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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... spun down at 1,000 × g for 10 min and pooled in equal proportions in Grace’s medium (Grace’s insect media [Sigma-Aldrich] with 4.2 mM NaHCO3, 10% FCS [Gibco], 1 × Penicillin/Streptomycin [Gibco] and 1 × BME Vitamins [Sigma-Aldrich] and adjusted to pH 5.25 ...
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bioRxiv - Microbiology 2020Quote: ... spun down at 1,000 × g for 10 min and pooled in equal proportions in Grace’s medium (Grace’s insect media [Sigma-Aldrich] with 4.2 mM NaHCO3, 10% FCS [Gibco] ...
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bioRxiv - Genetics 2023Quote: ... the larvae were briefly washed in 1X PBS then immediately dissected in 1X supplemented Grace’s insect media (Gibco). Salivary glands were mounted in 1X supplemented Grace’s insect media (Gibco ...
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bioRxiv - Immunology 2023Quote: ... D1-3 and D4-5 proteins were fluorescently labelled with Alexa Fluor™ 594 or 488 Microscale Protein Labeling Kits (Invitrogen™, A30008 or A30006) as described [15] ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with Cell Stimulation Cocktail plus protein transport inhibitors (ThermoFisher) at 37 °C for 5 hours followed by Zombie NIR and cell surface antigen staining ...
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bioRxiv - Biochemistry 2020Quote: Cultivated Jurkat cells were collected and washed 3 times in flow buffer (PBS without Ca/Mg, 3% FBS, Gibco). Cell concentration was measured ...
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bioRxiv - Cancer Biology 2020Quote: ... TIC-enriching 3-D cultures (3-D) were maintained in stem cell media: DMEM:F12 (+ L-glutamine, + 15 mM HEPES) (Gibco) supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from control (n=3) and model (n=3) Huh7 cells were isolated by TRIZOL reagent (Thermo Scientific), and the RNA concentration ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration of cell lysate was measured using the Coomassie protein assay kit (Thermo Scientific). Proteins were separated on SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... The cultured cells were treated to extract proteins using a protein extraction kit (Thermo Fisher), with bovine serum albumin as the standard ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Plasma membrane protein was isolated using Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration of cell lysates was measured using Pierce BCA Protein Assay (Thermo Fisher Scientific), following supplier’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cell lysate protein concentrations were measured using Pierce 660 nm Protein Assay Reagent (ThermoFisher Scientific) on a multimode plate reader (Infinite M200 PRO ...
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Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... The protein concentrations in cell lysates were quantified using Pierce BCA Protein Assay Kit (ThermoFisher). Equal amounts (25 μg ...
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bioRxiv - Cell Biology 2019Quote: ... The protein concentrations of cell extracts were determined with Coomassie Protein Assay Reagent (Thermo Scientific) with bovine serum albumin (BSA ...
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bioRxiv - Physiology 2021Quote: ... Protein concentrations of cell lysates were determined by Pierce BCA protein assay (Thermo Fisher Scientific). Equal amounts of cell-lysate protein and equal volumes of supernatant were resolved on 12% NuPage Bis-Tris gels and transferred to nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysate protein concentration was measured using Pierce BCA Protein Assay Kit (ThermoFisher Scientific, 23225) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration of cell lysates was determined using Pierce BCA Protein Assay (ThermoFisher Scientific, 23225). Total protein (40-60μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration from whole-cell extracts were determined using the Bradford Protein Assay (ThermoFisher Scientific). Equal amounts of protein (10-40 μg/lane ...
