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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Recombinant Human Neuropilin 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: 6x-His epitope tag antibody (His.H8) was purchased from Invitrogen. Monoclonal Flag M2 antibody (F1804-50UG) ...
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bioRxiv - Biophysics 2024Quote: ... 7 mL of His-Pur Ni-NTA resin (Thermo Scientific) was equilibrated in Buffer A containing 40 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... and cloned it in the pcDNA3.1/myc-His(-) plasmid (Invitrogen) in frame with a Myc-His tag to obtain the wild-type (WT ...
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bioRxiv - Neuroscience 2023Quote: ... 6x-His Tag Monoclonal Antibody (HIS.H8) (MA1-21315, Thermo Scientific) and HRP-conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG ...
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bioRxiv - Molecular Biology 2023Quote: ... with 5% heat inactivated Fetal bovine serum (HI-FBS) (Gibco). 1 million cells per 100 μl per sample were stained with anti-Flag antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... protein expression from plasmid (derivates of pBAD-His/B (Invitrogen) in all cases except expression of VirF from pCL5 [71] ...
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bioRxiv - Biophysics 2023Quote: ... The medium was supplemented with 10% HI-FBS (Gibco, USA), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... bio-His-PADI4 was incubated with magnetic streptavidin beads (Invitrogen) (4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) in a CO2 incubation at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-human CD14 (11-0149-42, Thermo Fisher Scientific, 1:100), anti-human CD117 known also as cKIT (12-1178-42 ...
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bioRxiv - Bioengineering 2021Quote: ... including Human PD-L1 and PD-1 were purchased from Invitrogen. Human LAG-3 and Na+K+ATPase antibodies were purchased from Cell Signaling Technology and Santa Cruz Biotechnology Inc ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Human/Mouse Myod1 (5.8A) (1:100, Thermo Fisher Scientific, MA512902), Anti-Mouse Myosin Heavy Chain (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... 5 primary and anti-human Alexa 633 (Thermo Fisher; 1:200) secondary antibodies.
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody (100µL 1:500 anti-human IgG HRP, Invitrogen 31410) was added and plates were incubated for 10 minutes at room temperature shaking at 60rpm ...
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bioRxiv - Microbiology 2021Quote: ... The 1/1000 diluted human anti-RBD monoclonal antibody (Thermo Fisher) was added to cells and incubated in a humidified chamber for 1 hr at RT or overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa-Fluor-488-labelled anti-human (Molecular Probes; 1:200 dilution), and 5μg/ml Hoechst 33342 (Molecular Probes) ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal rabbit anti-mouse/human claudin-5 (1:300; Life Technologies), polyclonal rabbit anti-mouse/human ZO-1 (1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal mouse anti-human Rpt6 (PSMC5) (Invitrogen, 2SU-1B8, 1:10000), monoclonal mouse anti-human Rpt4 (Enzo ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 1 % human melanocyte growth supplement-2 (0165, Gibco™). All cell lines were cultured in a humidified 37 °C hypoxic cell culture incubator (Xvivo System Model X3 ...
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bioRxiv - Immunology 2020Quote: ... with 1% (v/v) Human AB serum (Invitrogen, Carlsbad, CA, USA) and 2 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... or monoclonal anti-human NPM1 (IgG1, mouse, 1:500) (325200, Invitrogen) in blocking/permeabilization buffer for 120 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... FITC anti-human/mouse CD45R (1:500, #11-0452-82, Invitrogen), PE anti-mouse CD3ε (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% Human Keratinocyte Growth Supplement (HKGS, #10761364 Fisher Scientific) and 1% Pen/Strep.
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bioRxiv - Cancer Biology 2023Quote: ... rabbit monoclonal anti-human p53 protein SP5 (1:200) (Invitrogen, USA) and mouse monoclonal anti-p63 antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... anti-Human/Mouse MyoD (5.8A) (1:200, Thermo Fisher Scientific, MA512902) and anti-myogenin (F5D ...
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bioRxiv - Microbiology 2023Quote: ... containing 1:400 of Human BD Fc Block (ThermoFisher Scientific, BDB564219) per condition ...
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bioRxiv - Microbiology 2024Quote: ... followed by AlexaFluor 647-conjugated anti-human antibodies (Invitrogen, 1:1,000). Slides were then stained with 1 μg/mL DAPI and mounted in Citifluor (Citifluor Ltd. ...
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bioRxiv - Systems Biology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Immunology 2022Quote: ... stimulated with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and analyzed for cell count ...
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bioRxiv - Immunology 2022Quote: Recombinant soluble ICAM-1 protein was expressed in a Drosophila Melanogaster cells using pMT/V5-His vector with inducible promotor (Invitrogen), purified by sequential two-step affinity chromatography on anti-ICAM-1 antibody (clone YN1.1 ...
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Physiology 2021Quote: ... The resulting cell pellet was resuspended in hypotonic buffer and used for Western blotting using HRP-conjugated anti-His antibody (1: 10,000; Novex, Life technologies).
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Immunology 2021Quote: ... were coated at room temperature for 8 hours with 1 μg/mL 6x-His tag polyclonal antibody (PA1-983B, ThermoFisher), followed by overnight blocking with blocking buffer containing 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... and were maintained in DMEM high glucose (4500 mg/L) supplemented with 10% fetal bovine serum (FBS) (AlphaFBS-HI, Alphabioregen) and 1% Penicillin/Streptomycin antibiotics (Gibco), incubated at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1% FBS (CPS Serum, Cat. No. FBS-500-HI) and 1X penicillin/streptomycin (Gibco, Cat. No. 15140-122), followed by centrifugation in an Eppendorf® centrifuge (model 5810 R ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in a 1:1:1:1 ratio of human receptor:Gαq-Rluc8:β3:GFP2-γ9 DNA constructs prepared in Opti-MEM (Invitrogen) using a 3:1 ratio of TransIT-2020 (Mirus Bio ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Flag-tagged BNIP3 was cloned into a pcDNA3.1 vector (Thermo Fisher Scientific) or a pCDH vector (System Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Tagged constructs were transfected using Lipofectamine 2000 reagent (Life Technologies, CA, USA) following the manufacturer’s instructions using a 1:3 ratio of DNA:Lipofectamine ...
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bioRxiv - Neuroscience 2020Quote: ... and biotin-tagged DNA was pulled down with streptavidin beads (Invitrogen, 65001) and ligated with Illumina paired-end adapters ...
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bioRxiv - Cell Biology 2022Quote: ... dextran polymers tagged with Alexa Fluor™ 680 (3,000, 10,000 MW, Thermofisher) and dextran tagged with fluorescein (40,000 MW ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), while knockout strains were taken from the Yeast Knockout Collection (Invitrogen).