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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... exponentially growing cells in suspension were diluted to 3×109 and 5 µl spotted on 1.5% agar (Gibco) supplemented with 0.5% CTT and immediately covered with a coverslide ...
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bioRxiv - Developmental Biology 2023Quote: ... rtf1-e3 gRNA (target sequence: 5’-GGAAGAAGGGGAAACCGAGCAA-3’) was synthesized using the MEGAshortscript T7 Transcription Kit (ThermoFisher Scientific) and purified using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 x 5 min with PBS and incubated with Alexa Fluor-488 goat anti human secondary (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (Thermo Scientific SuperSignal West) and imaged using a BioRad Chemidoc.
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bioRxiv - Neuroscience 2023Quote: ... and then washed with PBS 3 x 5 min and mounted with ProLong Gold Antifade Mountant (ThermoFisher, P36930). Mounted coverslips were then imaged on a Zeiss Axio Observer-Z1 widefield microscope equipped with a Zeiss Plan-Apochromat 63x / 1.4 Oil objective ...
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bioRxiv - Cell Biology 2023Quote: The full length Aff3ir mRNA was cloned using commercially available 5’-RACE and 3’-RACE kits (ThermoFisher Scientific) according to the protocols provided ...
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bioRxiv - Microbiology 2022Quote: ... Vero-E6 cells and Calu-3 were treated with 5 μg/ml or 1 μg/ml puromycin (Gibco), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were passaged in clumps at ∼ 70% confluency every 3 – 5 days by dissociation with Versene (Life Technologies) at typical ratios between 1:6 and 1:12 ...
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bioRxiv - Physiology 2024Quote: ... 1x 3–5 min with PBS + 1ng/mL DAPI and mounted in ProLong Gold antifade reagent (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed 3 × 5 min in PBS and embedded into ProLong Diamond Antifade Mountant (Thermo Fisher Scientific). For control experiments ...
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bioRxiv - Synthetic Biology 2024Quote: ... Passaging was performed by brief (3-5 minute) treatment with 0.5 mM EDTA and 0.25X TRYPLE Express (Gibco) in phosphate-buFered saline (PBS) ...
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RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid LeukemiabioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from 3-5 × 106 cultured MOLM-13 cells using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The same test was done in parallel to assess the cell viability using MTT at 5 ug/mL (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Invitrogen). Sixteen sites per well were acquired using a 20x Olympus objective plan fluorite NA 0.45 (1320517 ...
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bioRxiv - Cell Biology 2024Quote: ... We performed MTT (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) assay (cat no. V13154; Thermo Fisher) for determining cell viability after different tunicamycin treatments as per the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... Cryo-STET data were collected using the STEM Tomography software (Fig. 2A, Fig. 3, Fig. 5; Thermo Fisher) or SerialEM (Fig ...
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bioRxiv - Evolutionary Biology 2024Quote: ... intestinalis was used for 3’RACE and 5’RACE experiments using FirstChoice™ RLM-RACE Kit (Invitrogen, AM1700) following the manufacturer’s protocol and with the following CiTCF-specific primers ...
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bioRxiv - Cell Biology 2024Quote: ... as such: position (636-653 bp) 5’ aCAcTAtCAgAGgGAtAT 3’ (modified region corresponding to siRNA from Thermo Fisher s38909) and position (1235-1252 bp ...
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bioRxiv - Cell Biology 2024Quote: ... as such: position (111-131 bp) 5’- GAaTCgAGtCTaGAgTCtAgc-3’ (modified region corresponding to siRNA from Thermo Fisher s226949) and position (607-627 bp ...
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bioRxiv - Cell Biology 2024Quote: ... as such: position (1411-1431 bp) 5’ aGaAAaCTgCTgAGgGTgTTa 3’ (modified region corresponding to siRNA from Thermo Fisher s228499) and position (2164-2187 bp ...
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bioRxiv - Cell Biology 2020Quote: ... BN-PAGE was performed using NativePAGE 3-12% or 4-16% Bis-Tris gels and associated running buffer (Invitrogen). The cathode buffer was supplemented with 0.002% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Excess liquid was blotted for 3 seconds with a force of -4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected and stimulated with a HL-3 solution of 90 mM K+/0.5 mM Ca2+/4 μM fixable FM1-43 (Invitrogen) for 1 min ...
