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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Nucleic Acid Electrophoresis Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid concentrations were measured with Qubit 3.0 using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). The NGS library preparation was carried out using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Biophysics 2023Quote: ... cells were stained with PKMito Orange probe (Spirochrome) and SYBR™ Gold Nucleic Acid Stain (Thermo Fisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total RNA from saliva was extracted with MagMAX CORE Nucleic Acid Purification kits (Applied Biosystems, Foster City, CA). Micro-RNAs were detected using Illumina Hiseq x Ten (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... the automated KingFisher system was used with the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (ThermoFisher Scientific, A42356). Sample amount used for DNA extraction ...
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bioRxiv - Immunology 2023Quote: We separated 30 µg of proteins from pellets and supernatants of each AWF using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Tricine; 10% polyacrylamide stacking gel with 20% polyacrylamide separating gel, Novex by Life Technologies, Carlsbad, CA, USA). Electrophoretic separation was performed at 100 V for 110 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... The treated samples were subjected to SDS-polyacrylamide gel electrophoresis on Bolt™ 12% Bis-Tris Plus Protein Gels (Invitrogen). The separated protein samples were then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the resulting 15 uL mixture was separated by gel electrophoresis with a 15% polyacrylamide TBE-urea gel (Invitrogen EC68852BOX) at 200 V for 30 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were visualised under UV light after electrophoresis in 1% Agarose gels containing SYBR Safe DNA gel stain (Invitrogen). Successful amplifications were purified using the GeneJET PCR Purification Kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... of cell extracts was separated by NuPAGE gel electrophoresis (4-12% Midi 1.0 mm Bis-Tris precast gels, Life technologies) and electrotransferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were boiled and reduced in a mixture containing NuPAGE® Sample Reducing Agent (10X) and 4X Protein Loading Buffer before being resolved by SDS gel electrophoresis on 4-20% Tris/glycine gels (Invitrogen). Proteins were transferred onto a nitrocellulose membrane utilizing a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: ... The amplified fragments were analyzed by electrophoresis in 2% agarose gels with SYBR Safe DNA gel stain (Invitrogen, Carlsbad, CA), and was observed in an Enduro™ GDS Touch transilluminator (Labnet International Inc ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes and separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) on NuPAGE 4-12% Bis-Tris Gel (ThermoScientific) in 1x Bolt MES SDS Running Buffer (Invitrogen) for 60 minutes at 130V.
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bioRxiv - Biochemistry 2021Quote: ... Reaction products were separated by gel electrophoresis on 4-12% Bis-Tris gradient gels using MES SDS running buffer (Invitrogen) and stained with Flamingo fluorescent protein stain (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Gel electrophoresis was performed using 4-12% NuPAGE Bis-Tris gels according to manufacturer’s protocols (NuPAGE system, Thermo Fisher Scientific). Protein gel bands were excised ...
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bioRxiv - Genomics 2019Quote: ... Digestion and ligation efficiencies were assessed by gel electrophoresis on a 0.9% agarose gel and also by quantitative PCR (SYBR green, Thermo Fisher).
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bioRxiv - Biochemistry 2020Quote: Purified rFIX samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 4-12% Bis-Tris Bolt acrylamide gel (Invitrogen) in 1x MES running buffer (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... The PCR products were subjected to agarose gel electrophoresis and purified using the GeneJET Gel Extraction Kit (Thermo Scientific™). Then the ORFs were cloned into the pDONR221 vector in presence of BP clonase (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: Protein samples in 1X SDS loading buffer/100 mM dithiothreitol (DTT) were resolved by gel electrophoresis on acrylamide NuPAGE Novex 4-12% Bis-Tris Protein Gels (Invitrogen) with NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Twenty μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% or 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF or P PVDF membranes (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... gondii parasites were separated by polyacrylamide gel electrophoresis (PAGE) in precast NuPAGE (4-12% or 12%) or NativePAGE (4-16%) gels (ThermoFisher) according to the manufacturer’s instructions with minor modifications (detailed in the SI) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The purified RNA samples were prepared for gel electrophoresis by adding 1 μl loading dye (gel loading buffer II, Invitrogen) followed by incubation at 95°C for 5 minutes ...
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bioRxiv - Biophysics 2022Quote: ... 12% polyacrylamide-gel electrophoresis (PAGE) gels in 1x TBE running buffer and were visualized by staining with SYBR Gold II (Invitrogen) stain or fluorescence imager.
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bioRxiv - Neuroscience 2020Quote: ... PCR products were purified by preparative agarose gel electrophoresis and extracted with GeneJET Gel Extraction Kit purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were resolved by gel electrophoresis using the NuPage Bis-Tris 4-12% precast gel system according to the manufacturer’s instructions (Life Technologies). Western blot transfer was carried out according to the manufacturer’s instructions with the SureLock X-cell system (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of DNA extracts were assessed for quantity and quality by gel electrophoresis using 0.8% (w/v) agarose gels and a Qubit® 2.0 Fluorometer (Invitrogen, USA), using high-sensitivity reagents.
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bioRxiv - Microbiology 2021Quote: ... using AccuStart™ II PCR ToughMix (QuantaBio) and subsequent gel electrophoresis on a 1.5% agarose gel with Invitrogen 100 bp DNA Ladder (ThermoFisher Scientific).
