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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... similarly as described previously.[60] MS data were analyzed using an in-house maintained human protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific). The mass tolerances of a fragment ion and a parent ion were set as 0.5 Da and 10 ppm ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2023Quote: Plasmids coding for the soluble S ectodomain proteins as well as the RBD of human coronaviruses were transfected into Freestyle293F cells (Thermo Fisher R79007). Per 1 L of Freestyle 293F cells ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
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bioRxiv - Biochemistry 2020Quote: ... The extracted FFAs were then determined using a Thermo Scientific TRACE 1300 gas chromatograph (GC) equipped with a TG-WaxMS A column (30 m × 0.32 mm × 0.25 µm; Thermo Scientific) and a Flame Ionization Detector (FID ...
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bioRxiv - Microbiology 2019Quote: ... vectors cloned with gene encoding Gn or Gc were transfected into HEK293F cells (Thermo Fisher Scientific, Waltham, MA, USA) using polyethylenimine ...
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bioRxiv - Molecular Biology 2022Quote: ... inlet into a Thermo Trace gas chromatograph fitted with a Trace TR-50MS GC column (60 m × 0.25 mm, 0.25 μm film thickness, Thermo Fisher). Analytes were separated with helium as the carrier gas at a constant flow of 1.2 ml/min with vacuum compensation ...
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bioRxiv - Molecular Biology 2021Quote: Lipid analysis was performed with a TRACE 1310 GC–ISQ single quadrupole MS (Thermo Fisher Scientific, Waltham, MA, USA). In brief ...
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bioRxiv - Plant Biology 2021Quote: ... The analysis of yeast and plant extracts was carried out using a Trace GC Ultra gas chromatograph (Thermo Scientific) coupled to an ATAS Optic 3 injector and an ISQ single quadrupole mass spectrometer (Thermo Scientific ...
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bioRxiv - Plant Biology 2022Quote: Samples were analyzed on the Thermo Scientific™ Q-Exactive™ GC Orbitrap™ (Fisher scientific, Waltham, MA, USA). Samples were injected into a Thermo Scientific TraceGOLD TG-5SILMS column that was 30 m long ...
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bioRxiv - Microbiology 2023Quote: ... 30m x 0.25mm ID x 0.25μm df supplied by GC-MS Trace 1310-ac /MS TSQ-900 (Thermo Scientific, USA). The oven program was as follows ...
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bioRxiv - Immunology 2023Quote: ... Genomic copy number (GC) of AAV was determined by real-time qPCR using custom TaqMan assays (Thermo Fisher Scientific) targeted to EFS promoter.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Gas chromatographic separation was conducted on a ThermoFisher Scientific Trace 1300 GC with a TraceGold TG-5SilMS column (0.25 μm film thickness; 0.25 mm ID; 30 m length; ThermoFisher Scientific), as described (Tompkins et al. ...
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bioRxiv - Genomics 2023Quote: Total RNA was isolated from individual GC wells using the TRIzol TM reagent (Thermo Fisher Scientific, Waltham, MA USA)) and the Direct-zol RNA kit (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... The mandibular gland pheromone mixes were separated by a GC-MS system (Agilent 6890N/5973I, Thermo Fisher Scientific, USA), which was equipped with an HP-INNOWax capillary column ...
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bioRxiv - Cell Biology 2024Quote: ... The region surrounding the CRISPR/Cas9 target site was PCR amplified using AccuPrime GC-Rich DNA Polymerase (Thermofisher 12337024) (Forward Primer ...
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bioRxiv - Plant Biology 2024Quote: ... split flow 6.0 mL/min) onto a TG-5SILMS GC Column (30 m × 0.25 mm × 0.25 µm; Thermo Fisher Scientific), using helium as a carrier gas at a constant flow of 1.2 mL/min ...
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bioRxiv - Microbiology 2024Quote: ... and the GC-MS analysis was carried out in 30 - 550 mass range scanning mode (Thermo Scientific ISQ 7000).
