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Citations for Thermo Fisher :
9701 - 9750 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Developmental Biology 2020Quote: ... 1/10th volumes of 3 M Sodium Acetate and 30 μg Glycoblue coprecipitant (Ambion).
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescent signals were acquired using QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific), CFX Connect Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... We ran RT-PCR in a QuantStudio 3 Real-Time PCR machine (Thermo Fisher) using continuous capture with a 0.025°C/s ramp ...
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bioRxiv - Cell Biology 2022Quote: PC-3 cells (ECACC 90112714) were maintained in RPMI medium (GIBCO, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: PC-3 cells (ECACC 90112714) were maintained in RPMI medium (GIBCO, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was biotinylated using the PierceTM RNA 3’ End Desthiobiotinylation Kit (Thermo Fisher Scientific). 50 pmol of RNA was incubated in T4 RNA ligase buffer at 16 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... or subcutaneous injection of 2.5% (w/v) Texas Red Dextran (3 kDa, neutral, Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... The library concentration was determined in a Qubit.3 Fluorometer (Catalog: Q33216, Life technologies using the Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Molecular Biology 2020Quote: ... zebrafish larvae at 3 dpf were incubated with LysoTracker Red DND-99 (Life Technologies) which contains fluorescent acidotropic probes for labeling and tracking of acidic organelles ...
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bioRxiv - Molecular Biology 2020Quote: ... using a top T (3□s) method as recommended by the manufacturer (Thermo Scientific). Andromeda implemented in MaxQuant (version ...
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bioRxiv - Molecular Biology 2021Quote: ... The Calu-3 cells were cultured with MEM (Thermo Fisher Scientific, Waltham, MA, USA) containing 20% (v/v ...
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bioRxiv - Developmental Biology 2022Quote: Testes were dissected from 1-3 day old males in SF-900 media (Gibco) then fixed in 4% formaldehyde diluted in PBS for 20-30 minutes and permeabilized in 1% PBT (triton x100 diluted in PBS ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Samples were separated on a NuPage 3-8% Tris-Acetate gel (Thermo Fisher Scientific), blotted onto nitrocellulose membrane and analyzed for EP300 and vinculin (loading control ...
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bioRxiv - Microbiology 2020Quote: ... and 3 μl/ml near-infrared live/dead fixable viability dye (Thermo Fisher, L94375) for 30 minutes on wet ice ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA digestion was done by different restriction enzymes (Table 3, Thermo Fisher Scientific, Germany). 5 units of restriction enzymes were added to each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... after which supernatants were incubated with 3 μl anti-V5 (Invitrogen R-960-25) or anti-HA (Invitrogen 26183 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... sonicated (3 × 45 s in a Sonic Dismembrator apparatus, Thermo Fisher Scientific, Waltham, MA) and centrifuged (100,000g ...
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng of plasmid was added to 3 µl of P3000 reagent (Life Technologies) in 75 µl of Opti-MEM prior to mixing with 2 µl of Lipofectamine 3000 (Life Technologies) ...
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bioRxiv - Genomics 2021Quote: RNA samples were extracted from mESCs (n = 3 biological replicates) using Trizol reagent (Invitrogen) and subjected to DNase digestion with Turbo DNase (Ambion AM2238) ...
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bioRxiv - Genetics 2020Quote: ... HRM assays using MeltDoctor reagents on a Quantstudio 3 (Applied Biosystems, Foster City, CA) were used to measure 5mC levels on the promoter and exon 2 of Sult1a1 and Ugt1a6 respectively (Supplementary Table S2) ...
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bioRxiv - Genetics 2021Quote: ... all measurements of RNA and DNA were performed on a Qubit 3 (ThermoFisher Scientific) using the appropriate assay kit ...
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bioRxiv - Evolutionary Biology 2020Quote: cDNA for 3’RACE was prepared using RLM-RACE kit (Ambion, Cat. No. AM1700) with 1µg MCF-7 total RNA as per the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged every 2-3 days by washing with HBSS (Gibco, cat#14170), trypsinizing using 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MMT) was obtained from Thermo Fisher. Crystal violet was purchased from VWR ...
