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Citations for Thermo Fisher :
9851 - 9900 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with 1 µg total DNA and 3 µL Lipofectamine 2000 (Invitrogen). Media was changed 4 h post-transfection to standard maintenance media and cells were harvested for microscopy or western blot 24 h post-transfection.
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bioRxiv - Bioengineering 2022Quote: ... Samples were then run on an Applied Biosystems QuantStudio 3 RT-PCR Machine (ThermoFisher).
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bioRxiv - Bioengineering 2022Quote: ... and mixed immediately with 3 molar excess of maleimide Alexa Fluor546 (#A10258, Thermo Scientific), followed by incubation at room temperature for another 2h ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR reactions were run on the QuantStudio 3 Real-Time PCR System (ThermoFisher). Normalization to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cell Biology 2022Quote: ... organoids were pre-treated with calcein green AM (3 μM; Invitrogen, Waltham, MA, USA) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... transfected cells were selected with 3 μg/ml puromycin (#A11138-03, Thermo Fisher Scientific) 48 h post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... cells were grown overnight in synthetic dropout media (Thermo Fisher cat # DF0919-15-3) at 30°C and imaged in log-phase (OD600 of ∼0.5 ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... cerebella were washed 3 × 1 h in 50% OCT (Fisher Scientific, 23-730-571) before embedding in 100% OCT ...
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bioRxiv - Developmental Biology 2022Quote: ... zLL 5’- and 3’- RACE PCRs were performed using the GeneRacer core kit (Invitrogen). Total RNA (2 µg ...
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bioRxiv - Physiology 2022Quote: ... on QuantStudio 3 Real-Time PCR system (Applied Biosystems, A28136, Foster City, CA, USA) to determine relative mRNA levels ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were run on a 3-8% Tris-acetate gel (Thermo Fisher Scientific, EA0375PK2) at 100V for 2 hours and wet transferred (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and 3) Poly(A) selection using Dynabeads™ Oligo(dT)25 beads (Invitrogen, #61005) to separate RNA into two populations ...
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bioRxiv - Microbiology 2022Quote: ... Amplification and detection of PCR products were performed using a QuantStudio 3 (Applied Biosystems).
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl RNase A (20-40 mg/ml) (Thermo Fisher Scientific, Waltham, MA, USA) and 15 μl proteinase K (10 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 2-3 sections were mounted on each Superfrost Plus slide (Thermo Fisher Scientific). All sections were air-dried at −20 ° C for 1 hour and afterwards stored at −80 ° C.
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bioRxiv - Physiology 2022Quote: ... and an (3) in-well control (Taqman probe Mm00437762_m1 B2M VIC PL, Thermofisher, 4448485). Each sample was loaded in triplicate ...
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bioRxiv - Neuroscience 2022Quote: ... Day 3: Media was changed into B27 media (1x B27 Supplement (Gibco, #17504-044), 1x Glutamax Supplement (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... Sodium chloride (NaCl S-271-3) and Triton X-100 (BP151) from Fisher Scientific. NP40 (198596 ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-claudin 3 polyclonal antibody (1:100 dilution, PA5-16867, Thermo Fisher Scientific); mouse anti-chondroitin sulfate antibody (1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... CCDC66 was depleted using an siRNA with the sequence 5′-CAGTGTAATCAGTTCACAAtt-3′ (Thermo Scientific). Silencer Select Negative Control No ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Slices were finally incubated for 3-6 minutes with DAPI (1:10 000, Invitrogen) diluted in PBS and mounted on Polysine® Slides stored at 4°C until imaging.
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bioRxiv - Cell Biology 2022Quote: ... while 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine-5,5′-disulfonic acid (DilC18) was purchased from Invitrogen. All stock solutions were prepared in chloroform/methanol (2:1 ...
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bioRxiv - Physiology 2024Quote: ... and oligo primers for target genes and carried out on QuantStudio 3 (Applied Biosystems). Transcripts of SOCE and Ca2+ signaling related proteins were quantified in quadriceps muscles from 6 months old WT and C3KO mice ...
