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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... pH 6.8) for 5 min at 95° C and loaded to NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (Invitrogen, #NP0323BOX). After electrophoresis ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was precipitated with ice-cold isopropanol and 3M sodium acetate (pH 5) at 4°C before RNA pellets were resuspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was precipitated with ice-cold isopropanol and 3 M sodium acetate (pH 5) at 4°C before RNA pellets were suspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged (13,000 × g for 5 min at 4°C) and supernatants are precleared with 10 μl protein G beads (Invitrogen; 1004D) at 4°C for 1 h ...
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bioRxiv - Physiology 2022Quote: HEK293-H cells were maintained in suspension at 37°C with 5% CO2 in CD293 medium supplemented with 4 mM GlutaMAX (Invitrogen). For imaging and electrophysiology ...
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bioRxiv - Bioengineering 2024Quote: ... MOLT-4 cells were maintained at 37°C with 5% CO2 in suspension culture in a complete growth medium (Gibco’s RPMI 1640 with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then incubated at 95°C during 5 minutes and separated by SDS-PAGE using 4-12% Bis-Tris gels (Invitrogen), stained for 1 hour using BioSafe-Coomassie stain (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 μL of sample was applied and the grid was blotted for 5 seconds at 4 °C and 95% relative humidity then plunge-frozen with liquid ethane using a Vitrobot (ThermoFisher). Samples were clipped and screened by Glacios transmission electron microscope before 5,276 movies were collected on a Titan Krios transmission electron microscope at the Center for Electron Microscopy and Analysis (CEMAS ...
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bioRxiv - Genetics 2024Quote: ... Samples were boiled at 95°C for 5 min before loading (60 µg) on 4-12% Bis-Tris gel (ThermoFisher) and ran with 1X MES buffer (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... samples were mixed using either standard SDS Lämmli sample buffer and heated at 80°C for 5 min prior to loading onto gels (Novex Nupage 4-12% Bis- Tris gels, 1mm, 15 well, Invitrogen) and ran with Novex Nupage MES-SDS buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Then the samples were heated at 95°C for 5 min and loaded onto a pre-cast Polyacrylamide 4-12% gradient gel (Bolt, Invitrogen). The proteins were then transferred onto a nitrocellulose membrane and then blocked with 10% Blotto (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated at 95°C for 5 minutes before loading on a 4-12% gradient Bis-Tris gel (Invitrogen). MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged at 500 g for 5 minutes at 4°C and red blood cells were hemolyzed using ACK buffer (Gibco). White blood cells were then incubated with 1:1000 Fc Block (AbLab ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... blocked in 5% donkey serum and incubated overnight at 4°C with a rabbit anti-Rbm20 antibody (PA5-53068, Invitrogen) at 0.5 μg/ml ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The first half was treated with 1x SDS loading dye for protein visualisation and incubated at 70°C for 5 minutes and loaded onto a WedgeWell Tris Glycine 4-12% gradient gel (ThermoFisher). Coomassie Brilliant Blue (CBB ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspension was incubated with Fc block antibodies for 5 minutes at 4°C and then stained with Thy1.2-FITC antibody and IgG2a isotype antibody (Thermo fisher) as a negative control for 30 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were collected (5 minutes, 1,000 rpm, 4°C) and RNA was isolated using the PureLink™ RNA Mini Kit (Ambion). RNA concentration was measured using NanoDrop 8000 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... the mix was cooled down to 4°C and supplemented with 1 μl of a solution containing 4 U Terminal Transferase (Thermo Scientific), 0.1 μl 10X NEB 4 buffer (NEB) ...
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bioRxiv - Genetics 2019Quote: ... Brain samples were homogenized at 4°C in 1 mL of TRIzol reagent (Life Technologies) using ceramic beads and automated homogenizer (PreCellys) ...
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bioRxiv - Cell Biology 2019Quote: ... overnight at 4°C followed by incubation with 1:200 Alexa-Fluor secondary antibodies (Invitrogen) for 2 hours at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM ThDP) at 4 °C using a 20 kDa MWCO dialysis cassette (Thermo Scientific).
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bioRxiv - Biochemistry 2020Quote: ... overnight at 4°C followed by Alexa 488 (1:300, ThermoFisher Scientific, Breda, the Netherlands) for 2 hours at RT ...
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bioRxiv - Genetics 2022Quote: ... eyecups were incubated overnight at 4°C with Alexa Fluor 488 Phalloidin (1:500; Invitrogen) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... overnight at 4° C and Alexa Fluor 488 anti-rabbit secondary antibody (Invitrogen; 1:500) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... for 1 hour at 4°C and analyzed on an Attune Flow Cytometer (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Stained lenses were washed three times in 4°C Medium 199 and then 200 μL 4°C PBS (Gibco, MA) was added to each well prior to measuring DHR fluorescence intensity at Ex/Em of 507/529 nm and Hoescht 33342 at Ex/Em of 360/460 nm with a microplate reader (Spectramax M3 ...
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bioRxiv - Bioengineering 2022Quote: Cell apoptosis was evaluated with CellEvent® Caspase 3/7 Green (Thermo Fisher, UK), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CellEvent Caspase-3/7 green flow cytometry assay kit was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with CellEvent caspase-3/7 green detection reagent (ThermoFisher cat # C10423) according to manufacturer’s instructions at a final concentration of 8 µM ...
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bioRxiv - Neuroscience 2021Quote: ... media was changed to Retinal Differentiation Media (RDM) [7:3 DMEM (Gibco 11965-118):F12 (Gibco #11765-054) ...
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bioRxiv - Cell Biology 2023Quote: ... flow cytometric apoptosis quantification was performed using the CellEvent Caspase 3/7 kit (ThermoFisher) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2023Quote: ... or CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Fisher Scientific) to label apoptotic cells (Caspase-3/7 activity-positive and SytoxAADvanced-negative ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptotic cells were detected with CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (1:500) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 6µM CellEvent Caspase-3/7 Green Detection Reagent (Thermofisher, #C10423, green fluorescence). Cancer cell lines (IGR-Heu and IGR-Pub ...
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bioRxiv - Cell Biology 2023Quote: ... the medium was switched to SFRM (DMEM/F12 [7:3] supplemented with B27 (Invitrogen), 2 mM L-glutamine).
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Immunology 2024Quote: ... we injected the mice intraperitoneally with 4 mg/ml of 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Waltham, MA) with the goal of labeling circulating monocytes to assess macrophage retention among groups ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10−5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10-5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Immunology 2019Quote: ... and frozen down in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Frozen PBMCs were stored at −80°C before shipping on dry ice to Benaroya Research Institute for further processing and stimulation as described above.
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bioRxiv - Immunology 2019Quote: ... one half being used immediately and the other half frozen in 7% DMSO in fetal bovine serum and cooled at −1°C/min in a freezing container (Nalgene, ThermoFisher Scientific). Freshly isolated PBMCs were plated in 96 deep-well plates (Greiner Bio-One ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear staining was performed using 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng ml−1; Molecular Probes). Sections were cover-slipped using ProLong Glass mounting agent (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (1:10,000, Invitrogen) for 3 min and eventually coverslips were mounted with Dako mounting kit (Fluka) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mmol/l 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Gibco, Germany #15630056), 2.5 ng/ml human fibroblast growth factor-basic (hFGF2 ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI was used (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10 000, Invitrogen).