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bioRxiv - Genomics 2020Quote: ... Two TaqMan probes targeting the MLLT3 exon 3-4 and exon 9-10 splice junctions (ThermoFisher, Hs00971092_m1 and Hs00971099_m1) were used with cDNA diluted 1:5 in dH2O to perform qPCR for six replicates per sample on an Applied Biosystems Quant Studio 6 Flex ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781; 1µg/ml) labeling was added from the second day to show the whole brain morphology ...
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bioRxiv - Synthetic Biology 2021Quote: N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (NBD-PE) was supplied by Thermo Fisher Scientific Inc ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then stained with 3 mL of overlay containing a 1:1 mixture of 1.2% oxoid agar with 4% neutral red (Gibco) and 2X DMEM with 2% (vol/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were subcultured every 3-4 days as follows: cells were washed with phosphate buffered saline (PBS, Life Technologies) and then incubated with trypsin (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... incubated for 10 min in the dark at room temperature with FM 4-64FX (3 μg ml-1) (ThermoFisher). Cells were applied to a 5% agarose pad containing M9 minimal media with glucose (0.4%) ...
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bioRxiv - Cancer Biology 2022Quote: ... RT reactions were performed with 3-4 µg total RNA using oligo-dT and SuperScript III RT (ThermoFisher Scientific). QPCR was performed as previously described [20] ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Genetics 2022Quote: Immunoprecipitations were performed using 1 μg of Anti-Trimethyl-Histone-3-Lys-4 (-Thermo Fisher Scientific; catalog# PA5-17420) overnight at 4 ◦ C ...
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bioRxiv - Molecular Biology 2020Quote: ... heated at 95°C for 3 min and seprated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to an PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... After 3 days the overlay was removed and cell monolayers were fixed with 4% formaldehyde (28906, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine) (Invitrogen, D3521) together with 5 µM delipidated BSA (as a delivery carrier ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCs cultured in serum-free ESGRO medium or serum-containing M10 medium were incubated with 5 µM BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine) (Invitrogen, D3522) or 5 µM BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine ...
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bioRxiv - Biochemistry 2023Quote: ... BODIPY-CE (cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3- dodecanoate) was obtained from Life Technologies. Lipids (with the exception of NBD-LPC ...
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bioRxiv - Physiology 2022Quote: ... Cells were fixed with ice-cold 4% paraformaldehyde solution and cell nuclei were stained with To-Pro-3 (Invitrogen). Samples were mounted with Mowiol (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... SDS-PAGE was carried out on either NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in Table S5.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged every 3-4 days during the log phase of growth with Trypsin/EDTA solution (Gibco, R001100) following a PBS wash ...
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bioRxiv - Genomics 2023Quote: ... 3-4 days post-nucleofection (depending on cell confluency) selection was started using 400 ng/mL Puromycin (ThermoFisher A1113802) in FM (PFM) ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0 NBD) were purchased from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: 100 to 150 3-dpf zebrafish larvae were bathed in Yo-Pro-1 (4 µM, 30 min) (Invitrogen, Y3603), then washed 3 times in blue water to label lateral line hair cells ...
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bioRxiv - Neuroscience 2022Quote: ... The craniotomy was then sealed with two glass coverslips (3 mm and 4 mm diameter, Thermo Fisher Scientific, UK) glued to one another using optical adhesive (Norland ...
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bioRxiv - Biochemistry 2022Quote: ... Acly-/- MEFs were maintained at <80% confluence (passaged every 3-4 days) in high glucose DMEM (Gibco #11965-084) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells at passage 3-4 were seeded in to black walled clear bottomed 96 well plates (Pierce, ThermoFisher Scientific) at a density of 7,500 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc ...
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bioRxiv - Immunology 2023Quote: ... and packaging vector psPAX2 were mixed in a 4:3:1 ratio in OPTI-MEM (Thermo Fisher Scientific, 31985070) and PEI (Polysciences ...