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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The sections were rinsed with PBS (3×10min) and incubated for 6 hours with secondary donkey anti-rabbit 488 antibodies (1:1000, ThermoFisher Scientific, A11006) and streptavidin 594 (1:750 ...
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bioRxiv - Genetics 2022Quote: ... These pieces were transferred to the lower surface of a 25 cm2 culture flask (6-8 pieces per flask) which had been pre-moistened with 1-2 mL of AmnioMAX Complete Medium (Thermo Scientific, #11269016) supplemented with 1% penicillin/streptomycin (GenDepot ...
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bioRxiv - Biophysics 2022Quote: ... 1 x 106 Sf9 cells were transferred to each well of a six-wells plate and subsequently transfected with 1 μg of bacmid DNA by 6 μl of Cell-fectin (Thermo Fisher Scientific). Baculovirus containing cell supernatants (P1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...
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bioRxiv - Immunology 2022Quote: ... Media was removed from THP-1 monocytes cultures (5-6 x 105 cells/ml) and cells were washed once with DPBS (Thermo Fisher Scientifics). A total of 2 x 106 cells were resuspended in 50 μl of BTXpress solution and the Cas9-sgRNA complex was added ...
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bioRxiv - Immunology 2023Quote: ... The pellets were resuspended in 4.5 mL distilled water to which is added 0.5 mL 6% hypochlorite solution (Fisher Scientific Cat# SS290-1) and gently rocked for 1 minute followed by centrifugation (800g for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 6×10 minutes in .05M PBS and incubated with both Alexa-Fluor 488 and 555 secondary antibody (1:1000; Thermo Fisher Scientific) in .05M PBS with .1% Triton x100 for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... was used to amplify the genes of interest according to the primers mentioned in the Table 1 from 15-20 ng of cDNA in the QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific) machine ...
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bioRxiv - Microbiology 2023Quote: ... 10 x 1 µL droplets of each diluted suspension were deposited on polystyrene material (6-well plates, Thermo Scientific, Cat. No. 140675) and exposed to ambient temperature and variable RH (20% ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and placed in a 6-well cell culture plate filled with 1 ml/well of culture media containing 50% Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23% Earle’s Balanced Salt Solution ...
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bioRxiv - Cell Biology 2023Quote: ... 1.7 x 105 Huh7 cells were seeded in a 6-well plate and transfected with 1 µg purified dsRNA mixed with Lipofectamin 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cell Biology 2023Quote: ... 2 x 105 cells were seeded in a 6-well plate containing coverslips and transfected with 1 µg dsRNA mixed with Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... or macroH2A-3Xflag (6 x 106 on 100 mm dish) were cross-linked with 1 % of methanol-free formaldehyde (Thermo Scientific, 28906) in DMEM for 10 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with serum-free RPMI-1640 medium before being transfected with 5 µL of siRNA pool (20 µM) and 5 µL Lipofectamine 2000 in a 6-well plate in 1 mL of antibiotic-free Opti-MEM (Gibco, #31985-062) medium plus 2 mL antibiotic-free cell-culture medium ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 1 µg of the indicated plasmids (6-well plates) by using the Lipofectamine 2000 system (Thermo Fisher Scientific). For transient transfection with siRNA ...
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bioRxiv - Bioengineering 2023Quote: ... PCs were seeded into 6-well plates coated with collagen IV (100 μg/mL) and were cultured in DMEM (1 g/L glucose, Gibco, cat# 11885084) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was extracted from the amygdala of female offspring at P7 and P90 (n=6/mCHD, n=6/mHFD) using TRIzol Reagent (15596018; ThermoFisher Scientific, Ottawa, ON, CAD) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... or 52- or 78-week-old C57BL/6 mice or C57BL/6-TgTM4SF5 mice by perfusion of the liver using collagenase type II (Life Technologies, Carlsbad, CA, USA) (Ateş et al ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were harvested from 6-week-old C57BL/6 mice and were differentiated into dendritic cells using supplemented culture medium: RPMI 1640 (Life Technologies, Waltham, Massachusetts, USA), 10% HIFBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Primary mouse microglia were plated at a density of 3×105 in 6 well plates and treated for 6 h prior to RNA extraction with Trizol (Invitrogen; Taïb et al., 2013). Adult microglia were treated as described in section 2.2.3 prior to extraction of total RNA using RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... primary neurons were plated on 6-well culture dishes (ThermoFisher) at a density of 2 million cells per well and cultured for 7 days prior to drug treatments ...
