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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Recombinant Human GC Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant viruses were generated using the Bac-to-Bac Baculovirus expression system (Invitrogen). The proteins were expressed by infecting 50 ml of Sf9 insect cells at density of 1.0 x 106 ml−1 with the above-described viruses ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant baculoviruses were generated using the Bac-to-Bac baculovirus expression system (Invitrogen) and the proteins were expressed in Sf9 insect cells infected at a density of 1.2 x 106 ml−1 with P3 baculovirus stocks in a 1:1 (v/v ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was removed with recombinant DNase (TURBO DNA-free™ Kit, Ambion). Concentration and purity of RNA were both evaluated with NanoDrop spectrophotometer ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cells for recombinant antigen and monoclonal antibody production (Thermo Fisher Scientific, A1452) were maintained at 37°C in 8% CO2 in Expi293F expression medium (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... and ligated to produce recombinant constructs using T4 DNA ligase (Thermo Fisher Scientific). Ligation reactions contained 50 ng of vector ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection ...
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bioRxiv - Neuroscience 2022Quote: ... recombinant bacmid DNA was purified and transfected into adherent Sf9 cells (Thermo Fisher) using Cellfectin reagent to generate P1 recombinant baculovirus stock ...
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bioRxiv - Neuroscience 2024Quote: For recombinant lentivirus production - HEK293FT cell line (cat# R70007, ThermoFisher Scientific, Auckland, NZ), HT1080 cell line (cat# CCL-121 ...
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bioRxiv - Genomics 2022Quote: ... Recombinant plasmids were transfected into cell lines using Lipofectamine 2000 (Invitrogen; cat.no: 11668019). Vector backbone and unmethylated constructs were used as internal controls for comparison ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant T-REx-HEK-CFTR cells were generated by lipofectamine transfection (Life Technologies) of plasmids containing mutated versions of CFTR (RefSeq NM_000492) ...
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bioRxiv - Biochemistry 2023Quote: ... 6 µg RNA was used and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant chimeric antibodies were produced using the Expi293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Physiology 2023Quote: ... Recombinant TEVp was purified as previously described1 or was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50 ng of recombinant Gag/well (35) in 1xPBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and incubated with recombinant enzymes TrypLE (Thermo Fisher) for 10 minutes at 37°C for single cell dissociation ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 ng/ml recombinant noggin (Thermo Fisher Scientific, cat# 120-10C-250UG). The 3dGRO™ human ISO72 colorectal cancer tumoroid line was purchased from Millipore Sigma (Burlington ...
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bioRxiv - Cell Biology 2022Quote: Affymetrix Human ClariomD data were normalized using quantile normalization with adjustment based on the median intensity of probes with similar GC content (using Affymetrix Power Tools). Background correction was made using the antigenomic probes ...
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bioRxiv - Genomics 2020Quote: ... and LECs were treated for additional 24h with 20nM of high GC scramble control siRNA or two siRNAs targeting KLF4 (Thermo Fisher Scientific) and 32µL Lipofectamine RNAiMAX previously mixed in 800µL Opti-MEM.
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bioRxiv - Microbiology 2019Quote: ... interfaced with a Scientific GC IsoLink II Conversion Unit connected to an IRMS DELTA V Advantage Isotope-ratio mass spectrometer (Thermo Fisher Scientific). The gas chromatograph was equipped with a fused silica capillary column (25 m x 0.32 mm ...
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bioRxiv - Plant Biology 2021Quote: FA methyl esters analysis was performed using a TRACE GC ULTRA gas chromatograph system coupled with a DSQ II quadrupole mass spectrometer (Thermo Scientific, France) and equipped with an automated sample injector ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were derivatized and analyzed on an Agilent GC-MS as described above or directly analyzed on a QExactive Plus Orbitrap (Thermo Fisher Scientific) equipped with a HESI probe and a Vanquish Horizon UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The extracts were sent to the Department of Chemistry Mass Spectrometry core facility at Texas A&M University for gas chromatography-mass spectrometry (GC-MS) on a DSQ II (Thermo Scientific, TX), and liquid chromatography-mass spectrometry (LC-MS ...
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bioRxiv - Microbiology 2021Quote: ... Chromatographic separation was performed by injecting 1 µl of farnesol standard onto a Thermo TSQ™ 9000 Triple Quadrupole GC-MS/MS System (Thermo Scientific) set to splitless ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... QBIHEK293 cells that expressed GC-A and GC-B were split the day before the experiment and harvested using an ethylenediaminetetraacetic acid (EDTA) solution (Versene, Invitrogen, ThermoFischer Scientific). Cells (6000/well GC-A-expressing ...
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bioRxiv - Genetics 2021Quote: ... The granulosa cells (GCs) were isolated from the hierarchical follicles and then dispersed by treatment with 0.25% trypsin-EDTA (Gibco, Camarillo, CA, USA) at 37 °C for 10 min with gentle oscillati on in a centrifuge tube ...
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bioRxiv - Genomics 2021Quote: ... and lines were genotyped using primers listed in Table S1 and Phusion High-Fidelity PCR Master Mix (with GC Buffer) (Thermo Scientific, F532). PCR products of candidate clonal lines showing predicted PCR band sizes in agarose gel electrophoresis ...
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bioRxiv - Microbiology 2022Quote: ... to molten Gonococci agar (GC agar) before pouring rectangular assay plates with the same dimensions as the standard 96-well microplates (Thermo Scientific Nunc # 267060). The GC agar recommended for N ...
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bioRxiv - Developmental Biology 2023Quote: ... we slowly injected 0.5-1 μL rAAV (about 1∼3×109 genome copy (GC)) mixed with Chicago Sky Blue dye (0.1%, Fisher Scientific, Cat # AAA1424214) into the oviduct ampulla using a glass micropipette with tip diameter of ∼10-30 μm ...
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bioRxiv - Plant Biology 2023Quote: ... performed using a selective mass detector with ion trap (Trace GC 2000 series coupled to a Thermo Scientific Polaris Q mass spectrometer), enabled the identification of the derivatized suberin monomers comparing the mass spectra with available standards or literature (Kolattukudy and Agrawal ...
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bioRxiv - Plant Biology 2024Quote: ... 1µl of extracts were injected directly into the GC–MS for analysis on a Trace GC Ultra gas chromatograph coupled to an ATAS Optic 3 injector and an ISQ single quadrupole mass spectrometer (Thermo Fisher Scientific) with electron impact ionization ...
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bioRxiv - Biochemistry 2023Quote: ... The cDNA was mixed into a 50 μL second strand synthesis reaction containing 10 μL 5X GC Phusion Buffer (Thermo Fisher Scientific), 1 μL 10 mM dNTP ...
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bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
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bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... Total IgG in human sera was measured using a commercial human IgG ELISA kit (Fisher Scientific) according to manufacturer’s protocol.