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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... PCR product was analyzed by gel electrophoresis on a 0.8% agarose gel stained with SybrSafe and compared to a one kb Plus Ladder (Invitrogen). RT-PCR amplifications was performed for all the samplings harvested ...
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bioRxiv - Genomics 2020Quote: ... Products were visualized on a 2% E-Gel EX agarose gel run on a Power Snap Electrophoresis Device (ThermoFisher) to verify size ranges and quantified using a Qubit 2.0 Fluorometer and dsDNA HS Assay Kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2020Quote: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 3-8% Tris-Acetate gels (Invitrogen, Carlsbad, CA), as described previously [27] ...
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bioRxiv - Neuroscience 2022Quote: ... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using precast 4-12% polyacrylamide gradient gels (ThermoFisher, NW04122BOX), submerged at 4°C in Bolt™ MOPS SDS Running Buffer (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... PCR fragments were isolated from 1 % agarose gel after electrophoresis and purified by GeneJET Gel Extraction Kit (Thermo Fisher). The fragment generated was inserted into the PJET1.2/blunt cloning vector (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Equal quantities of cellular DNA from different conditions of the experiments were subjected to gel electrophoresis in 1.4% agarose gel with SYBR safe dye (Invitrogen). Agarose gel electrophoresis was done at 50V (5V/cm ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of protein (30-50 µg) were fractionated on 4-12% gradient SDS-polyacrylamide gel electrophoresis gel (Invitrogen) and transferred to PVDF membrane (Roche) ...
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bioRxiv - Plant Biology 2019Quote: ... loaded on leaf area basis and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation ...
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bioRxiv - Plant Biology 2021Quote: ... and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation, Carlsbad, CA). Proteins were transferred to a nitrocellulose membrane and probed against photosynthetic proteins with antibodies according to manufacturer’s protocols (Agrisera ...
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bioRxiv - Biochemistry 2021Quote: ... Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was performed on a NuPAGE Novex Bis-Tris gel following manufacturer’s instructions (Invitrogen). Protein/ACE2 or protein/mAb attached on magnetic beads or protein control in solution were mixed with 4X Sample Buffer and deionized water (1:3 v/v ...
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bioRxiv - Genetics 2021Quote: ... were taken to reducing sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE; Gels: ThermoFisher NuPAGE 4 to 12 %, Bis-Tris). The separated proteins were transferred to nitrocellulose membranes (0.2 µm ...
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bioRxiv - Microbiology 2020Quote: ... Products were separated by agarose gel electrophoresis and purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher K210012). Samples were then diluted to 2–4 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested products were analysed by electrophoresis on a 1.5% agarose gel stained with SYBR Safe DNA Gel Stain (InvitroGen). The experiment was run for 1 h at 145 V and the gel was visualized under UV light using a BioSpectrum Imaging System (Ultra-Violet Products Ltd.) ...
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bioRxiv - Microbiology 2022Quote: ... Product sizes were evaluated using gel electrophoresis (1.5% agarose) with SYBR Safe DNA Gel Stain (Fisher Scientific, Chicago, IL) and purified using a Qiagen PCR Purification Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR fragments were analyzed by gel electrophoresis with 1.8% agarose gels in TBE buffer containing 20000x SYBR Safe (S33102, Invitrogen). 5 μl of PCR products were loaded in each well ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound S protein/ACE2 or S protein/mAb complexes were analyzed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) on a NuPAGE Novex Bis-Tris gel following manufacturer’s instructions (Invitrogen). Protein solutions were mixed with 4× sample buffer and deionized water (1:3 v/v ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by SDS-PAGE gel electrophoresis on 1 mM NuPAGE 4 – 12% bis-tris gels (Thermo Fisher) using MOPS running buffer and transferred to nitrocellulose membranes using Tris-Glycine-methanol transfer buffer (30.3 g Tris Base ...
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bioRxiv - Genomics 2023Quote: ... The E-gel was then loaded onto the E-Gel Power Snap Electrophoresis Device (Thermo Fisher Scientific, Cat # G8100) and electrophoresed for 13 mins on the 1-2% agarose gel setting ...
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bioRxiv - Microbiology 2020Quote: ... actively growing Zm6 culture was incubated with SYTO™ 9 Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific) at a concentration of 0.5 or 11 μM for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA extraction was performed using the commercial MagMAX™ CORE Nucleic Acid Purification Kit (Thermo Fisher Scientific). The manual extraction technique has been validated by the Molecular Microbiology Laboratory at Feevale University.
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bioRxiv - Molecular Biology 2020Quote: ... we used 20-30 ng of cDNA and the reaction mixture with SYBR Green nucleic acid staining (Invitrogen) on a CFX96 of CFX384 Real-Time systems (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested by centrifugation and total nucleic acid was purified using GeneJET Genomic DNA purification kit (ThermoFisher) according to the manufacturer’s protocol but omitting the RNAse A treatment ...
