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Citations for Thermo Fisher :
9501 - 9550 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The plasmid SV-Psi--Env--MLV and L-LUC-SN were co-transfected with or without an envelope glycoprotein plasmid (pHCMV-VSV-G/pSARS-CoV-1/pSARS-CoV-2/pHIV-1 LAI gp160) into HEK293FT cells using Lipofectamine™ 3000 (ThermoFisher, US). Cell supernatants containing viruses were collected after 2 days of transfection ...
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bioRxiv - Genomics 2020Quote: ... or No Call (numerical representations 0, 1, 2, −1, respectively) using the BRLMM-P algorithm for 493,290 SNPs [25] (Affymetrix Power Tools (APT) Release 1.16.0) ...
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bioRxiv - Genomics 2020Quote: ... around 100 mg of liquid nitrogen frozen samples were ground with metal beads (2.5 mm diameter) in a Retschmill apparatus (24 hertz for 2×1 min) before re-suspending in 1 mL Trizol reagent (Thermo Fisher Scientific) and incubating at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Triton X-100 in 200 μL of PBS for 5 min and subsequently incubated in 100 μL of click reaction buffer (1× Click-iT cell reaction buffer [Thermo Fisher Scientific], 2 mM CuSO4, 1× Click-iT cell buffer additive [Thermo Fisher Scientific] ...
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bioRxiv - Biochemistry 2021Quote: ... equal numbers of THP-1 monocytes (2*105 ml−1) were seeded in RPMI 1640 without Lysine and Arginine (Thermo Fisher Scientific), supplemented either with natural amino acids (L-Lysine ...
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bioRxiv - Cancer Biology 2021Quote: Single live cell suspensions were washed with 1% FCS in PBS and resuspended in flow cytometry buffer (2% FCS and 1 mM ethylenediaminetetraacetic acid (EDTA; Thermo Fisher #AM9260G) in PBS).
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bioRxiv - Developmental Biology 2021Quote: ... The colonies were lifted with a 1:1 Dispase/Collagenase enzyme mix (1mg/ml; Gibco; 17105-041 and 2 mg/ml; Gibco; 17104-019). and resuspended in 10 ml of Phase 1 medium (1:1 IMDM (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
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bioRxiv - Neuroscience 2022Quote: ... iAstrocyte differentiation was initiated by switching the medium to astrocyte medium (DMEM/F12+GlutaMAX, 1% N-2 supplement, 1% fetal bovine serum, all from Thermo Fisher Scientific) as previously described [81] and continued for at least 60 days ...
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bioRxiv - Microbiology 2022Quote: ... Human neuronal BE(2)-M17 cells (ATCC® CRL-2267) were maintained in a 1:1 ratio of F12 Hams and OptiMEM (GIBCO, USA) media supplemented with 10% horse serum (GIBCO ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with TBS for 10 min and then then nuclei were counterstained by incubation with diamidino-2-phenylindole dye (DAPI) (1:30,000 in PBS, from a 1 mg/ml stock solution, Thermo Fisher Scientific #62248) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... Accutase was removed and the cells were then resuspended in DMEM with 2% B-27-supplemented 1% FBS and 1% PSF (Thermo Fisher Scientific) and plated on PDL/Laminin-2020-coated culture plates.
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bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
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bioRxiv - Cell Biology 2023Quote: ... and the nucleus was stained with diamidino-2-phenylindole dye (DAPI) (1:30,000 in PBS, from 1 mg/ml stock solution (Thermo Fisher Scientific #62248)) ...
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bioRxiv - Microbiology 2023Quote: ... cells with either MX2-T2A-TurboID or MX2-MUTT2A-TurboID lentivirus and selecting with 2 µg ml−1 puromycin (#ant-pr-1, Thermo Fisher Scientific) for 7 days ...
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bioRxiv - Microbiology 2023Quote: Pseudotyped viruses were titrated by serially diluting filtered pseudotypes with fresh DMEM starting from a 1:2 to a final 1:512 dilution using white 96-well flat bottom plates (ThermoFisher, Woolwich, UK). Then appropriate target cells were added (Table 1 ...
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bioRxiv - Genetics 2023Quote: ... The solution was then exchanged with primary antibody diluted in a staining solution (1% DMSO, 0.5% Triton X-100, and 2% NDS in PBS): Sox2 (1:200; Invitrogen, 14-9811-82) or AF647-conjugated β-catenin (1:200 ...
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bioRxiv - Zoology 2023Quote: ... stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse (Molecular Probes, 1:1000), in PBST at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
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bioRxiv - Cell Biology 2023Quote: ... and fibroblasts were cultured for 2 days in DMEM/F12 (1/1) supplemented with 10 % fetal bovine serum (Fetal Clone II; Hyclone, Thermo Scientific, France) and 1% Penicillin/Streptomycin (Sigma) ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 Nucleocapsid antibody (produced in mouse) as a primary antibody was procured from (MA5-35943, Invitrogen, 1:1000-1:10,000), Rabbit phospho-p38 MAPK and total p38 MAPK Antibody were procured from (9211S and 9212S ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice with PBS (1X) and incubated for 2 hours at 37°C with secondary detection antibodies (1:500) and phalloidin (1: 3000) (Invitrogen, Waltham, MA) for cytoskeleton staining ...
