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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... slices were transferred into a sealed 1.5 ml tube containing 5 µM Fluo-4AM (Molecular Probes, 50% DMSO, 50% Pluronic Acid) diluted in oxygenated modified artificial cerebrospinal fluid (mACSF ...
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bioRxiv - Cancer Biology 2024Quote: ... Each sample was then resuspended in a volume of sample LC/MS loading solvent [5% formic acid (Optima grade, ThermoFisher Scientific) and 4% acetonitrile (both water and acetonitrile were LC-MS-grade ...
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bioRxiv - Cancer Biology 2024Quote: ... in the extracellular domain of the CaSR at a concentration of 5 μg/ml and counterstained with SYTO13 fluorescent nucleic acid dye (ThermoFisher, S7575) as previously described 33 ...
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bioRxiv - Immunology 2023Quote: ... A 5-fold excess of pre-warmed RPMI+ medium (RPMI-1640, 100 μM non-essential amino acids, 20 mM HEPES [Thermo Fisher Scientific] ...
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bioRxiv - Neuroscience 2022Quote: ... Eluted peptides were dried down overnight before resuspension in 5% formic acid ready for injection onto the Orbitrap Exploris (Thermo Fisher) mass spectrometer using data independent acquisition (DIA) ...
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bioRxiv - Cell Biology 2023Quote: For flowcytometric quantification of GFP signal in induced 3D7-DiCre pFIO/pFIO+ strains 2.5 µl iRBCs were washed once with RT 0.9% NaCl solution (B. Braun GmbH) and stained with 5 µM SYTO 61 Red Fluorescent Nucleic Acid stain (Thermo Fisher Scientific) for 1 h at 37°C in 0.9% NaCl solution and washed once again afterwards at RT ...
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bioRxiv - Cell Biology 2024Quote: ... TMB substrate (Thermo 34028) was added for approximately 5 minutes and reaction was stopped with sulfuric acid Stop solution (Invitrogen, SS04). Absorbance was immediately read at 450 nm ...
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bioRxiv - Microbiology 2024Quote: ... The dry pooled sample was resuspended in 1 ml of 5% formic acid and was desalted using the EasyPep Maxi Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were resuspended in 12 μL 0.5% formic acid and 5 μL of each sample was analyzed by nanoflow LC-MS/MS using an Orbitrap Exploris 480 (Thermo Fisher) mass spectrometer with an EASY-Spray source coupled to a Vanquish LC System (Thermo Fisher) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peptides were separated by a 140 min linear gradient from 5-30% ACN containing 0.05% acetic acid on an UltiMate 3000 UHPLC Nano (Thermo Fisher Scientific) coupled to Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were trapped at 20 μL/min in a loading solvent (2% ACN, 0.05% trifluoroacetic acid) on a 5 mm × 300 μm C18 PepMap cartridge pre-column (Thermo Fisher Scientific) for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were centrifuged for 5 min at 13 000 × g at 4°C and the concentration of protein was measured using a bicinchoninic acid assay (Thermo Fisher Scientific). Protein lysates were fractionated by SDS-PAGE and transferred onto PVDF membranes ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then spun down at 14,000 rpm at 4°C for 20 min and quantified using a bicinchoninic acid assay (Thermo Fisher Scientific, 23227). Protein lysates of 10–20 μg were added in with DTT (1 µL ...
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bioRxiv - Biochemistry 2021Quote: ... Cell debris was removed by centrifugation at 60,000g for 30 min at 4 °C and the supernatant was incubated with nickel-nitrilotriacetic acid (Ni-NTA) resin (HisPur Ni-NTA Resin; Thermo Fisher Scientific) for 1 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... for 90min effective gradient with 4-35% 0.1% formic acid in ACN on an Ultimate 3000 Nano liquid chromatography instrument (Thermo Scientific, Waltham, MA). The separated peptides flew to an Orbitrap Q-Exactive HF mass spectrometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Cell debris was cleared by centrifugation at 1000×g for 15 min at 4°C and protein concentration was determined by bicinchoninic acid (BCA) assay (ThermoFisher Scientific #23225). Protein lysates were resolved on Bolt™ 4-12% Bis-Tris Plus gels (ThermoFisher Scientific #NW04125BOX ...
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bioRxiv - Cell Biology 2023Quote: ... Extracts were then centrifuged at 10,000g for 10 min at 4°C and the protein concentration of the soluble fraction determined using the Bicinchoninic Acid (BCA) Assay (Thermo Fisher Scientific). Samples were denatured in 4X Laemmli sample buffer (BioRad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Brain extracts were clarified by centrifugation at 1,500x g for 5 min at 4 °C and then protein concentrations in the supernatant were determined using the bicinchoninic acid (BCA) assay (ThermoFisher Scientific #23227). Detergent-extracted brain homogenates were diluted to a concentration of 5 mg/mL in 1X detergent buffer and then incubated on ice for 20 min ...
