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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The cleared lysate was incubated for 2 h at 4 °C with Ni-NTA agarose resin (Invitrogen) under slight agitation ...
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bioRxiv - Cell Biology 2021Quote: ... then digested while shaking at 37°C for 2 hours in 200 U/mL Collagenase I (Gibco) + 1 mg/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated for 2 hours at 4° C in either DyLight 800 goat anti-rabbit IgG (Invitrogen) or DyLight 800 rabbit anti-mouse IgG (Rockland Immunochemicals ...
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bioRxiv - Plant Biology 2019Quote: ... Germany) followed by a 2 hour digestion at 37°C with the enzyme HpyF3I (DdeI) (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... stratified for 2 days at 4°C and grown for 11 days in a Sanyo (Fisher Scientific) under short-day conditions (8 h light ...
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bioRxiv - Genomics 2021Quote: ... for 2 hours at 37°C followed by 10 uL 20 mg/mL proteinase K (Invitrogen 25530049) for 45 minutes at 55°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for 20 minutes at 37°C and transferred to custom-made 2% agarose (UltraPure; Thermo Fisher Scientific) casting molds in 24-well plate formats ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then incubated with rotation for 2 hours (at 4°C) with Talon cobalt resin (Thermofisher). After incubation ...
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bioRxiv - Neuroscience 2022Quote: ... 2 vials for each genotype were rapidly thawed and added to pre-warmed (37°C) medium (Gibco Minimal Essential Medium with 10% fetal calf serum and 1X penicillin streptomycin) ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 hours at 37°C followed by Alexa fluor rat 594 secondary antibody (A-11007, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Lysates were then incubated with rotation for 2 hours (at 4°C) with Talon cobalt resin (Thermofisher). After incubation ...
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bioRxiv - Cell Biology 2023Quote: ... at 150 × 106 cells/dish for 2 h at 37°C/5% CO2 in RPMI-1640 (Gibco) to separate adherent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... and BE(2)-C were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
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bioRxiv - Biophysics 2023Quote: ... A preheated (90°C, 1 min) number 1 German borosilicate sterile 2 well chamber coverglass slide (ThermoFisher) was loaded with 750 μL of melted 0.7% Omnipur agarose (EMD Millipore) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cοtransfected with 7 μg CRISPR plasmid and 7 μg non-linearized HDR template using Lipofectamine 3000 according to the instructions (Thermo Fisher). 48 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... and the cell pellet was resuspended in annexin buffer with Annexin V-FITC and 7-AAD according to the manufacturer’s specifications (FITC Annexin V 7-AAD-Thermo Fisher®). Flow cytometry was performed using Attune flow cytometer (Thermo Fisher®) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with FACS buffer and incubated with the live-dead marker 7-aminoactinomycin D (7-AAD; Thermo Fisher Scientific) for 10 min at 4 °C in the dark ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 hMSCs from 4 donors chosen randomly (2 male; B, G, and 2 female; C, D) were thawed and cultured in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) supplemented with RoosterNourishTM-MSC expansion medium (RoosterBio ...
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bioRxiv - Cell Biology 2020Quote: ... HPLC analysis was performed employing a C30 reverse-phase column (Thermo Acclaim C30, 2.1 x 250 mm, 3 µm, operated at 40° C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...
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bioRxiv - Biophysics 2021Quote: ... Grids were blotted for 3 s with a blotting force of 3 and 100% humidity at 22 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher).
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bioRxiv - Neuroscience 2021Quote: ... 20 μg/ml in sterile water Gibco 10977035, 1 hour at 37°C followed by 3 washes with PBS Gibco 10010015) / Laminin (Gibco 23017-015 ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated cells were collected by centrifugation (200 g, 3 min at 25 °C) and resuspended in complete medium comprising neurobasal medium (Gibco, 21103) supplemented with 2% B-27 plus (Gibco A3582801) ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at RT and then incubated for 3 days at 4°C with the monoclonal mouse anti-NeuN antibody conjugated with Alexa-488 (clone A60, Life Technologies) at a concentration of 0.5 µg mL-1 into blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... the RNA samples were fragmented with FastAP buffer at 94 °C for 3 min and were dephoshorylated using the FastAP enzyme (Thermo Scientific) at 37 °C for 30 min followed by bead purification with 2 volumes of Agencourt RNAClean XP beads ...
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bioRxiv - Biophysics 2021Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Microbiology 2020Quote: ... of 0.001-0.01 and amplified for 1-3 hours at 37 °C at 250 rotations per minute (rpm) (MaxQ4000 shaker, Thermo Fisher Scientific) until lysis was observed ...
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bioRxiv - Immunology 2022Quote: ... Proteins and DNAs were incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 cycle at 72°C for 3 min using a Veriti Thermal Cycler (Applied Biosystems, Foster City, CA, USA). The PCR products were analyzed by heteroduplex mobility assay using microfluidic capillary electrophoresis system caliper LabChip GX (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... 3 μm C 18 beads (Nikkyo Technologies, NTCC-360/75-3-123°Column) with buffer A: 0.1% formic acid (Fisher Scientific, A11750), and buffer B ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... HPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 × 250 mm, 3 μm, operated at 50°C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC system(Agilent ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Genetics 2020Quote: ... HPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 × 250 mm, 3 μm, operated at 50°C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... after denaturation by 70°C for 3 minutes and separated by gel electrophoresis using 10% TBE-Urea gels (Invitrogen, Cat# EC68755BOX) at 180V for 60 min in 1x TBE electrophoresis buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... XmaI+ClaI.SV40-pA 5’-ATG CAT GCA TCC CGG GCA TCG ATG CAG TGA AAA AAA TGC TTT ATT TGT GAA ATT TGT GAT GCT ATT GCT TTA TTT GTA ACC-3’ and SV40-pA.EcoRI+XbaI 5’-ATG CAT GCA TGA ATT CGT CTA GAA ACT TGT TTA TTG CAG CTT ATA ATG GTT ACA AAT AAA GCA ATA GCA TCA C-3’ and PCR SuperMix Taq (Invitrogen #10572014), we amplified a 128 bp fragment (5’-ATG CAT GCA TCC CGG GCA TCG ATG CAG TGA AAA AAA TGC TTT ATT TGT GAA ATT TGT GAT GCT ATT GCT TTA TTT GTA ACC ATT ATA AGC TGC AAT AAA CAA GTT TCT AGA CGA ATT CAT GCA TGC AT-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Immunology 2021Quote: ... 200,000 cells from each B cell subset were sorted into pure FBS on ice and immediately washed 3 times in 37°C PBS and labeled with CellTrace carboxyfluorescein succinimidyl ester (CFSE) Cell Proliferation Kit (Invitrogen; 1µM) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the chromatin and the 10% input were both reverse-crosslinked at 65°C for 3 hours with 10 μg of RNase A (ThermoFisher, EN0531) and 20 μg of thermoresistant proteinase K (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated glycoproteins were enriched by incubation for 3 hrs at 4°C on a rotating wheel with high affinity streptavidin agarose beads (ThermoFisher Scientific). Extensive washing was performed on a vacuum manifold ...
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bioRxiv - Neuroscience 2023Quote: ... To revive hiPSCs from cryopreservation cells were thawed by incubation for 2 minutes at 37°C and retrieved by centrifugation (200 x g for 3 minutes) prior to resuspension in Essential 8 (E8, (A1517001, Life Technologies) medium with 1’RevitaCell ...