease Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Lysates were centrifuged for at 13,000 RPM 15 minutes at 4°C to recover supernatant and quantified for protein concentration by BCA assay (Thermo Scientific, 23225). 70µg of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... and denatured at 70°C for 10 min prior to loading onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, NP0315BOX) and separated half way (15min at 200 constant voltage) ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody or IgG (as control) was added and incubated for 4 hrs at 4°C with Protein G Dynabeads (Life Technologies) for antigen coupling ...
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bioRxiv - Microbiology 2024Quote: ... 100 ng of protein per well was coated overnight at 4°C onto 96-well NUNC MaxiSorp plates (Thermo Fisher Scientific). Coated plates were washed 3x using PBS and subsequently blocked using PBS supplemented with 2% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was precleared for on a rotating wheel at 16 rpm 30 min at 4°C with 10 µl of Protein G dynabeads (Thermo Fisher) that were previously washed with 100 µl of RIPA buffer (10□mM Tris–HCl pH 8.0 ...
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bioRxiv - Genomics 2024Quote: ... Proteins were then digested by 1h incubation at 55°C with 4 μl of 20 mg/ml Proteinase K (Ambion, AM2546). DNA was then recovered by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Neuroscience 2020Quote: ... 50 μL CSF was mixed with 50 μL of an internal standard mixture containing stable isotope-labelled peptides (JPT Peptide Technologies GmbH, Berlin, Germany; Thermo Fisher Scientific Inc. Waltham, MA, USA), 13C-labelled ubiquitin (Silantes ...
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bioRxiv - Cell Biology 2023Quote: ... in 2% BSA in PBS for 5 min and in Reaction Cocktail solution (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 25 mM Cooper reactive, Alexa FluorTM 488 Azide ThermoFisher A10266, and 62.38 mM L-Ascorbic Acid) for 30 minutes ...