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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Following blocking with 5% normal goat serum (Invitrogen, 16210- 072) for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... in Superblock TM blocking buffer in TBS (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... blocked with SuperBlock Blocking Buffer in PBS (Thermo Scientific, 37515), and immunoblotted following the manufacturer’s protocol using an antibody against Atg5 (Novus ...
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bioRxiv - Microbiology 2024Quote: ... After blocking in Blocker Casein in PBS (Thermo Scientific, #37528) for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then stained with primary antibodies overnight at 4 °C and then Alexa fluorophore-conjugated secondary antibodies (Invitrogen) for 1 hr at room temperature.
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bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
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bioRxiv - Microbiology 2021Quote: ... NPC1 C domain and NPC1 I domain) and the viral protein constructs were co-transfected using Lipofectamine 2000 (Invitrogen) following the manufacture instructions.
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bioRxiv - Cell Biology 2020Quote: ... 4°C) and dialyzed using Slide-A-Lyzer Dialysis Cassettes (3,500-D protein molecular weight cutoff; Fisher Scientific, 68035) against buffer D (20mM Hepes-KOH pH 7.9 ...
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bioRxiv - Cell Biology 2022Quote: ... The endogenous antibodies were added for 4 °C overnight reaction before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Cell Biology 2019Quote: ... boiled for 5 min at 90°C and loaded on NuPAGE Novex 4–12% Bis-Tris Protein Gels (Invitrogen). Gels were then fixed for 30 min at RT in fixing solution (40% [v/v] methanol ...
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bioRxiv - Biochemistry 2019Quote: ... Imidazole was removed by desalting the protein solution at 4°C using Zeba spin 7K desalting column (ThermoFisher Scientific) into 50 mM potassium phosphate buffer pH 7.3 ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM PMSF) and precleared for 1 hour at 4°C with 25 μl of protein G Dynabeads (Invitrogen). The precleared chromatin solution (1.5 × 106 cells ...
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bioRxiv - Neuroscience 2020Quote: ... Capture proteins were eluted by heating (98°C) the beads in 100 μl of SDS-PAGE sample buffer (ThermoFisher) for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Tubes were incubated for 1 hour at 4°C and then protein A/G agarose magnetic beads (Thermo Scientific) were added for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were precleared by incubation for 1 hour at 4°C on a rotator with 10μl Protein G magnetic beads (ThermoFisher). The indicated antibody was incubated in 600μl IP buffer containing 1μl 10mg/ml acetylated BSA and 5μl Protein G magnetic beads for 4 hours with rotation at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspension was clarified by centrifugation at 16,000 g for 10 min at 4 °C The clarified protein lysate was mixed with 1.5 mg magnetic beads (Dynabead M-270 Epoxy, ThermoFisher) conjugated with in-house GFP nanobody (Fridy et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromatin was clarified by centrifugation at 21,000 g at 4°C for 10 min and precleared with 80 µl prewashed Dynabeads protein A (ThermoFisher) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant containing the 4°C oligomers were assayed for protein content using the BCA kit (Thermo Fisher Scientific). For control experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... denaturated 5 min at 95°C and finally loaded on NuPAGE 4-12% Bis-Tris protein gels (Life Technologies) and electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Genomics 2020Quote: ... The chromatin was then cleared by centrifugation (12,000 g for 10 minutes at 4°C) and 80 μL of protein G DynaBeads (ThermoFisher, washed 3 times in cold ChIP buffer right before use ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates were then cleared by centrifugation at 40,000g at 4 °C for 20 min and flowed through columns packed with protein-A Sepharose beads (Invitrogen) to deplete the endogenous antibodies ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentrations of cell lysates were measured after centrifugation at 20,817g for 10 minutes at 4 °C using a Micro BCA Protein Assay Kit (Invitrogen). 50 μg of total proteins were analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin was incubated with antibodies overnight at 4°C and equal amounts of Protein A and G Dynabeads (Invitrogen), pre-blocked with BSA (1 mg/ml) ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were digested overnight (16 hours, 600 RPM) with Trypsin/Lys-C Protease Mix (Thermo Scientific, cat. No. A40009) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Immunology 2024Quote: ... All supernatants were harvested via centrifugation and the proteins purified using CaptureSelect C-tag affinity matrix (Thermo Fisher Scientific) on an ÄKTA Pure FPLC system (Cytiva) ...
