Labshake search
Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... HEK293 cells were seeded and grown on poly-lysine-coated Lab-Tek II eight-well chamber slides (NUNC) overnight and transfected with plasmid DNA using the Lipofectamine™ 3000 Transfection System (Invitrogen ...
-
bioRxiv - Genomics 2023Quote: ... sgRNA-transduced HEK293-idCas9 cells were cultured in high glucose DMEM medium supplemented with L-Glutamine (Gibco 11965092) and 10% FBS ...
-
bioRxiv - Biochemistry 2022Quote: Lentivirus was made by transfecting HEK293 cells with ViraPower packaging plasmids (pLP1, pLP2, and pLP/VSVG) from Invitrogen along with the viral vector using JetPrime ...
-
bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 105 HEK293 cells were seeded in flat-bottom 24-well plates (Thermo Fisher Scientific or FALCON) and incubated overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293S GnTI− cells (ATCC CRL-3022) were grown in suspension at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
-
bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293) cells obtained from ATCC (Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Biophysics 2024Quote: ... the HEK293 cells were incubated with 1 μM Alexa Fluor-647 conjugated α-bungarotoxin (BTX-647; Life Technologies) at 4°C for 1 hr ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid was transfected into HEK293 cells at 70% confluency using Genejuice (Fisher Scientific 70-967-3) at a 1:3 ratio (DNA ...
-
Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: The HEK293 Flp-In T-REx cell line was obtained from Life Technologies (Invitrogen catalogue number R780-07). HEK293 and HCT-116 cells (isolated from the colon of an adult male ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were pre-incubated with anti-Fc receptor antibody (Thermal Fisher Scientific, Cat. No. 14-9161-73) at 1:20 ratio to block Fc receptor before staining ...
-
bioRxiv - Microbiology 2021Quote: ... JCRB #1818) cells expressing the ACE2 or ACE and TMPRSS2 receptors respectively were cultured in DMEM (Gibco) supplemented with 5 % FBS (Fetal Bovine Serum) ...
-
bioRxiv - Developmental Biology 2022Quote: ... and the cell pellet was resuspended in Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen, 14-9161-73) diluted 1:100 in MACS buffer and incubated for 10 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with pCMV6 vectors bearing WT or mutant receptors using Lipofectamine 3000 transfection reagent (Invitrogen). Following 24 h culturing ...
-
bioRxiv - Bioengineering 2024Quote: ... the cells were pre-treated with Fc receptor binding inhibitor polyclonal antibody (eBioscience™, Thermo Fisher Scientific) for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked by incubating cells with 1:50 anti- mouse CD16/CD32 (Fc block, Invitrogen) for 10 minutes at 4°C ...
-
bioRxiv - Microbiology 2020Quote: rCEACAM-His was attached to nickel dynabeads (DB; Dynabead His-tag pull-down & isolation, Invitrogen) following standard protocols ...
-
bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
-
bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
-
bioRxiv - Cell Biology 2023Quote: ... The plasmid pcDNA4-B–hVMP1–V5/His was obtained by inserting the human VMP1 sequence (NM_030938.5) into pcDNATM4/V5-HisB (Invitrogen catalog number V861-20). pLenti–hVMP1–FLAG was obtained by inserting the oligonucleotide MCS-flag into pENTR1A (Addgene Plasmid #17398) ...
-
bioRxiv - Molecular Biology 2020Quote: ... His-tagged hAgo2 was purified from Sf9 cells using a baculovirus system (Thermo Fisher). Cells were lysed in Lysis Buffer (300mM NaCl ...
-
bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
-
bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
-
bioRxiv - Developmental Biology 2020Quote: ... 40 ng/ml insulin-like growth factor-1 (Pepro Tech) and penicillin-streptomycin (Invitrogen). After 1 day of culture ...
-
bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 488 donkey anti-mouse immunoglobulin G (IgG) (Invitrogen, Carlsbad, CA; #A-11001); Alexa Fluor 568 goat anti-rabbit IgG (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-rabbit or anti-mouse immunoglobulin-G labelled with Alexa 488 (1:200; Invitrogen). BodiPy 493/503 (ThermoFisher Cat ...
