Labshake search
Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... was transduced into HEK293 cells using Lipofectamine LTX (Invitrogen-ThermoFisher) to knock out PML by the CRISPR-Cas9 system ...
-
bioRxiv - Cell Biology 2023Quote: ... was transduced into HEK293 cells using Lipofectamine LTX (Invitrogen-ThermoFisher) to knock out PML by the CRISPR-Cas9 system ...
-
bioRxiv - Neuroscience 2023Quote: HEK293 and GL261 cells were cultured in DMEM (Gibco, #31885) with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2023Quote: ... GKO HEK293 cells were maintained in DMEM (Gibco Cat# 11995065) containing 10% FBS (Gibco Cat# 16000044) ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293 Flp-In T-Rex cells (Thermo Fisher Scientific, R78007) were transfected with pcDNA5/FRT/TO-GFP (see below ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293 Flp-In T-REx cells (Thermo Fisher Scientific, R78007) with SBP-tagged eIF4A1 integrant 15 were seeded in a 10-cm dish and cultured for 3 d in the presence of 1 μg/ml tetracycline ...
-
bioRxiv - Cancer Biology 2023Quote: ... HEK293 in Dulbecco’s modified Eagle Medium (DMEM, Life Technologies #11965118); T-47D in Roswell Park Memorial Institute (RPMI ...
-
bioRxiv - Cell Biology 2023Quote: Flp-In™ T-REx™ HEK293 cells (Invitrogen, R78007) containing a single genomic FRT site and stably expressing the Tet repressor were cultured in DMEM medium supplemented with 10% FBS ...
-
bioRxiv - Systems Biology 2023Quote: ... HEK293 cell lines were lifted using TrypLE Express (Thermo Fisher) and resuspended in media supplemented with the appropriate volume of cADDis BacMam ...
-
bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected with the NeonTM transfection kit (Invitrogen) according to the manufacturer’s description ...
-
bioRxiv - Cell Biology 2024Quote: Flp-In T-Rex HEK293 cells (Invitrogen, Thermo Fisher Scientific) were co-transfected with plasmids pOG44 (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: Flp-In T-Rex HEK293 cells (Invitrogen, Thermo Fisher Scientific) were co-transfected with plasmids pOG44 (Invitrogen ...
-
bioRxiv - Biophysics 2023Quote: ... HEK293 GnTI- cells were cultured in Freestyle 293 medium (Invitrogen) supplemented with 2% FBS on a shaker at 37 °C in the presence of 8% CO2 to a density of 3 × 106 cells per ml ...
-
bioRxiv - Biochemistry 2024Quote: ... GKO HEK293 cells were maintained in DMEM (Gibco Cat# 11995065) containing 10 % FBS (Gibco Cat# 16000044) ...
-
bioRxiv - Cell Biology 2024Quote: FlpIn CHO and HEK293 cell lines were purchased from Invitrogen and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 5% Foetal Bovine Serum (ThermoFisher Scientific (Gibco) ...
-
bioRxiv - Developmental Biology 2024Quote: HEK293 cells were transfected using Lipofectamine 3000 (Invitrogen, Cat# L3000008) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Flp-In T-Rex HEK293 cell lines (Thermo Fisher Scientific) expressing mitochondrial-relocated golgin-BirA* fusions (from John Shin ...
-
bioRxiv - Neuroscience 2023Quote: ... Stable HEK293 lines were generated by lipofection (Lipofectamine 2000, ThermoFisher) of ∼80% confluent cells with 2 µg of linearized expression plasmid overnight according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... and glucosylated species were detected using the periodic acid-Schiff (PAS) method (Glycoprotein staining kit, Thermo Scientific).
-
bioRxiv - Neuroscience 2023Quote: The isolated glycoproteins were dried in a vacuum centrifuge overnight (Savant™ SPD131DDA SpeedVac™ Concentrator, ThermoFisher). These were immobilized in an acrylamide gel and reduced and alkylated ...
-
bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
-
bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
-
bioRxiv - Cell Biology 2020Quote: ... secondary antibody (AlexaFluor488 donkey anti mouse IgM 1/400, Life Technologies in Buffer A, 0.1% Saponin 2% FCS) was performed ...
-
bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
-
bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2.
