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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and A549 cells were maintained in DMEM (Invitrogen) supplemented with 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: Human Embryonic Kidney Grip TiteTM (HEK293-GT) cells (Invitrogen) was used to create a polyclonal cell line expressing GluA2(Q)i as previously described [49] ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were resuspended in buffer R (Thermo Fisher), along with the RNP complex and ssODN template ...
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bioRxiv - Biochemistry 2023Quote: The HEK293 cell line was obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were maintained in high-glucose DMEM (Gibco), supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTl- cells were cultured in Freestyle 293 (GIBCO) supplemented with 2% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... and HEK293 or RPMI 1640 (ATCC modification) (ThermoFisher, A1049101) (for Jurkat cells complete with 5% pen/strep and 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were grown in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... HEK293 cells were transfected using lipofectamine 2000 (Thermofisher, USA) following the manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were propagated in MEM with GlutaMAXTM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID: CVCL_6911) was purchased from Thermo Fisher Scientific (#R70007) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μl of the resulting 1:10 dilution were mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher) and subjected to library preparation for next-generation sequencing (vertis Biotechnologie) ...
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bioRxiv - Physiology 2019Quote: ... Strand-specific RNA-seq libraries containing unique index sequences in the adapter were generated simultaneously following the dUTP method using 700 ng total RNA and ERCC spike mix 2 (Ambion, 4456740). Libraries were pooled and sequenced on Illumina HiSeq 2500 in 75bp paired-end mode ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein (Hexapro antigen from Erica Ollmann Saphire, La Jallo for Immunology) was coupled to fluorescent streptavidin beads (Thermo Fisher) and incubated with sera samples to allow antibody binding to occur ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten- micro-liter of the aliquot was mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher). The resulting RNA samples were fragmented ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of RNA of each sample was mixed with 2 µl of ERCC ExFold spike-in RNA mixes based on the RNA ratio recommended by the protocol (Invitrogen 4456739), rRNA were depleted by hybridization of complementary DNA oligonucleotides and treatment with RNase and DNase ...
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bioRxiv - Bioengineering 2020Quote: ... The anti-polyhistidine antibody that was used in polyhistidine-mediated S1 protein immobilization (see Figure S1) was purchased from Thermo Fisher (MA1-21315). The horseradish peroxide (HRP ...
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bioRxiv - Biochemistry 2023Quote: ... Transformants were screened via colony PCR (Table S1, Fig. S1) using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific, USA) to confirm integration of donor DNA elements at the desired genomic locus and verified by sequencing ...
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bioRxiv - Immunology 2021Quote: ... The preparation of eVLPs expressing either Wuhan reference Spike or Beta variant Spike were formulated in Aluminum phosphate (Alum, Adjuphos®, Invitrogen) to obtain vaccine candidate VBI-2902a and VBI-2905a ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using D614G Spike and Delta Spike gene-specific primers and probes and TaqMan Fast Advanced Master mix (Cat# 4444963, Applied Biosystems) on a QuantStudio 5 system (Applied Biosystem) ...
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bioRxiv - Microbiology 2021Quote: ... and 0.144 µg pCG1-SARS-2-S or its derivative or 0.072 µg pCMV-VSVG as a control using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Immunology 2021Quote: RBDwt of SARS-CoV-2 and mutant RBDs (RBDK417N, RBDE484K, RBDN501Y, RBDK417N/E484K/N501Y, and RBDL452R/E484Q) were expressed using Expi293F cells (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: Phase diagrams of non-phosphorylated and phosphorylated NSARS-CoV-2 at different concentrations were determined using a NanoDrop spectrophotometer (ThermoFisher Scientific, Invitrogen). Increasing concentrations of polyU (0 - 2 µM ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated for 1 h with primary/detection SARS-CoV-2 anti-RBD rabbit polyclonal antibody (Sinobiologicals) and then 1 h with secondary anti-rabbit HRP-conjugate antibody (Invitrogen). Foci were visualised using TrueBlue™ Peroxidase Substrate (Sera Care ...
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bioRxiv - Pathology 2021Quote: ... 10 µl of rSARS-CoV-2 NL virus stock was diluted in 10 ml of OptiMEM media (ThermoFisher Scientific, #51985091) and then added to cells ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... 33 μM each of dGTP/dATP/biotin-11-dUTP/biotin-14-dCTP (Thermo Fisher), and 5 U Klenow in 1× NEB2 buffer at 37 °C for 45 min ...
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bioRxiv - Bioengineering 2021Quote: Biotin-PEG3-ONH2 (EZ-Link™ alkoxyamine-PEG4-biotin) was obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: 10 mM Biotin solution was prepared from EZ-Link Sulfo NHS-Biotin (Thermo Fisher). The solution was added to a 20-fold molar excess to 72 µM recombinant tau1-441 and incubated for 2 h on ice ...
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bioRxiv - Biophysics 2022Quote: ... 33 μM each of dGTP/dATP/biotin-11-dUTP/biotin-14-dCTP (Thermo Fisher), and 5 U Klenow in 1× NEB2 buffer at 37 °C for 45 min ...
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bioRxiv - Bioengineering 2021Quote: ... The quantification of reacted biotin was performed using the Pierce Biotin Quantification Kit (ThermoFisher). Biotinylated RBD was incubated with either streptavidin-conjugated PE (Biolegend ...
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bioRxiv - Molecular Biology 2020Quote: Antibodies were biotin labelled in-house using a biotin labelling kit (Thermo Fisher, #A39257) by following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The quantification of reacted biotin was performed using the Pierce Biotin Quantification Kit (ThermoFisher). Biotinylated RBD was incubated with either streptavidin-conjugated PE (Biolegend ...
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bioRxiv - Microbiology 2021Quote: Whole ear pinnae homogenates were diluted in 10-fold steps in LB medium and 10 µl of undiluted and diluted samples were pipetted onto sheep blood agar plates (Columbia Agar with sheep blood Plates; Oxoid, Cat. # PB0123A, Thermo Fisher Scientific, Waltham, Massachusetts). After incubation for 18 h at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... Chromatin complexes were precipitated with 100 µl of Dynabeads M-280 (Sheep anti-mouse, # 11202D; sheep anti-rabbit, #11204; Thermo Fisher Scientific, Waltham, MA). After washes and elution ...
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bioRxiv - Cell Biology 2020Quote: ... plus 10 % FCS (Gibco, Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1% FCS (Gibco). Followed by 3 days of culture ...
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bioRxiv - Bioengineering 2019Quote: ... FC from Life Technologies; and 2 ...
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bioRxiv - Neuroscience 2020Quote: ... containing 10% FCS (Invitrogen), and gentamycin (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... dialyzed FCS (Thermofisher, # 26400044), 28 μg/mL L-arginine-HCl (Arg0 or Arg10 ...
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bioRxiv - Biochemistry 2021Quote: ... including 10% FCS (Gibco), 2 mM glutamine (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... with 10 % FCS (Gibco), 2 mM glutamine (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... containing 10 % FCS (Gibco). Macrophages were seeded into 24-well plates at 105 cells per well.
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bioRxiv - Microbiology 2022Quote: ... containing 10% FCS (Gibco), 40 mM Hepes (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5% FCS (Gibco) in PBS with 0.01% Tween-20 (Sigma-Aldrich) ...