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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: Mouse IL-6 Uncoated ELISA kit (cat# 88-7064; Invitrogen) was used to measure Interleukin-6 concentrations of mouse tissue lysates ...
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bioRxiv - Genomics 2024Quote: ... analysed in QuantStudio 6 Flex instrument (Applied Biosystems, ref. 4485691). RNA levels were normalised to endogenous HPRT using ΔΔCt method ...
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bioRxiv - Microbiology 2024Quote: ... on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). Primers used for analysis are as follows ...
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bioRxiv - Microbiology 2024Quote: Novex 6% TBE-urea gels in 1x TBE buffer (Invitrogen) were pre-run at 180 V for at least 50 minutes prior to sample loading ...
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bioRxiv - Microbiology 2024Quote: ... and QuantStudio 6 Flex real-time PCR System (ThermoFisher Scientific). qPCR began with an initial denaturation phase at 95°C for 20 s ...
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bioRxiv - Bioengineering 2021Quote: HEK293FT cells were seeded into 6-well plates at 1 million cells/well and transfected using Lipofectamine 2000 (Life Technologies, Grand Island, NY) according to instructions at sub-confluence ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody amplification was done in 6-mL 1:600 (10 μl) Alexa Fluor 568 donkey anti-rabbit IgG (Thermo Fisher, Lot# 2044343) for 6 days.
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bioRxiv - Biochemistry 2020Quote: ... Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008) as per manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (as above) in 6-well plates (Nunc, cat. No. 140675) and acclimated for 2 days with mild agitation (130 rpm) ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection complex and BxPC3 cells and PNAC-1 cells were then mixed together and seeded in 6-well (2 x 105 cells/well) cell culture plates (Nunc, Rochester, NY, USA) and cultured at 37°C for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells plated in 6-well plates the previous day were transfected with three plasmids (1 ug each per well) using Lipofectamine 2000 (Thermo Fisher, Waltham, MA). Following overnight incubation ...
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bioRxiv - Immunology 2022Quote: PECs were harvested from untreated wild-type B6 and Nr4a1-/- mice and allowed to adhere to plastic wells (6-well plates, 2 × 106 cells/well) for 1 hour in AIM V serum free medium (ThermoFisher Scientific, Waltham, MA). Non-adherent cells were washed off with PBS and the adherent cells were cultured for 3-hrs in complete DMEM + 10% FBS medium containing LPS (1 μg/ml in PBS ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular pH of sea urchin embryos was visualized using pHrodo Red AM intracellular pH indicator or 5-(and-6)-carboxy SNARF-1 (C-1270) (Thermo Fisher Scientific, USA) at final concentrations of 10 and 5 μM ...
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bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells cultured overnight (1×105 cells/well in 6-well plates) were transfected with plasmid and 5μl Lipofectamine 2000 (Thermo Fisher Scientific, United States). The cells were then incubated at 37 °C for 24 h before analysis.
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 6×10 minutes and incubated with an AlexaFluor488-conjugated goat-anti-guinea pig IgG (1:200, Invitrogen A-11073, RRID:AB_2534117) for 4 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Western blots were performed under reducing conditions as previously detailed (6) to probe for PEMT (dilution 1:1000, Thermo Fisher Scientific, PA5-42383) and loading control GAPDH (dilution 1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed twice by resuspending in 1 mL 1% bovine serum albumin (BSA; Bovagen, NZ) in PBS and centrifugation for 1 minute in a mySPIN™6 mini centrifuge (Thermo Scientific, USA). The resulting pellet was permeabilized for 10 min at room temperature in 0.1% Triton®X-100 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
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bioRxiv - Neuroscience 2024Quote: ... and plated again in a dilution 1:6 in neural differentiation medium to induce neuroepithelial differentiation (NDM; 50% DMEM-F12 (Gibco, Grand Island, NY), 50% Neurobasal (Gibco ...
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bioRxiv - Pathology 2023Quote: Total DNA was then extracted from HIV-1 infected cells at 6 h post-infection using the PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific). Quantitative PCR for R-U5 and LTR-Gag transcripts was performed using the following primers:
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bioRxiv - Neuroscience 2024Quote: ... On day 6, the medium was changed to neural induction medium (NIM, DMEM/F12 GlutaMAX supplemented with 1:100 N2 supplement (Gibco™, 15502-048), 1 % Non-essential amino acids and 1 µg/ml Heparin (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... FACS-purified CD31+CD146+ primed vs naïve VP populations were expanded in EGM2 medium ∼7 days to 60 to 70% confluency on fibronectin pre-coated 6-well plates (1-1.5 x 105 cells/well) prior to Dil-Ac-LDL uptake assays (Life Technologies, Cat No. L-3484). Fresh EGM2 medium supplemented with 10 μg/mL Dil-Ac-LDL ...
