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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were cultured in DMEM (Life Technologies), supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC) were grown in DMEM (Gibco) with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FCS (FCS; Invitrogen; Thermo Fisher Scientific, Inc.). When the cultured cells reached 70-80% confluence at 37° C in a humidified 5 %CO2 incubator ...
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bioRxiv - Genomics 2023Quote: ... the plate containing the lysed cells is supplemented with RNA spike-ins (1 μL of a 1:1,600,000 dilution of ERCC spike-in mixture A (Life Technologies)) ...
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bioRxiv - Bioengineering 2020Quote: ... After incubation with excess streptavidin and biotin to block endogenous biotin (Endogenous Biotin Blocking Kit; Thermo Fisher Scientific, Waltham, MA), 5 µM of biotinylated HCDR3-construct was incubated on tissue overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: ... These cells were cultured for 24 hours with either anti-CD3 biotin (Miltenyi)/anti-CD28 biotin (Miltenyi) or anti-CD3 biotin (Miltenyi)/aCD6 biotin (LSBio) on streptavidin coated 96 well plates (ThermoFisher) to yield 50% CD69 expression (antibody amount required determined by prior experimentation) ...
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bioRxiv - Genomics 2023Quote: ... PBMCs were cultured for 24 hours with either anti-CD3 biotin (Miltenyi)/anti-CD28 biotin (Miltenyi) or anti-CD3 biotin (Miltenyi)/aCD6 biotin (LSBio) on streptavidin coated 96 well plates (ThermoFisher) to yield 50% CD69 expression on CD4+ T cells (antibody amount required determined by prior experimentation) ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Microbiology 2022Quote: ... biotin incorporation was measured using the Pierce Biotin quantitation kit (ThermoFisher Scientific). For measuring the incorporation of the SULFO-TAG ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotin tubulin was labeled with 2.8 mM Biotin-LC-LC-NHS (Invitrogen), and concentrations were determined using the molar extinction coefficient of 34,000 M-1 cm-1 [58] ...
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bioRxiv - Cell Biology 2023Quote: A biotin tracer (EZ-Link™ NHS-Biotin; Thermo Fisher Scientific, Australia) was used to test the patency of the blood-testis barrier in Axdnd1-/- mice as described previously (44) ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was diluted with 400 μl of NET-2 buffer and antibody-conjugated Dynabeads M-280 Sheep anti-mouse IgG (ThermoFisher Scientific) beads were added ...
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bioRxiv - Microbiology 2021Quote: ... 4 % FCS (Gibco), 200 units/mL penicillin and 0.2 mg/ mL streptomycin ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS (InVitrogen) and either 1 µg/mL puromycin (DIvA cells ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Gibco), 1% non-essential amino acid (NEAA ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (Gibco) in RPMI ...
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bioRxiv - Cancer Biology 2022Quote: ... 5% FCS (Invitrogen), 5% human serum (HS) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FCS (Gibco), 1X Pen-Strep (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 % FCS (Gibco), 1 % PenStrep (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... 44% FCS (Gibco), and 6% DMSO (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... 5% FCS (Gibco), and 1x penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2024Quote: ... 10% FCS (Gibco), penicillin/streptomycin ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Primers were designed to flank the intronic regions for the three Jheh genes (Table S1) and Phusion high fidelity DNA Polymerase (2 U/μL) (F-530XL Thermofisher Scientific) was used to amplify sequences ...
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bioRxiv - Developmental Biology 2022Quote: ... WIK 24hpf samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 1 (4456740, Thermofisher Scientific) while all other samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 2 (4456740) ...
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bioRxiv - Genomics 2019Quote: ... 1 μg of total RNA was mixed with 2 μl of 1:100 diluted ERCC RNA spike-in (Life Technologies), then ribosomal (r)RNAs were depleted using the RiboMinus Eukaryote v2 Kit (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... forward and reverse primers with overlapping SARS-CoV-2 spike sequence with the N501Y mutation followed by sequence complementary to the target plasmid (pEP-KanS) were synthesized (Invitrogen). A PCR fragment with overlapping ends carrying SARS-CoV-2 sequence flanking the Kanamycin resistance marker was amplified with the primers described using pEP-KanS as template ...
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bioRxiv - Cancer Biology 2019Quote: ... Input for each sample consisted of 500 ng of RNA and 5 μL of 1:500 diluted ERCC spike-in mix 2 (Ambion). Libraries were amplified for 11 cycles during the final amplification step ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was mixed with 2 µl of diluted ERCC RNA spike-in mix (1:100 dilution in nuclease-free H2O; Invitrogen). Ribosomal (r)RNAs were depleted using the Ribominus Eukaryote v2 kit (Ambion) ...
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bioRxiv - Genetics 2020Quote: ... or HA-Tag (Invitrogen) antibody for 1 h at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... TMT10plex tags (Thermo Scientific) were dissolved in anhydrous ACN and added to the peptide sample in a 1:10 peptide:TMT ratio ...
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bioRxiv - Biochemistry 2023Quote: ... anti-SNAP tag (Invitrogen), and DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotin-azide (Molecular Probes) was conjugated to EdU by click chemistry for 2 h in click reaction buffer (10 mM sodium-L-ascorbate ...
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bioRxiv - Biochemistry 2022Quote: ... and biotin-cadaverine (ThermoFisher) were stored in DMSO at 10 mM at −20 °C ...
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bioRxiv - Biophysics 2022Quote: ... biotin-14-dCTP (Invitrogen) and biotin-14-dATP (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Vβ14 biotin (ThermoFisher/eBioscience) with streptavidin Qdot800 (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... AT8-biotin (ThermoFisher MN1020B) primary antibody was incubated with the sections at 1:500 dilution in the M.O.M ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotin-labeled-BSA (Invitrogen) at 100 µg mL-1 in BRB20 + 100 mM KCl was incubated in BD tissue culture-treated clear flat bottomed/black walled plates (FALCON ...
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bioRxiv - Cell Biology 2023Quote: ... Avidin/Biotin block (Invitrogen), and protein block (Spring Bioscience) ...
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bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar: BD Difco, Fisher Scientific; defibrinated sheep’s blood: Thermo Scientific, Fisher Scientific), and M9 minimal media (BD Difco ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Immunology 2024Quote: ... We electroporated 3 μg of ODNs or gDNA into 2 × 106 HEK293 and 293A cells by Neon Transfection System (Thermo Fisher) based on the manufacturer’s introductions.
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 and OC43 were propagated in Vero E6 cells (ATCC) grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... followed by C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For SARS-CoV-2 RBD VOCs proteins ...
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bioRxiv - Immunology 2022Quote: ... followed by a foldon trimerization motif and C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For competition ELISA experiments ...
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bioRxiv - Microbiology 2020Quote: ... The gel was then stained using Pro-Q Emerald 300 staining for glycoproteins kit (Invitrogen) following the procedure described by the supplier.
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... biotin-conjugated secondary antibodies (Biotin-XX Goat anti-Mouse IgG B2763, or Biotin-XX Goat anti-Rabbit IgG B2770, Thermo Fisher) were utilized at a dilution of 1:750 ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads Sheep anti-rat igG was obtained from Invitrogen, life technologies ...