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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Real-time PCR reactions were set up with a SYBR green power up kit (Applied Biosystems) on an Applied Biosystems 7500 Real Time Quantitative PCR System workstation ...
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bioRxiv - Synthetic Biology 2023Quote: ... a qPCR approach was set in a QuantStudio™ 5 Real-Time PCR system (ThermoFisher Scientific). Expression levels of the aadA gene ...
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bioRxiv - Physiology 2024Quote: ... and qPCR reactions were set up and performed on the Quant 3 PCR System (Applied Biosystems) using PowerUp Sybr Green Master Mix (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed Real Time quantitative PCR (RT-qPCR) on the Bio-Rad CFX connect Real-Time PCR instrument with SYBR™ Green PCR Master Mix (Thermo Fisher Scientific). We computed the fold changes of the target genes by normalizing to the two reference genes 16S rRNA gene and gyrA with against of their geometric mean81 ...
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bioRxiv - Microbiology 2020Quote: ... These primers were earlier used for conventional PCR and thus optimized prior to the q-PCR SYBR Green assay using RT-PCR system 7500 (Applied Biosystems, CA, USA). The reaction mixture was prepared using the Power SYBR Green PCR Master mix 2X kits (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... Gene expression levels were measured by reverse transcription quantitative PCR (RT-PCR) using PowerUp SYBR Green PCR Master Mix (Thermo Fisher Scientific, A25742). Primers are listed in the Supplementary Table S3 and ΔCt was calculated relative to Actb gene while relative gene expression levels were quantified using ΔΔCt method normalizing to unstimulated groups ...
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bioRxiv - Cancer Biology 2020Quote: ... Each cell line was transfected for 72 hours with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion ...
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bioRxiv - Cancer Biology 2020Quote: Capan-1 and Hs 766T were transfected for 72 hours with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion ...
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bioRxiv - Molecular Biology 2019Quote: Cells at 60% confluence were transfected with 40 nM of mirVana™ miRNA mimic of miR-9-5p (miR-9-5p) (4464066, Ambion, Carlsbad, CA) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... AML cell lines U937 (FLT3-negative, miR-155low) and HL60 (FLT3-WT, miR-155low)(24, 25) were maintained in minimum essential medium-α medium (Gibco, Carlsbad, USA). MOLM-14 (FLT3-ITD+ ...
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bioRxiv - Neuroscience 2023Quote: Primary cortical neurons were transfected in triplicates with 100ng of pmiR-Glo-plasmids containing wildtype or mutant 3’UTRs of Mdga1 or Shank2 together with pre-miR-218-5p duplex (0.3125pmol for Mdga1 and 1.25pmol for Shank2, Ambion Pre-miR-218-5p Precursor) or respective quantities of pre-miR negative control duplex (Ambion ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative RT–PCR was conducted using PowerUp SYBR Green Master Mix (Applied Biosystems) and measured on an QuantStudio 3 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... The TaqPathTM COVID-19 CE-IVD RT-PCR kit (ThermoFisher Scientific, Waltham, US) was used to identify the presence of SARS-CoV-2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time RT-PCR analysis was performed using SYBR Green (Invitrogen, KCQS00). Primer sequences are as follows:
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bioRxiv - Molecular Biology 2021Quote: ... Data were analysed by the StepOne Plus RT PCR software v2.1 (Applied Biosystems) and normalized to TATA Binding Protein (TBP ...
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bioRxiv - Cancer Biology 2019Quote: ... Reaction mixes were assembled with SYBR Green RT-PCR master mix (Thermo Fisher), primers ...
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bioRxiv - Neuroscience 2019Quote: ... using the Invitrogen SuperScript III First-Strand Synthesis System for RT-PCR (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Total RNA for quantitative RT-PCR assays were extracted using Trizol reagent (Invitrogen). Assays were performed with Kapa SYBR Fast qPCR master mix (Roche ...
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bioRxiv - Bioengineering 2021Quote: ... Gene expression was performed by real-time RT-PCR using SYBR green (Invitrogen) and selected primer pairs (Table S1) ...
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bioRxiv - Neuroscience 2020Quote: ... Semi-quantitative RT-PCR was conducted using a QuantStudio system (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed with PowerUP™SYBRGreen Master Mix (Applied Biosystems). The primers used are listed in Table S1 ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-PCR was performed on QuantStudio 12K Flex (Applied Biosystems, MA, USA) using a Platinum SYBR Green qPCR SuperMix-UDG (11733046 ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed on the 7500 Real-Time PCR System (Applied Biosystems) with SYBR Green fluorescent probes and the following conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... The reverse transcription (RT-PCR) was performed with “Superscript II Reverse Transcriptase” (Invitrogen), according to company instructions ...
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bioRxiv - Physiology 2022Quote: ... RT-PCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems) on a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... qPCR was performed in a StepOnePlus RT PCR System (Applied Biosystems, ThermoFisher Scientific) using TaqMan™ Fast Advanced master mix and TaqMan™ FAM probes (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... and reverse transcribed using the ThermoScript RT-PCR system (ThermoFisher Scientific, Waltham, MA). qPCR was performed using the SYBR Premix Ex Taq kit (TaKaRa Bio USA ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was carried out using SYBR green Fast PCR mastermix (Life Technologies) on an ABI 7500 Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA synthetized by SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen) and SensiFAST SYBR No-ROX kit (Meridian Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... Reaction mixes were comprised of SYBR Green RT-PCR master mix (Thermo Fisher), primers ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR experiments were run on a QuantStudio 6 Flex System (Applied Biosystems). mRNA levels were calculated relative to the housekeeping gene ...
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bioRxiv - Microbiology 2021Quote: ... we used SuperScript® III First-Strand Synthesis System for RT-PCR (Invitrogen) to perform reverse transcription on 3 μL volume of RNA according to the standard protocol of the manual ...
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bioRxiv - Microbiology 2021Quote: ... the TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems, Thermo Fisher) was used to amplify total RNAs ...
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bioRxiv - Microbiology 2021Quote: ... the TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems, Thermo Fisher) was used to amplify total RNAs ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative RT-PCR was performed using Taqman probes commercially available from Applied Biosystems targeting GAPDH (Mm99999915_g1 ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR was performed using the Superscript III first-strand synthesis kit (Invitrogen) and cytochrome gene-specific primers (Table S1 ...
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bioRxiv - Biophysics 2021Quote: ... using the SuperScript First-Strand Synthesis System for RT-PCR (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Real time RT-PCR was performed using the qPCR Master Mix kit (Thermofisher) and the WDR81 Forward/Reverse primers ...
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bioRxiv - Biochemistry 2020Quote: ... The melting curves were generated using a StepOne + RT PCR machine (Life Technologies) with a gradient from 25 °C to 95 °C utilizing 1 °C steps ...
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bioRxiv - Biochemistry 2020Quote: ... RT-qPCR was completed using a QS5 384-well PCR system (Applied Biosystems) using Power Sybr Green Master Mix and the manufacturer’s Comparative Ct with melt standard method ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative PCR (RT-qPCR) was performed using SYBR Green (Thermo Fisher Scientific, 4367659) with Applied Biosystems 7900 or StepOnePlusTM following manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... Quantitative RT-PCR was performed using Quantstudio 5 (Thermo Fisher Scientific, Berlin, Germany) and GoTag®qPCR Master Mix with SYBR green fluorescence (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed using PowerSYBR Grenn PCR Master Mix (Fisher scientific, #10658255). The following primer were used:
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bioRxiv - Neuroscience 2022Quote: ... RT-PCR were performed with PowerUp™ SYBR™ Green Master Mix (ThermoFisher) with 45 cycles and PCR products were loaded into 2% agarose gel and run at 75 v for 5.5 h ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-qPCR was performed with Power SYBR Green PCR master mix (Applied Biosystems) using 7900HT Fast Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... Real time RT-PCR was performed using the SYBRTM Green mastermix (Applied Biosystems). Simple relative quantification of target gene expression normalized to actin was performed using the 2 method [129] ...
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bioRxiv - Biochemistry 2020Quote: ... Quantitative RT-PCR was performed using Fast SYBR Green Master Mix (ThermoFisher Scientific). C4H was amplified using the primer set of CC3258 and CC3259 (Supplementary Table S1) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and DBDs amplified (2-step RT-PCR) using Platinum Taq DNA Polymerase (Invitrogen) and the primers listed in Table S-M1a ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed on a StepOnePlus Real Time PCR System (Applied Biosystems) with the standard protocol (2 h ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-PCR was performed using TaqMan Reverse Transcription Reagents (N808-0234, Applied Biosystems) as described by the manufacturer ...