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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... for 6 hours in less serum optiMEM media (Thermofisher, cat 31985062). Then ...
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bioRxiv - Immunology 2023Quote: ... 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid solution (Invitrogen, 002024) was added to the wells as the coloring substate for HRP ...
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bioRxiv - Microbiology 2023Quote: ... or 6 was then used to transform into stbl3 strain (Invitrogen) for further plasmid amplification.
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bioRxiv - Biophysics 2023Quote: ... using the QuantiStudio 6 Flex system (Applied Biosystems: Waltham, MA, USA). Gene expression was analyzed using the ΔΔCT method ...
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bioRxiv - Physiology 2024Quote: ... in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). The Rplp0 mRNA was used as a loading control ...
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bioRxiv - Bioengineering 2023Quote: ... The 4′,6-diamidino-2-phenylindole (DAPI) was acquired from Invitrogen, Thermofisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, USA), with a 20 µl reaction mixture ...
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bioRxiv - Genetics 2024Quote: ... and the homozygote variant iPSCs were grown in a feeder-free manner on Matrigel (Corning)-coated 6-well plates in Essential 8 (E8) medium (ThermoFisher Scientific). Media was changed daily ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were loaded onto precast 6% TBE (Invitrogen, Fisher cat #EC62655BOX) or 10% TBE-Urea (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... for detection in QuantStudio 6 Pro cycler (Applied Biosystems Carlsbad, CA). Quantification of each transcript was normalized to the mouse 18S reference gene following the 2-ΔΔCt method ((Livak & Schmittgen ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was eluted 6 times in ultrapure water (Thermofisher, 10977) for maximum recovery ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306) was used to stain the DNA content of cells so that doublets and debris could be removed by sorting on the DAPI height vs DAPI area ...
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bioRxiv - Neuroscience 2024Quote: ... were coated with IL-6 capture antibody (ThermoFisher Scientific, MP5-20F3) and left overnight at 4°C ...
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bioRxiv - Genetics 2023Quote: ... and analysed using the Quant Studio 6 Flex system (Applied Biosystems). The real-time qPCR conditions were one hold at (95 °C ...
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bioRxiv - Physiology 2024Quote: ... on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with specific primers for each gene ...
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bioRxiv - Neuroscience 2024Quote: ... or Quant Studio 6 Pro Real-Time PCR System (Applied Biosystems). TaqMan assay details are listed in Tab ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.30 M di-benzo-18-crown-6-ether (Thermo Scientific) following published protocols66,67,79,80,110 ...
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bioRxiv - Cancer Biology 2024Quote: ... The desired DNA product was purified with 6% TBE gel (Invitrogen) and samples were sequenced on an Illumina HiSeq2000 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were grown for 6 passages in DMEM-HAM’s F12 (Gibco) supplemented with 5% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Real-time qPCR was performed on QuantStudio 6 Pro (Applied Biosystems) using iTaq Universal SYBR Green Supermix (Biorad) ...
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bioRxiv - Immunology 2024Quote: ... and human IL-6 (Gibco, PeproTech, #200-06; 20 ng/mL). Prostaglandin E2 (PGE2 ...
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bioRxiv - Microbiology 2024Quote: ... 6-5 cells were maintained in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% FetalPlex (Gemini Bio-Products) ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and mounted on to glass slides in FluorSave™(Millipore) ...
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bioRxiv - Immunology 2024Quote: ... and real-time PCR system (Applied Biosystems 7500 QuantStudio 6 Pro). The Ct value of target genes obtained from each sample was normalized to housekeeping gene GAPDH and relative gene expression was quantified using 2-ΔΔct values.
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bioRxiv - Molecular Biology 2024Quote: ... and eluted in 6 μl nuclease-free water (Ambion, cat# AM9937).
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was diluted 1: 25 and 6 µl was used for one qPCR reaction with PowerUp™ SYBR™ Green Master Mix (Thermo Fisher Scientific, A25776). Relative gene expression was calculated using the ΔΔCT method relative to ACTB control.