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Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: ... Diluted samples were subjected to c-di-GMP ELISA kit and Pierce™ 660nm protein assay reagent (Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Debris was pelleted at 15,000 RCF for 15 min at 4°C and protein concentration determined using a BCA assay (Invitrogen). Protein (50 μg per sample ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with either horseradish peroxidase (HRP)-conjugated anti-c-myc antibody (1:5,000) (Thermo Fisher Scientific) or HRP-conjugated anti-GFP antibody (1:5,000 ...
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bioRxiv - Biophysics 2023Quote: ... The protein was incubated overnight at 4°C with stirring with 4× molar excess AL594 or AL647 maleimide (Invitrogen). The labeled protein was concentrated and buffer exchanged into 20 mM Tris pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Equivalent amounts of protein (∼2 mg) were used for immunoprecipitation with Pierce anti-c-Myc Agarose (Thermo Scientific, 20169), anti-Flag Affinity Gel (Bimake ...
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bioRxiv - Neuroscience 2023Quote: ... or HEK293T cells (0.5 mg) were precleared for 30 minutes at 4°C using Protein A/G Sepharose beads (ThermoFisher). Precleared lysates (equal amounts of protein ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Immunology 2023Quote: ... Protein extracts were stored at -80°C and their concentration was estimated using Bicinchoninic acid (BCA) assay (Thermo Scientific). For sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein extracts were centrifuged at 14,000 x g at 4°C for 20 minutes and quantified with Pierce BCA Protein Assay Kit (ThermoFisher). 10 μg or 20 μg of proteins were separated by electrophoresis in denaturing SDS polyacrylamide gel (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: Eluted protein fraction was incubated at 70°C in NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent (Invitrogen) for 10 min before getting loaded to a 4-12% NuPAGE Bis-Tris protein gel ...
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bioRxiv - Genetics 2023Quote: ... using continuous ramping mode from 25 °C to 95 °C with a scanning rate of 0.03 °C/sec and results analyzed using Protein Thermal Shift software version 1.3 (Applied Biosystems). All assays were performed in quadruplicate.
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4°C with anti-mCherry bound to protein A-magnetic beads (Dynabeads, Invitrogen). Immunocomplexes were washed 4 times with IP and twice time with kinase buffer (25 mM Hepes pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (18,253 xg, 5 min, 4°C) and protein concentrations determined by absorbance on a NanoDrop instrument (ThermoFisher). Equal amounts of lysate were mixed with 4x loading dye (2% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed at 4 °C for Scribble and CHIKV proteins using anti-Scrib (1:500, Invitrogen PA5-54821), anti-E2 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatants were isolated by centrifugation at 12,000 × g at 4°C for 30 min and then mixed with 30 μl of Protein G Dynabeads (Invitrogen) that had been preincubated with antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... 1200 µg of protein was incubated overnight rotating at 4°C with 200 ul of Streptavidin beads (Thermo Scientific) which were acetylated with Sulfo-NHS-Acetate beforehand ...
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bioRxiv - Cancer Biology 2019Quote: ... Three 15 amino acid peptides (P1, P3 and P4) and one 14 amino acid peptide (P2) were synthesized (Thermo Fisher Scientific, Rockford, IL). The purity of the peptides was >95% and each peptide was modified by N-terminal acetylation and C-terminal amidation to enhance their stability.
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bioRxiv - Biochemistry 2021Quote: Fluorescence signal was measured from biotinylated peptides (biotin-NHS or biotin-SS-NHS) using Quantitative Fluorometric Peptide Assay (Thermo Scientific Pierce, cat.no.: 23290) and following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit, Thermo Fisher Scientific, #84868) and subsequently dried using vacuum centrifugation.
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bioRxiv - Biochemistry 2023Quote: ... The pooled TMT-labeled peptide sample was then fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific 84868). We followed the manufacturer’s instructions except that the percentage of acetonitrile used in the elution buffers and the subsequent concatenation strategy was performed as described below ...
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bioRxiv - Neuroscience 2023Quote: ... and the presence of FRα-binding peptide in the nuclear fraction was shown by western blot analysis using the antibody against FRα1-binding peptide (Thermo Fisher, ref: PA5-101588).
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...