Labshake search
Citations for Thermo Fisher :
9901 - 9950 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... and then with Alexa Fluor™ 594 streptavidin (3 hours – room temperature-Thermo Fisher). After extensive washing ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were washed once with PBS and incubated with 3 mL of TrypLE (Gibco) for 3-5 min or until cells lifted off from the plates with gentle tapping ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL NT-3 (PreproTech) and 1 µg/mL Laminin (Gibco, Thermo Fisher) plus DOX ...
-
bioRxiv - Immunology 2024Quote: ... tonsils were rinsed 3 x 5 minutes with 1X PBS (10010023, ThermoFisher Scientific, USA) without agitation ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL NT-3 (PreproTech) and 1 µg/mL Laminin (Gibco, Thermo Fisher) plus DOX ...
-
bioRxiv - Microbiology 2024Quote: ... and quantified using a cell counter (Invitrogen™ Countess™ 3 Automated Cell Counter) to analyze dead and live macrophages.
-
bioRxiv - Genomics 2024Quote: ... Samples (1E5 cells) were loaded and run on 3-8% Tris-Acetate Gels (ThermoFisher) in Running Buffer (1x NuPage Tris-Acetate Running Buffer ...
-
bioRxiv - Genomics 2024Quote: ... and a QuantStudio 3 or QuantStudio 5 Real-Time PCR instrument (Applied Biosystems™). Primers used for qPCR are listed in Suppl ...
-
bioRxiv - Immunology 2024Quote: ... using PEI (3:1 ratio) for transfection of 293F cells and ExpiFectamine (ThermoFisher, #A14525) for transfection of Expi-293F cells according to the manufacturer’s suggestion ...
-
bioRxiv - Immunology 2024Quote: ... cDNA was then subjected to real-time qPCR using a QuantStudio 3 (ThermoFisher Scientific) to quantify mRNA copies using PowerUP SYBR Green Master Mix (Applied Biosystems ...
-
bioRxiv - Immunology 2024Quote: ... The RT-qPCR was performed in a QuantStudio 3 (Applied Biosystems, Foster City, CA) using qScript XLT One-Step RT-qPCR ToughMix® ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were counted using the CountessTM 3 FL Automated Cell Counter (SN: AMQAF2000, Invitrogen) and strained using a 5-mL cell strainer tube (SN ...
-
bioRxiv - Biochemistry 2024Quote: ... Target DNA was similarly labeled at the 3′ end using Terminal Deoxynucleotidyl Transferase (Affymetrix) and [32P]cordycepin-5′-triphosphate ...
-
bioRxiv - Cell Biology 2024Quote: ... si-PRELID1: 5’-CCCGAAUCCCUAUAGCAAA-3’) were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific, 13778075). Assays were normally carried out 48 h after transfection ...
-
bioRxiv - Cell Biology 2023Quote: ... and viability was measured using an automated cell counter (Countess 3, Thermo Fisher Scientific). Single cell suspensions were processed using Chromium Single Cell 3’ Reagent Kits v3.1 (10x Genomics ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were resolved in 3-8 % NuPage Norvex tris-acetate Protein Gels (Life Technologies) and transferred to PVDF membranes (Life Technologies) ...
-
bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed on QuantStudio 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher) according to MIQE guidelines (Bustin et al ...
-
bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed on QuantStudio 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher) according to MIQE guidelines (Bustin et al ...
-
bioRxiv - Neuroscience 2022Quote: ... for 3 minutes and mounted with ProLong Gold antifade mounting medium (Thermo Scientific, #P36930). Fluorescence images were acquired with a SP8 confocal microscope (Leica ...
-
bioRxiv - Microbiology 2023Quote: ... Tris(hydroxymethyl)methyl-3-amino propane sulfonic acid (TAPS) was purchased from Acros Organics. Mal-PEG ...
-
bioRxiv - Biophysics 2023Quote: ... and then were loaded onto a 3%-12% Native-PAGE gel (Thermo Fisher Scientific) and stained with Coomassie blue.
-
bioRxiv - Neuroscience 2022Quote: ... followed by a 3 min permeabilization with 0.1% v/v Triton X-100 (ThermoFisher) solution in DPBS+ and two additional washes with Tween20 solution ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNP was added to 3×106 cells suspended in Opti-MEM medium (ThermoFisher Scientific). The cell mixture was electroporated using a NEPA21 electroporator (Bulldog Bio ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell counts were determined using a Countess 3 instrument (Cat No. AMQAX2000, Thermo Fisher), and 2,000,000 cells/well were plated in a 6-well plate in STEMdiff SMADi Neural Induction media ...
-
bioRxiv - Neuroscience 2023Quote: ... qPCR was performed with technical triplicates using a QuantStudio 3 qPCR machine (Applied Biosystems). qPCR target gene expression was normalized against the housekeeping gene Gapdh ...
-
bioRxiv - Microbiology 2023Quote: ... A 1:5 dilution of cDNA and custom primers were input into qPCR reactions with PowerUP SYBR Green Master Mix (Applied Biosystems).qRT-PCR was performed using a QuantStudio 3 Real Time PCR system (ThermoFisher Scientific) using cycling parameters as defined by the master mix instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from 3 replicates of colorectal cells using TRIzol reagent (Invitrogen). Reverse transcription of 1μg of RNA was performed according to the manufacturer’s instructions Reagents and detection systems were from Applied biosystems ...
