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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Serum ELISAs were performed using randomly biotinylated SOSIP trimers using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... The level of 8-Hydroxy-2’-deoxyguanosine (8-OhdG) – a proxy for oxidative DNA damage – was analyzed in corals using DNA Damage Competitive ELISA Kit (Invitrogen) and MyCurveFit.com software as described in (20).
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cell culture supernatants were collected and analyzed for IL-8 release using a commercially available ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: TNF-α concentration in cell culture supernatants was determined using Ready-SET-Go! ELISA kits (ThermoFisher, Cat. 88-7324-88) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... The amount of TNF-α and IL-6 in serum was quantified using commercial mouse ELISA kits (Thermo Fisher Scientific). For ex vivo experiments using splenic myeloid cells ...
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bioRxiv - Immunology 2024Quote: ... The CCL2 levels in tumor lysate was measured by MCP-1 Mouse Uncoated ELISA Kit (Thermo Scientific, #88-7391-22) with comparable sized tumors (∼3 mm diameter) ...
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bioRxiv - Microbiology 2023Quote: Interleukin-6 (IL-6)_ and interleukin-12 (IL-12_ expression levels were detected by ELISA Kits (Thermo Fisher Scientific, USA); the samples analysed via ELISA were either plasma drawn from mice or cell culture supernatants ...
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bioRxiv - Bioengineering 2023Quote: ... The insulin concentration in the collected supernatant is measured through mice insulin ELISA kits (Mercodia, Fisher Scientific, Cat. No. 10124710) and the release index is calculated by normalizing the insulin concentration upon stimulation to the basal value.
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bioRxiv - Cancer Biology 2022Quote: ... Upon treatment the cells were fixed in 4% formaldehyde and in-cell ELISA was performed according to the manufacturer’s protocol (kit 62205, ThermoFisher Scientific). pEGFR antibodies provided in the kit were replaced with pEGFR Y1173 antibodies (44-794G ...
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit ...
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bioRxiv - Bioengineering 2024Quote: ... VEGF or PEDF secretion level in culture medium was measured using the VEGF ELISA kit (Invitrogen or R&D systems) and PEDF ELISA kit (R&D systems ...
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bioRxiv - Microbiology 2024Quote: Multiplex immunoassay was performed using a Luminex bead-based multiplex ELISA kit (ProcartaPlex Mouse Cytokine & Chemokine Panel 1 26plex, reference # EPXR260-26088-901, Invitrogen). Each sample was normalized to the total protein concentration determined by Bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2024Quote: ... and Ser396 residues were similarly determined in total protein extract of hippocampi (diluted at 1:50 in sample diluent buffer) using ELISA kits (Invitrogen, Cat #KHB8051 ...
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bioRxiv - Microbiology 2024Quote: ... and IFN-γ (Cat#BM609, ExtraSensitive) were measured in the different sera according to manufacturer’s instructions by ELISA kits (Invitrogen). Briefly ...
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bioRxiv - Immunology 2024Quote: ... The resulting supernatant was quantified using cytokine specific ELISA kits (IL-1β Invitrogen #88-7013-22; IL-18 Thermo Fisher #BMS618-3 ...
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bioRxiv - Developmental Biology 2024Quote: ... We normalized ELISA results to the total protein content assessed using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). For this we measured serial dilutions of the total protein content of respective cultures.
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were collected at the end of culture and analyzed for IL-2 by ELISA using a kit (cat # 88-7025-76) purchased from Thermofisher Scientific.
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bioRxiv - Immunology 2024Quote: ... IL-6 levels in the supernatant and plasma were determined using a high sensitivity IL-6 ELISA Kit (BMS603HS, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: Estimation of serotonin concentration in the supernatant was done using an Invitrogen serotonin competitive ELISA kit (EEL006, Thermo Fisher Scientific). The assay was carried out according to the manufacturer’s protocol and the concentration of the serotonin was estimated against the serotonin standards carried out in parallel ...
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bioRxiv - Bioengineering 2024Quote: ... The release of interleukin-1β (IL-1β) was monitored using an IL-1β ELISA kit according to the manufacturer’s protocol (Invitrogen, USA). The test was repeated for three times.
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bioRxiv - Cancer Biology 2024Quote: ... TNF-α secretion was measured according to the manufacturer’s instructions using uncoated TNF-α ELISA kits (Invitrogen, 88-7346-88) and absorbance values were read at 450/570nm using Multiskan FC microplate reader (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Genetics 2022Quote: ... Promoter fragments were recombined into the pDONRP4P1r vector in a gateway BP reaction (Invitrogen). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... The two amplicons were assembled with a 5’ CMV promoter amplified from pcDNA3.1 (Invitrogen) by Gibson assembly (New England Biosciences (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplicons were assembled with a 5′ cytomegalovirus (CMV) promoter amplified from pcDNA3.1 (Invitrogen) by Gibson assembly (New England Biolabs) ...
