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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC; Thermo Fisher Scientific), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 min Samples were run down a Novex 4–12% Tris-glycine gel (Invitrogen) and transferred onto a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 4-5 ×106 P25 TCR-Tg CD4+ T cells (CD45.1) were labeled with CellTrace (Invitrogen) and adoptively transferred by tail vein injection in 100 µL of sterile PBS 24 hours (h ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were split every 4–5 days using 0.5 mM EDTA (Invitrogen, 15575-020). hiPS cell clones/lines were also generated using retroviruses or non-integrating episomal vectors from fibroblasts (Bhaduri et al. ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... Slides were counterstained with 5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific) for 10 min at room temperature and washed with 1 × PBST (0.2% Triton-X 100 ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min and loaded onto 4%–12% Nupage Bis-Tris gels (Novex/Life Technologies). Proteins were transferred using the iBlot2 (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 4-5 days by treatment with StemPro Accutase (ThermoFisher, Cat#A1110501), stored in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the 11.5 μL RNA were supplemented with 4 μL 5× first strand buffer (ThermoFisher Scientific), 1 μL 10 μM reverse primer ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 minutes and loaded onto NuPAGE 4-12% Bis-Tris Midi Gels (Invitrogen, WG1403BOX) and run at 140V for approximately 1.5 hrs ...
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bioRxiv - Microbiology 2024Quote: ... 20 mM glucose) before being loaded with 5 µM Fluo-4 AM (Invitrogen, Waltham, MA) with 1% PowerLoad Concentrate ...
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bioRxiv - Immunology 2024Quote: ... for 5 minutes at 4°C before quenching in 1x Phosphate Buffered Saline (PBS; Gibco). Cell viability was quantified by staining with Acridine Orange/Propidium Iodide at a 1:1 dilution in a Cellometer slide before reading on an Auto2000 Cellometer (Nexcelom Biosciences ...
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bioRxiv - Bioengineering 2024Quote: ... adding 5 mM GMP and 4 U/µL RNaseOUTTM recombinant ribonuclease inhibitor (Thermo Fisher Scientific) to the transcription reaction ...
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bioRxiv - Neuroscience 2024Quote: ... Each iWAT and eWAT depot received 4–5 μL of 0.1% CTB-488 (Invitrogen C34775) or CTB-647 (Invitrogen C34778 ...
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bioRxiv - Cell Biology 2024Quote: ... On DIV 4-5 medium was exchanged for phenol red-free Neurobasal medium (Thermo Fisher) supplemented with GlutaMAX 1x (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: hESCs were routinely passaged every 4–5 days by dissociating in Accutase (ThermoFisher Scientific, A1110501) for 3 minutes at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 5-10µg of lysate was ran on 4-12% NuPAGE Bis-Tris Gels (ThermoFisher), transferred to PVDF membranes ...
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bioRxiv - Immunology 2022Quote: To analyze cell morphology 5×104 cells were resuspended in 100mL PBS and cytocentrifuged onto slides at 350g for 5 minutes (Thermo Scientific, Cytospin 4) before fixation in methanol for 15 minutes and staining with May-Grünwald’s (VWR Chemicals ...
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bioRxiv - Molecular Biology 2021Quote: ... or amino acids (MEM amino acids solution; Gibco, Waltham, MA, USA).
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bioRxiv - Biochemistry 2024Quote: ... Except for lauric acid (Henkel) and 1,18-Octadecanedicarboxylic acid (Thermofisher Scientific), lipids were from Merck ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The dried peptide samples were re-dissolved in 0.1% formic acid and loaded onto a C18 column (100 μm× 20 mm, 3 μm, Thermo Fisher Scientific, USA) then separated on a C18 analytical column (150 μm × 120 mm ...
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bioRxiv - Microbiology 2021Quote: Challenged A549 cells were lysed with modified RIPA buffer (Supplementary Data 3) and protein concentration was determined using a bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific). Phospho-p38 (p-p38) ...
