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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... The secondary antibody (Alexa Fluor 594 goat anti-Mouse, ThermoFisher A11032, 1:1000 in 5% GS in PBS) was applied for 1 h at RT in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... 150mM NaCl supplemented with 5% BSA (w/v) and 0.1% NaN3(w/v)): Tim17a (Thermo Scientific, 1:1000), PRELID1 [aa27-54] (Sciquest ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatant was aspirated off and 500 μL of diluted (1:5) TrypLE Express (Cat no. 12604-013, Gibco) was added to each conical to dissociate cell pellet ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were blocked for 1 hr at RT in 5% albumine bovine fraction V (Thermo Scientific, pH 7.0) washed in TBS + 0.05% Tween with pH 8.0 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation for 1 h in blocking solution containing 5% goat serum (16210–064, Thermo Fisher Scientific), 3% bovine serum albumin (BAC62 ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 1 hour at 37°C/5% CO2 with 1μM CellTracker Deep Red (Invitrogen, C34565) and 1 drop/mL NucBlue Live ReadyProbes Reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... the RACE adapter (see Table S2) was ligated to 1-5 µg RNA using T4 RNA ligase (Invitrogen) for 1 hour at 37 °C or 2 hours at 25 °C ...
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bioRxiv - Biophysics 2024Quote: ... which is the same medium as above with 5 μg·ml-1 hygromycin B (Thermo Scientific Chemicals, J60681-MC) replacing the zeocin.
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Immunology 2024Quote: ... after which the cells were resuspended into 1-5 mL FBS and counted on a Countess (ThermoFisher Scientific) with trypan blue ...
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bioRxiv - Immunology 2024Quote: ... 5 x 106 to 1 x 107 cells per sample were lysed by RIPA buffer (Thermo Fisher, 89900) and shaken on a rocker for 1 h at 4°C ...
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bioRxiv - Genomics 2024Quote: ... which included 5 µL of 4x TaqPathTM 1-step RT-qPCR Master Mix (Thermo Fisher Scientific, MD, USA), 1 µL of 10 µM forward primer ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080], 5% FBS [Hyclone SH30070.03HI] and 1% Penn-Strep [Gibco 10378016]). Monoclonal antibody dilution plates were prepared similarly to serum dilution plates ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were diluted in 1xTBST + 5% milk and incubated with membranes for 45 minutes (1:100,000; Invitrogen). Membranes were then washed 3x in 1xTBST washes for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... a tri-gas incubator maintained at 1% O2 was employed (Thermo Scientific, Heracell 150i, 1.0% O2, 5% CO2).
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bioRxiv - Microbiology 2023Quote: ... 1×106 293T cells were seeded prior to transfecting 5 μg of plasmid DNA using Lipofectamine 2000 (Invitrogen), according to manufacturer protocols ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% v/v fetal bovine serum (Eurx, Gdansk, Poland) and 1% penicillin/streptomycin (Thermo Fisher Scientific) at 37°C in a humidified incubator under 5% CO2 and 95% humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies were diluted in 1xTBST + 5% milk and incubated with membranes for 45 minutes (1:100,000; Invitrogen). Membranes were then washed 3x in 1xTBST washes for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 5% CO2 using either DMEM (HeLa and PANC-1: high glucose, GlutaMAX™ Supplement, pyruvate; ThermoFisher Scientific 10569010) or RPMI 1640 (Jurkat and PBMC ...
