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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... RNA-protein complexes and the unbound RNA probe were detected using the Chemiluminescent Nucleic Acid Detection Module (Thermo Scientific), following manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... diluted in 98μl 0.9% NaCl and stained with 0.25μl SYTO-16 Green Fluorescent Nucleic Acid Stain (ThermoFisher Scientific, catalog # S7578) within 4 hours of collection ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in sorting buffer (0.2% BSA in PBS) containing 5 nM SYTOX Green Nucleic Acid Stain (catalog number S7020; ThermoFisher) to distinguish between live and dead cells ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acids were extracted using the PureLink viral RNA/DNA extraction kit according to the manufacturer’s instructions (Invitrogen, USA), and eluted in nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid was purified from the submitted swab sample using the MagMAX-96 Viral RNA isolation kit from ThermoFisher. Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections from DREADD experiments were mounted with DAPI-containing mounting medium (nucleic-acid counter-stain; Fluoromount-G, Invitrogen); the presence and location of DREADD expressing cells were verified with laser scanning confocal microscopy (Fig ...
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bioRxiv - Physiology 2022Quote: ... The nucleic acids present in the pituitary gland cells were visualised with TOTO-3 (1:2000, Thermo Fisher Scientific). Sections were incubated with TOTO-3 for 15 min on an agitated surface ...
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bioRxiv - Microbiology 2019Quote: ... day 1 (24 hours) and day 3 (72 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were resuspended in 15 µl of TE buffer and quantified in a NanoDrop Nucleic Acid Quantification (Thermo Fisher). 200 ng of genomic DNA were loaded in an ethidium bromide-stained 1% agarose gel ...
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bioRxiv - Synthetic Biology 2021Quote: ... Nucleic acids concentration was measured by absorbance at 260 nm using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, USA). All selections were performed using SELEX buffer (100 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... All nucleic acids isolated in this study were quantified using a Nanodrop 1000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA). A QIAprep Spin Miniprep kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... cell medium was replaced by fresh medium supplemented with 5 µM Hoechst 33342 nucleic acid stain (Thermo Fisher Scientific) as a background stain for high-content imaging analysis ...
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bioRxiv - Molecular Biology 2021Quote: We assayed extracted nucleic acids from all samples using a custom TaqMan Array Card (TAC) (ThermoFisher Scientific, Waltham, MA) that tested for 20 enteric pathogens in duplicate wells following Liu et al ...
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bioRxiv - Neuroscience 2022Quote: ... total RNA was purified from selected material using a RecoverAll™ Total Nucleic Acid Isolation kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... to label filamentous actin followed by DAPI nucleic acid stain (DAPI dihydrochloride, 300 nM in PBS, Invitrogen, United Kingdom). Filter membranes were washed then mounted with ProLong Glass antifade mountant (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... then stained with 8.08 μL of 1X Invitrogen SYBR Green nucleic acid stain (Thermo Fisher Scientific, Carlsbad, CA, USA) and incubated for 10 min at 37 °C before measurement.
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bioRxiv - Microbiology 2023Quote: ... Nucleic acids were extracted twice from lysates using Phenol/Chloroform/Isoamyl Alcohol 25:24:1 (ThermoFisher Scientific Cat#10308293) treatment followed by centrifugation at 8,000 g for 5 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Parasitemia was monitored by flow cytometry using the nucleic acid stain SYTO 11 (0.016 μM) (Life Technologies no. S7573) in a BD LSRFortessa machine as previously described (Portugaliza et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... biotin-labeled oligonucleotide probes were used together with chemiluminescent nucleic acid detection kits according to manufacturer’s instructions (Thermo Scientific). The 18S RNA from the agarose gel run was used as loading control ...
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bioRxiv - Developmental Biology 2023Quote: hiPSCs in alginate hydrogels were incubated with SYTOX Green Nucleic Acid Stain (1 μM; Invitrogen, Thermo Fisher Scientific S7020) for 1 hr at 37°C and imaged on a Leica SP8 laser scanning confocal microscope with a HC FLUOTAR L 25×/0.95 NA water immersion objective.
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bioRxiv - Developmental Biology 2023Quote: hiPSCs in alginate hydrogels were incubated with SYTOX Green Nucleic Acid Stain (1 μM; Invitrogen, Thermo Fisher Scientific S7020) for 1 hr at 37°C and imaged on a Leica SP8 laser scanning confocal microscope with a HC FLUOTAR L 25×/0.95 NA water immersion objective.
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bioRxiv - Microbiology 2023Quote: ... PAO1-Phi2 and PAO1-Phi4 (at 1×107 CFU ml-1) were inoculated with 10µM Sytox green nucleic acid stain (Thermofisher). These were left at room temperature for 15min to enable absorption of the stain and then grown at 37°C in a plate reader (Clariostar ...
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bioRxiv - Genomics 2024Quote: All polynucleotide probes were chemically labeled with the Alexa Fluor 594 using the Ulysis Nucleic Acid Labeling Kit (ThermoFisher) following the same modifications to the manufacturers’ protocol as in (Barrero-Canosa et al. ...
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bioRxiv - Plant Biology 2023Quote: ... the hybridization between probe and RNA was detected using a Chemiluminescent Nucleic Acid Detection Module kit (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... kidney slices were washed in PBS before incubating in 5:1 SYTO 9 and PI nucleic acid stain (7.5 µL for 15 minutes with shaking (LIVE/DEAD BacLight Viability Kit, L7012, Invitrogen). Samples were blotted dry with Kimwipes and placed on modified histology cassettes for imaging.