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bioRxiv - Biophysics 2021Quote: The purified proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% Bis-Tris NuPAGE gels (Invitrogen). Prior to SDS-PAGE analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... boiled for 5 min and size-fractionated by gel electrophoresis on NuPAGE 10% Bis-Tris 1.5-mm gels (Life Technologies) in 2-(N-morpholino ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of samples (50-100 µg) were resolved by SDS-polyacrylamide gel electrophoresis in 3–8% Tris-Acetate gels (Thermofisher). Gels were wet-transferred in 1x NuPAGE Transfer Buffer (Thermofisher ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were supplemented with 4 μL of 6x loading buffer (30% glycerol, bromphenol blue, xylene cyanol) and were resolved by polyacrylamide gel electrophoresis in 4-12% TBE Gels (Invitrogen) in 1%TBE buffer at 100 V for 45 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... one cDNA fraction corresponding to 70–200 nt was gel purified after 6% TBE-urea gel electrophoresis (Thermo Fisher Scientific). After the final PCR step ...
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bioRxiv - Cell Biology 2019Quote: ... Mixed protein eluates were separated by one-dimensional gel electrophoresis on a 4–12% NuPage Novex Bis–Tris gel (Invitrogen), stained with the Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... We analyzed 40 μg of protein by SDS-polyacrylamide gel electrophoresis on an 8% Tris-Glycine gel (Thermo Fisher Scientific) and transferred overnight at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... was separated by polyacrylamide gel electrophoresis with lithium dodecyl sulfate (SDS-PAGE) on NuPAGE 4%–12% Bis-Tris Gel (Invitrogen). For the analysis of the GFP-tagged proteins ...
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bioRxiv - Developmental Biology 2019Quote: ... 50-100 μg of total protein extracts were solubilized in SDS-sample buffer by boiling at 95°C for 5 minutes and analysed by SDS polyacrylamide gel electrophoresis (4-12% NuPAGE gel; Invitrogen). Western blotting was performed using antibodies against Vasa (rat ...
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bioRxiv - Cell Biology 2020Quote: A total of 20 μg of protein was processed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a 4-12% Bis-Tris Mini-gel (Invitrogen) and the 3-(N-morpholino ...
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bioRxiv - Molecular Biology 2021Quote: ... with 10% β-mercaptoethanol at 70°C for 10min and separated by gel electrophoresis (NuPAGE 4-12% Bis-Tris gel with 1X MES running buffer, ThermoFisher). Gels were transferred to nitrocellulose membranes (ThermoFisher) ...
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bioRxiv - Genetics 2021Quote: ... Digestion and ligation efficiencies were assessed by gel electrophoresis on a 0.9% agarose gel and by quantitative PCR (SYBR green, Thermo Fisher).
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bioRxiv - Neuroscience 2020Quote: ... SDS-resistant and irreversibly assembled protein conjugates were visualized using Novex NuPAGE 12% bis-tris SDS–PAGE (polyacrylamide gel electrophoresis) gels (Invitrogen) run at 4°C in a NuPAGE MES SDS running buffer (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Fourteen μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF membranes (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... tRNAs were ligated to a Cy3-labelled fluorescent stem-loop RNA/DNA oligonucleotide and subjected to gel electrophoresis using a 10% TBE-UREA denaturing-polyacrylamide gel (Invitrogen). tRNA was visualized using a Licor Odyssey imaging system.
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bioRxiv - Immunology 2023Quote: ... The PCR products were subjected to agarose gel electrophoresis and visualized by staining with SYBR Safe DNA Gel stain (Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: ... Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting were performed using pre-cast NuPAGE Bis-Tris 4 - 12% mini-gels (Invitrogen) with 1X MOPS buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and quality was assessed by electrophoresis with 2% Agarose EX-gels using the E-Gel iBase (Invitrogen, Grand Island, NY). Fluidigm Access Array was used to generate 16S rRNA gene amplicons ...
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bioRxiv - Microbiology 2023Quote: ... Equal concentrations of protein were prepared under reducing conditions and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (4–20% Tris Glycine 1.0mm Mini Protein Gels from Invitrogen XP04200BOX). Gels were transferred to PVDF membranes using the iBlot™ 2 transfer stacks (ThermoFisher IB24002) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of each sample were subjected to Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS/PAGE) using precast 10% Bis-Tris NuPage Gels (Invitrogen), transferred onto PVDF membranes ...
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bioRxiv - Genomics 2023Quote: ... DNA quality was assessed with agarose gel electrophoresis (1% TBE gel) and extracts were quantified with Qubit v2.0 (Life Technologies). Double-digest restriction site-associated DNA (ddRAD ...
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bioRxiv - Microbiology 2023Quote: ... The digestion of DNA was determined by agarose gel electrophoresis and visualized by SYBR Safe DNA gel stain (Invitrogen, USA). The reaction samples of V2c or its variants were scaled up for Western blotting analysis ...
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bioRxiv - Cell Biology 2023Quote: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 8% or 10% acrylamide gels and BOLT MES (Invitrogen) or Tris-glycine SDS running buffer ...