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bioRxiv - Neuroscience 2020Quote: ... A biotin-labeled cRNA probe was generated using GeneChip 3’IVT Express Kit (Affymetrix). The probe was hybridized with a custom-made microarray (Marmo2a520631F ...
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bioRxiv - Biochemistry 2020Quote: ... Next 3-fold excess of Alexa Fluor 647 C2 Maleimide (Thermo Fisher Scientific #A20347) was mixed with the solution and the mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... qPCR analysis was performed on a Quantstudio 3 Real-Time PCR System (ThermoFisher Scientific) with the QuantStudio Design and Analysis Software (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... The epithelium medium was composed of a 3:1 mixture of DMEM (Hyclone, ThermoFisher) combined with Ham’s F12 medium (Gibco ...
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bioRxiv - Bioengineering 2020Quote: Nbs were diluted in a 2- or 3-fold series in Opti-MEM (Thermofisher). Each Nb dilution (110 μl ...
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bioRxiv - Bioengineering 2020Quote: 3 ug of Streptavidin-coated magnetic beads (Dynabeads MyOne Streptavidin T1, Thermo Fisher Scientific) was used for 1 pmole of a query strand ...
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bioRxiv - Developmental Biology 2021Quote: ... and used for relative quantitative PCR (QuantStudio 3 real Time PCR system; ThermoFisher scientific) using Power SYBR™ Green RNA-to-CT 1-Step Kit according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... Texas red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE) was purchased from Invitrogen. Alexa Fluor 555 maleimide dye and Alexa Fluor 488 maleimide dye were purchased from Life Technology ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative RT-PCR was run in QuantStudio 3 (Applied biosystems, Thermo Fisher Scientific, USA) using TaqMan Fast Virus 1-Step Master Mix (PN4453800 ...
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bioRxiv - Cell Biology 2021Quote: ... and run on an Applied Biosystems QuantStudio 3 real-time PCR machine (Thermo Fisher). Relative transcript levels were calculated using the ∆∆CT method and normalized to the ACTB gene ...
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bioRxiv - Physiology 2021Quote: ... cells were fed every 2-3 days with DMEM + 10% FBS (Thermo fisher Scientific) until differentiation was complete at day 10.
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with 3 mM dimethyl 3,3′-dithiobispropioimidate (DTBP; Thermo Fisher Scientific, #20665) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... HCT8 cells were grown on a 6-well slide (3×105/well, Thermo Scientific) overnight ...
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bioRxiv - Microbiology 2020Quote: ... the concentration determined with a Spectronic BioMate*3 UV spectrophotometer (Thermo Scientific, Waltham, MA) and 500 ng used as the template for cDNA synthesis ...
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bioRxiv - Microbiology 2020Quote: ... and DNA concentrations were determined using a Qubit 3 fluorometer (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... Axotomy was performed using 3 different methods: 1) Trypsin-EDTA 0.25% (Thermo Fisher Scientific) treatment for 15 min (Figure 4C ...
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bioRxiv - Molecular Biology 2020Quote: ... and Applied Biosystems QuantStudio 3 Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). Primers and probes used for SIV gag RNA quantification were as follows ...
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bioRxiv - Physiology 2021Quote: ... Amplification and detection of fluorescence were carried out on the QuantStudio 3 (Applied Biosystems) using the genotyping assay mode ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins in solution were cross-linked by 3 mM DSP (Thermo Fisher Scientific Pierce) and incubated during 2 h ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was precipitated by incubating in 3 μl (45 μg) glycoblue coprecipitant (Invitrogen), 18 μl 3M sodium acetate and 700 μl ethanol between 1 h and overnight at −80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was analyzed using the Quant Studio 3 Real-Time PCR system (Applied Biosystems) using Power SYBR® Green RNA-to-CT™ 1-Step Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells (3×105) were seeded in a 6 well plate (Nunc,Thermo,USA) with DMEM media (Invitrogen,USA ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times with PBS and mounted with Prolong Gold (Molecular Probes) containing 1 μg/ml DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... 1-3 µg were reverse transcribed with a MuLV reverse transcriptase (Applied Biosystems, UK) using random primers (Bioline ...