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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Human cells were maintained in DMEM (Invitrogen) with 10% FBS (Atlanta Biologicals ...
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bioRxiv - Physiology 2024Quote: Human aortic smooth muscle cells (HASMCs, ThermoFisher) were cultured in smooth muscle cell media (ScienCell ...
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bioRxiv - Neuroscience 2020Quote: ... iPSC colonies that exhibited embryonic stem cell (ESC)-like morphologies were picked under a stereomicroscope for expansion and maintenance in Essential 8™ medium (Gibco™, A1517001).
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bioRxiv - Cancer Biology 2021Quote: ... HEK293 was grown in DMEM (Thermo Fisher, USA, Cat# 11995065) supplemented with 10% FBS (HyClone ...
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bioRxiv - Cancer Biology 2020Quote: ... VCaP and HEK293 were grown in DMEM with Glutamax (Gibco); LNCaP ...
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bioRxiv - Physiology 2022Quote: ... HEK293 were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; ...
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bioRxiv - Neuroscience 2023Quote: ... Stable HEK293 lines were generated by lipofection (Lipofectamine 2000, ThermoFisher) of ∼80% confluent cells with 2 µg of linearized expression plasmid overnight according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 transfections were performed with Lipofectamine 2000 reagent (ThermoFisher, #11668019) according to standard procedures and the cells expressing SorLA mutants and TNFα-GFP (or GFP ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 in Dulbecco’s modified Eagle Medium (DMEM, Life Technologies #11965118); T-47D in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 (ATCC CRL-1573) and HEK293T (Thermo Fisher Scientific NC0260915) cells were maintained in DMEM + GlutaMAX (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC; CRL-1573™) were cultured in complete DMEM (cDMEM) in Nunc EasYFlask T75 cell-culture flasks (Thermo Scientific; cat # 156499) and housed in CO2 incubator (5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... Nerve Growth Factor Receptor (NGFR) (ThermoFisher Scientific, Carlsbad, CA), samples were diluted 1:2 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-oestrogen receptor alpha (Thermo Fisher Scientific, clone SP1), anti-progesterone receptor (DAKO ...
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bioRxiv - Neuroscience 2022Quote: ... mouse transferrin receptor – TfR (1:250, ThermoFisher 13-6800), rabbit ATG9A (1:200 ...
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bioRxiv - Biochemistry 2020Quote: ... Purified receptors (β2AR-Δ5-Cys271+Trp135)and mBBr (Invitrogen) were mixed at the same molarity in LMNG buffer (not containing CHS ...
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bioRxiv - Cancer Biology 2021Quote: ... with Fc Receptor Binding Inhibitor (Thermo Fisher Scientific, MA) for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Transferrin Receptor (TfR) H68.4 1:7500 (ThermoFisher 13-6800); Flotillin 1:1000 (CST #3253) ...
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bioRxiv - Cell Biology 2020Quote: ... Transferrin Receptor/CD71 monoclonal antibody (clone H68.4, ThermoFisher Scientific) was used at 1:200 for immunofluorescence ...
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bioRxiv - Immunology 2024Quote: ... Fcγ receptors were blocked using murine αCD16/CD32 (ThermoFisher), human FcR Blocking (Miltenyi Biotec) ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... or leptin receptor (Cat#: PA1-053, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... [Mouse] anti-glucocorticoid receptor (1:500, ThermoFisher, MA1-510), [Chicken] anti-GFAP (1:1000 ...
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bioRxiv - Physiology 2020Quote: ... human embryonic kidney cells (HEK-293H cells, Thermo Fisher Scientific) were cultured in Dulbecco’s modified Eagle’s medium + GlutaMAX-I + 10% fetal calf serum (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2020Quote: ... Human cells were labeled with cell tracker (Deep Red, Invitrogen) at a final concentration of 1 µM for 30 minutes as previously described 57,58 ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged ChElp2 was pulled-down with Dynabeads™ His-Tag (Thermo Fisher Scientific, Massachusetts, USA) and the subsequent western blot analyses were performed with anti-His (Sigma ...