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bioRxiv - Microbiology 2023Quote: ... mosquito bodies and legs were collected and placed in homogenizer tubes with a homogenizer bead and 500μl mosquito homogenization media (DMEM containing 2% FBS, 1% PenStrep, and 1% Fungizone (Gibco, Grand Island, NY)) ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were labeled with 1 μg/mL mAb 13-2 (rOmpB) (53) and 1 μg/mL rabbit polyclonal αTGN46 (Invitrogen, PA5-23068) in PBS overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg) was injected onto a Dionex UltiMate 3000 RSLCnano LC system (Thermo Fisher Scientific) with an additional RSLCnano pump run in online two-dimensional active modulation mode coupled to a Thermo Fusion Orbitrap mass spectrometer.
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bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated at 37°C for 1 hr under 200 rpm with 2 mg/mL collagenase III (StemCell, #07422) in a complete medium consisting of Hyclone DMEM/F/12 1:1 (Thermo Scientific, #SH30023.01) supplemented with 15mM HEPES ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ml of collagenase mix digestion solution (collagenase II + IV mixed at 1:1 ratio at 2 mg/ml -Thermo Fisher Scientific) was added to the tissue and incubated horizontally for 20 min at 37°C in a shaking incubator ...
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bioRxiv - Cell Biology 2024Quote: ... Then CB were diluted 1:1 (v/v) into a dilution buffer (1mM EDTA in PBS supplemented by 2% FBS (Thermo Fisher, #10099141)) and undelayed with density gradient medium LymphoPrep (Stemcell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... All cultures received N2 medium from d0 to 11 (50% MACS Neuromedium, 50% DMEM/F12 containing 1% N-2 supplement [Thermo Fisher Scientific, #17502048] 1% GlutaMAX [Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Sort-purified cells (∼1-5×106) were cross-linked for 10 min in 1 ml of 2% methanol-free formaldehyde (Thermo Fisher Scientific) in PBS + 3% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... either untreated or treated with the cytokines listed above were diluted either 1:2 or 1:100 and ran using a Luminex Flexmap Assay (ThermoFisher, Lot# L152023) to probe for IL-6 ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies used for immunostaining were anti-tyrosinated α-tubulin (YL1/2) (1/25 ECACC) and anti-rat Alexa Fluor 555 secondary antibody (1/400, Life Technologies). Phalloidin conjugated with and Alexa Fluor 647 A22287(1/200 ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (5,000 cell/well) ...
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bioRxiv - Microbiology 2020Quote: ... The qRT-PCR assays were performed to quantify the amount of SARS-CoV-2 RNA with QuantStudio 3 instrument (Thermo Fisher Scientific, Waltham, MA, USA) using primers and probes described previously8,9 (N2 set or E set).
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (3,000 cell/well in 200 μL of complete medium and cultured under standard conditions for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were seeded onto polyethylenimine-coated 3-cm dishes (2 × 105 cells/dish) and cultured in neurobasal medium (Gibco; Thermo Fisher, Waltham, MA, USA) supplemented with B-27 and 2 mM Glutamax (Gibco ...
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bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
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bioRxiv - Immunology 2023Quote: ... Each sample was injected onto a PepMap C18 trap column (75 μM X 2 cm, 3 μM, 100 Å, Thermo Fisher Scientific, San Jose, CA) at 5 μL/min for 6 min using 0.05% trifluoroacetic acid (TFA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with each sample being prepared from 5 individuals (3 karyotypes x 2 temperatures x 9 isochromosomal lines = 54 samples) using the MagMAX™-96 Total RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) on a MagMAX™ Express Magnetic Particle Processor (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA). Viral supernatants were collected 48 hours post-transfection and filtered through a 0.45 μm Nalgene syringe filter SFCA (Whatman ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was reverse crosslinked by incubation at 68°C for 2 h and purified by phenol:chloroform:isoamyl alcohol 25:24:1 (Life Technologies) extraction and ethanol precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... They were then maintained in RPE medium (70% DMEM, 30% Ham’s F12 nutrient mix, 2% B27-serum-free supplement, 1% antibiotic-antimycotic solution (Invitrogen)) for 2-3 months to form mature RPE monolayers ...
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bioRxiv - Cell Biology 2020Quote: ... the suspension was diluted 1:2 with fresh medium and passed through a 40 μm cell strainer (Fisher Scientific) to obtain single cell suspension ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Mouse T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (ARGi ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (1500 nM) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Nickase-1/2 constructs were individually transfected into CH12 via electroporation with Neon Transfection System (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... diluted (1:10,000) in pre-incubation (PI) buffer (PBS, 0.1% Tween 20, 2% sheep serum, 2mg/ml BSA (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed with PBS followed by staining using streptavidin 488 (1:300, Jackson) diluted in PBS containing 0.4% Triton-X and 2% normal horse serum (NHS; Gibco) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... secondary Ab was incubated for 2 hours at room temperature (Alexa568-conjugated anti-mouse Ab, 1:400, Life Technologies). Brains were then mounted in Vectashield (Vector Labs ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl reaction mix with 2 μl cDNA (∼1-10 ng) were monitored on StepOnePlus Thermal Cycler (Applied Biosystems) in “fast mode” ...