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were fractionated by SDS-PAGE gel electrophoresis on 4–12% (or 12%) Nupage Bis–Tris gels with MOPS running buffer (#161-0788, Invitrogen, USA) and transferred to cellulose membranes (#10600016 ...
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bioRxiv - Cell Biology 2022Quote: ... and the samples were subjected to SDS–polyacrylamide gel electrophoresis on NuPAGE 4–12% Bis-Tris gradient gels (Novex Life Technologies). The proteins were then transferred onto a nitrocellulose membrane (Millipore) ...
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bioRxiv - Cell Biology 2019Quote: ... prior to SDS-PAGE gel electrophoresis on 1.5 mm NuPAGE Novex 4-12 % Bis-Tris Protein Gels (Thermo Fisher Scientific, USA), using Mini Trans-Blot Cell system (BioRad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We selected libraries within the size range of 450-500 bp using a 2% E-gel on an E-Gel Electrophoresis System (Invitrogen, Canada), and re-amplified the size-selected libraries using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: Cultured cells were collected and lysed with RIPA buffer and a total of 20 μg protein was separated by SDS-polyacrylamide gel electrophoresis using 4-12% NuPAGE Bis-Tris gels (ThermoFisher Scientific), blotted to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... Deglycosylation on the standard proteins was analyzed by gel electrophoresis on 15% SDS-PAGE gels and the gels stained by Coomassie Brilliant Blue R-250 Dye (Cat. 20278, Thermo Scientific). The optimal conditions were applied to study limited deglycosylation on LLC-MK2 cellular glycoproteins upon induction of ER stress ...
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bioRxiv - Microbiology 2021Quote: Proteins extracted from cell lysates were resolved by SDS-polyacrylamide gel electrophoresis on 4-12% NuPAGE Bis-Tris Gel (Life Technologies) with MOPS running buffer and semi-dry transferred to a nitrocellulose membrane with Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR product visualization was performed using agarose gel electrophoresis with appropriate agarose concentration and SYBR® Safe DNA Gel Stain (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... After protease exposure the samples were analysed by reducing and denaturing polyacrylamide gel electrophoresis on 4-12% bis-tris gels (Life Technologies). Protein bands were developed with Simply Blue Safe Stain (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then separated using gel electrophoresis using 4-12% Bis-Tris Novex Midi 20 well gels (Invitrogen, Cat. No. #WG1402BOX) with 1X MES Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The mixtures were then analyzed by electrophoresis through a urea-15% polyacrylamide gel (Novex TBE-Urea gel 15%, Thermo Fisher Scientific). To acquire the fluorescent signals ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates of HAP1 and HeLa cells were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% gradient precast polyacrylamide gels (Life Technologies). The proteins separated by SDS-PAGE were transferred onto a nitrocellulose membrane using a Mini-Protean® Tetra Cell device ...
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bioRxiv - Neuroscience 2021Quote: ... The designated samples were then subjected to one-dimensional gel electrophoresis (1DE) employing precast NuPAGE 4-12% Bis-Tris 10-well mini protein gels (Invitrogen, Germany) with 2-[N-morpholino]ethanesulfonic acid (MOPS ...
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bioRxiv - Microbiology 2020Quote: Protein samples were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) using NUPAGE 10% Bis-Tris Gels (Life Technologies), run in NUPAGE MES Running Buffer (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using NuPAGE Bis-Tris gels (Thermo Fisher Scientic) and transferred on nitrocellulose membranes (iBlot 2® gel transfer Stacks Nitrocellulose system from Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... of SEC-purified small EVs and cell lysates were separated by SDS-polyacrylamide gel electrophoresis (PAGE; NuPAGE 4-12% Bis-Tris Protein Gel, ThermoFisher Scientific) and transferred to PVDF membranes (Millipore ...
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bioRxiv - Pathology 2022Quote: ... sites flanking the donor DNA sequence and the linearized donor DNA was purified by agarose gel electrophoresis with GeneJet Gel Extraction kit (Thermo Fisher). Linearized donor DNA and CRISPR/Cas9 complex were injected into C57BL/6 fertilized zygotes ...
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bioRxiv - Molecular Biology 2020Quote: ... ref.17-1318-02) before being subjected to SDS-PAGE gel electrophoresis (NuPage™ 4-12% Bis-Tris Gel Invitrogen, NP0322box) and semi-dry transfer to a nitrocellulose membrane (iBlot®2NC Mini Stacks Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... after denaturation by 70°C for 3 minutes and separated by gel electrophoresis using 10% TBE-Urea gels (Invitrogen, Cat# EC68755BOX) at 180V for 60 min in 1x TBE electrophoresis buffer (Bio-Rad ...
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bioRxiv - Pathology 2021Quote: ... The PCR amplicon with an attB sequence at the end was visualized using gel electrophoresis and purified using the GeneJet Gel Extraction Kit (Thermo Scientific). Fragments were cloned into the pDONOR vector via a BP Clonase reaction (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were analyzed by 0.8% or 2% agarose gel electrophoresis and visualized under UV after staining with SYBR safe DNA gel stain (Invitrogen, Carlsbad, California). PCR products were purified with the Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Immunology 2022Quote: ... and alkylated with 10mM iodoacetamide prior to sample fractionation by polyacrylamide gel electrophoresis with Novex NuPAGE Bis-Tris 4–12% gels (Life Technologies). Gels were stained with colloidal Coomassie (Sigma ...