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bioRxiv - Immunology 2024Quote: Protein cell lysates together with pre-stained protein ladder (Fisher Scientific, Cat. No. BP3603-500), were resolved on 10% SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: Cell Lysis Buffer (CST) was used to extract protein lysates and the Subcellular Protein Fractionation Kit for Cultured Cells (ThermoFisher Scientific) was used for fractionation according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
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bioRxiv - Molecular Biology 2021Quote: ... We used TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher Scientific) to set up 10 μl (half volume ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The virus samples were treated with 300 units of RNase I (Ambion) for 30 min followed by SUPERaseI RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... 2.5mL virus with 10μg/mL polybrene (Thermo Fisher Scientific TR-1003-G) was added to 5×105 cells plated in 6-well dishes ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrated virus was filtered using 0.45um syringe filters (ThermoFisher; Cat#: 725-2545) and immediately added to cultured OSE in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... containing 7,5 μl 4xTaqMan Fast Virus 1-Step Master Mix (Life Technologies) and 1 μl Primer/Probe mixture29,30 ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were resuspended in CO2-independent media (ThermoFisher, Cat# 18045088), aliquoted ...
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bioRxiv - Immunology 2019Quote: ... purified virus was immobilized on 96-well maxisorp microtiter plates overnight (Nunc). Wells were blocked with 0.05% PBST [0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... c-MYC and KLF4 in CytoTune Sendai virus vectors (Thermo Fisher Scientific) (Sontag et al. ...
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bioRxiv - Biophysics 2022Quote: ... X-31 influenza virus was diluted in Opti-MEM (Gibco, cat # 11058021) at a ratio of 1:10000 to add 250 µL into each well (25) ...
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bioRxiv - Microbiology 2022Quote: ... the reactions with Taqman Fast Virus 1-step master mix (ThermoFisher scientific) were half of the recommended 20 μl volume ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-containing pellet was resuspended in 200 μL of DPBS (ThermoFisher) and stored at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... 10-fold dilutions of each virus in DMEM + 1% antibiotic/antimycotic (Gibco) were added to a monolayer Vero E6-TMPRSS2-T2A-ACE2 cells for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was performed with Moloney murine leukemia virus reverse transcriptase (Invitrogen) at 38°C for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) (3-8% gradient gels, Invitrogen) and subsequently transferred onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was dialyzed in a Slide-a-Lyzer cassette with a 3 kDa cutoff (Thermo Scientific) in step-wise gradient of NaCl and imidazole before final dialysis for 16-18h in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and 40 µg of antibody was bound to 3 mg of protein G Dynabeads (Thermo Fisher Scientific) in 400 µL of PBS buffer containing 0.2% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3, ThermoFisher, Waltham, MA). Assays were performed according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Processed proteins samples were run on 3–8% NuPAGE Tris-Acetate 1.5mM pre-cast gels (ThermoFisher, EA0378BOX) and primary antibodies used were ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were loaded into 3-8% Tris-Acetate mini gels (Thermo Fisher Scientific, cat no. EA03785BOX), separated electrophoretically ...
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bioRxiv - Immunology 2022Quote: Recombinant tri-S protein was heat-denatured at 100°C for 3 min in loading buffer (Invitrogen) containing 1X sample reducing agent (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitation was performed at 4°C for 3 hr with 50 µl protein G dynabeads (Thermo Fisher). Beads were washed five times with ice-cold Wash buffer 1 (50 mM Hepes-KOH (pH7.6) ...
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bioRxiv - Systems Biology 2019Quote: ... 3-30μg protein per well was separated using NuPAGE precast 4-12% polyacrylamide gel system (ThermoFisher Scientific). Immunoblotting was carried out using an X-Cell II blot module (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal protein lysates were run on Novex 3– 8% Tris-acetate 15 Well Mini Gels (EA03785BOX, Invitrogen) and transferred to Immobilon-P membranes (IPVH00010 ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal protein lysates were run on Novex 3–8% Tris-acetate 15 Well Mini Gels (EA03785BOX, Invitrogen) and transferred to Immobilon-P membranes (IPVH00010 ...
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bioRxiv - Biochemistry 2022Quote: ... following the separation of protein complexes on 3–12% precast Bis-Tris Native PAGE gels (Life Technologies), the proteins were transferred to polyvinylidene difluoride (PVDF ...