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bioRxiv - Neuroscience 2023Quote: FOs from 2 or 3 independent differentiations were fixed using 4% paraformaldehyde (Thermo Scientific) in PBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen, NuPAGE). After transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Biochemistry 2024Quote: ... followed by 3-min permeabilization using 0.15% Surfact-Amps X-100 (28314, Thermo Fisher) in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 min and 1:500 of Hoechst 33342 (20 mM, Thermo Fisher Scientific) for 10 min ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were passaged every 2-3 days using 0.05% Trypsin/EDTA (Gibco™, 25300062). Cell density was 5x 104 per well for the experiments in the μ-Slide (ibidi ...
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bioRxiv - Pathology 2024Quote: Ventricular myocytes were loaded with 3 µM Fura2-AM (Invitrogen, Molecular Probes, Carlsbad CA) in a modified Tyrode’s solution containing (in mM ...
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bioRxiv - Pathology 2024Quote: Ventricular myocytes were loaded with 3 µM Fura2-AM (Invitrogen, Molecular Probes, Carlsbad CA) in a modified Tyrode’s solution containing (in mM ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was replaced after 3-4 hr with neurobasal medium (Gibco, Cat# 21103049) supplemented with 2% v/v B27 (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, Thermo Scientific) assay was performed on cells seeded and treated in 96 plates for 72 hours with the free doxorubicin or doxo-EVs ...
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bioRxiv - Cell Biology 2024Quote: IMCD-3 cells expressing mScarlet tubulin were then transfected in OptiMEM media (Thermo Scientific) using Lipofectamine 2000 (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... The RNA concentration was estimated using a Qubit 3 Fluorometer (Thermo Fisher Scientific, USA). RNA quality was assessed using TapeStation (Agilent Technologies 4200 ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed 3 times with DMEM and fixed with 4% paraformaldehyde (Thermo Fisher) at room temperature (RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were run on the QuantStudio™ 3 Real-Time PCR System (Applied Biosystems) with the same parameters listed in the transgene copy-number determination section above ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were run on the QuantStudio™ 3 Real-Time PCR System (Applied Biosystems) using the Fast Run Mode with the following reaction conditions ...
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bioRxiv - Microbiology 2024Quote: ... 3% active NaClO with 20 µL of Tween 20 (Thermo Fisher, Waltham MA, US) for 120 s ...
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bioRxiv - Immunology 2024Quote: ... Cells were grown to 3×106 cells/mL and transfected using Expifectamine (Life Technologies) with 1 μg of plasmid per mL of culture media ...
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bioRxiv - Molecular Biology 2024Quote: Cells were stained using 3 mM stock solution of Nile Red (Thermo Fisher, Invitrogen) (used for staining membranes ...
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bioRxiv - Molecular Biology 2024Quote: Cells were stained using 3 mM stock solution of Nile Red (Thermo Fisher, Invitrogen) (used for staining membranes ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated in a blocking solution containing 3% bovine serum albumin (Gibco), 0.1% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... Slices were then incubated in PBS supplemented with 3% normal goat serum (NGS, Invitrogen) and 0.2% Triton overnight at 4°C and then in primary antibodies diluted in the same buffer at 4°C till the next day ...
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bioRxiv - Physiology 2023Quote: ... Reverse Transcriptase Quantitative PCR was done using the QuantStudio™ 3 System (Applied Biosystems) with Power SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... the medium was switched to SFRM (DMEM/F12 [7:3] supplemented with B27 (Invitrogen), 2 mM L-glutamine).
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bioRxiv - Biochemistry 2023Quote: ... Native samples were separated using NativePAGE 3-12% Bis-Tris gels (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... columns were washed with 3 column volumes (CV) of 1:1 RNase ZAP (Ambion) followed by 3 CV nuclease-free water (DEPC-treated MilliQ water) ...
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bioRxiv - Neuroscience 2023Quote: ... samples were loaded and run on NuPAGE 3-8% Tris-Acetate Gel (Invitrogen, EA0378BOX) at 120V for 15 minutes then 180V for 40 minutes ...