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bioRxiv - Systems Biology 2023Quote: Samples were resuspended in 4% formic acid/2% acetonitrile solution and analyzed on an Q-Exactive Plus MS system (Thermo Fisher Scientific) equipped with an Easy nLC 1200 ultra-high pressure liquid chromatography system (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... is purchased from MakingCosmetics Inc. (USA). 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M solution, Cat. No. J16924-AP) is purchased from Thermo Scientific (USA). Silica beads (Cat ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 14,000xg for 15 minutes at 4°C and protein concentrations were determined using a standard Pierce™ bicinchoninic acid (BCA) assay (ThermoFisher, 23225). Lysate (30 µg ...
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bioRxiv - Physiology 2024Quote: ... Samples were centrifuged at 13 000 g for 15 min at 4°C and the protein concentration of each sample determined via Pierce Bicinchoninic Acid (BCA) assay (#23225, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were centrifuged at 15,000 x g at 4°C for 20 min and protein concentration was quantified using bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific). 10-25 µg of proteins were resolved in 8% SDS-PAGE gels before being transferred onto polyvinylidene (PVDF ...
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bioRxiv - Cell Biology 2021Quote: ... and a mix of amino-acids (1X MEM Non-Essential Amino Acids and 1X MEM Amino Acids, both manufactured by Gibco R). For strains and plasmid construction cells were grown in LB ...
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bioRxiv - Cell Biology 2024Quote: ... a mix of amino acids (1× MEM Non-Essential Amino Acids and 1× MEM Essential Amino Acids, both manufactured by Gibco™) was added with the glucose ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed on QuantStudio™ 3 and 5 Real-Time PCR System (Thermo Fisher Scientific, USA) using KAPA SYBR®Fast qPCR Master Mix (Kapa Biosystems).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed 3 x 5 min and incubated for 10 min with DAPI (1/2000, Invitrogen #D1306) diluted in PBT + 0.5% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... for 5 minutes followed by a wash with chilled PBS and blocked with 3% goat serum (Thermo Scientific) for 1h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 3-5 μl of purified γ-TuRC was visualized on an SDS-PAGE with SYPRO Ruby stain (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... hBMSCs (passage 3) were expanded at 37°C and 5% CO2 in expansion medium (Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... by incubating muscles twice for 45 min in preoxygenated Ringer containing 5 μM Rhod-3 AM (Life Technologies), 0.02 % Pluronic acid (Life Technologies ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Neuroscience 2020Quote: ... brain sections from 3- to 5-month-old mice were exposed to 2.5 µM MitoSOX Red (Molecular Probes), at 37°C for 30 min14 ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was sonicated for 3-5 mins using an ultrasonic cleaner (FS220, Thermo Fisher Scientific, Waltham, MA). The solution was diluted to 10 mL with 2% nitric acid ...
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bioRxiv - Cell Biology 2021Quote: ... 5 to 20 ng of RNA were amplified and labelled with the GeneChip 3’ IVT Pico Kit (Affymetrix) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 3 to 5 min in 100 μM Lysotracker-Red DND-99 (Life Technologies, Carlsbad, CA, USA)[49] and 1 μM Höchst 33342 (Thermo Fisher Scientific Inc ...
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bioRxiv - Genomics 2020Quote: ... slides were washed 3 times for 5 min with TNT and mounted with Prolong Gold (P36930, Life Technologies).
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bioRxiv - Plant Biology 2021Quote: ... The bands were detected by using SuperSignal pico/femto chemiluminescence kits (Thermo Scientific; #’s1859022/3 and 1859674/5).
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 and medium were changed every 3 days and cells were passaged with 0,05% trypsin-EDTA (Invitrogen) twice per week.
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bioRxiv - Genomics 2020Quote: ... Biotin labelled fragments which contained the 5’ and 3’ connecting region was enriched using Dynabeads M280 streptavidin (Invitrogen) and incubating at room temperature for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: 5’ - capped and 3’- polyadenylated hCas9-2xNLS mRNA was transcribed in vitro using mMessageMachine T7 (ThermoFisher, Cat# AM1344) and Poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA linker (5’-CACGACGCUCUUCCGAUCU-3’) was ligated to the polyA RNA with T4 RNA ligase (Thermo Scientific) to capture RNAs with a 5’ monophosphate (5’P) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Slides were again washed with PBS-/- 3×5 min and mounted using Prolong Gold Antifade Mountant (Thermo Scientific). Sections stained with secondary only antibodies were included to determine fluorescence threshold before image acquisition in each channel ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Sections were washed 3 times 5 minutes in PBS and mounted using ProLong Gold mouting media (Thermo Fisher) under glass cover slips.