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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: A biotin tracer (EZ-Link™ NHS-Biotin; Thermo Fisher Scientific, Australia) was used to test the patency of the blood-testis barrier in Axdnd1-/- mice as described previously (44) ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotin tubulin was labeled with 2.8 mM Biotin-LC-LC-NHS (Invitrogen), and concentrations were determined using the molar extinction coefficient of 34,000 M-1 cm-1 [58] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Primers were designed to flank the intronic regions for the three Jheh genes (Table S1) and Phusion high fidelity DNA Polymerase (2 U/μL) (F-530XL Thermofisher Scientific) was used to amplify sequences ...
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bioRxiv - Developmental Biology 2022Quote: ... WIK 24hpf samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 1 (4456740, Thermofisher Scientific) while all other samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 2 (4456740) ...
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bioRxiv - Genomics 2019Quote: ... 1 μg of total RNA was mixed with 2 μl of 1:100 diluted ERCC RNA spike-in (Life Technologies), then ribosomal (r)RNAs were depleted using the RiboMinus Eukaryote v2 Kit (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... forward and reverse primers with overlapping SARS-CoV-2 spike sequence with the N501Y mutation followed by sequence complementary to the target plasmid (pEP-KanS) were synthesized (Invitrogen). A PCR fragment with overlapping ends carrying SARS-CoV-2 sequence flanking the Kanamycin resistance marker was amplified with the primers described using pEP-KanS as template ...
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bioRxiv - Cancer Biology 2019Quote: ... Input for each sample consisted of 500 ng of RNA and 5 μL of 1:500 diluted ERCC spike-in mix 2 (Ambion). Libraries were amplified for 11 cycles during the final amplification step ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was mixed with 2 µl of diluted ERCC RNA spike-in mix (1:100 dilution in nuclease-free H2O; Invitrogen). Ribosomal (r)RNAs were depleted using the Ribominus Eukaryote v2 kit (Ambion) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific). Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... PolyA+ RNA-seq libraries were prepared from 500 ng total RNA input supplemented with 2 μl 1:200 ERCC RNA Spike-In Mix (Invitrogen) using the TruSeq Stranded mRNA HT Kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The gel was then stained using Pro-Q Emerald 300 staining for glycoproteins kit (Invitrogen) following the procedure described by the supplier.
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... or HA-Tag (Invitrogen) antibody for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Biochemistry 2023Quote: ... anti-SNAP tag (Invitrogen), and DAPI ...
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bioRxiv - Systems Biology 2024Quote: ... TMT10plex tags (Thermo Scientific) were dissolved in anhydrous ACN and added to the peptide sample in a 1:10 peptide:TMT ratio ...
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bioRxiv - Cell Biology 2024Quote: ... anti-V5-tag (Invitrogen), HRP-conjugated anti-Rabbit (Abcam) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-V5-tag (Invitrogen), anti-GID8 (Proteintech) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 and OC43 were propagated in Vero E6 cells (ATCC) grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotin-azide (Molecular Probes) was conjugated to EdU by click chemistry for 2 h in click reaction buffer (10 mM sodium-L-ascorbate ...
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bioRxiv - Biochemistry 2022Quote: ... and biotin-cadaverine (ThermoFisher) were stored in DMSO at 10 mM at −20 °C ...
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bioRxiv - Biophysics 2022Quote: ... biotin-14-dCTP (Invitrogen) and biotin-14-dATP (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Vβ14 biotin (ThermoFisher/eBioscience) with streptavidin Qdot800 (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Avidin/Biotin block (Invitrogen), and protein block (Spring Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... AT8-biotin (ThermoFisher MN1020B) primary antibody was incubated with the sections at 1:500 dilution in the M.O.M ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotin-labeled-BSA (Invitrogen) at 100 µg mL-1 in BRB20 + 100 mM KCl was incubated in BD tissue culture-treated clear flat bottomed/black walled plates (FALCON ...
