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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Counterstaining was with 4’-6-diamidino-2-phenylindole (DAPI) (Invitrogen) at 20 µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... DAPI (4’,6-Diamidin-2-phenylindol, Dihydrochloride; Thermo Fisher Scientific) staining was performed prior to recording ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher D1306) and stored in the dark at 4°C until imaging.
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bioRxiv - Cell Biology 2023Quote: ... on QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). The PCR protocol started with one cycle at 95°C (10 min ...
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bioRxiv - Microbiology 2023Quote: ... and centrifugation with a Sorvall HB-6 rotor (Thermo Fisher) at 5500 rpm (4900xg ...
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bioRxiv - Molecular Biology 2023Quote: ... for 6 hours in less serum optiMEM media (Thermofisher, cat31985062). After 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 6 gram of 5% G-250 NativePAGE Sample additive (Invitrogen) was added per gram of mitochondria-enriched fraction ...
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bioRxiv - Developmental Biology 2024Quote: ... and the medium was replaced with Essential 6 medium (Gibco) containing VEGF-A (80 ng/mL) ...
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bioRxiv - Developmental Biology 2024Quote: ... on a QuantStudio 6 Flex Real-Time PCR unit (ThermoFisher). Changes in RUNX1 expression were calculated using the 2-ΔΔCT methodology.
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bioRxiv - Genetics 2024Quote: ... and DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific). For Figure 5A and C and Figure 6 ...
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bioRxiv - Developmental Biology 2024Quote: ... and the QuantStudio 6 Flex Real-Time PCR System (ThermoFisher). RpL32 was used as an internal control ...
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bioRxiv - Genetics 2024Quote: ... and DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific). For Figure S7 ...
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bioRxiv - Biochemistry 2024Quote: ... mixture for 6 hrs in optiMEM (Gibco; reduced serum media) followed by addition of 10% FBS containing DMEM/F12 media for each round of transfection ...
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bioRxiv - Genomics 2024Quote: ... and then examined in the QuantStudio 6 Flex system (ThermoFisher). Relative gene expressions were normalized against the internal expression of GAPDH ...
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bioRxiv - Neuroscience 2024Quote: ... with readout on a QuantStudio 6 Flex System (Thermo Scientific) using standard cycling parameters ...
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bioRxiv - Cell Biology 2024Quote: ... coated 6 well plates and maintained in StemFlex (Thermo Scientific) media at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-acetylated tubulin (clone 6-11B, 32270, Thermo Fisher) at 1:10000 ...
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bioRxiv - Cell Biology 2024Quote: ... A human IL-6 ELISA kit (Thermo Fisher Scientific, KHC0061) was then used as instructed by the manufacturer to measure IL-6 concentrations ...
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bioRxiv - Neuroscience 2024Quote: ... cells were switched to Essential 6 medium (Thermo Fisher Scientific) supplemented with 0.5 μM of LDN193189 (BMP inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... incubated with DAPI (40, 6-diamidino-2-phenylindole, Invitrogen, D1306) (0.5mg/ml in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... on QuantStudio 6 Flex Real-Time PCR System (Life Technologies). 18S rRNA (ribosomal RNA ...
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bioRxiv - Neuroscience 2024Quote: ... coated 6-well dish in E8 medium (cat #A1517001, ThermoFisher) supplemented with 10 uM Y-27632 (Y27 ...
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bioRxiv - Developmental Biology 2024Quote: ... 6 units of SUPERase•In™ RNase Inhibitor (Invitrogen, AM2694) were added to the antibody mix ...
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bioRxiv - Microbiology 2024Quote: ... Reactions were run on a QuantStudio 6 pro (Thermo Fisher). Relative abundance was normalized by ΔΔCt.
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bioRxiv - Microbiology 2024Quote: ... and stained with DAPI (4′,6-diamidino-2-phenylindole) (Invitrogen). This process ...
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bioRxiv - Neuroscience 2024Quote: ... 320 mOsm/kg) with 6 µM Fura-2AM (F1221, ThermoFisher), and 0.04% Pluronic F-127 (P2443 ...
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bioRxiv - Bioengineering 2021Quote: HEK293FT cells were seeded into 6-well plates at 1 million cells/well and transfected using Lipofectamine 2000 (Life Technologies, Grand Island, NY) according to instructions at sub-confluence ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody amplification was done in 6-mL 1:600 (10 μl) Alexa Fluor 568 donkey anti-rabbit IgG (Thermo Fisher, Lot# 2044343) for 6 days.
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bioRxiv - Biochemistry 2020Quote: ... Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008) as per manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (as above) in 6-well plates (Nunc, cat. No. 140675) and acclimated for 2 days with mild agitation (130 rpm) ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells plated in 6-well plates the previous day were transfected with three plasmids (1 ug each per well) using Lipofectamine 2000 (Thermo Fisher, Waltham, MA). Following overnight incubation ...
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bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular pH of sea urchin embryos was visualized using pHrodo Red AM intracellular pH indicator or 5-(and-6)-carboxy SNARF-1 (C-1270) (Thermo Fisher Scientific, USA) at final concentrations of 10 and 5 μM ...
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bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells cultured overnight (1×105 cells/well in 6-well plates) were transfected with plasmid and 5μl Lipofectamine 2000 (Thermo Fisher Scientific, United States). The cells were then incubated at 37 °C for 24 h before analysis.
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 6×10 minutes and incubated with an AlexaFluor488-conjugated goat-anti-guinea pig IgG (1:200, Invitrogen A-11073, RRID:AB_2534117) for 4 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Western blots were performed under reducing conditions as previously detailed (6) to probe for PEMT (dilution 1:1000, Thermo Fisher Scientific, PA5-42383) and loading control GAPDH (dilution 1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... On day 6, the medium was changed to neural induction medium (NIM, DMEM/F12 GlutaMAX supplemented with 1:100 N2 supplement (Gibco™, 15502-048), 1 % Non-essential amino acids and 1 µg/ml Heparin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed twice by resuspending in 1 mL 1% bovine serum albumin (BSA; Bovagen, NZ) in PBS and centrifugation for 1 minute in a mySPIN™6 mini centrifuge (Thermo Scientific, USA). The resulting pellet was permeabilized for 10 min at room temperature in 0.1% Triton®X-100 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
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bioRxiv - Neuroscience 2024Quote: ... and plated again in a dilution 1:6 in neural differentiation medium to induce neuroepithelial differentiation (NDM; 50% DMEM-F12 (Gibco, Grand Island, NY), 50% Neurobasal (Gibco ...
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bioRxiv - Pathology 2023Quote: Total DNA was then extracted from HIV-1 infected cells at 6 h post-infection using the PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific). Quantitative PCR for R-U5 and LTR-Gag transcripts was performed using the following primers:
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bioRxiv - Developmental Biology 2019Quote: ... FACS-purified CD31+CD146+ primed vs naïve VP populations were expanded in EGM2 medium ∼7 days to 60 to 70% confluency on fibronectin pre-coated 6-well plates (1-1.5 x 105 cells/well) prior to Dil-Ac-LDL uptake assays (Life Technologies, Cat No. L-3484). Fresh EGM2 medium supplemented with 10 μg/mL Dil-Ac-LDL ...
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Microbiology 2022Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:3000; Thermo Fisher, HYB-076-08-02), rabbit anti- CFP-10 (1:5000 ...