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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... A 2 μl aliquot of a 1:10 dilution of the ERCC RNA Spike-in Mix (Thermo Fisher Scientific) were added to each sample ...
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bioRxiv - Developmental Biology 2021Quote: Two BCAM-targeting siRNAs (siBCAM #1, siBCAM #2; Dharmacon) were transfected into CT29 hTSCs using Lipofectamine RNAiMAX (Thermo Fisher) at a concentration of 100 nM ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 555-conjugated F(ab’)2 fragment donkey anti-rabbit (1:500; A31572, Invitrogen, Thermo Fisher Scientific, Waltham, MA), AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 555-conjugated F(ab’)2 fragment donkey anti-rabbit (1:500; A31572, Invitrogen, Thermo Fisher Scientific, Waltham, MA), AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy3-labeled species-specific secondary antibodies for 2 h at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch). To avoid cross talk between multiple fluorophores ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Chromosomes were counterstained for 20 minutes with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; ThermoFisher Scientific, D3571) in 2x SSC ...
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bioRxiv - Neuroscience 2020Quote: ... before 2-h incubation at room temperature with a secondary antibody Alexa 555 made in rabbit (Invitrogen, 1:500). After several washes ...
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bioRxiv - Systems Biology 2021Quote: We isolated pre-adipocyte cells from subcutaneous adipose tissue by dissecting tissue into small (1-2 mm) pieces with scissors and performing a 0.1% (w/v) collagenase II (Gibco) digestion in DMEM at 37oC for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Each 20-μlL PCR mixture contained 2 μL of 10X Accuprime PCR buffer II (Life Technologies, CA, 1 USA), 0.15 μL of Accuprime high-fidelity polymerase (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then run on a Native PAGE gel and visualized with Alcian Blue (1:20 dilution of 1.25% stock solution in 2% acetic acid) followed by Silver Staining (ThermoFisher) following manufacturer protocols and imaged on the Bio-Rad Chemidoc MP Imaging System.
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bioRxiv - Microbiology 2021Quote: ... 2.5 mM probenecid were added to the cultures which were diluted 1:2 in DMEM with GlutaMAX (Gibco, Germany). 50 µl of the bacterial suspension were subsequently added to the screening plates which contained 1 µl of compound per well (concentrations are given in Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 and 3 dpf embryos were injected with 1–2 nL dextran tetramethyl rhodamine or dextran fluorescein (molecular weight 2,000 kDa, Invitrogen) at 10 mg/mL and imaged immediately ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 cells or Py8119 cells suspended at 1-2 million/150 µl of 33% (v/v) matrigel (Fisher Scientific) were transplanted subcutaneously to the right flank of 6-8 week-old BALB/c or C57BL/6J female mice ...
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bioRxiv - Cell Biology 2022Quote: ... iPSCs were transfected with PB-TO-hNGN2 vector (gift from M. Ward, NIH, Maryland) in a 1:2 ratio (transposase:vector) using Lipofectamine Stem (ThermoFisher); after 72 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... and then washed with 10% formamide in 2× SSC solution with Hoechst 33342 (1:1000, Thermo Fisher Scientific, H3570) and incubated at 37°C for 30 min in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µl of 2 µM Uni9 primer and 1µl of 10 mM dNTP (Thermo Scientific™, Cat no. # R0181) were mixed and warmed at 65 ºC for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... bacteria were washed by centrifugation three times (11000 rcf for 2 minutes) and resuspended to OD 1.0 (~1 × 109 bacteria/ml) in RPMI (Gibco) + 0.05% human serum albumin (HSA ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were electroporated with 2 pulses at 65 mV for 20 ms in a 1 mm cuvette (ThermoFisher, #15437270). After electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... The coverslips were 1-or 2-days post-transfection mounted in an AttoFluor cell chamber (A7816, Thermo Fisher Scientific) and microscopy medium (137 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: A solution of 2 μM of Calcein-AM and 8 μM of ethidium homodimer-1 (both from L3224, ThermoFisher) in DPBS (14190-144 ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue powder was transferred to 2 mL microcentrifuge tube and 1 mL TRIzol Reagent (ThermoFisher, Cat no 15596018) was added ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were split 1-2 times per week after dissociation with EDTA in DPBS (final concentration 0.5mM) (Gibco, 12605010). Media was supplemented with Rho-associated protein kinase (ROCK ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Immunology 2022Quote: ... 1.5×106 CD45.1 cells were incubated with 2×106 Balb/c splenocytes in the presence of 1 mg/mL GolgiStop (Invitrogen) for 4-5 h at 37 C ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized using a 2:1 ratio of random primers to Oligo(dT) with SuperScript IV RT (Invitrogen). qPCR was performed with PerfeCTa SYBR Green FastMix for iQ (Quanta Biosciences ...