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bioRxiv - Bioengineering 2021Quote: ... and each lobe was cultured statically in a 6 well plate in cell culture media (DMEM high glucose, 10% FBS, 1% pen-strep) (Thermo Fisher Scientific, Waltham, MA). Media was changed on days 1 ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Microbiology 2022Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:3000; Thermo Fisher, HYB-076-08-02), rabbit anti- CFP-10 (1:5000 ...
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bioRxiv - Developmental Biology 2020Quote: ... (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY® F C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) was purchased from Molecular Probes (Melbourne, VIC, Australia). The TiO2 was collected from a disassembled column ...
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bioRxiv - Microbiology 2019Quote: ... The cells were plated on 6-well plates at 1 × 105 cells/well or onto 4-channel chamber slides (Lab-Tek II chamber slide, Thermo Fisher Scientific, Waltham, MA) at 5 × 104 cells/well and were grown until reaching 70% confluence in serum-free EpiLife medium supplemented with HKGS.
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bioRxiv - Immunology 2021Quote: ... Equal amounts of heavy and light chain expression plasmids and a 1:3 ratio of PEI was used to transfect 293-6E cells at a density of 1×10^6 cells/mL in Freestyle 293 media (Thermo Fisher Scientific, Cat#: 12338018). Supernatants were collected 6 days post transfection by centrifugation at 4,000g followed by filtration through a 0.22 μM filter (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... CFP-10 and GroEL2 were determined by western blotting with mouse anti-ESAT-6 clone 11G4 (1:1,000; Thermo Fisher, HYB-076-08-02), rabbit anti-CFP-10 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 1×106 HL-60 cells were seeded on cell 75 cm2 culture flask in 6 mL IMDM medium (Thermo Fisher Scientific, Waltham, MA, US) and incubated for 24 h in the presence of compound 1 at 10 nM and 10 μM concentrations ...
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bioRxiv - Biophysics 2021Quote: ... Nucleus were stained with DAPI (4’, 6-diamidino-2-phenylindole, Invitrogen) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Resultant peptides were labelled with TMT 6-plex reagents (Thermo Scientific) at an 8:1 tag:protein ratio as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) or a CFXConnect Real-Time PCR Instrument (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was stained with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). After washing with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and run on pre-casted 6% polyacrylamide Novex TBE gel (Invitrogen). Bands of sizes 140-170 bp were selected ...
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bioRxiv - Genomics 2021Quote: ... Co-transfections were performed with 6 μl of Lipofectamine 2000 (Invitrogen) diluted in Opti-MEM (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were resolved on a 6% TBE polyacrylamide gel (ThermoFisher Scientific), size-selected to remove primer-primer ligated DNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 6 μl of trypan blue at 0.4% (Invitrogen, Carlsbad, CA, USA) were added to color the dead cells ...
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bioRxiv - Biochemistry 2022Quote: ... Cells (2 × 105) were plated in 6 well culture dishes (Nunc™ ...
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bioRxiv - Neuroscience 2021Quote: ... The spheroids were then collected and placed into Matrigel (Corning)-coated 6 well-plates with DMEM/F12 (#11330057; Thermo Fisher Scientific), supplemented with 1X N2 (#17502048 ...
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bioRxiv - Neuroscience 2021Quote: ... 6-diamidino-2-phenylindole (DAPI, 0.1 mg/ml, D1306; Molecular Probes) was added into the incubation solution to visualize cell nuclei ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR was performed on a QuantFlex 6 system (Applied Biosystems) using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... and analyzed on QuantStudio 6 Real-Time PCR machine (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were carried out in a Quantstudio 6 instrument (ThermoFisher Scientific), with the following cycling conditions ...
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bioRxiv - Microbiology 2019Quote: ... plated on 6-well Nunclon© plates (Thermo Fisher Scientific, UK). Supernatants were collected 48-72 h post transfection and filtered through a 0.45 μm filter (Millipore ...
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bioRxiv - Developmental Biology 2019Quote: ... 2mL of in Essential-6 media (ThermoFisher Scientific, Waltham, MA, USA) were added into existing 2mL StemSpan medium on MEF in which transfected cells were ...