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bioRxiv - Genomics 2021Quote: ... and then blocked for 1 hour with 1 ml of blocking buffer (wash buffer containing 3% fatty acid free BSA (Thermo Fisher, 126609)) ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were reconstituted in LC-MS injection buffer (3% acetonitrile, 0.1% formic acid) and quantified by Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, Product #23290).
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bioRxiv - Molecular Biology 2023Quote: ... The resulting tryptic peptides were extracted by adding 50% digest dilution buffer (60 mM AB solution with 3% formic acid) and injected into a HPLC system coupled to a hybrid LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific) via a nano-electrospray ion source ...
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bioRxiv - Microbiology 2022Quote: ... on the cell membrane potential was assessed by using the bis-(1,3-dibutylbarbituric acid) trimethine oxonol - DiBAC4(3) reagent (Invitrogen, Carlsbad, CA, USA) according to Veerana et al. ...
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bioRxiv - Microbiology 2022Quote: ... Roughly 1/3 of the plate (2-3 loopfuls) were added to 400 µl 1x Tris-Ethylenediaminetetraacetic acid (EDTA) pH 7.5 (Thermo Scientific, Waltham, USA) and heated at 80 ºC for 30 min ...
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bioRxiv - Biochemistry 2023Quote: Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Bis-(1,3-diethylthiobarbituric acid) trimethine oxonol (DiSBAC, relative polarization) and CoroNa green, acetoxymethyl ester (CoroNa, sodium ions) were purchased from Invitrogen (Waltham, MA).
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bioRxiv - Cancer Biology 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Immunology 2022Quote: ... and under a 12-hour day/night photoperiod schedule (Percival Scientific, IA, USA) with 5% dextrose (w/v) and 0.05% para-Aminobenzoic acid (w/v) (Thermo Fisher). Mosquito oocysts were checked at 10-days post-infection ...
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bioRxiv - Neuroscience 2020Quote: ... MEF were grown as monolayers at 37°C with 5% CO2 in DMEM supplemented with 10% serum and MEM non-essential amino acids (Gibco), or with 5% delipidated fetal bovine serum and 35 μM oleic acid ...
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bioRxiv - Biochemistry 2022Quote: ... the gel was rinsed in Milli-Q water for 5 minutes and stained for 20 minutes with 1x SYBR gold nucleic acid stain (Invitrogen) in 1x TBE ...
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bioRxiv - Genetics 2022Quote: ... as well as fibroblasts and SH-SY5Y cells treated for 5 hours with 100 µM oleic acid (OA) following over-night starvation in Opti-MEM (ThermoFisher). OA is a potent inducer of lipid droplet formation (55) ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: Total RNAs of patients were extracted from either FFPE (Formalin-fixed paraffin-embedded) or frozen sections (5 x 20µ) using RecoverAll Total Nucleic Acid Isolation (Ambion) or Trizol (Ambion ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were resuspended in 2% acetonitrile in 0.1% formic acid and loaded at 5 μL/minute onto a PepMap C18 trap column (Thermo Scientific; 5 μm 100 Å particle size ...
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bioRxiv - Immunology 2024Quote: ... permeabilised with 0.5% Triton x-100 and OPP incorporation into newly synthesised peptides was measured by conjugating with Alexa647-azide via a Click-IT chemistry reaction (2 µM Alexa-647-azide, 2 mM CuSO4, 5 mM ascorbic acid in PBS) (Invitrogen) and assessing fluorescence by flow cytometry.
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bioRxiv - Plant Biology 2021Quote: ... the peptides were dissolved in 4 μL of 0.2% formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15 cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 AM dye was dissolved in a mixture of dimethyl sulfoxide (DMSO) and 20% pluronic acid (Invitrogen, Burlington, Ontario, Canada) to a 0.5 mM stock solution and kept frozen until used ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated overnight in a humidified chamber at 4 °C in TBS-T with 1% Fatty acid free-BSA (Fisher Scientific) with anti-mouse/human antibodies ...
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bioRxiv - Cell Biology 2023Quote: Peptides were dissolved in 4 μL 0.2% (v/v) formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15-cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...