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Cell Biology 2020Quote: ... Human PAR4 containing an N-terminal V5-epitope were stably expressed using the HEK293 Flp-In T-Rex cells according to the manufacturer’s protocol (Invitrogen). The concentration of tetracycline was titrated (700 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were transfected with either NSUN6 wild-type and the miCLIP-mutant construct using Lipofectamine 2000 (Life Technologies) and harvested 24 hours later.
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bioRxiv - Molecular Biology 2020Quote: ... GFP-N was immunoprecipitated from two independent HEK293 cell pellets using 5μg of anti-GFP antibody (Life Technologies G10362). A total of 2% input material was obtained for size-matched control libraries (SMI ...
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bioRxiv - Genetics 2019Quote: HEK293 cells (CRL-1573, ATCC, VA, USA) were cultured in high-glucose DMEM (Cat. No. 11965092, Life Technologies, USA) supplemented with 10% FBS at 37 and 5% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... The HEK293 cells were transfected with 100 ng/well of pGL3-Fluc plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol at around 75-90% confluency in a 96 well plate ...
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides from HEK293 and HeLa cells were respectively injected into LTQ-Orbitrap Velos and Q Exactive (Thermo Fisher) onto a 75 μm i.d ...
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bioRxiv - Microbiology 2020Quote: The full length CPSF6 protein was expressed in a suspension-adapted HEK293 cell line (Expi 293F, Thermo Fisher Scientific) by transfection of expression plasmid using ExpiFectamine 293 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney (HEK293) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L Glucose and GlutaMAX™ (Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: HEK293 freestyle cells were transfected with this expression construct in combination with pBu-BirA using 293Fectin (Thermo fisher scientific) transfection reagent at 1μg DNA/10^6 cells ...
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bioRxiv - Neuroscience 2022Quote: ... which were used to directly transfect HEK293-Kir2.1 cells with the linear product using lipofectamine 3000 (Thermo Fisher Scientific). For patch clamp characterization in HEK293A cells ...
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bioRxiv - Developmental Biology 2022Quote: ... HEK293E or HEK293-6E cells were maintained in suspension cultures in Freestyle 293 Expression Media (Gibco, Waltham, MA, USA) supplemented with heat-inactivated fetal calf serum (1% ...
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bioRxiv - Molecular Biology 2019Quote: ... cell or nuclear lysates (400 ug) prepared from HEK293 or HDFs to Dynabeads Protein A (10001D, Thermo Fisher Scientific) that were prebound with 2–3μg of indicated antibody and incubated overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... we performed immunoprecipitation of CT-DNA stimulated HEK293 cells using the antibody to IRF3 pS386 crosslinked to Dynabeads (ThermoFisher) overnight at 4 C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively.
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bioRxiv - Biochemistry 2021Quote: ... The construct with a N-terminal 8xHis-TwinStrep-GFP-tag were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in black 96 well plates with transparent bottoms ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting vector was transiently transfected into HEK293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific # A14525). After enhancement ...
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bioRxiv - Neuroscience 2020Quote: ... were subcloned into the polylinker of the pcDNA3.1 vector (Invitrogene, USA) by standard techniques for transiently transfecting HEK293-F cells (ThermoFisher). Cells were transiently transfected with the plasmids containing the desired GABAAR subunit cDNAs (α ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cell lines were transfected with pOG44 and pcDNA5/FRT/TO plasmids harboring respective FLAG constructs using Lipofectamine3000 (Invitrogen) as transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney line 293 (HEK293) wildtype and HTT-null cells were grown in Dulbecco’s modified Eagle’s medium (Thermofisher #41965) supplemented with 10% heat-inactivated fetal bovine serum (ThermoFisher #10500064) ...
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bioRxiv - Neuroscience 2024Quote: ... DRG neurons or HEK293 cells were incubated with 5 μM Fura-2 AM and 0.2% Pluronic (Thermo Fisher Scientific) (prepared from a 200 mg/ml stock solution in DMSO ...