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Cell Biology 2020Quote: ... Ki-67 recombinant rabbit monoclonal antibody (SP6) (Thermofisher, Cat. #: MA5-14520), Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant soluble ACE2 was coated on NUNC Maxisorp plates (Thermo Scientific) at 1µg/well at RT for 3 h ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant gene was cloned into pFastBac1 expression vector (Life Technologies) for bacmid production ...
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bioRxiv - Cell Biology 2019Quote: Recombinant Bax was labeled by NBD dye (IANBD amide, Life Technologies) as reported previously (Kale et al. ...
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bioRxiv - Genetics 2021Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
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bioRxiv - Biochemistry 2021Quote: ... the isolated recombinant EMBacY was transfected into adhesive Sf9 cells (Invitrogen) in 6-well plates ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 U of recombinant Taq DNA Polymerase (Thermo Fisher Scientific, Lithuania) and water to final volume of 10μl ...
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bioRxiv - Cancer Biology 2021Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher, Cat. No. 10777019) and incubated on ice for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... We used GFP recombinant rabbit monoclonal antibody (G10362, Thermo Fisher Scientific) at 1:300 dilution and monoclonal anti-GFP ...
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bioRxiv - Immunology 2021Quote: ... and 1µl of RNaseOUTTM Recombinant Ribonuclease Inhibitor (Invitrogen, Catalogue no.-10777019). 11µl of the cocktail was added to the RNA/primer mix ...
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bioRxiv - Cancer Biology 2021Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher, Cat. No. 10777019) and the lysates were incubated on ice for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... then treated with recombinant DNaseI (DNA-free DNA removal kit, Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant soluble DPP4 was coated on NUNC Maxisorp plates (Thermo Scientific) at 100 ng/well at RT for 3 h ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed using Taq DNA Polymerase Recombinant kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Recombinant mAbs were then produced in EXPi293F cells (Life Technologies, USA) by transfecting pairs of the IgG1 heavy and light chain expression plasmids ...
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bioRxiv - Microbiology 2022Quote: ... The full Delta-Omicron recombinant spike was cloned into pcDNA6 (Invitrogen). Point mutations were introduced by overlap extension PCR ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng/ml mouse recombinant LIF (ES cell-tested) (Gibco #A35935). Upon reaching confluence ...
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bioRxiv - Molecular Biology 2023Quote: All PCRs were performed by using Recombinant Taq polymerase (Thermo Scientific). 100 ng of the synthesized DNA was mixed with 1X PCR buffer ...
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bioRxiv - Neuroscience 2022Quote: ... or 20mg/mL proteinase K (recombinant PCR grade, ThermoFisher Cat# EO0492) for 10 ...
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bioRxiv - Neuroscience 2022Quote: ... and recombinant rabbit monoclonal anti-LUM (Lumican, Invitrogen Cat#MA5-29402). The samples were subsequently digitized using a NanoZoomer Hamamatsu S60 digital slide scanner.
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bioRxiv - Synthetic Biology 2024Quote: ... Staining with DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, 701629) at 1:500 dilution was performed in blocking solution at 4℃ for 14 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin/streptomycin and 50ng/ml recombinant mouse EGF (Life Technologies) was used for culturing colon organoids ...
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bioRxiv - Immunology 2024Quote: ... injections also contained 1 μg of recombinant murine IL-2 (Gibco). PBS was used as vehicle.
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bioRxiv - Biochemistry 2024Quote: ... Recombinant murine pro-CtsK were expressed in 293-Freestyle cells (ThermoFisher) by transient expression using FectoPRO transfection reagent (Polyplus transfection) ...
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bioRxiv - Biophysics 2024Quote: ... The recombinant DR2539 was expressed in Escherichia coli BL21 (DE3) (Invitrogen). The cells were grown at 310 K to an OD600 of ≈ 0.6 in Luria-Bertani medium containing 100 µg ml-1 of ampicillin ...
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bioRxiv - Immunology 2023Quote: Recombinant gp120s were produced via transient transfection using Freestyle 293F (ThermoFisher) or 293S GnT1- cells and 293Fectin using the same conditions as SOSIP gp140s ...