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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... All samples were then incubated with nucgreen™ Dead 488 (Invitrogen™, 1 drop/5 ml in PBS) overnight for spheroids and 4 h for cell monolayers ...
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bioRxiv - Bioengineering 2020Quote: ... and fusion medium (low-serum medium - 94 % Dulbecco’s Modified Eagle’s Medium, Gibco, 5 % horse serum, 1 % antibiotic/ antimycotic Gibco) were changed every other day ...
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bioRxiv - Biophysics 2021Quote: ... Desiccated samples were subjected to 5-6 rounds of desiccation and rehydration (1-hour speedvac desiccation [Thermo Fisher Scientific] followed by resuspension in water) ...
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bioRxiv - Immunology 2020Quote: ... Tissue sections were then stained with DAPI (Thermo Fisher Scientific, USA, 1:5000, 5 min at 37°C) with subsequent mounting with Prolong Diamond Antifade mountant (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Samples of RNA from SKM-1 and MS-5 co-cultures were collected and extracted using Trizol (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µl of EZ-Link™ Sulfo-NHS-Biotin (21217, Thermo Fisher Scientific, 1 µg/20 µl), while still attached via the umbilical cord to the mother’s blood circulation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... + 5% BCS + 0.4 ug/ml hydrocortisone + 10 ng/ml epidermal growth factor (EGF) + 1% Penicillin-Streptomycin (ThermoFisher Scientific). After two days incubation ...
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bioRxiv - Genetics 2022Quote: 5 µL of RT-qPCR reaction was digested with 1 µL FastDigest PstI restriction enzyme (#FD0614, ThermoFisher Scientific) for 1 h at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... an additional cleaning step with acid phenol was added before precipitation of the RNA by adding an equal volume of Acid-Phenol:Chloroform 5:1 solution pH 4.5 (Ambion). Total RNA was quantified with a Nanodrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... to stain AAV2/5-GfaABC1D::eGFP and Alexa 488 anti-chicken (1:400, Thermo Fisher Scientific, The Netherlands). Finally ...
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bioRxiv - Biochemistry 2020Quote: THP-1 cells (ATCC, TIB-202) were cultured at 37°C with 5% CO2 in RPMI 1640 (Invitrogen) supplemented with 10% heat inactivated FBS (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Between 1 and 5 μg of RNA was boiled at 95 °C with Gel II Loading Buffer (Invitrogen) and loaded on a urea-PAGE gel (Sequagel ...
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bioRxiv - Immunology 2019Quote: ... An eight-point dilution series of patient serum diluted at 1:5 in DMEM (Gibco, Life Technologies, USA) supplemented with 2% FBS were incubated with a constant amount of DENV1 - West Pac 74 and DENV-2 S16803 ...
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bioRxiv - Immunology 2019Quote: ... An eight-point dilution series of patient serum diluted at 1:5 in DMEM (Gibco, Life Technologies, USA) supplemented with 2% FBS were incubated with a constant amount of DENV1 - West Pac 74 and DENV-2 S16803 ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes, and fixed with methanol:glacial acetic acid, (5:1, v/v) (FisherThermo Fisher Scientific, Rockford, IL). Cells were dropped onto slides ...
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bioRxiv - Genetics 2020Quote: ... and in some cases day 5 (120 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1-3h at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific). Cells were washed in 1x PBS and fixed in 10% neutral buffered formalin (HT501128 ...
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bioRxiv - Physiology 2021Quote: ... at a concentration of 1:500 for 5 min and mounted in SlowFade Diamond Antifade Mountant (ThermoFisher, S36967). Images were captured using a Leica TCS SP5 Confocal Microscope and processed using Fiji (ImageJ ...
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bioRxiv - Biochemistry 2021Quote: ... and mouse anti-UBE2J1 (1:250) diluted in blocking solution with 0.1% saponin and 5% goat serum (Gibco) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The antibodies used for IF staining were anti-Pax7 (DSHB, cat# PAX7, RRID:AB_528428; 1/5 dilution; or Invitrogen, cat# PA1-117 ...
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bioRxiv - Immunology 2020Quote: ... Splenocytes resuspended at a density of 5×106 cells/ml in PBS had 1:5,000 CFSE (ThermoFisher Scientific) added and were incubated in the dark at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 80 ± 5 mg of brain tissue samples were lysed in 1 mL of TRIzol™ Reagent (Thermo Fisher), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Vero-E6 cells and Calu-3 were treated with 5 μg/ml or 1 μg/ml puromycin (Gibco), respectively ...
