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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Nitrocellulose membrane was incubated with His6-tagged Monoclonal Antibody - HRP (Invitrogen) with 1:3000 v/v dilution in PBS-T ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria are tagged with FM-4-64 dye (Thermo Scientific). In the confocal microscope study ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then stained with fluorescently tagged secondary antibodies (Thermo Fisher Scientific Cat# A10037 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The tagged cDNA fragments were subcloned into expression vector pcDNA3.1(+) (Invitrogen). Transient transfection of HEK293 cells by plasmid DNA was carried out by Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... The RFP-tagged organelle markers were purchased from Invitrogen (Carlsbad, CA): CellLight Golgi-RFP BacMam 2.0 (Cat# C10593) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3mM CaCl2 with 250 units of 6xHIS-tagged SUMO protease (ThermoFisher) for scar-free SUMO tag cleavage ...
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bioRxiv - Microbiology 2020Quote: ... hACE2 receptor (hACE2 Recombinant Rabbit Monoclonal Antibody, 1:50, Thermo-Fisher, Cat#SN0754) and DAPI nuclear stain (1:20,000, Invitrogen, Carlsbad, CA). The primary antibodies were detected with goat anti-rabbit-AP developed with Permanent Red warp (mach3 Biocare Medical).
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bioRxiv - Microbiology 2020Quote: ... An aliquot of 50 ng of purified HIV-1 env DNA was used to clone into the pcDNA 3.1D/V5-His-TOPO vector (Invitrogen) and MAX Efficiency Stlb2 competent cells (Life Technology ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 3 hours with 1 μg/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... FACS-sorted cells were suspended at 0.5×106 cells/mL in RPMI 1640 medium supplemented with 10% HI-FCS and 1% PenStrep (10378016; Gibco). CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well ...
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bioRxiv - Neuroscience 2023Quote: The plasmid for heterologous expression of fshr-1 in HEK cells was obtained by directionally cloning the fshr-1 cDNA into the pcDNA3.1/V5-His-TOPO vector (Invitrogen). The cDNA sequence of the fshr-1a gene isoform was amplified by PCR using cDNA from mix-staged wild-type C ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant AtGNL-6XHIS was detected using an anti-HIS (C-term)/AP antibody at a 1:2,000 v/v dilution (Invitrogen) and CDP-start ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then incubated for 1 h at room temperature with the following primary antibodies: mouse anti-His-Tag (dilution 1:1,000, clone HIS.H8, Invitrogen MA121315), mouse anti-Flag (dilution 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with V5-tagged WNT11 and 24 h post transfection proteins were crosslinked for 30 min with 1 mM DSS (Thermo Fisher) in 1 ml PBS + 1mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... For staining FLAG tagged receptors we used anti-FLAG M1 antibody (1:100) labelled with DyLight594 (Thermo Scientific, Cat. no. 46412) dye in presence of 2 mM CaCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated at RT for 1 hr with an anti-mouse secondary antibody tagged with AlexaFluor488 (Thermo Fisher Scientific, A11001) used at 2 μg/mL in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice with filtered PBS and then incubated with AlexaFluor 555-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) and AlexaFluor 488-tagged donkey-anti-mouse (Molecular Probes ...
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bioRxiv - Biochemistry 2023Quote: ... 24 hours later cells were transfected with 2 ug DNA (mCherry tagged PPP1R15A DNA and empty carrier DNA at a ratio of 1:19) using the lipofectamine LTX system (Life Technologies) at a ratio of 3 μl lipofectamine LTX to 1 μl Plus reagent for every nanogram of DNA in 200 μl of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: Fifty wildtype or myc-tagged Sox3 expressing animal pole cell explants were crosslinked for 15 minutes with 1% methanol-free formaldehyde (Life Technologies) and the reaction was quenched with 125 mM glycine in 0.5% PBST (Triton X-100) ...
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bioRxiv - Molecular Biology 2021Quote: ... Then recombinant dsDNA fragment (add to > 400 ng/μl final concentration) or recombinant single-strand DNA (ordered from Invitrogen or IDT ...
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bioRxiv - Developmental Biology 2021Quote: ... fibroblast outgrowths were dissociated and split 1:4 using a recombinant trypsin-like enzyme (TrypLE Select, Invitrogen). Additional fibroblast preparations were commercially available ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/mL of recombinant protein was mixed with SYPRO Orange at a 5X final concentration (Invitrogen), and reactions were supplemented with 50 mM HEPES ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were subsequently incubated by primary antibody 1:500 (GFP recombinant rabbit monoclonal antibody; G10362, Life Technologies) for 48 hours at 4°C followed by three PBS washes ...
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bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into pcDNA3.1D/V5-His-TOPO (Thermo Fisher Scientific). MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS) (Gibco) at 37°C in the presence of 5% carbon dioxide (CO2) ...
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bioRxiv - Bioengineering 2021Quote: ... The anti-His(C-term)-HRP antibody (R931-25, Invitrogen) was used ...
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bioRxiv - Neuroscience 2020Quote: ... cells resuspended in 500µl 2% HI-FBS + PBS (ThermoFisher 12484028), and passed through a 40µm nylon mesh cell strainer (FisherScientific 352235) ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His tag Antibody (MA1-125 21315) was from ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... for over-expression or pcDNA4/TO/myc-his-B (Thermofisher #V103020 ...
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bioRxiv - Neuroscience 2020Quote: The backbone plasmid pcDNA6/V5-His was purchased from Invitrogen Inc ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were added to His beads (Thermo Scientific HisPur Ni-NTA Magnetic Beads ...
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bioRxiv - Immunology 2022Quote: ... we first placed ITPRIPL1 protein with 6x-His antibody (Invitrogen) first and slowly rotated at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... An anti-His-tag antibody coupled to HRP (Thermo Scientific) was used for enzymatic detection ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in 12μl of Hi-Di formamide (Thermo fisher, 4311320), denatured at 95°C for 5 mins followed by snapchill ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% heat-inactivated (HI)-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) or Herculase II Fusion (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) with appropriate primers.
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bioRxiv - Microbiology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS; Life Technologies) and 1X penicillin/streptomycin (pen/strep ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... The Ion PI Hi-Q Sequencing 200 kit (Life Technologies) was used for all sequencing reactions and Torrent Suite™ Software 5.0 (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... secondary anti-6x-His tag monoclonal antibody (Invitrogen, # MA1-21315) at 1:175 dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Cancer Biology 2022Quote: ... Blue Mix Hi-ROX (PCR Biosystems) and StepOnePlus (Applied Biosystems). The data expressed as the ratio between the expression level of IL-6 mRNA and that of Actin ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-HIS antibody (MA1 21315) was purchased from Invitrogen.
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bioRxiv - Bioengineering 2024Quote: Nitrocellulose transfers were stained with anti-His (Invitrogen PA1-983B), anti-SARS-CoV-S (Absolute Antibodies CR3022) ...