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bioRxiv - Neuroscience 2021Quote: ... primary neurons were plated on 6-well culture dishes (ThermoFisher) at a density of 2 million cells per well and cultured for 3 days prior to transfection ...
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bioRxiv - Neuroscience 2021Quote: ... primary neurons were plated on 6-well culture dishes (ThermoFisher) at a density of 2 million cells per well and cultured for 7 days prior to drug treatments ...
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bioRxiv - Neuroscience 2021Quote: ... primary neurons were plated on 6-well culture dishes (ThermoFisher) at a density of 2 million cells per well and cultured for 3 days prior to siRNA transfection ...
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bioRxiv - Cell Biology 2020Quote: ... mitochondria isolation kit and IL-6 kit were from Invitrogen and were purchased from ALT Ukraine Ltd (representative of Thermo Fisher Scientific in Ukraine).
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bioRxiv - Cell Biology 2020Quote: ... 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, D1306) was utilized to detect the nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... Human IL-6 and IL-8 ELISAs were from Thermofisher Scientific ...
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bioRxiv - Immunology 2020Quote: Mouse IL-6 was quantified by uncoated ELISA Kit (ThermoFisher) according to manufacturer’s instruction.
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bioRxiv - Evolutionary Biology 2021Quote: ... or 6-9 Locusta eggs per treatment using Trizol (Invitrogen) extraction ...
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bioRxiv - Molecular Biology 2021Quote: ... and 6 and by POROS CaptureSelect™ AAV8 (Thermo Fisher) affinity chromatography as previously described (35).
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bioRxiv - Microbiology 2021Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... and QuantStudio 6 Flex Real-time PCR system (Applied Biosystems) were used ...
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bioRxiv - Physiology 2019Quote: ... pre-validated 6-carboxyfluorescein (FAM)-labeled Taqman probes (Applied Biosystems) against Stim ...
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bioRxiv - Biochemistry 2019Quote: ... selected with 6 μg/mL of Blasticidin S HCl (Gibco) for 7-10 days ...
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bioRxiv - Immunology 2021Quote: ... Thermocycling (QuantStudio 6 Real-Time PCR Flex System; Applied Biosystems) parameters comprised the following ...
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bioRxiv - Immunology 2021Quote: ... Thermocycler (QuantStudio 6 Real-Time PCR Flex System; Applied Biosystems) parameters consist of initial denaturing ...
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bioRxiv - Neuroscience 2020Quote: ... DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, ThermoFisher Scientific, D1306) was applied to nuclei samples at a concentration of 0.1µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) according to instructions of the manufacturer ...
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bioRxiv - Bioengineering 2020Quote: ... RT-qPCR was run on a QuantStudio 6 (Applied Biosystems) with RT at 48 °C for 30 min and 45 cycles with a denaturing step at 95 °C for 10 s followed by annealing and elongation steps at 60 °C for 45 s ...
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bioRxiv - Bioengineering 2020Quote: ... incubated with 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher), rinsed with TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Media was replaced with Essential 6 media (E6, Life Technologies) for 2 consecutive days ...
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bioRxiv - Microbiology 2021Quote: ... using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) according to instructions of the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Mouse ELISA Interleukin (IL)-6 kit was purchased from Invitrogen, Philippines ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 6 column volumes of elution buffer (2× GIBCO 14200-075 PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Succinimidyl 6-(4, 4’-azipentanamido)hexanoate (LC-SDA; Thermo Scientific) crosslinking was performed by adding the reagent to final 1mM concentration ...