-
bioRxiv - Genomics 2023Quote: ... Loss of enzymatic activity was confirmed by 3’-(p-aminophenyl) Fluorescein (APF) assay (ThermoFisher).
-
bioRxiv - Molecular Biology 2023Quote: ... and (3) OE-GNA13 or NC plasmids using Lipofectamine™ 2000 (Thermo Fisher Scientific) following the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 6µM CellEvent Caspase-3/7 Green Detection Reagent (Thermofisher, #C10423, green fluorescence). Cancer cell lines (IGR-Heu and IGR-Pub ...
-
bioRxiv - Synthetic Biology 2023Quote: ... cells were pelleted and washed 3 times with Hanks balanced salt solution (HBSS) (ThermoFisher). Cells were diluted back into HBSS and placed onto a 35-mm number 1.5 coverslip glass-bottom dish ...
-
bioRxiv - Developmental Biology 2023Quote: ... Samples were assayed using SYBRgreen on a QuantStudio 3 (Applied Biosystems by Thermofisher Scientific) with an extension temperature of 60 °C for 40 cycles ...
-
bioRxiv - Developmental Biology 2023Quote: ... Samples were assayed using SYBRgreen on a QuantStudio 3 (Applied Biosystems by Thermofisher Scientific) with an extension temperature of 60 °C for 40 cycles ...
-
bioRxiv - Cancer Biology 2023Quote: Quantitative polymerase chain reaction (qPCR) was performed using a QuantStudio 3 (Thermo Fisher Scientific) instrument ...
-
bioRxiv - Neuroscience 2023Quote: ... Brain sections were incubated with rabbit anti-Neuroligin-3 (1:250, Thermo Fisher Scientific) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... mixed with 3% w/v low melting point agarose (UltraPure, Invitrogen, Thermo Fisher Scientific) in F/2-Si medium to a final concentration of 0.3% ...
-
bioRxiv - Molecular Biology 2023Quote: ... with 10% FBS and split every 3-4 days using TrypLE Express (Thermo Scientific). Low-passage ...
-
bioRxiv - Genomics 2023Quote: ... 3 µL of 50 mg/mL BSA (Cat#AM2616, Thermo Fisher Scientific, Waltham, USA), 10 µL of 400 U/µL T4 DNA Ligase (Cat#M0202 ...
-
bioRxiv - Cell Biology 2023Quote: ... or pre-cast NuPAGE™ 3-8% Tris-Acetate gradient Gels (Thermo Scientific, EA0378BOX) and transferred to a polyvinylidene difluoride (PVDF ...
-
bioRxiv - Cancer Biology 2023Quote: ... (3) The Qiagen ATE buffer was replaced with Low TE buffer (Applied Biosystems 4389764), pre-heated to 55°C and applied to the column for ten minutes ...
-
bioRxiv - Microbiology 2023Quote: ... sonicated via a Sonic Dismembrator 100 (Fisher Scientific, setting 3, 15 s, 4 pulses), and incubated on ice for 1 hr after the addition of 2 μL benzonase (EMD Millipore) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Reactions were run on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) using the following standard thermal profile ...
-
bioRxiv - Cell Biology 2022Quote: ... transfected cells were selected with 3 μg/ml puromycin (#A11138-03, Thermo Fisher Scientific) 48 hours post-transfection ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell numbers were counted at passage using the Countess 3 automated cell counter (Invitrogen). Cell-line authentication (performance ...
-
bioRxiv - Cancer Biology 2023Quote: ... Transwell inserts were stained using Hema 3 Stat Pack (Thermo Fisher Scientific #123-869). Migration inserts were randomized prior to manual quantification and the outer 5% of the inserts were not included during quantification to reduce edge-effect bias ...
-
bioRxiv - Cancer Biology 2022Quote: ... Data analysis was carried out using the QuantStudio 3 software (Applied Biosystems, CA, USA) and the 2−ΔΔCT method (Livak and Schmittgen ...
-
bioRxiv - Genomics 2023Quote: ... use 10 μL for 2-3 ml of blood) or EDTA (0.5M EDTA, Gibco, cat ...
-
bioRxiv - Cell Biology 2023Quote: ... CCDC15 was depleted using an siRNA with sequence 5′-GCAGUACCUGAGACAUAGAtt-3′ (Ambion, Cat. # s36888). For depletion of POC5 ...
-
bioRxiv - Biophysics 2023Quote: ... 2’(3’)-O-(2,4,6-trinitrophenyl)-adenosine 5’-triphosphate (TNP-ATP) was purchased from Invitrogen as a trisodium salt and stored in the dark at -20 °C at 10 mg/ml_ ...
-
bioRxiv - Microbiology 2023Quote: ... qPCR reactions were run in a QuantStudio 3 real time PCR machine (Applied Biosystems). Gene expression was normalized to GAPDH (Hs01922876_u1 ...