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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfections were performed using Lipofectamine 2000 (ThermoFisher, 11668019) or Calcium Phosphate (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected into AGS via Lipofectamine 2000 (ThermoFisher). siRNA (siNFE2L3#1 ...
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bioRxiv - Cancer Biology 2023Quote: ... or PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen; # K210007), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... extracted using NucleoSpin Plasmid QuickPure Kit (Thermo Fisher Scientific) and confirmed by sequencing at Plasmidsaurus (USA) ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid-based transfection was performed with Lipofectamine 3000 (Invitrogen) for U251 cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids were transformed into ElectroMAX DH10B Cells (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and extracted by GeneJET plasmid miniprep kit (Thermo Scientific).
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bioRxiv - Bioengineering 2024Quote: ... Plasmids were transiently transfected into Expi293 cells (ThermoFisher Scientific). After 5 days ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids were transfected into cells using Lipofectamine 3000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The linearized plasmids were also dephosphorylated with FastAP (ThermoFisher). The products of the HiFi reaction were transformed into NEB10β competent cells (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Ligated plasmids were transformed into DH5α bacteria (Thermo Fisher) and grown overnight on LB plates containing carbenicillin to select for colonies that successfully received the plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNAs were forward-transfected Lipofectamine 3000 (Invitrogen, #L3000001) and following the manufacturers’ instructions.
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bioRxiv - Biochemistry 2024Quote: ... Plasmids are maintained in the bacterial strains: DH5α (ThermoFisher) for standard plasmids ...
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bioRxiv - Synthetic Biology 2024Quote: ... with vacuum manifold and GeneJET Plasmid Midiprep Kit (ThermoFisher) were used for higher yields.
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488/555/647 anti-rabbit IgG, anti-mouse IgG1, anti-mouse IgG2a, anti-mouse IgG2b, or anti-goat IgG; 1:1,000, Invitrogen, Grand Island, NY, USA). Nuclei were counterstained with DAPI (#62248 ...
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bioRxiv - Developmental Biology 2020Quote: ... The following day cells were co-transfected with 2 µg of Fluc plasmid DNA and 20 ng of Rluc control plasmid (pRL) per well using Lipofectamine 2000 (Thermo Fisher, 116688-019) and incubated for 16 h at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T/17 cells at sub-confluence in 6-well plates or 100 mm dishes were transfected with HLA-DRA plasmid or HLA-DRB1 plasmid or a 1:1 combination of both using the Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, UK) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...
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bioRxiv - Cell Biology 2021Quote: The human MISP sequence harbored in a pCMV-SPORT plasmid (Harvard PlasmID Database; HsCD00326629) was subcloned by PCR and TOPOcloned into a pCR™8 Gateway entry vector (Invitrogen; 46-0899). In-frame sequence insertion was confirmed by sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... For lentivirus particle production these plasmids were recombined with pCDH-EF1a-Gateway-IRES-BLAST plasmids as described in [51] using LR Clonase II (Invitrogen/Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... LINC000881 overexpression was achieved by lipofectamine based transfection of LINC00881 plasmid versus bacterial alkaline phosphatase control plasmid using RNAiMAX reagent (ThermoFisher Scientific, Waltham, MA). iPSCs were harvested at 48-72 hours following transfection for functional and gene expression studies ...
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bioRxiv - Microbiology 2021Quote: ... jejuni PglB was amplified by PCR from the plasmid pACYCpgl with the pTac promoter and LacIq repressor from plasmid pEXT20 as a template using Pfx Polymerase (Thermo Fisher Scientific UK) with (SEQ ID 15 ...
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bioRxiv - Immunology 2022Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (ThermoFisher Cat No. 12347019). On day 6 cell culture supernatants were harvested by centrifugation of the cell culture for 30 min at 3500 rpm ...
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bioRxiv - Molecular Biology 2019Quote: To generate linear plasmids without telomeric ends, the circular plasmids were amplified with primers (BP1204, BP1205; Table 2) using Phusion polymerase (Thermo Fisher Scientific, USA). Cycling conditions were ...
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bioRxiv - Synthetic Biology 2022Quote: ... we developed a vacuum-based plasmid extraction protocol named “TeVacS” on Fluent using the PureLink™ Pro Quick96 plasmid purification Kit (K211004A) (Invitrogen, Carlsbad, CA). Our customized protocol features the use of vacuum manifold for efficient removal of washing buffer and final elution of plasmid DNA into a 96-well plate placed on a 3D-printed holder ...
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bioRxiv - Developmental Biology 2022Quote: ... 90 ng of ASD library plasmids and 30 ng of control library plasmids were used for electroporation of MegaX DH10B T1R Electrocomp™ Cells (Thermofisher, cat.no. C640003) following the manufacturer’s guide ...