-
bioRxiv - Immunology 2020Quote: ... and lymphocytes were resuspended in complete RPMI (cRPMI- RPMI + 10% FCS + 2% Penicillin-Streptomycin + 1% L-glutamine (Gibco)) ...
-
bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies ...
-
bioRxiv - Developmental Biology 2023Quote: ... Bones were flushed using a 26-gauge needle of a 1-ml syringe with HBSS+2% FCS (Gibco). BM clumps were re-suspended by gentling aspirating with a 19-gauge needle ...
-
bioRxiv - Immunology 2022Quote: ... These cells were cocultured with A549-ACE2-A2402 cells expressing each spike protein an E:T ratio of 2:1 and incubated with RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS at 37°C for 6 h ...
-
bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
-
bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was biotinylated using N-hydroxysuccinimide polyethylene glycol biotin (NHS-PEG4-biotin, ThermoFisher) by mixing 5.6 μM claudin-4 with 16.8 μM NHS-PEG4-biotin ...
-
bioRxiv - Bioengineering 2021Quote: ... Unoccupied biotin binding sites were then blocked by addition of 100 mM D-biotin (Thermo Fisher) in dimethyl sulfoxide (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... The probes were labelled with biotin using Biotin 3’ End DNA Labeling Kit (Thermo scientific, USA). Hybridization signals were detected by Chemiluminescent Nucleic Acid Detection Module (Thermo scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... Biotin 3’ end-labeled DNA oligomers were prepared using a biotin end-labeling kit (Thermo Scientific), and double-stranded DNA probes were generated by annealing sense and antisense oligomers ...
-
bioRxiv - Immunology 2019Quote: ... the peptides were labelled with biotin by using Sulfo-NHS-LC-Biotin (Thermo Scientific, IL, USA). The unreacted Sulfo-NHS-LC-Biotin was quenched by 1 M glycine solution ...
-
bioRxiv - Molecular Biology 2023Quote: ... were used following endogenous biotin blocking using Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
-
bioRxiv - Molecular Biology 2023Quote: ... were used following endogenous biotin blocking using Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
-
bioRxiv - Neuroscience 2022Quote: ... Donkey-anti-Sheep 647 (1:1000, Thermo Fisher Scientific, A-21448), Goat anti-Chicken IgY (H+L ...
-
bioRxiv - Neuroscience 2019Quote: ... with the magnetic sheep anti-rabbit beads (Invitrogen Corp., Carlsbad, CA) at 4°C overnight on a rotator ...
-
bioRxiv - Cell Biology 2021Quote: ... was incubated with sheep anti-rat IgG magnetic dynabeads (Invitrogen, 11035) in a solution of sterile PBS 0.1%BSA (120ul of beads ...
-
bioRxiv - Neuroscience 2020Quote: ... and Alexa Fluor 568 donkey anti-sheep IgGs (1:200, Invitrogen). Sections were counterstained using bis-benzamide (1:10,000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by incubation with Alexa Fluor 555 donkey anti-sheep (Invitrogen) and Alexa Flour 488 goat anti-mouse (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... donkey-anti-sheep AF 488 (Thermo Fisher # A-11015, 1:500); donkey-anti-mouse AF 488 (Thermo Fisher #A-21202 ...
-
bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 555 donkey-anti-sheep IgG (1:500; A21436, Invitrogen).
-
bioRxiv - Biochemistry 2022Quote: 300 μl M280 Dyna beads sheep anti-rabbit IgG (Life technologies) was added to 1 ml freshly prepared PBS with 5 mg/ml BSA (PBS/BSA ...
-
bioRxiv - Developmental Biology 2019Quote: ... amplified with anti-sheep Alexa Fluor 488 (1:100, Molecular Probes) and biotinylated anti-Avidin (1:100 ...
-
bioRxiv - Immunology 2020Quote: ... 0.5 ml defibrinated sheep blood (Thermo Scientific Oxoid, catalog no. SR0051D) was added to deplete T cells in the next step ...
-
bioRxiv - Immunology 2020Quote: ... After blocking with 100 μl of sheep serum (16070-096, Gibco), the sera from COVID-19 patients were serially diluted from 10- to 31,250-fold in blocking buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... and sheep IgG (1:500 for IF) were from Thermo Fisher Scientific ...