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bioRxiv - Cancer Biology 2019Quote: ... or ssDNA M13 primer was labelled using the Ulysis Alexa Fluor 647 nucleic acid labeling kit (Thermo Fisher) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Cells were stained in FACS Staining buffer containing 0.5 µM of SYTOX Green nucleic acid stain solution (Invitrogen) and then analyzed using a BD Accuri C6 flow cytometer instrument (BD Biosciences ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Blot was developed for biotin detection using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Scientific, Waltham, MA)
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bioRxiv - Cancer Biology 2022Quote: ... transfection was performed: Nucleic acid–Lipofectamine 2000 complexes were prepared according to the standard Lipofectamine 2000 protocol (Invitrogen). Four µl of Lipofectamine 2000 reagent in 50 µl Opti-MEM medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Precipitated nucleic acids were washed with ethanol and treated with TURBO DNase per manufacturer’s specification (Life Technologies Corporation).
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bioRxiv - Microbiology 2021Quote: ... DNA was stained with Sytox green nucleic acid stain (5 mM solution in DMSO from Thermo Fisher Scientific) and analyzed on a BD Accuri flow cytometer.
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bioRxiv - Developmental Biology 2020Quote: ... the cells were suspended in FacsMax (amsbio) and stained with SytoxTM green nucleic acid stain (1:100000, Invitrogen) and Hoechst (1:5000 ...
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bioRxiv - Pathology 2020Quote: ... RNA was isolated from these cores using the RecoverAll Total Nucleic Acid Isolation Kit (Ambion, cat. no. 1975). 3SEQ libraries were prepared according to Beck et al ...
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bioRxiv - Genetics 2020Quote: ... and in some cases day 5 (120 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of the biotinylated probe was done using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher 89880).
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bioRxiv - Microbiology 2020Quote: ... were harvested in an Eppendorf tube and resuspended in 0.5 mL NaCl (0.85%) containing SYTO9 green-fluorescent nucleic acid stain (5 nM final concentration, Invitrogen). Following incubation in the dark for 5 min ...
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bioRxiv - Pathology 2021Quote: ... Cell viability was recorded by either fluorescence intensity of 0.1% SYBR green I nucleic acid staining solution (Invitrogen) for attaching cells or Cell Titer-Blue reagent (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... denaturing northern blots were essentially carried out according to Chemiluminescent Nucleic Acid Detection Module Kit (Thermofisher cat# 89880). Essentially ...
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bioRxiv - Developmental Biology 2019Quote: ... Nucleic acid concentration and quality were determined using the Qubit 3.0 fluorometer and Nanodrop 2000 (Thermofisher, Waltham, MA). Generation of cDNA used a consistent starting amount of RNA across all embryos (100 ng) ...
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bioRxiv - Genetics 2020Quote: ... blotted to a positively charged nylon membrane and developed using the Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher) as recommended by the manufacturer.
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bioRxiv - Molecular Biology 2020Quote: Methods compared in this study include magnetic beads method MagMax™ CORE Nucleic Acid Purificatioin Kit (ThermoFisher™), which was adapted for a manual procedure using magnetic racks ...
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bioRxiv - Microbiology 2021Quote: ... stool and homogenized necropsy specimens using MagMAX ™ Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Nucleic acids in the aqueous fraction were precipitated with ethanol and then treated with DNAse I (Thermo Scientific) for 1 h at 37°C ...
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bioRxiv - Genetics 2022Quote: ... cells were stained with a final concentration of 2.4 µM SYTOX Green nucleic acid stain (ThermoFisher Scientific, 1076273). Measurement was performed on the BD LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... they were stained with 10μl of 1x Syto™ BC Green Fluorescent Nucleic Acid Stain (Thermo Scientific, S34855).
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bioRxiv - Systems Biology 2022Quote: Analysis of Cell Cycle: Cell cycle was analyzed using staining with DAPI nucleic acid stain (Thermofisher, Waltham, MA) in cells following dissociation and fixation in single cell suspension ...
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bioRxiv - Plant Biology 2022Quote: ... bacteria were stained with 10ul of 1x Syto™ BC Green Fluorescent Nucleic Acid Stain (Thermo Scientific, S34855). To further investigate whether Pst DC3000 and P ...
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bioRxiv - Microbiology 2023Quote: ... feathers and environmental samples by using the MagMAX™ CORE Nucleic Acid Purification Kit (Thermo Fisher Scientific™) as part of the robotic Mechanical Lysis Module (KingFisher Flex system ...
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bioRxiv - Plant Biology 2023Quote: ... All nucleic acid extracts were quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and stored at -80 °C until analysis.
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bioRxiv - Bioengineering 2023Quote: ... The amount of encapsulated nucleic acid was determined using the Quant-it™ RiboGreen RNA Assay Kit (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the environmental samples using the MagMAXTM CORE Nucleic Acid Purification Kit (ThermoFisher Scientific™) as part of the robotic Mechanical Lysis Module (KingFisher Flex system ...
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bioRxiv - Plant Biology 2023Quote: ... The biotin signals on the membrane were detected using the chemiluminescent nucleic acid detection module (Thermo Fisher, 89880).
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid concentrations were measured with Qubit 3.0 using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). The NGS library preparation was carried out using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB ...