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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Viral N protein was detected using a rabbit anti-SARS-CoV N antibody [32] as a primary antibody with AlexaFluor 568 anti-rabbit IgG or anti-rabbit IgG-HRP (Thermo Fisher) as secondary antibodies together with DAPI to stain the nucleus by indirect immunofluorescence as described previously [33].
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bioRxiv - Microbiology 2020Quote: ... and the RBD-mFc and NTD-mFc were purified using protein A agarose (ThermoFisher Scientific). In order to obtain RBD-SD1 and NTD ...
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bioRxiv - Microbiology 2020Quote: ... the spike-in culture was aliquoted in a 1:2 ratio with RNAlater™ solution (Invitrogen) on ice and stored at -80 °C.
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bioRxiv - Genomics 2020Quote: ... 2 µl External RNA Control Consortium (ERCC) spike-in controls (ThermoFisher Scientific, Waltham, MA, USA, 4456740), 2 µl Library Prep Control (LP ...
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bioRxiv - Cancer Biology 2020Quote: ... each containing an equal amount of ERCC ExFold RNA Spike-In Mix 2 (Ambion, ref. 4456739), was used ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of the purified RNA was added of ERCC RNA Spike-In Mix 2 (ThermoFisher Scientific #4456740 ...
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bioRxiv - Molecular Biology 2023Quote: ... Inputs were spiked with 2 µL of 1:10 diluted ERCC Spike-in mix (ThermoFisher #4456740) and cleaned using a Zymo RNA cleanup kit (Zymo Research #R1016 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% v/v (FCS) and 2 mM L-glutamine (Gibco). Where indicated ...
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bioRxiv - Immunology 2023Quote: ... Samples were stained in FACS buffer (PBS containing 2% FCS (Thermo Fisher) and 2 mM EDTA (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... or gut organoids (n=2) were lysed with Trizol (Ambion) and RNA was extracted using the Direct-zol Miniprep kit (ZymoResearch ...
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bioRxiv - Microbiology 2019Quote: ... N-2 supplement (1:200; catalog number 17502-048; Invitrogen), 20 ng/ml fibroblast growth factor (catalog number 4114-TC-01M ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μL of First Strand buffer (Invitrogen, P/N Y02321,) 0.5 μL of 0.1 M fresh DTT ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 1X N-2 supplement (Thermo Fisher, Cat#17502048), 1X B-27 supplement without vitamin A (Thermo Fisher ...
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bioRxiv - Genomics 2022Quote: ... 3 μM CHIR99021 (Stemgent) and 0.5× N-2 Supplement (Gibco)) which was changed daily for the cells ...
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bioRxiv - Immunology 2020Quote: ... with 2-(N-morpholino) ethanesulfonic acid (MES) buffer (Novex, Invitrogen) at 150V for 60 to 90 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 % N-2 supplement (100x, #17502048, Thermo Fisher Scientific, USA), 0.5 mM GlutaMAX™ supplement (#35050061 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 % N-2 supplement (100x, #17502048, Thermo Fisher Scientific, USA), 20 ng/mL recombinant human EGF (#PHG0313 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 % N-2 supplement (100x, #17502048, Thermo Fisher Scientific, USA), 0.5 mM GlutaMAX™ supplement (#35050061 ...
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bioRxiv - Neuroscience 2023Quote: ... the differentiation media also contained N-2 (17502001, Thermo Fisher) and B-27 (A3582801 ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-sheep (Thermofisher, SA5-10060). Images were obtained by the Odyssey CLx imaging system and analyzed by Image Studio 5.2.5 (LI-COR).
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bioRxiv - Neuroscience 2022Quote: ... donkey anti-sheep (Life Technologies, A11016). For anti-BrdU immunohistochemistry (IHC) ...
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bioRxiv - Microbiology 2021Quote: ... and 5% sheep blood (Life Technologies) and then covered with a cover glass ...
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bioRxiv - Microbiology 2020Quote: ... 5% sheep blood (Thermo Fisher Scientific) and/or 5 μg/ml hemin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... anti-sheep (A11015, Thermo Fisher Scientific), Alexa555-conjugated anti-mouse (A21422 ...
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bioRxiv - Microbiology 2022Quote: ... and donkey anti-sheep (A21448, ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... or sheep anti-rabbit (Invitrogen: 11203D) beads were added and incubated for 2 hours at 4°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... either sheep anti-mouse (Invitrogen: 11201D) or sheep anti-rabbit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... sheep anti-mouse sPLA2-IIA (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... sheep anti-parvalbumin (PA5-47693, Invitrogen), goat anti-CD31 (AF3628 ...
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bioRxiv - Biochemistry 2023Quote: ... sheep blood cells (Thermo Scientific, Oxoid) were first diluted with 1xPBS ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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bioRxiv - Microbiology 2021Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Plant Biology 2021Quote: ... Final constructs were obtained by transferring all candidate genes subsequently into the plant binary destination vector pAlligator2 (N-terminal triple HA fusions=HA3) (Bensmihen et al., 2004) via LR reactions (Thermo Fisher Scientific). Constructs were transformed into Agrobacteria (Rhizobium radiobacter ...
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bioRxiv - Biophysics 2022Quote: The gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al. ...
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bioRxiv - Biophysics 2022Quote: ... the gene encoding N-terminal His-tagged V2HeR3 was cloned into the EcoRI and XbaI site of pPICZB vector (Thermo Fisher Scientific). For determination of λmax of V2HeR3 WT and mutants ...
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bioRxiv - Microbiology 2020Quote: ... we introduced a stop codon in the pLRB302 plasmid at residue 640 of the envelope ORF (C640Stop) located at the N’ terminal of the cytosolic tail using the Phusion Site-Directed Mutagenesis Kit (Thermo Fisher Scientific) and the following primers ...
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bioRxiv - Immunology 2020Quote: ... FcMBL was biotinylated at the N terminus of the Fc protein using an N-terminal amino-oxy reaction and then coupled to streptavidin superparamagnetic beads (1µM MyOne Dynabead (Thermo Fisher Scientific, USA). Samples were screened using 5 µg of the FcMBL beads ...
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bioRxiv - Plant Biology 2021Quote: The pCR8-ISR1 construct was used to subclone the gene into the pMDC32 (35S cauliflower mosaic virus [CaMV] promoter) or pEarleyGate103 (35S CaMV promoter with an N terminal GFP fusion) vectors using Gateway LR Clonase II (Thermo Fisher Scientific) and then stably expressed into isr1-1 and isr1-2 plants following Agrobacterium tumefaciens (strain GV301)-mediated transformation using the floral dip method (Clough and Bent ...
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bioRxiv - Biochemistry 2022Quote: N-terminal amino acid sequencing for the eOD-GT8 60mer was performed using the Procise Protein Sequencing System (Applied Biosystems, CA, USA). The protein band corresponding to the eOD-GT8 60mer (~50 kDa ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Neuroscience 2021Quote: ... ERCC Spike-In Controls (Invitrogen) were added to RNA samples (input RNA was 100ng per sample ...
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bioRxiv - Cancer Biology 2020Quote: ... ERCC Spike-In Mix (ThermoFisher) was used as an internal control ...
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bioRxiv - Plant Biology 2020Quote: ... ArrayControl RNA Spikes (Applied Biosystems; www.thermofisher.com/applied/biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... ERCC spike-in controls (ThermoFisher) were added to 500 ng of total RNA and ribominus selection was performed using the NEBNext® rRNA Depletion Kit ...
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bioRxiv - Neuroscience 2022Quote: ... ERCC Spike-In Controls (Invitrogen) were added to 100ng of RNA per sample prior to processing with the NEBNext Poly(A ...