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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS; Gibco™), 1% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... GBA was cloned into pcDNA3.1/myc-His A vector (Thermo Fisher Scientific) after digestion with EcoRI and XhoI ...
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bioRxiv - Immunology 2019Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT2 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% heat inactivated Foetal bovine serum (HI-FBS, Life Technologies), 2 mM GlutaMax (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... and 10 % V/V heat-inactivated foetal calf serum (Hi FCS, Gibco). On day 1 ...
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bioRxiv - Microbiology 2021Quote: ... Dynabeads™ His-Tag isolation and pulldown were purchased from Thermo Fisher Scientific.
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT4 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... with 10% v/v heat inactivated foetal calf serum (HI-FCS, Gibco), 1 mM sodium pyruvate and 1X non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% Heat-Inactivated Fetal Bovine Serum (HI FBS; Life Technologies), 10 mL per L of Antibiotic Antimycotic Solution (Penicillin/Streptomycin/Amphoterichin B ...
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bioRxiv - Biophysics 2022Quote: ... blots were incubated with 6x-His Tag Monoclonal Antibody (Thermo Fisher Scientific). As a control ...
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: mouse anti-His (MA1-21315, Thermo Scientific), goat anti-mouse HRP (405306 ...
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bioRxiv - Neuroscience 2022Quote: ... and desmin were inserted into expression vectors pcDNA6 V5-His A (Invitrogen) using BamHI/XhoI ...
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bioRxiv - Microbiology 2022Quote: ... which is RPMI-1640 media containing 10% heat-inactivated (HI)-FBS (Gibco) and 1x penicillin-streptomycin-glutamine (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... were run with 1X Hi-Density TBE Sample Buffer (Thermo Fisher # LC6678) and 1X TBE Running Buffer ...
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bioRxiv - Biochemistry 2023Quote: TOPOVIBL452-579 was cloned as a His-tag fusion into pProEXHTb (Invitrogen). REC114-MEI4 complex and TOPOVIBL452-579 were individually expressed in E ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher), and rabbit polyclonal anti-mCherry (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... His-VASP was bound to HisPur™ Ni-NTA Resin (ThermoFisher #88221), washed twice with 5 CV of wash buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 6x His-tag monoclonal primary antibody (MA1-135; ThermoFisher) overnight at a dilution of 1:750 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher) and mouse monoclonal anti-FLAG (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10-15 mL of His-Pur Ni-NTA resin (Thermo Fisher Scientific) was used per L of cell culture ...
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bioRxiv - Biophysics 2024Quote: ... with 2.5 μl Novex™ Hi-Density TBE Sample Buffer (5X) (Invitrogen). The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, USA) and 100 U mL-1 penicillin–streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10 % heat inactivated Fetal Bovine Serum (HI FBS) (10500064; Gibco) and 1x antibiotic-antimycotic drug (100 units/mL of penicillin ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with 2 µg/ml anti-His (Invitrogen #MA1-21315) over night and blocked for 1hour as described above ...
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bioRxiv - Biochemistry 2024Quote: ... His-tag purification using Ni-NTA resin (Thermo Fisher, Massachusetts, United States) was performed and rubisco was eluted by SUMO tag cleavage with bdSUMO protease (as produced in Davidi et al ...
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bioRxiv - Neuroscience 2022Quote: ... After centrifugation, nuclei pellets were resuspended in 1 ml PBS-RI (PBS, 50 U/mL Rnase-OUT Recombinant Ribonuclease Inhibitor (Invitrogen), 1 mM DTT ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... bacteria resuspended to OD660 0.3 were incubated with 1 µg of recombinant protein in the presence of 1x protease inhibitors (Pierce, Thermo Scientific) in sterile 1.5 ml microcentrifuge tubes with gentle end-over-end mixing for 30 min at 25°C.
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...
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bioRxiv - Zoology 2022Quote: ... Cardiac muscle was detected with a rabbit recombinant polyclonal primary antibody to cardiac troponin I (cTnI, Invitrogen, 1HCLC, 1:600) and a donkey anti-rabbit antibody conjugated to the fluorophore Alexa 647 nm (Invitrogen A31573 ...
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bioRxiv - Microbiology 2023Quote: ... and intracellular staining was performed using recombinant hu-mouse anti SeV-NP antibody conjugated with AlexaFluor-647 (1:1000) (Invitrogen) in combination with either anti-Krt5 (1:500 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR8-KLK4-KLKP1 (DDK tagged) was recombined with pLenti6/V5-Dest™ (Life Technologies) or pAD/CMV/V5-Dest™ (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L) antibody (Invitrogen), before being acquired with BD FACSCanto II (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... sections were incubated with the appropriate Alexa 546-tagged secondary antibody (Invitrogen, Carlsbad, CA) for 2 hrs at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged RNF219 and CNOT1 cDNAs were cloned into pCDNA5/FRT-TO vector (Invitrogen). The expression vectors were then transfected into HEK-293 Flp-In-TRex cells and followed by selection with hygromycin to generate stable cell lines ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-tagged GSK3β constructs were cloned into the pDEST™27 vector (ThermoFisher Scientific) as described previously66 ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged RASSF5 (GST-RASSF5) sequence was transferred into the pDEST15 expression vector (Thermofisher), and then expressed and purified from BL21-A1cells ...
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bioRxiv - Immunology 2022Quote: ... and RAW 264.7 EROS FLAG-tagged cells were maintained in DMEM medium (21969035, ThermoFisher) containing 10 % FBS (F7524 ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... was used to generate Flag-tagged Prom1 clones and deletion mutants in pcDNA3.1+ (Invitrogen). The primers used for cloning are listed in Supplementary Table 1.
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bioRxiv - Biochemistry 2023Quote: ... 6xHis-tagged CapZ was purified as previously described30,49 using HisPur Cobalt resin (Thermo Scientific), and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... N2A cells were transfected with different HA tagged WDR47 variants using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... A fluorescently tagged secondary antibody (Goat anti-rabbit, Alexa Fluor 647, Thermo Fisher Scientific) diluted in the same antibody solution was applied for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
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bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...