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bioRxiv - Physiology 2022Quote: ... HPAECs were transfected with either control plasmids (empty vector) or 1μg of HA-ubiquitin plasmids (HA-tagged Ubi) using Lipofectamine 3000 (Thermo fisher Scientific, PA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... a TMPRSS2 plasmid and S plasmids from SARS-CoV-2 variants into 293T cells using Lipofectamine 3000 transfection reagent (L3000-001, ThermoFisher Scientific, Asheville, NC) 39,40 ...
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bioRxiv - Physiology 2022Quote: Human TRPM6 was either transfected in a bicistronic vector pCINeo/IRES-GFP plasmid or in a pcDNA pDEST53 plasmid (ThermoFisher Scientific, Waltham, MA) (26 ...
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bioRxiv - Microbiology 2022Quote: ... we isolated fresh plasmid DNA for use in transfection experiments by using the PureLinkTM HiPure Plasmid Maxiprep Kit (Invitrogen, Thermo Fisher Scientific Corp.). This method minimizes lipopolysaccharide contamination in plasmid DNA extracted from Escherichia coli and reduces non-specific PRR pathway activation in plasmid DNA transfections of mammalian cells ...
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bioRxiv - Microbiology 2022Quote: ... we isolated fresh plasmid DNA for use in transfection experiments by using the PureLinkTM HiPure Plasmid Maxiprep Kit (Invitrogen, Thermo Fisher Scientific Corp.). This method minimizes lipopolysaccharide contamination in plasmid DNA extracted from Escherichia coli and reduces non-specific PRR pathway activation in plasmid DNA transfections of mammalian cells ...
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bioRxiv - Genetics 2024Quote: ... A subset of the saturated culture was used to extract purified plasmid DNA using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, K0503).
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bioRxiv - Biophysics 2022Quote: ... 2 μg total plasmid DNA (725 ng CagFbFP-Q148V plasmid + 1275 mg MTS-CagFbFP plasmid) and 2 μl PLUS Reagent were diluted in 100 μl Opti-MEM (Thermo Fisher Scientific, USA), and 4 μl Lipofectamine LTX were diluted in 100 μl Opti-MEM ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were transfected by transfer plasmid and packaging plasmids psPAX2 (addgene #12260) and pMD2.G (addgene #12259) using Lipofectamine 3000 reagent (Thermo Fisher Scientific # L3000015). Viral particles were concentrated using Lenti-X Concentrator (Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
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bioRxiv - Molecular Biology 2024Quote: ... HEK 293T cells were transiently co-transfected with individual expression plasmids encoding full-length RAI2 and CtBP1 and the reporter plasmid using Lipofectamine 2000 (ThermoFisher Scientific, MA, USA). Co-transfection with the pEGFP-N2 plasmid (Takara Bio ...
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bioRxiv - Neuroscience 2024Quote: ... prior to the expansion of resistant colonies in 100 mL of Luria-Bertani (LB) broth and purification of plasmid DNA using a PureLink HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific; #K210014), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-rabbit or -mouse Alexa 488 and anti-mouse or -rabbit Alexa 568 (Invitrogen) secondary antibodies were used at a 1:250 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Immunohistochemistry for mCherry (mouse, 1:1000, Takara; goat anti-mouse-alexa594, 1:500, Invitrogen) was used to delineate viral vector spread ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the indicated secondary antibodies: goat α-mouse 488 mouse (Invitrogen A11029), goat α-mouse 568 (Invitrogen A11004) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse GSCs and mouse NSCs were fixed with 1% formaldehyde (Thermo Fisher scientific, 28906) for 10 min and quenched with 0.125 M glycine for 5 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-mouse IgG-Alexa 633 1:200 (mouse, A21052, Thermofisher, 1:200).
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bioRxiv - Bioengineering 2023Quote: ... an additional 45-min block was performed in mouse-on-mouse blocking reagent (Invitrogen). Sections were incubated with primary antibodies (Supplementary Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... and corresponding mouse mouse IgG1-PE isotype control (cat# 12-4714-42, ThermoFisher Scientific). Cells were washed twice in FW and analyzed by flow cytometry.
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bioRxiv - Physiology 2023Quote: ... A mouse anti-mouse antibody conjugated with Alexa Fluor 647 was used (Invitrogen A21058). 4′,6′-Diamidino-2-phenylindole staining was used to counterstain cellular nuclei ...
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bioRxiv - Immunology 2024Quote: ... Anti-mouse IL-10 and anti-mouse IL-13 antibodies were purchased from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-Actin (Invitrogen) at 1:3000 in 2% Bovine Serum Albumin (BSA) ...
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bioRxiv - Bioengineering 2020Quote: ... mouse anti-PGK1 monoclonal (Invitrogen)) at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-GAPDH (ThermoFisher AM4300), rabbit anti-Sra-1 (1/1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... or mouse IgG (ThermoFisher, 31903) were added to a spin column containing 20 ml of resin slurry in a 2-ml collection tube ...