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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... V5-tagged full-length mouse Creb3l3 cDNA was inserted into pcDNA3.1 (Invitrogen); GFP-tagged full-length mouse Creb3l3 cDNA was inserted into pEGFP (GFP-CREB3L3 ...
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bioRxiv - Microbiology 2019Quote: ... V5 tagged constructs were probed with anti-mouse V5 (Invitrogen #R960-25). Blots were developed using the ECL Plus detection reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... These were then tagged using 4 different multiplex TMT isobaric tags (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... corresponding species-specific Alexa-Fluor (488 or 555)-tagged antibodies (Molecular Probes) were incubated for 1h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Specific antibody labeling was detected using fluorescence tagged secondary antibodies from Invitrogen. Details of primary antibodies used are as follows ...
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bioRxiv - Immunology 2021Quote: ... was incubated with purified GST-tagged IKKi (catalog # PV4875; Thermo Fisher Scientific) in buffer A containing 20 mM Tris [pH 7.5] ...
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bioRxiv - Bioengineering 2021Quote: ... tagged with Alexa Fluor™ 647 (Microscale Protein Labelling Kit, Thermo Fisher) (HEL-647) ...
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bioRxiv - Molecular Biology 2021Quote: ... Myc-tagged mouse RHBDL4 and GFP were subcloned into pENTR4 vectors (Invitrogen). Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... V5-tagged proteins were detected with mouse monoclonal anti-V5 antibody (ThermoFisher) at 0.3 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), RFP-tagged strains were taken from the localization database collection (Huh et al ...
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bioRxiv - Immunology 2023Quote: ... tagged ribosomes were eluted with 1mg/ml HA peptide (Thermo Scientific 26184) for 15 minutes at 30°C ...
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bioRxiv - Pathology 2024Quote: ... Wash with PBS and stain fluorescence-tagged rabbit secondary antibodies (Invitrogen, USA) for visualization ...
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bioRxiv - Cell Biology 2020Quote: ... was used at a 1:1000 dilution overnight at 4°C followed by incubation with fluorescently tagged goat anti mouse-A647 and donkey anti-rabbit-A555 secondary antibodies at 1:100 dilution (Invitrogen, Carlsbad, CA). TUNEL staining to detect apoptotic cells was completed prior to antibody stainig per manufactuors instructions (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... The next day the antibody was washed five times in PBST 0.1% with gentle agitation at room temperature (30 minutes each) and fluorescently-tagged by horseradish peroxidase-catalysed signal amplification (Thermo Scientific, T20913)
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... This was followed by staining with the corresponding fluorescent tagged secondary antibodies for 1 hour at room temperature: goat anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 488 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed in PBS and incubated in AlexaFluor-647-tagged goat anti-mouse IgG (1:100; Thermo Fisher Scientific, catalog #A21039), AlexaFluor-647-tagged donkey anti-mouse IgG ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were washed three times with washing buffer (PBS containing 0.7% Triton X-100) before incubation with secondary antibodies (tagged with Cy2, Cy3, or Cy5; dilution 1:500; Life Technologies Inc., USA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... E14.5 Apcdd1 mutant and wildtype control brains were cryosectioned at 16 microns and immunolabeled with Alexa488-tagged Isolectin (1:100; Thermo Fisher Scientific); Calbindin (1:1,1000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, ThermoFisher, cat# A-31573) for 2 hr at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... or Alexa Fluor 594-tagged goat anti-guinea pig IgG (1:500, Thermo Fisher, cat# A-21202, A-21206 and A-11076) for 1.0 – 1.5 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with 1:500 goat anti-rabbit IgG (H+L) secondary antibody tagged with Alexa Fluor 647 (Invitrogen, A-21244) + 1:50 SiglecF-Alexa Fluor 488 (BD ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were transfected with 1μg/well of the HA-tagged Fpn plasmid (Vector Builder, VB220407-1185gaa, Supplemental Figure 1) using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000001).
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bioRxiv - Biochemistry 2023Quote: ... Cells were then again incubated with p65 primary antibody (1:1000) overnight and successively incubated with secondary antibody tagged with Alexa Fluor IgG 555 (#A32732, Thermo Fisher Scientific) as per abovementioned protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the brain sections were washed for 5 min x3 in 0.1% Tween 20/PBS and incubated in fluorescence tagged secondary antibodies for 2 hours at RT: Alexa Fluor 594 anti-rabbit (1:500, ThermoFisher Scientific, A11037), Alexa Fluor 488 anti-mouse IgG1 (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... brain slices were washed 3X in 1X TBS and then incubated for 2 hours at RT with a fluorescent-tagged Alexa Fluor 568 anti-mouse secondary antibody (1:500; Life Technologies A11004). Brain sections were then washed 3X in 1X TBS ...
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bioRxiv - Genetics 2021Quote: ... H3K4me1 Recombinant Polyclonal Antibody (Cat No. 710795, Invitrogen), normal rabbit and mouse IgG (Santa Cruz Biotechnology)
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bioRxiv - Immunology 2022Quote: ... and recombinant murine IL-18 (Thermo Fisher Scientific) at 1 ng/ml unless otherwise specified ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with recombinant murine IL-3 (Gibco, PMC0035) at 1 μg/mL (BaF3) ...
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bioRxiv - Microbiology 2021Quote: ... 50 ng/mL recombinant murine EGF (Fisher Scientific), 1X P/S ...
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bioRxiv - Microbiology 2021Quote: ... 50 ng/mL recombinant murine EGF (Fisher Scientific), 1X P/S ...
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bioRxiv - Microbiology 2020Quote: ... 20 units of RNaseOUT recombinant ribonuclease inhibitor (Invitrogen), and 200 units of M-MLV reverse transcriptase in a final reaction volume of 20 μL ...
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bioRxiv - Neuroscience 2020Quote: ... GS or DA recombinant proteins (970-2527aa) (Thermofisher), GST-AAK1 (1-501aa ...
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bioRxiv - Cell Biology 2022Quote: ... and 20 ng/mL recombinant M-CSF (Gibco). BMDM were lifted and seeded into experimental plates for treatment at passage 1.
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... Recombinant adenoviruses were produced in 293A cells (Invitrogen) and purified via CsCl gradient centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant active GST-TBK1 was from Thermo Fisher/ Life Technologies (#PV3504) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) and SuperScript® III (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 U RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019), 10 U SuperscriptII (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen™) as per manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... 2 U RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019), 10 U SuperscriptII (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins were coated in PBS buffer (Gibco) at the concentration of 1.2 mg/ml of protein per well ...
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bioRxiv - Developmental Biology 2020Quote: ... and RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher Scientific 10777019) according to the protocol provided by the manufactures ...
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Evolutionary conservation of maternal RNA localization in fishes and amphibians revealed by TOMO-SeqbioRxiv - Developmental Biology 2021Quote: ... 0.5 μl of recombinant ribonuclease inhibitor (RNaseOUT, Invitrogen), 0.5 μl of 0.1 M DTT (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... and 0.2 ng/mL recombinant EGF (GIBCO, UK). All cells were cultured at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenoviruses were produced in HEK293A cells (Invitrogen) and purified by CsCl gradient centrifugation ...
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bioRxiv - Immunology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... and BMP4 recombinant protein (Gibco; 5 ng/mL). The media was half-changed every day during the differentiation process ...