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bioRxiv - Plant Biology 2020Quote: Lines with defective stomatal responses to at least one stimulus were subjected to sequencing of “usual suspects” either by NGS-based sequencing of PCR amplicons obtained with the use of gene-specific primers (Supplementary Data Set 2) and Phusion DNA polymerase (ThermoFisher Scientific; ROUND 1 to 6), or whole-genome sequencing (ROUND 7 and 8) ...
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bioRxiv - Microbiology 2021Quote: ... was added to the Lysing Matrix E tube and supplemented with 6 µL of lysozyme (50 mg mL-1, Thermo Fisher Scientific, Cat. no. 90082), followed by a 60 min incubation at 37°C with mixing at 300 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nucleofected cells were then seeded into a 6-well plate in mTeSR™-1 supplemented with Y-27632 (10 μM) and underwent blasticidin (ThermoFisher Scientific; 10 μg/ml) selection for 6 days ...
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bioRxiv - Genetics 2019Quote: ... One μL of diluted cDNA (1:25) was used for real-time PCR in a QuantStuioTM 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, California, USA). Peptidylprolyl isomerase A (PPIA ...
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bioRxiv - Cancer Biology 2023Quote: ... in PBST and stained for 1 hour with primary antibodies (ZEB1: Bethyl, IHC-00419; E-Cadherin: BD Biosciences, 610181; Collagen 6: Abcam, ab182744; αSMA: Thermo Fisher Scientific, 14-9760-82) and for 30 min with secondary Alexa488/647-conjugated antibodies (anti-mouse-/anti-rabbit-IgGs ...
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bioRxiv - Neuroscience 2023Quote: ... Fetal brains were evaluated by Western blot (corticotropin releasing factor receptor 1 [CRFR1]; glucocorticoid receptor [GR], interleukin [IL]-6 receptor [IL-6R]; IL-17A and β-actin, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Plant Biology 2019Quote: ... The mixture was analyzed by immunoblotting anti-6×His (Invitrogen, #MA1-135), anti-MBP (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2021Quote: ... Cell nucleus were stained with DAPI (4’,6-diamidino-2-phenylindole, Invitrogen) for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... were subsequently labeled using 6-plex tandem mass tags (Thermo Fisher Scientific) following the manufacturer’s protocol but employing a reagent to peptide ratio of four ...
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bioRxiv - Cancer Biology 2021Quote: ... qPCR was performed using FastSYBRGreen 5x MasterMix on a QuantStudio 6 (Invitrogen). Analysis was done using the ΔΔCt method using HPRT as housekeeping gene.
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bioRxiv - Cell Biology 2020Quote: ... were seeded in 6-well plates in 1.5 ml DMEM (Life Technologies) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2021Quote: ... cells were grown in 2x YPD media (Fisher Scientific, DF0427-17-6) supplemented with 80 mg/L of adenine hemisulfate ...
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bioRxiv - Neuroscience 2021Quote: ... in the Quant Studio 6 Flex system (Applied Biosystems, Thermo Fisher Scientific). Each sample was analyzed in duplicate ...
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bioRxiv - Neuroscience 2021Quote: ... in the Quant Studio 6 Flex system (Applied Biosystems, Thermo Fisher Scientific). Each sample was analyzed in duplicate ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µl reaction volume was loaded onto 6% DNA retardation gels (Invitrogen), and electrophoresis was carried out at room temperature in 0.5x Tris borate buffer ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... All preparations were stained with 4’,6’-diamidino-2-phenylindole (DAPI, Invitrogen) for 10 minutes to label nuclei and mounted with Fluoroshield medium (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). Transcript levels were analyzed using the delta delta Ct method and GAPDH was used as an internal control (110) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were resolved on a 6 % DNA Retardation gel (Thermo Fisher Scientific) with 0.5x TBE running buffer at 100 V for 60 min at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei were counterstained with 4’,6’-diamidino-2-phenyliondole (DAPI; Invitrogen, D1306). Coverslips were mounted with ProLong™ Gold Antifade (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 30 min incubation with 4′,6-diamidino-2-phenylindole (DAPI, Life Technologies) (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... slides were briefly stained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher) to reveal the nuclei ...
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bioRxiv - Genomics 2020Quote: ... followed by nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen). Both bright field and nuclear staining images were separately taken using a Keyence BZ-X700 All-in-One microscope.