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bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar: BD Difco, Fisher Scientific; defibrinated sheep’s blood: Thermo Scientific, Fisher Scientific), and M9 minimal media (BD Difco ...
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bioRxiv - Immunology 2024Quote: ... We electroporated 3 μg of ODNs or gDNA into 2 × 106 HEK293 and 293A cells by Neon Transfection System (Thermo Fisher) based on the manufacturer’s introductions.
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Biochemistry 2022Quote: ... followed by C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For SARS-CoV-2 RBD VOCs proteins ...
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bioRxiv - Immunology 2022Quote: ... followed by a foldon trimerization motif and C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For competition ELISA experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... To 1/3 of the sample 1/10 of the recommended amount of spike-in (ERCC RNA spike-in mix, Ambion) was added ...
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bioRxiv - Immunology 2021Quote: ... Live CD3/CD8-CD14-CD19+IgM-IgD-IgG+Spike+Spike+ cells were sorted into individual wells containing RNase OUT (Invitrogen), First Strand SuperScript III buffer ...
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bioRxiv - Immunology 2023Quote: RNA was isolated using TRIfast [46] (Peqlab) with pre-isolation D.melanogaster Schneider S2 spike-in (1:10) and post-isolation ERCC spike-ins (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads Sheep anti-rat igG was obtained from Invitrogen, life technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-sheep Alexa 555-conjugated (Life Technologies, A21436) 1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... donkey anti-sheep 594 (1:500, Thermo Fisher, A11016). Slides were then washed 3 times for 10 min each in PBT and subsequently stained with 0.1 μg/ml DAPI in PBS for 10 min ...
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bioRxiv - Neuroscience 2019Quote: Alexa Fluro 488 donkey anti sheep (Life Technologies, A11015)
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bioRxiv - Microbiology 2019Quote: ... and Alexa Fluor 488-conjugated anti-sheep IgG (Invitrogen) are commercially available.
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bioRxiv - Plant Biology 2020Quote: ... Dynabeads M-280 Sheep Anti-Rabbit IgG (Invitrogen 11203D) or Anti-Mouse IgG (Invitrogen 11201D ...
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bioRxiv - Developmental Biology 2022Quote: ... and/or donkey anti-Sheep AlexaFluor-555 (ThermoFisher Scientific). In-situ antibody scheme modified from (Ronshaugen and Levine ...
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bioRxiv - Microbiology 2020Quote: ... and AlexaFluor647 targeting sheep-IgG (1:400, A21448, Invitrogen), and DAPI (1:1,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Alexa-647 Donkey anti-Sheep (ThermoFisher A21448, 1:350), Alexa-488 Donkey anti-Mouse (ThermoFisher A21202 1:750).
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bioRxiv - Neuroscience 2021Quote: ... IgG magnetic beads (Invitrogen; sheep anti-rabbit, cat.# 11202D) were incubated with an anti-Egr3 (rabbit polyclonal ...
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bioRxiv - Immunology 2022Quote: ... and then goat anti-sheep HRP (1:2000×, ThermoFisher) were added ...
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bioRxiv - Cell Biology 2019Quote: ... then incubated with anti-sheep Alexa-Fluor® (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Genomics 2020Quote: ... M-280 sheep anti-rabbit IgG Dynabeads (Invitrogen, 11203D) was used for chromatin immunoprecipitation ...
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bioRxiv - Biochemistry 2019Quote: ... added to sheep anti-Rat IgG-coupled Dynabeads (Invitrogen) pre-incubated with purified Rat Anti-Mouse CD31 (BD Pharmingen ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml defibrinated sheep blood (SR0051D, Thermo Fisher Scientific) was added and mixed (to sediment T cells in the next step) ...
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bioRxiv - Cell Biology 2020Quote: ... or Alexa Fluor 488 donkey anti-sheep (Life Technologies). Specimens were mounted using ProLong Gold antifade reagent (Life Technologies).