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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... followed by ~2 – 3 drops of Prolong Gold Antifade Mountant (P36930, Thermo Fisher Scientific, Waltham, MA) and coverslipped ...
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bioRxiv - Genomics 2019Quote: ... Enriched libraries were then sequenced 2×50bp using SOLiD 3 sequencing chemistry (Applied Biosystems, Burlington, ON). A similar procedure was used to produce fragment libraries ...
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bioRxiv - Genomics 2021Quote: ... Samples were treated with 2–3 drops of Image-iT FX Signal Enhancer (Molecular Probes; 136933) for 15◻min with rotation at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 2-[6-(4’-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) from Molecular Probes® and Horse radish peroxidase (HRP ...
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bioRxiv - Physiology 2021Quote: 2-3 viable human slices were incubated with Fluo4-AM (6 μM, Invitrogen cat. No. F1221) for 1h in 3 mM HEPES buffer (125 mmol/l NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... were purchased from Click Chemistry Tools and Tris [(1-benzyl-1H-1, 2, 3-triazol-4-yl)methyl] amine from Fisher Scientific.
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bioRxiv - Molecular Biology 2021Quote: ... SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher) at room temperature.
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bioRxiv - Systems Biology 2022Quote: ... Peptides were trapped on precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and separated on an EASY-Spray column (ES903 ...
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bioRxiv - Cell Biology 2023Quote: ... All cells were passaged every 2-3 days using 0.05% Trypsin/EDTA (Gibco, Catalog no. 25300054). All cell lines were incubated at 37°C and maintained at 5% CO2.
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bioRxiv - Genomics 2023Quote: ... Cells were selected 3 days after transfection with puromycin (2 µg/mL) (Thermo Fisher Scientific, A1113803) or blasticidin (8 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... fed daily with growth factors and passaged every 2-3 days using TrypLE Express enzyme (GIBCO) to detach cells and trypsin inhibitor (GIBCO ...
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bioRxiv - Neuroscience 2023Quote: ... 11319718) were coated dropwise with 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate (DiI, 2 mg, Invitrogen, D282) dissolved in dichloromethane (400 ul ...
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bioRxiv - Immunology 2023Quote: ... The tissue was then cut into 2-3-cm segments and incubated in RPMI media (ThermoFisher) containing 5 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (ThermoFisher) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Peptides were trapped on precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and separated on an EASY-Spray column (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 and 24 hours later the plating medium was exchanged against NeurobasalTM containing 2% BL27 (Gibco) and 0,8 mM L-glutamine (all Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were prepared by incubating with a 2% solution of (3-aminopropyl)trimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell passaging was performed every 2–3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer.
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bioRxiv - Genetics 2024Quote: Testes from 2–3 day-old flies were dissected and transferred to Schneider’s insect medium (Gibco) with or without 50 ng/µl α-amanitin ...
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bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
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bioRxiv - Molecular Biology 2020Quote: Total RNAs from 7-d-old seedlings grown on 1/2 MS with or without 1 μM ABA were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) or Universal Plant Total RNA Extraction Kits (Spin-column)-I (BioTeke ...
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Biophysics 2020Quote: ... Subsequently, protein samples were washed once using Zeba™ Spin desalting columns (7 kDa MWCO, 2 mL) (Thermo Fisher Scientific, Waltham, MA, USA) equilibrated with degassed buffer A ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... For each sample 2 μg of solubilized silk protein was loaded into a NuPAGE™ 7% Tris-Acetate gel (EA03585BOX, Invitrogen, Waltham, MA) under reducing conditions ...
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bioRxiv - Biochemistry 2023Quote: ... and transferred onto a 0.45 μm nitrocellulose membrane for 7 min at 20 V with the i-Blot 2 (Thermo Fisher, Waltham, MA, USA). Blocking ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed 3 times for 15 min at 67°C in 1X Hybridization Stringency Wash Buffer (Thermo Fisher), developed ...
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bioRxiv - Microbiology 2020Quote: ... Forty cycles of amplification at 60 °C were conducted in a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Amplification curves were analyzed using QuantStudio Design & Analysis Software v1.5.1 ...
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bioRxiv - Cell Biology 2021Quote: ... and lifted for 3 min at 37°C using 5 mL of TrypLE Express (Invitrogen, Gibco, Cat no. #1260501). The appropriate number of cells were then spun down at 90 x g for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and lifted for 3 min at 37°C using 5 mL of TrypLE Express (Invitrogen, Gibco, Cat no. #1260501). The appropriate number of cells were then spun down at 90 x g for 10 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... a recodonized TgHypo cDNA sequence fused to a C-terminal 3×HA tag was synthesized by GeneArt (Life Technologies). This fragment was cloned into the BamHI and HindIII sites of a modified version of the parental plasmid p5RT70loxPKillerRedloxPYFP-HX3 ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... and incubated (95 °C, 20 min) before SDS-PAGE using 3–8 % Tris-Acetate protein gels (Thermo Fisher Scientific). After transfer onto PVDF membranes (iBlot 2 Dry Blotting System ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were cultured at 37°C with 5% CO2 for 3 days in RPMI-1640 medium (Thermo Fisher Scientific) supplemented 10% FCS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... boiled at 95°C for 5 min and loaded into a NuPAGE 3-8% Tris-Acetate Gel (Thermo Scientific) along with HiMark™ Pre-stained Protein Standard (Thermo Scientific LC5699) ...
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bioRxiv - Neuroscience 2023Quote: Samples were heated to 95°C for 3 minutes in loading buffer NuPAGE LDS Sample Buffer (4x) (Invitrogen NP0008) with 5% β-Mercaptoethanol then loaded on 4-20% Criterion TGX Precast Midi protein gels (Bio-Rad #5671094) ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... and inflated using a needle and syringe with warm (42°C) 3% low-melting point agarose (ThermoFisher #BP165-25) diluted in DMEM-H (Gibco #11965-092) ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons 13-15 days-in-vitro (DIV) were transfected with plasmid (2 h, 2-3 µg per dish) with Lipofectamine 2000 (Invitrogen, #52887, minimum 24 h). For pH imaging experiments ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were subsequently incubated for 2 hours at 4°C with Streptavidin Dynabeads (Invitrogen M-280), and beads were successively washed with RIPA (2 times) ...
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bioRxiv - Genetics 2021Quote: ... The samples were then cooled to 37 °C and 2 μL of RNase Cocktail (ThermoFisher, AM2286) was added to each sample and the sample was mixed well by pipetting ...
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bioRxiv - Genomics 2019Quote: ... Hi-C DNA bound to capture RNA was isolated using a DynaMag-2 magnet (Life Technologies). Washes (15 min in 500 μl wash buffer I at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... whereas BE(2)-C and HeLa cell lines were grown in Gibco DMEM medium (ThermoFisher, 11995073) with 10% FBS and 100U/mL PenStrep ...
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bioRxiv - Cell Biology 2019Quote: ... denatured at 90 °C for 2 min and separated on a 10% NuPAGE gel (ThermoFisher Scientific) at 120 V for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... and then flash-frozen in dry-ice-chilled 2-methylbutane (−40 °C) (#03551-4, Fisher Scientific). Regions of interest were collected from these slices using a 1 mm biopsy micropunch (#15110-10 ...
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bioRxiv - Cell Biology 2021Quote: ... Jackson) at 37 °C for 2 h and the Hoechst 33342 (1:200, H3570, Life Technologies) to label DNA ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and incubated with 40ul washed Dynabeads Protein G (Invitrogen 10003D) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... for 15 min at 37°C before 2 washes in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (University of California ...