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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2020Quote: ... PSG3 5 plasmid was co-transfected into 293T cells along with various HIV envelope plasmids 6 using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific, #11668019) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced by transfection of HEK293T packaging cells with three packaging plasmids (pMDL, pRSV-REV, pCMV-VSVG) [30] and the lentiviral reporter plasmid using Lipofectamine 3000 (Invitrogen, Waltham, MA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... MCF10A cells were co-transfected with 1000 ng of pHrD-IRES-Luc plasmid 16 and 0.1 ng of a CMV-Renilla constitutive internal control plasmid using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015) per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: We cotransfected 10-12 replicates of HCT-116 cells with 5 µg of PB-SRT-Puro plasmid and 5 µg PBase plasmid via Neon electroporation (Thermo Fisher #MPK10025). Each replicate contained 2×106 cells ...
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bioRxiv - Molecular Biology 2020Quote: Both the constructed all-in-one CRISPR plasmids and dual colored KI plasmids were co-transfected into the mESCs using lipofectamine 3000 (Thermo Fisher Scientific). Dissociated mESCs were plated on gelatin-coated 24-well plates with 500 μl of (t)2iL+Y-27632 medium ((t)2iL+Y) ...
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bioRxiv - Microbiology 2020Quote: ... [46] cells were transfected with BZLF1 expression plasmid together with the gB expression plasmid using the Neon Transfection System (Thermo Fisher Scientific) to induce lytic replication ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Purified plasmids were sequenced by Eurofins Genomics.
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bioRxiv - Molecular Biology 2019Quote: ... MCF7 cells were transfected with equimolar amounts of luciferase reporter plasmids and pRL-TK transfection control plasmid with Lipofectamine 3000 (Thermo Fisher Scientific).Luciferase activity was measured 24 h post-transfection by the Dual-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... MCF10A ARPIN KO cells were transfected with the Cas9- and gRNA-containing pX330 plasmid and the donor plasmids described above using Lipofectamine 3000 (Thermo Fisher Scientific). Cells were selected with 0.5 µg/ml of puromycin (InvivoGen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Expi293F cells were transfected at a density of 2 × 106 /ml with 400 ng (pCEP4-myc-ACE2 plasmids) or 500 ng (pCEP4-myc-S plasmids) per ml of culture using ExpiFectamine 293 (Thermo Fisher Scientific) according to the manufacturer’s directions ...
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bioRxiv - Biochemistry 2021Quote: ... A positive clone was selected from Petri-plates and then plasmid from positive clone was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The integrity of cloned gene in the pET28a in terms of orientation and sequence was checked at this stage ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Cells were co-transfected with 800 ng of plasmid of interest and 150 ng plasmid containing EF1a upstream of GFP for normalization with Lipofectamine Stem transfection reagent (Thermo Fisher scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... HEK293Ts cells were transfected with 2nd generation lentiviral packaging plasmids and transfer plasmid using Lipofectamine 3000 transfection reagent (Fisher Scientific cat L3000075). In detail ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded in 6-well plates 24 hours before transfection with ADAR2 expressing plasmids using 400 ng plasmid DNA and Lipofectamine 3000 reagent (Thermo Fisher Scientific) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant T/F LTR U3R-pGL3 reporter plasmid was then purified from a randomly picked colony using GeneJet Plasmid Mini Prep Kit (Invitrogen, Carlsbad, CA) as per the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the entry clones with homologous arms and the pDONR-R4R3-loxP-Hygro-loxP plasmid were recombined into the pGEM-gate plasmid in a three-fragment multisite Gateway recombination (Invitrogen, CA, USA), using an LR II clonase plus reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... CRE-nls-GFP ORF was amplified from addgene plasmid #49056 and inserted into the adenoviral expression vector pAd/CMV/V5-DESTTM Gateway Vector plasmid (Thermo Fisher Scientific). After production and amplification of the adenovirus ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting Gateway entry plasmids were further recombined with the pCDH-EF1a-Gateway-IRES-BLAST destination plasmid using the Gateway LR recombination kit (Invitrogen, cat. #11791020). All cloning vectors were kindly supplied by Dr ...
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bioRxiv - Biochemistry 2024Quote: ... 2 million cells were plated in 10-cm tissue culture plates and co-transfected the next day with 475 ng of plasmid encoding bxb1 recombinase and 7125 ng of the plasmid library using Lipofectamine 3000 (Invitrogen, Carlsbad, CA). Cells were grown at 33 °C for 4 days after transfection ...
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bioRxiv - Microbiology 2024Quote: ... The pEcgRNA-guide plasmids from an overnight culture of Stellar cells expressing the plasmids grown in LB medium with spectinomycin (50 µg/ml) were extracted using GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The pEcgRNA-guide plasmids were individually cloned into LT2c ΔStyLTI competent cells harboring the pEcCas plasmid and plated on both spectinomycin for selection of pEcgRNA (50 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... For endogenous RNA trans-splicing experiments 400 ng of dCas13 expression plasmid was mixed with 400 ng of rcRNA expression plasmid 10ul R Buffer (Thermo Fisher Scientific). For experiments where only dCas13 expression plasmid or rcRNA plasmid was transfected ...