-
bioRxiv - Biophysics 2020Quote: ... DyLight 633 goat anti-rabbit immunoglobulin G (IgG) highly cross adsorbed (PN35563, Thermo Scientific), 1:200 ...
-
bioRxiv - Biophysics 2020Quote: ... They were cultured in DMEM with decreasing concentrations of low immunoglobulin FBS (Life Technologies) down to 0.5% ...
-
bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488-conjugated goat anti-mouse immunoglobulin G (IgG) (RRID: AB_2536161; Invitrogen, #A28175), Alexa Fluor 488-conjugated goat anti-rabbit IgG (RRID:AB_143165 ...
-
bioRxiv - Biochemistry 2021Quote: Human embryonic kidney HEK-293FT cells (Invitrogen) were cultivated in Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10ng/ml human stem cell factor (Invitrogen) and 10 nM endothelin-1 (Bachem) ...
-
bioRxiv - Cell Biology 2020Quote: Human embryonic kidney 293 cells (ThermoFisher, R70507), human cervical cancer HeLa cell line (ATCC) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human cells were maintained in DMEM (Invitrogen) with 10% FBS (Atlanta Biologicals ...
-
bioRxiv - Neuroscience 2023Quote: Human Umbilical Vein Endothelial Cells (HUVEC, Invitrogen) were maintained as per manufacturer instructions on uncoated tissue culture plates ...
-
bioRxiv - Physiology 2024Quote: Human aortic smooth muscle cells (HASMCs, ThermoFisher) were cultured in smooth muscle cell media (ScienCell ...
-
bioRxiv - Neuroscience 2020Quote: ... iPSC colonies that exhibited embryonic stem cell (ESC)-like morphologies were picked under a stereomicroscope for expansion and maintenance in Essential 8™ medium (Gibco™, A1517001).
-
bioRxiv - Cancer Biology 2021Quote: ... HEK293 was grown in DMEM (Thermo Fisher, USA, Cat# 11995065) supplemented with 10% FBS (HyClone ...
-
bioRxiv - Cancer Biology 2020Quote: ... VCaP and HEK293 were grown in DMEM with Glutamax (Gibco); LNCaP ...
-
bioRxiv - Physiology 2022Quote: ... HEK293 were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; ...
-
bioRxiv - Neuroscience 2023Quote: ... Stable HEK293 lines were generated by lipofection (Lipofectamine 2000, ThermoFisher) of ∼80% confluent cells with 2 µg of linearized expression plasmid overnight according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293 transfections were performed with Lipofectamine 2000 reagent (ThermoFisher, #11668019) according to standard procedures and the cells expressing SorLA mutants and TNFα-GFP (or GFP ...
-
bioRxiv - Cancer Biology 2023Quote: ... HEK293 in Dulbecco’s modified Eagle Medium (DMEM, Life Technologies #11965118); T-47D in Roswell Park Memorial Institute (RPMI ...
-
bioRxiv - Molecular Biology 2024Quote: HEK293 (ATCC CRL-1573) and HEK293T (Thermo Fisher Scientific NC0260915) cells were maintained in DMEM + GlutaMAX (Gibco) ...
-
bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC; CRL-1573™) were cultured in complete DMEM (cDMEM) in Nunc EasYFlask T75 cell-culture flasks (Thermo Scientific; cat # 156499) and housed in CO2 incubator (5% CO2 ...
-
bioRxiv - Neuroscience 2021Quote: ... Nerve Growth Factor Receptor (NGFR) (ThermoFisher Scientific, Carlsbad, CA), samples were diluted 1:2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... anti-oestrogen receptor alpha (Thermo Fisher Scientific, clone SP1), anti-progesterone receptor (DAKO ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse transferrin receptor – TfR (1:250, ThermoFisher 13-6800), rabbit ATG9A (1:200 ...
-
bioRxiv - Biochemistry 2020Quote: ... Purified receptors (β2AR-Δ5-Cys271+Trp135)and mBBr (Invitrogen) were mixed at the same molarity in LMNG buffer (not containing CHS ...
-
bioRxiv - Cancer Biology 2021Quote: ... with Fc Receptor Binding Inhibitor (Thermo Fisher Scientific, MA) for 20 minutes ...