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bioRxiv - Immunology 2023Quote: ... 5% glycerol and then mixed at 9 volumes to 1 volume of 200× concentrate SYPRO orange (Thermo Fisher) diluted in the same buffer ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 5% (v/v) heat-inactivated fetal bovine serum (FBS) and 1% (v/v) antibiotic-antimycotic (Gibco). HEK293S GnTI- suspension cells (ATCC CRL-3022 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with the same medium with the addition of 5 μg mL−1 zeocin (ThermoFisher Scientific). For neuronal maturation ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin and 10 mM HEPES (pH 7.4)) with 5 µm CellROX Green (Thermo Fisher Scientific, C10444). Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... Both cell lines were cultured in MAT/P (US patent No. 4.816.401) supplemented with 5% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO) and incubated at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... with added 5 mL (1% v/v) P/S (Catalog: 15140-122, Thermo Fisher Scientific, Waltham, MA, USA), 60% Knock-out Serum (Catalog ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of sample were mixed with 2 µL of 1 U/µL DNase I (ThermoFisher Scientific, USA) and 13 µL of 10X DNase I reaction buffer with MgCl2 (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The sample (5 μl of 1 μg/μl) was analyzed in a QExactive-Orbitrap mass spectrometer (Thermo Scientific) using data-independent acquisition (DIA) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with with 5% human platelet lysate (hPL, Stemmatters, Portugal) and 1% Penicillin-Streptomycin (P/S, Invitrogen USA). ASCs were maintained at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... in the presence of 5 ml of ‘neural induction medium’ containing DMEM/F12 supplemented with GlutaMax (1/1; Thermo-Fisher Scientific; Cat. No. 10565-018), Neurobasal medium (1/1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Prepared 200X dilutions were then diluted to 2X concentration in infection media (Gibco DMEM supplemented with 5% HyClone FetalCloneII, 1% Gibco NEAA, 1% Gibco Pen-Strep). Growth media was removed and cells were pretreated with 2 X drug for 1 hour prior to infection at 37C and 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The slides were washed thrice with PBS and blocked with 5 % milk inside a humid chamber for 1 h before being incubated with α-V5 mouse primary antibody (Thermo Fisher Scientific – 1:400) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were then washed 3 x 5 min in PBS and incubated with appropriate secondary antibodies for 1 hr at room temperature (1:1000, ThermoFisher Alexa-Fluor 488, 568, 647). After mounting coverslips onto microscope slides with ProLong gold mounting media (ThermoFisher) ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
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bioRxiv - Neuroscience 2020Quote: Samples (5–10 µg protein) were typically run on 4–12% Bis-Tris gels (Life Technologies; WG1403BOX10) using MOPS buffer (NP0001 ...
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bioRxiv - Bioengineering 2020Quote: Before staining with either 5-bromo-4-chloro-3’-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, ThermoFisher) or Alizarin Red S (ARS ...
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bioRxiv - Cell Biology 2021Quote: ... boiled for 5-10 min before the loading on 4-12 % Bis-Tris gels (Nu-PAGE, Invitrogen) and then transferred onto nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... Exon 4 and Exon 5 were amplified (Table M1) and cloned using pJET1.2 cloning kit (Thermo Fisher) before sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... L-glutamine (5 mg/ml) in the presence 4 μg/ml Blasticidin S HCl (A11139-03, Invitrogen) and 35 μg/ml Zeozin (R250-01 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... centrifuged at 14,000 RPM for 5 min at 4°C and resuspended in UltraPure H2O (Ambion, UK). Purified transcripts were capped using the ScriptCap™ Cap 1 Capping System Kit (CellScript ...
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bioRxiv - Immunology 2020Quote: ... Lipid droplets and nuclei were stained with 0.2% Triton-X100/PBS containing 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503, 5 μg/ml; Thermo Fisher)88 ...
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bioRxiv - Microbiology 2022Quote: ... blotted for 4-5 s and plunge-frozen into liquid ethane in a Vitrobot Mark IV (ThermoFisher), while the blotting chamber was maintained at 100% humidity at 10 ºC ...
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bioRxiv - Immunology 2022Quote: ... centrifugation (table top max speed; 5 mins) before they were run on 4-12% polyacrylamide gels (Invitrogen). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... the equivalent of 5-10 adult worms was loaded into 4-12% NuPAGE Bis-Tris gels (Invitrogen). Proteins were transferred overnight to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2022Quote: ... with a 5 eV slit width and a Falcon 4 or Falcon 4i direct electron detector (ThermoFisher). Images were collected at a pixel size of 0.745 Å/pix or 0.951Å/pix with a cumulative dose of 40e−/Å2 for untilted ...
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bioRxiv - Molecular Biology 2022Quote: Arabidopsis seedlings of 5 days after germination (DAG) were fixed in 4% methanol-free formaldehyde (Thermo Scientific) diluted in nuclease free 1 x PBS (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... After 4-5 h bacteria were collected by centrifugation (5000 rpm, SLA 3000 rotor, ThermoFisher Cat#07149) for 15 minutes and resuspended by gentle trituration in ice-cold 50 ml of TBS Buffer (150 mM NaCl ...