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bioRxiv - Cancer Biology 2023Quote: The NF-κB firefly luciferase reporter plasmid (Bayotime, Shanghai, China) and renilla luciferase reporter plasmid were co-transfected into 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). Firefly and Renilla luciferase activities were measured using the Dual-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... Colonies with confirmed presence of the correct insert by PCR were grown overnight in liquid LB with the appropriate antibiotics at 37°C and plasmids were isolated (GeneJET Plasmid Miniprep Kit, Thermo Fisher Scientific) and verified by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... the gRNA-expressing plasmid (pC016 series, 1200 ng), and the luciferase reporter plasmid (psiCHECK2-PTGES3, 20 ng) using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Positive transformants were inoculated in 5 mL liquid LB medium (omitting the Bacto agar) and plasmids were isolated using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Correct plasmid structure was confirmed using restriction analysis using the FastDigest KpnI enzyme (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Expi293F cells at 2 × 106 cells per ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV empty plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). Twenty-four hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: The injected HDR template (white-gRNA6 gRNA-only gene drive) plasmid was prepared using PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Invitrogen, Cat. #A31231), aliquoted based on the concentrations used for the injections ...
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bioRxiv - Physiology 2023Quote: ... 100 ng pMD2 and 400 ng of each sgRNA CRISPR Cas9 lentivirus plasmid (plasmid amount rate 3:1:4) using Turbofect transfection reagent (Thermo Fisher Scientific). Lentiviral media was centrifuged once at 1500 x g for 3 minutes and the supernatant was collected ...
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bioRxiv - Microbiology 2023Quote: Individual plasmids or combinations of (sub)species-specific gH and gL expression plasmids were transfected into FreeStyle 293-F cells (Thermo Fisher Scientific) using polyethylenimine (PolySciences) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated and after 24 h co-transfected with 50 nM siRNA and 500 ng plasmid DNA per well (same A/D plasmid ratio as described above) using 4 μl Lipofectamine 2000 (#11668019, Thermo Fisher Scientific) in Opti-MEM medium (#31985062 ...
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bioRxiv - Molecular Biology 2023Quote: The newly constructed pcDNA5-FRT-HaloTag7-Flag and pcDNA5-FRT-Rpn13(1–128)-HTP-FLAG-FLAG plasmids were co-transfected with the pOG44 plasmid (Invitrogen #V6005-20) containing Flp recombinase into Flp-In 293 cells (Invitrogen R750-07 ...
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bioRxiv - Microbiology 2023Quote: ... Expi293F cells at 2 × 106 cells per ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV as a junk plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). At 24 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Combinations of plasmids were co-transfected into HEK293T cells (3 µg plasmid, per 10 cm dish) using Lipofectamine 2000 (Thermo Fisher Scientific). 24 hrs post-incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T packaging cells were transfected with the CAR expressing plasmids (GV401-CD19-CAR) together with lentiviral packaging plasmid using Lipofectamine Reagent (Thermo Fisher Scientific). After 48 h ...
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bioRxiv - Genetics 2023Quote: ... capsid plasmid (15 μg per dish) and cargo plasmid (9 μg per dish) were mixed with serum-free DMEM (Thermo Fisher Scientific) and polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with psPAX2 (addgene plasmid #12260) and pMD2.G (addgene plasmid #12259) into HEK 293T cells using TurboFect (Thermo Fisher Scientific). Viral supernatant was collected on day 3 and 4 after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... The cloned lentiviral CRISPR plasmids were verified by Sanger sequencing and co-transfected with LV-MAX packaging plasmids mix (Thermo Fisher, #A43237) into 293T cells using Lipofectamine 3000 transfection reagent (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Spartin plasmid together with psPAX2 and VSV-G plasmid were transfected into HEK293 cells using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) for generating lentivirus ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were picked and cultured overnight in LB medium + 50 µg/mL kanamycin and recombinant plasmids isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher #K0503). Insert sequences in each plasmid were confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: GECs were transfected with 1 µg of mCherry-eGFP-LC3C construct plasmid or a control plasmid (empty vector without LC3C) for 48 h using Lipofectamine 3000 (Thermofisher Scientific, L3000001) in serum free KGM medium as per the manufacturer’s instructions.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Cultured cells were then scaled up to 500 mL and plasmids were isolated using the PureLink™ HiPure Plasmid Maxiprep Kit (ThermoFisher #K210006). The isolated plasmids were filter-sterilized prior to transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were grown in 10 mL LB for 2-3 h and plasmids extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). From each library ...
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bioRxiv - Cancer Biology 2024Quote: ... 6 million HEK 293FT cells were seeded and transfected after 24 hours with the LentiCRISPRV2-IL11g1 or LentiCRISPRV2-IL11g2 construct together with the pLp Δ8,9 packaging plasmid and VSVG envelope plasmid using lipofectamine™ 2000 transfection reagent (Life Technologies, 11668019) and Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Developmental Biology 2019Quote: ... stained with fluorophore-labeled secondary antibodies (Alexa 488 Donkey anti-Mouse, Alexa 555 Donkey anti-Mouse, Alexa 647 Donkey anti-Mouse, Alexa 488 Goat anti-Rabbit; Invitrogen, Carlsbad, CA) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405, Invitrogen #A-31553), tryptophan hydroxylase 2 (primary ...
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bioRxiv - Cancer Biology 2021Quote: Transient plasmid transfection was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected using Lipofectamine 3000 (Life Technologies Invitrogen) following the instructions of the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected using Lipofectamine 3000 (Life Technologies Invitrogen) following the instructions of the manufacturer.