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bioRxiv - Plant Biology 2024Quote: ... in the sense orientation behind the CaMV 35S promoter using T4 DNA ligase (Thermofisher), respectively ...
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bioRxiv - Evolutionary Biology 2024Quote: ... renilla luciferase fused to herpes simplex virus thymidine kinase promoter (pTK-Renilla, Thermo Scientific), and the indicated RIPK ...
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bioRxiv - Plant Biology 2024Quote: ... USA).31 The MpBIR promoter was cloned into GatewayTM pDNOR207 vector (Thermo Fisher Scientific, USA) using the NEBuilder® HiFi DNA Assembly Cloning Kit (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: The AP1 promoter was then cloned in the pCR8/GW/TOPO™ TA (Thermofisher) vector ...
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bioRxiv - Physiology 2023Quote: ... From the tubes 300μl of all samples were transferred to ELISA plate in triplicates and the absorbance was read at 520nm using ELISA plate reader (ThermoFisher™). Optical Density (OD ...
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bioRxiv - Microbiology 2020Quote: ... The ratio between live and death tachyzoites was measured by exclusion technique with trypan blue (4% Gibco, Thermo Fisher, NY, USA), 300 parasites were counted under an optical microscope (AxioObserve Microscope ...
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bioRxiv - Microbiology 2020Quote: ... The ratio between live and death tachyzoites was measured by exclusion technique with trypan blue (4% Gibco, Thermo Fisher, NY, USA), 300 parasites were counted under an optical microscope (AxioObserve Microscope ...
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bioRxiv - Cell Biology 2022Quote: ... volume and proportion of cell death were determined in duplicates (CV <10%) and averaged at each passage using the Countess II Automated Cell Counter (Thermofisher #A27977). To determine the number of cells to plate at each passage ...
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bioRxiv - Neuroscience 2022Quote: Cell viability in HeLa cells treated with pro-death stressors was measured by staining cells with annexin V AlexaFluor-488 conjugate (Thermo Fisher) as described previously 39 ...
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bioRxiv - Neuroscience 2021Quote: ... Percent of cell death was determined by staining with 7 μM Hoechst 33342 and 2 μM propidium iodide (PI) (Invitrogen, USA). PI were diluted in warm neuronal culture medium and incubated for 5 min and then images were taken by a Zeiss microscope equipped with automated computer assisted software (Axiovision 4.6 ...
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bioRxiv - Microbiology 2021Quote: ... and the cell-associated organisms were stained with the anti-Candida antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific). The coverslips were mounted inverted on microscope slides and viewed with an epifluorescent microscope ...
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bioRxiv - Cancer Biology 2020Quote: mRNA associated with 1 to 3 ribosomes “Light polysomes” and mRNA associated with more than 3 ribosomes “Heavy polysomes” were extracted using TRIzol LS (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA (300 ng) and chromatin-associated RNAs (200 ng) were reverse transcribed using random primers and Superscript III reverse transcriptase (Invitrogen). Indicated RNAs were quantified by qPCR on Applied Biosystems qPCR instrument in the presence of SYBR green with the primer sequences listed in Supplementary Table 8 using the ΔCT method ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 20 µM Rho-associated coiled-coil forming protein serine/threonine kinase inhibitor (ROCK-I, compound Y-27632) (ThermoFisher) and transferred to a 6-well ultra-low attachment plate (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... and MUG-Chor1 (CRL-3219) cell lines were purchased from ATCC and cultured in MesenPRO basal medium with an associated growth supplement (Invitrogen), GlutaMAX (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... and peroxidase activity associated with proteins of interest was detected with SuperSignal West Femto Maximum Sensitivity Substrate (#34094, Thermo Fisher).
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bioRxiv - Developmental Biology 2024Quote: ... Detection of purified proteins and associated complexes was performed by immunoblot analysis using chemiluminescence (Immobilon Crescendo/Forte; Thermo Fisher Scientific). Western blots were probed with mouse anti-FLAG (M2 ...
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bioRxiv - Biochemistry 2020Quote: ... and negative selection using the Dynabeads Untouched Human CD4 T Cells kit (Invitrogen 11346D) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Human iPSCs were generated using the CytoTune-iPS 2.9 Sendai Reprogramming Kit (Invitrogen; A16157) as previously described47,61 ...
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bioRxiv - Immunology 2021Quote: ... IFN-γ and TNF-α Cytokine Human ProcartaPlex Panel kits (Invitrogen, Thermo Fisher Scientific) were used following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... IFN-γ and TNF-α Cytokine Human ProcartaPlex Panel kits (Invitrogen, Thermo Fisher Scientific) were used following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was then reverse transcribed by the SMARTer PCR cDNA Synthesis Kit (Clontech ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma IL-6 was measured by immunoassay (ProQuantum Human IL-6 Immunoassay Kit, ThermoFisher) and run on the ABI 7300 (Applied Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... HeLa cell lysate from the 1-Step Human IVT Kit (Thermo Fisher Scientific #88882) and yeast tRNA (Thermo Fisher Scientific #AM7119 ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...