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bioRxiv - Genetics 2021Quote: ... 20 μl reaction mix with 2 μl cDNA (~1-10 ng) were monitored on StepOnePlus Thermal Cycler (Applied Biosystems) in “fast mode” ...
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bioRxiv - Genomics 2021Quote: ... The extracted RNA (1-2 μg) was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies). Quantitative PCR was carried out in the BioRad CFX96 Real-Time System using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... VH and VL plasmids were mixed at 1:2 ratio and were transfected into Expi293F cells (Thermo Fisher Scientific), which were cultured at 37 °C and 8% CO2 for 5 days ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of linearised plasmid was incubated for 2 h at 37°C with 1U T7 RNA polymerase (ThermoFisher), 5 mM DTT ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... RNA was treated 1 h at 37 °C with 2 units of Turbo DNAse (ThermoFisher Scientific, Waltham, MA, USA), purified using NucleoSpin RNA Clean-up (Macherey-Nagel ...
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bioRxiv - Microbiology 2021Quote: ... followed by 2 h staining with secondary donkey anti-mouse Alexa Fluor 546 antibody (Invitrogen, A10036, 1:500 dilution) and 1μg/ml DAPI.
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bioRxiv - Immunology 2021Quote: ... in R10 media (RPMI 1640 with 10% FCS, 1% penicillin/ streptomycin, and 2 mM L-glutamine, Thermo Fisher Scientific) at 37°C in 5% CO2 to differentiate quiescent MBCs into mASCs ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspendend in 1 mL M9 before 5 µL of DiI stock solution (2 mg/mL DiI (Molecular Probes, #D282) in dimethyl formamide ...
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bioRxiv - Immunology 2021Quote: ... The sections were then incubated for 2 hours at room temperature using: rabbit polyclonal antibody-1 for myeloperoxidase (Invitrogen) to examine neutrophils ...
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bioRxiv - Cancer Biology 2022Quote: ... were incubated with 100 μl of 2 mg ml-1 p-NPP (p-nitrophenyl phosphate) substrate (#34045; Thermo Fisher) diluted in AP-buffer ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated for 2 h with secondary antibodies conjugated to Alexa-488 (1:2000; Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Human β-arrestin 2 (residues 1-393, with I386A, V387A, and F388A mutations) was cloned into pBiT2.1 vector (Invitrogen) with the SmBiT at its N-terminus ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% (v/v) sodium deoxycholate) supplemented with 1% protease inhibitor cocktail and 2% RiboLock RNase Inhibitor (#EO0381, Thermo Scientific). The RNA was sheared ...
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bioRxiv - Molecular Biology 2023Quote: 1×105 HeLaK cells expressing GFP or GFP-ZNF512B or treated with Doxorubicin (100 nM, 2 days, Fisher Scientific) were seeded on coverslips in 24-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were stained with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, 1:1000, Invitrogen, Thermo Fisher Scientific, MA) for 10 mins ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNAs were synthesized from total RNA (1 μg) for each sample using SuperScript 2 reverse transcriptase (18064014, Thermo Fisher), diluted ten times with water ...
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bioRxiv - Neuroscience 2023Quote: ... Cell nuclei were stained with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, 1:1000, Invitrogen, Thermo Fisher Scientific, MA) for 10 mins ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with serum free RPMI 1640 medium and incubated with 1 μM Rhod-2-AM (Invitrogen, R1244) together with antibodies for CD4 (GK1.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The PAA gel surface was then incubated with a solution of 2 mg ml−1 Sulpho-SANPAH (4822589, ThermoFisher) under ultraviolet light for 5 min (wavelength of 365 nm at 5 cm distance) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM 2-mercaptoethanol and 10 mM imidazole) and then loaded onto a HisTrap HP column (ThermoFisher, Waltham, USA). The eluted fractions containing FHS-OGOX1 were pooled and dialyzed in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... 1:2000 LipidTOX was added along with 0.25 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes) for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was replaced daily from 8 hpf and supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) to inhibit melanogenesis ...