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bioRxiv - Pathology 2022Quote: ... The secondary antibody (Alexa Fluor 594 goat anti-Mouse, ThermoFisher A11032, 1:1000 in 5% GS in PBS) was applied for 1 h at RT in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... 150mM NaCl supplemented with 5% BSA (w/v) and 0.1% NaN3(w/v)): Tim17a (Thermo Scientific, 1:1000), PRELID1 [aa27-54] (Sciquest ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatant was aspirated off and 500 μL of diluted (1:5) TrypLE Express (Cat no. 12604-013, Gibco) was added to each conical to dissociate cell pellet ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were blocked for 1 hr at RT in 5% albumine bovine fraction V (Thermo Scientific, pH 7.0) washed in TBS + 0.05% Tween with pH 8.0 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation for 1 h in blocking solution containing 5% goat serum (16210–064, Thermo Fisher Scientific), 3% bovine serum albumin (BAC62 ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 1 hour at 37°C/5% CO2 with 1μM CellTracker Deep Red (Invitrogen, C34565) and 1 drop/mL NucBlue Live ReadyProbes Reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... the RACE adapter (see Table S2) was ligated to 1-5 µg RNA using T4 RNA ligase (Invitrogen) for 1 hour at 37 °C or 2 hours at 25 °C ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% v/v fetal bovine serum (Eurx, Gdansk, Poland) and 1% penicillin/streptomycin (Thermo Fisher Scientific) at 37°C in a humidified incubator under 5% CO2 and 95% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were diluted in 1xTBST + 5% milk and incubated with membranes for 45 minutes (1:100,000; Invitrogen). Membranes were then washed 3x in 1xTBST washes for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... a tri-gas incubator maintained at 1% O2 was employed (Thermo Scientific, Heracell 150i, 1.0% O2, 5% CO2).
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bioRxiv - Microbiology 2023Quote: ... 1×106 293T cells were seeded prior to transfecting 5 μg of plasmid DNA using Lipofectamine 2000 (Invitrogen), according to manufacturer protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies were diluted in 1xTBST + 5% milk and incubated with membranes for 45 minutes (1:100,000; Invitrogen). Membranes were then washed 3x in 1xTBST washes for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... 5% glycerol and then mixed at 9 volumes to 1 volume of 200× concentrate SYPRO orange (Thermo Fisher) diluted in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 5% CO2 using either DMEM (HeLa and PANC-1: high glucose, GlutaMAX™ Supplement, pyruvate; ThermoFisher Scientific 10569010) or RPMI 1640 (Jurkat and PBMC ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with the same medium with the addition of 5 μg mL−1 zeocin (ThermoFisher Scientific). For neuronal maturation ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 5% (v/v) heat-inactivated fetal bovine serum (FBS) and 1% (v/v) antibiotic-antimycotic (Gibco). HEK293S GnTI- suspension cells (ATCC CRL-3022 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin and 10 mM HEPES (pH 7.4)) with 5 µm CellROX Green (Thermo Fisher Scientific, C10444). Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2024Quote: ... 5 x 106 to 1 x 107 cells per sample were lysed by RIPA buffer (Thermo Fisher, 89900) and shaken on a rocker for 1 h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... after which the cells were resuspended into 1-5 mL FBS and counted on a Countess (ThermoFisher Scientific) with trypan blue ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080], 5% FBS [Hyclone SH30070.03HI] and 1% Penn-Strep [Gibco 10378016]). Monoclonal antibody dilution plates were prepared similarly to serum dilution plates ...
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bioRxiv - Cancer Biology 2023Quote: ... Both cell lines were cultured in MAT/P (US patent No. 4.816.401) supplemented with 5% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (GIBCO) and incubated at 37°C ...
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bioRxiv - Genomics 2024Quote: ... which included 5 µL of 4x TaqPathTM 1-step RT-qPCR Master Mix (Thermo Fisher Scientific, MD, USA), 1 µL of 10 µM forward primer ...
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bioRxiv - Biophysics 2024Quote: ... which is the same medium as above with 5 μg·ml-1 hygromycin B (Thermo Scientific Chemicals, J60681-MC) replacing the zeocin.
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Microbiology 2022Quote: ... The F gene was PCR amplified using primers RSV F 5’ (5’GCAAGGATTCCTTCGTGAC3’) and RSV F 3’ (5’CACACCACGCCAGTAG3’) and Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific). Sanger sequencing was performed by Elim Biosciences.
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bioRxiv - Clinical Trials 2019Quote: ... (Bruner and Siliciano, 2018), env (Forward: 5’-AGTGGTGCAGAGAGAAAAAAGAGC-3’, Reverse: 5’-GTCTGGCCTGTACCGTCAGC-3’, Probe: 5’/VIC/CCTTGGGTTCTTGGGA/3’/MGB) (Thermo Fisher Scientific) (Bruner et al. ...